BIOREpository (Faculty of Biology, University of Belgrade)
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Life Under Artificial Light in a Unique Habitat: Exploring Lampenflora in Ravništarka Cave, Serbia
No full textThe phototrophic component of the lampenflora was studied in two seasons in Ravništarka Cave in Serbia. A distinct and diverse lampenflora developed in the vicinity of artificial lights: green to dark, epilithic to endolithic, dominated by microorganisms to macroorganisms or dominated by Cyanobacteria to green algae. The phototrophic microorganisms studied belong to the Cyanobacteria, Chlorophyta (green algae) and Bacillariophyta (diatoms). Diatoms were characterized with highest number of taxa recorded, while green algae were abundant in the biofilms biomass at many sites. Several dominant genera were identified in each group, but the most distinctive diatom genus was Humidophila, while Leptolyngbya stood out among the Cyanobacteria. Stichococcus was the most species-rich green algae, but Chlorella sp. and Bracteacoccus minor were also found at most sampling sites. We found that many of the dominant genera are reported in lampenflora worldwide, while some, such as Sellaphora, are rarely encountered. The ecological parameters were mostly site-specific, and some also depended on the season of sampling. After redundancy analysis, relative humidity and substrate moisture were statistically significant ecological parameters that correlated positively with the Bacillariophyta. The work represents a significant contribution to the knowledge of the phototrophic community of lampenflora and to the understanding of their development in show caves.M222.211
A new Navicula species (Bacillariophyceae) from Serbia’s thermomineral springs
No full textAbstract BookM6
Life under lighting system in Stopić Cave: phototrophs and fungi on artificial substrates
No full textAbstract BookM6
Valorisation of Sunflower Crop Residue as a Potentially New Source of Bioactive Compounds
No full textReducing agricultural waste through reuse has become one of the most important strategies to minimise impact on the environment—an emerging global issue. Sunflower ranks fourth in the world in the production of vegetable oilseeds and therefore generates large amounts of agricultural waste. The aim of this study was to investigate the phytochemical composition and bioactivity of sunflower crop residues in order to open up new opportunities for waste management. TPC and TFC were determined spectrophotometrically, while the dominant compounds were identified by LC-DAD-ESI-MS as ent-kaur-16-en-19-oic acid (KA) and 6Ac-7OH-dimethylchromone (DMC). Both compounds were present in higher concentrations in the ethyl acetate fraction (245.5 and 16.8 mg/g, respectively) than in the ethanol extract. None of the tested samples showed antimicrobial effects in the microdilution test. DMC showed remarkable antioxidant activity by DPPH, ABTS, FRAP and TRC in vitro assays, while both compounds proved to be promising enzyme inhibitory agents, being particularly efficient in inhibiting anti-neurodegenerative enzymes (IC50 values of DMC and KA were 1.20/1.37 mg/mL and 1.44/1.63 mg/mL for AChE/BChE, respectively) and tyrosinase. The results presented indicate that sunflower crop residues are a good candidate for the extraction of bioactive compounds with potential application in the food, pharmaceutical and cosmetic industries.M213,020611
THE GOLDEN JACKAL (CANIS AUREUS) AS A RESERVOIR AND VECTOR FOR ECHINOCOCCUS SPP.
