BIOREpository (Faculty of Biology, University of Belgrade)
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Bats as bioindicators: First insight into seasonal metal exposure and immune stress in bats from mining area
No full textBook of Abstracts 73M3
Genotype-sensitive dynamic model of the serotonin presynapse shows complex interplay between synapse metabolites
No full textBook of abstracts: 37M3
Effects of protected areas and linear elements on bat and moth diversity in intensive agricultural landscapes in Serbia
No full textBook of abstracts pp. 170M3
The Role of Root Endophyte Pseudomonas putida A32 in the Protection of Two Pepper Genotypes from Pseudomonas syringae pv. aptata
No full textEndophytes, as an integral part of plants, form unique relationships with their hosts that go beyond classical definitions of symbiosis and influence plant development, immunity, and stress responses. The pepper endophyte strain Pseudomonas putida A32 has several plant growth-promoting properties and increases the tolerance of pepper to drought, but its biocontrol potential is unknown. In this study, we investigated the protective role of P. putida A32 against infection with the pathogenic bacterium P. syringae pv. aptata P21 in two pepper genotypes in laboratory experiments. The percentage of lesion reduction in genotype 26 treated with P. putida A32 was 46.62%. The results showed a significant reduction in hydrogen peroxide and malondialdehyde levels by 29.45 and 20.22%, respectively, in infected genotype 26. The treated but uninfected controls showed a significant increase in superoxide dismutase activity in genotype 26 by 41.26% and ascorbate peroxidase activity in genotype 19 by 40.28% in the treated infected plants. The tolerant genotype 19 was much less dependent on the bacterial treatment under stress conditions than the susceptible genotype 26. Future research will investigate the role of P. putida A32 in the induced systemic resistance of different pepper genotypes to protect against pathogens.M213.153611
Type III Secretion System-Mediated Induction of Systemic Resistance by Pseudomonas marginalis ORh26 Enhances Sugar Beet Defence Against Pseudomonas syringae pv. aptata
No full textThe increasing demand for sustainable agricultural practises has sparked interest in microbes that promote plant immunity. Among these, Pseudomonas species have shown the potential to enhance induced systemic resistance (ISR) in crops. While type III secretion systems (T3SSs) in pathogenic bacteria have been widely studied for their role in local immunosuppression, their function in beneficial Pseudomonas species and on a systemic level remains largely unexplored. We show for the first time that the T3SS of a plant-beneficial Pseudomonas strain induces ISR by root colonisation. T3SS-positive Pseudomonas isolates were applied to the roots of sugar beet (Beta vulgaris L.) and systemic effects on plant immunity were assessed in leaves exposed to the pathogen P. syringae pv. aptata P21. Our results show that P. marginalis ORh26 reduced lesion size and pathogen proliferation in sugar beet leaves. ORh26 activated peroxidase and phenylalanine ammonia-lyase and upregulated NPR1 and MYC2 defence genes. Remarkably, a T3SS-deficient mutant of ORh26 failed to induce these effects. Genomic analysis identified T3SS structural genes and effector proteins, including a pectate lyase and an effector of the HopJ family, that may mediate these responses. This study reveals a previously uncharacterised role of T3SS in the induction of ISR and improves our understanding of plant-microbe interactions.M214.11621141
Seasonal and sex-specific accumulation of some trace elements in Glomeris hexasticha Brandt, 1833 (Myriapoda: Diplopoda: Glomerida) across urban localities
No full textAbstract Book (p. 114)M3
How diverse are Lithobiidae (Chilopoda: Lithobiomorpha) in caves of the Dinaric karst?
No full textAbstract Book (p. 63)M3
The poorly known cave centipede Cryptops illyricus Verhoeff, 1933 (Chilopoda: Scolopendromorpha: Cryptopidae) from caves of the northern Dinarides
No full textAbstract book (p. 41)M3
Acid ceramidase expression and biomarker potential in patients with locally advanced rectal cancer
No full textAcid ceramidase (AC), a pivotal enzyme in sphingolipid metabolism, has been associated with various cancers; however, its specific role in rectal cancer remains poorly understood. This study aimed to explore the clinical significance of AC gene and protein expression in rectal cancer. We analyzed the expression of ASAH1, BAX, and BCL2 through quantitative Real-Time PCR in paired tumor and non-tumor tissue samples obtained from patients with locally advanced rectal cancer (LARC) prior to neoadjuvant chemoradiotherapy. Additionally, serum AC levels and standard biochemical parameters were assessed. We further evaluated ASAH1 expression using RNA-seq data from publicly available TCGA-READ datasets accessed via the UCSC Xena Browser. Two approaches indicated a significant reduction in ASAH1 expression in tumor tissue (p=0.004 and p<0.001, respectively). Receiver operating characteristic curve analysis revealed a modest capacity for ASAH1 expression to differentiate between tumor and non-tumor tissue in LARC patients (AUC=0.652, p=0.042). No correlation was observed between ASAH1 expression and the BAX/BCL2 ratio in tumor tissue, nor with serum AC levels or the CRP-albumin-lymphocyte (CALLY) index. Conversely, serum AC levels exhibited a negative correlation with the BAX/BCL2 ratio (rs=-0.536, p=0.002, FDR-adjusted q=0.021). Furthermore, ASAH1 expression, AC levels, and the CALLY index were not linked to overall survival or treatment response. A key finding of this study is the inverse relationship between serum AC levels and the pro-apoptotic status of tumor tissue, suggesting that circulating AC may provide valuable insights into tumor apoptotic activity. Further large-scale studies are necessary to validate these preliminary findings and elucidate the biomarker potential of AC in rectal cancer.M222.
