BIOREpository (Faculty of Biology, University of Belgrade)
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Sustainability Assessment of Brown Trout Populations in Serbia (Central Balkans) Using the Modified ESHIPPO Model
No full textIn the inland waters of the Balkans, many brown trout populations have been severely depleted. Therefore, identifying potential threats to their continued survival and ranking populations based on their biological and evolutionary importance enables setting conservation priorities. To assess the sustainability of the brown trout populations in the territory of Serbia (central Balkans), a modification of the ESHIPPO model was performed. The main modification involves incorporating the investigated populations’ genetic structure into the model. Therefore, the new ESHIPPOsalmo model includes an analysis of biological parameters and the impact of multiple factors, including habitat alterations, invasive species, pollution, human population growth, and over-exploitation. In order to investigate individual levels of influence of the model’s analyzed parameters, a combination of supervised and unsupervised machine learning methods was used. The structure of the model is based on general and easily measurable indicators, which enables its application in any salmonid river in the world. By evaluating the parameters of the ESHIPPOsalmo model, we were able to establish that, of the analyzed populations from 46 localities, 37% have a moderate level of sustainability, 43% low, and 20% critically low.M222.342391
Extraction, Characterization, and In Vitro Biological Activity of Polyphenols from Discarded Young Fig Fruits Based on Deep Eutectic Solvents
No full text(1) Background: Discarded young fig fruits (DYFFs) result in a waste of resources, such as sparse fruits and residual fruits, and there has been no research on the relationship between phenolic compounds and biological activity in DYFFs (2) Methods: Different deep eutectic solvents (DESs) and 80% ethanol were used to prepare DYFF extracts, and polyphenol extraction efficiency and bioactivities in the DYFFs extracts were compared. (3) Results: More than 1700 phytochemicals were identified in DYFFs, and thirteen of these typical phenolic compounds were analyzed quantitatively; chlorogenic acid, rutin, luteolin 8-C-glucoside, and epicatechin are the main polyphenols in DYFFs, especially chlorogenic acid with 2720-7980 mg/kg. Ferulic acid, caffeic acid, epicatechin, (+)-catechin, luteolin 8-C-glucoside, rutin, hesperetin, and chlorogenic acid showed different degrees of correlation with in vitro antioxidant activity. Moreover, the highest total phenol content found in the extracts of ChCl-Ethylene glycol (Choline chloride:Ethylene glycol = 1:2) was 8.88 mg GAE/g DW, and all quantitatively analyzed phenolic compounds had high levels in various DESs and 80% ethanol. The 80% ethanol and Choline chloride (ChCl) solvent system showed the greatest antioxidant properties, and the Choline chloride-Urea (Choline chloride: Urea = 1:2) extract of DYFFs exhibited the highest inhibitory activity. (4) Conclusions: DESs have demonstrated potential as promising green solvents, especially the ChCl solvent system, which facilitates the extraction of polyphenols.M21a7.0108413
AdeABC, AdeFGH, and AdeIJK efflux pumps as key factors in tigecycline resistance of Acinetobacter baumannii: a study from Western Balkan hospitals
No full textThe present study investigated the role of resistance-nodulation-cell division (RND) efflux pumps in tigecycline resistance of Acinetobacter baumannii clinical isolates recovered from three Western Balkan countries (Serbia, Bosnia and Herzegovina and Montenegro).M224,
Overview of the database on conservation actions on threatened plant species in Europe
No full textp. 346M3
LncRNAs Involved in Antioxidant Response Regulation as Biomarkers of Gestational Diabetes: A Study on H19, MALAT1 and MEG3
No full textRecent findings highlighted the potential of long non-coding RNAs (lncRNAs) as novel indicators of gestational diabetes mellitus (GDM), as they demonstrate altered expression in metabolic disorders, oxidative stress (OS) and inflammation (IFM). The aim of this study was to evaluate the diagnostic potential and prognostic significance of the OS/IFM-related lncRNAs H19, MALAT1 and MEG3 in GDM and their correlations with redox status-related parameters. The relative quantification of selected lncRNAs from peripheral blood mononuclear cells (PBMCs) of GDM patients and controls (n = 50 each) was performed by qPCR. The expression levels were tested for correlations with metal ion concentrations, NRF2 expression, activities of glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT), serum thiol content, protein carbonyl level and thiobarbituric acid reactive substances. MALAT1 and H19 were significantly downregulated in GDM patients (p = 0.0095 and p = 0.012, respectively). A correlation was observed between H19 expression and zinc levels in both GDM patients and controls. MALAT1 expression positively correlated with NFE2L2 levels in GDM patients (p = 0.026), while H19 exhibited a positive correlation with GR activity in controls (p = 0.018) and an inverse correlation with SOD activity (p = 0.048). Our data show the disturbance of OS/IFM-lncRNAs in GDM pathogenesis and illustrate the biomarker potential of the analyzed lncRNAs, as well as of certain redox status parameters.M21a6.0131
Winter wheat-associated bacteria and their plant growth-promoting and biocontrol abilities
No full textAbstract Book, p. 114.M3
Synthesis and antimicrobial activity of novel pyrido[2,3-d]pyrimidin-4(1H)-ones, and 2-pyrazoline derivatives
No full textThe synthesis of chalcone 1 allowed the production of a novel group of compounds by linking indole with thiophene moieties. The new series of substituted pyridines 2-8, 2-pyrazolines 9-14, and azepines 15, 16 were synthesized using chalcone 1 as a crucial intermediate. 2-Aminopyridines 2, 3, and 4 were produced by reacting compound 1 with cyanoacetamide, ethyl cyanoacetate, and malononitrile, repectively. The α,βunsaturated ketone 1 underwent conversion to 2-thioxodihydropyridopyrimidinone 5 and 2-hydrazinyl pyridopyrimidinone 6. These compounds served as the initial substrate for the synthesis of compounds 7 and 8. 1-Thiocarbamoyl-2-pyrazoline 9 was synthesized by the cycloaddition reaction between thiosemicarbazide and chalcone. 1-(4-oxo-5H-thiazolyl)-2-pyrazoline 10 and 1-(4-phenylthiazolyl)-2-pyrazoline 11 were synthesized by treating thiocarbamoyl derivative 9 with chloroacetic acid or phenacyl bromide. Condensation of thiazolyl pyrazoline 10 with aromatic aldehydes yielded the arylidenes 12-14. The α,β-unsaturated ketone 1 underwent a reaction with bifunctional agents such as o-phenylenediamine or o-aminophenol, resulting in the formatipn of azepines 15 and 16. The novel compounds were structurally determined with spectral data including FTIR, 1H NMR, 13C NMR, and MS spectroscopy as well as elemental analysis. In addition, the new substances were tested for antibacterial activity versus an array of Gram positive and Gram-negative bacteria, as well as four fungal species. Our findings revealed that 2-pyrazolines 9-14 had remarkable effects when compared to the other compounds.M230.62024