No full textstr. 102-102M3
Micropropagationand optimisation of in vitro productionof the rare and threatened moss Entosthodonpulchellus (Funariaceae)
No full textThe establishment of axenic cultures and the propagation of the rare moss Entosthodon pulchellus (H. Philip.) Brugués (Funariaceae), as well as the optimisation of its ex situ growth conditions and rapid biomass production were the goals of this study. The results obtained also provide insights into the developmental biology of this moss in in vitro conditions, particularly regarding basal media contents with or without supplements of selected plant growth regulators or sugars, i.e. its nutritional needs. The procedure for establishing axenic cultures of this rare and threatened moss species is described. The optimisation protocol of the growth and production conditions is elaborated. The spore germinability of dried herbarium samples remained rather high in the tested laboratory conditions. Suitability tests on different media types showed KNOP basal media to be the best fit for the rapid biomass production of both secondary protonemata and leafy gametophores, without the addition of sugars or plant growth regulators. However, sugar, namely sucrose, induced rapid and massive protonemal development and can be used when this developmental stage is needed.M231.184
Phylogeography of Y-chromosome haplogroup I-P37.2 in Serbian population groups originating from distinct parts of the Balkan Peninsula
No full textGenetic structure of the contemporary Serbian population was shaped by a long history of turbulent historical and demographical events. The most important migrations of Serbs towards present day Serbia, in the recent history, occurred between the 15th to the 18th century from the regions of Old Herzegovina and Kosovo and Metohija. Previous haplogroup analysis revealed wide spectrum of main haplogroups, among which haplogroup I-P37.2 was the most frequent one in Serbian population groups originating from the Balkan Peninsula. Within this study 464 Serbian males samples from three geographical regions in the Balkan Peninsula inhabited by Serbs: present-day Serbia, regions of Old Herzegovina and Kosovo and Metohija, previously classified as haplogroup I-P37.2, were genotyped using the 22 Y-single nucleotide polymorphisms (Y-SNPs) in order to determine deeper phylogenetic and phylogeographic analysis of haplogroup I-P37.2. Based on SNP typing all samples in the Old Herzegovina and present-day Serbia dataset and 122 out of 128 samples from Kosovo and Metohija were assigned to haplogroup I-L621. Further SNP typing revealed very similar haplogroup distribution in all datasets, with the predominant haplogroup being I-PH908, followed by haplogroup I-Z17855. Analysis within haplogroup I-PH908 distinguished haplogroup I-FT14506 as the most frequent in the Kosovo and Metohija dataset, while haplogroup I-FT16449 was the most frequent in the Old Herzegovina dataset. In the present-day Serbia dataset, occurrence of haplogroups I-FT14506 and I-FT16449 was almost equal, comprising 40.2 % and 34.4 %, respectively. Low level of differentiation, within haplogroup I-PH908, was detected between all datasets, with the lowest one detected between present-day Serbia and Old Herzegovina datasets and highest one between Kosovo and Metohija and Old Herzegovina datasets. Furthermore, median-joining network analysis and shared haplotypes statistics revealed closer genetic relationship between Old Herzegovina and present-day Serbia haplotypes. Results obtained within this study support the thesis that migrations from historical region of Old Herzegovina and geographical region of Kosovo and Metohija, had great contribution on the present-day Serbian population genetic structure. Furthermore, here presented results, gave insight into geographic distribution of detected haplogroups I-Z17855, I-Y4460, I-PH908, I-Y5596, I-Y4882, I-FT14506, I-FT16449 and I-A5913 and analyzed SNPs, enabling further improvement of the geographic resolution of paternal ancestry inference.21a3,51031527
Analysis of TNS3-203 and LRRFIP1-211 Transcripts as Oral Cancer Biomarkers
No full textA recent pan-cancer transcriptome analysis indicated differential activity of alternative promoters of genes TNS3 and LRRFIP1 in head and neck squamous cell carcinoma compared to non-cancerous tissue. The promoters upregulated in head and neck squamous cell carcinoma regulate expression of transcripts TNS3-203 and LRRFIP1-211.M212.715116054
Insights into bioactivity guided chemical profiling of Ziziphus jujuba Mill. fruits wild-growing in Montenegro
No full textJujube (Ziziphus jujuba Mill.) is a highly abundant wild-growing plant in Montenegro. It has been utilized since old times for various bioactive properties by the natives, however its detailed chemical characterization, antimicrobial, antioxidant and cytotoxic potential have not been extensively explored. Herein, we used crud methanol extract and three fractions (methylene chloride, n-butanol and aqueous) to asses bioactive features of fruits from this wild growing edible plant, after which we performed the targeted analysis of phenolic compounds of n-butanol fraction by (UHPLC-DAD-MS/MS). Our antioxidant assays showed the highest radical scavenging potential for n-butanol fraction using DPPH and ABTS methods. As for the antimicrobial activity of extract and three fractions, generally aqueous showed the least promising antibacterial and antifungal properties, whereas methylene chloride, methanol and n-butanol fractions showed quite promising antimicrobial potential. E. coli and S. aureus strains were the most susceptible to the compounds present in the methylene chloride and n-butanol fractions with MIC of 0.01-0.025 mg/mL, and MBC 0.025-0.05 mg/mL, along with MRSA strain, which was the most susceptible to the effects of methanol extract with MIC of 0.10 mg/mL and MBC 0.15 mg/mL. The results of antifungal activity showed lower potential to inhibit growth of pathogenic fungi than bacteria, whereas cytotoxicity assay showed extracts have no effects towards HaCaT skin cell line.The n-butanol fraction had the overall most promising activity, and therefore was subjected to more in depth chemical analysis.M223.4e4136111
