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Reply to "Reversible Cerebral Vasoconstriction Syndrome Responsive to Intravenous Milrinone"
No full textResumen no disponibleFil: Hawkes, Maximiliano Alberto. Fleni. Departamento de Medicina Interna; Argentina. Fleni. Departamento de Neurología; Argentina.Fil: Hlavnicka, Alejandro A. Fleni. Departamento de Medicina Interna; Argentina.Fil: Wainsztein, Nestor Adrián. Fleni. Departamento de Medicina Interna; Argentina
Cerebral venous thrombosis in Argentina: clinical presentation, predisposing factors, outcomes and literature review
No full textBackground: Cerebral venous thrombosis (CVT) is a rare medical condition that primarily affects young adults. The clinical spectrum is broad and its recognition remains a challenge for clinicians. Limited information is available on CVT in Argentina. Our goal was to report the results of the first National registry on CVT in Argentina and to compare clinical presentation, predisposing factors and outcomes with other international registries.
Material and method: The Argentinian National Registry on CVT (ANR-CVT) is a multicenter retrospective cohort study comprising patients aged 18 and older with a diagnosis of CVT from January 2015 to January 2019. We evaluated demographics, predisposing factors, clinical presentation, and radiological characteristics (e.g. number of involved sinuses, venous infarction or hemorrhage on CT and MRI scans at admission), therapeutic interventions and functional outcomes at discharge and at 90 days. Our results were compared to a literature review of CVT registries.
Results: Overall, one hundred and sixty-two patients met the inclusion criteria. The mean age was 42 (±17) years; 72% were women. Seventy percent of patients were younger than 50 years. The most common presenting symptom was headache (82%). The transverse sinus was the most common site of thrombosis (70%) followed by the sigmoid sinus (46%). The main predisposing factor in women was contraceptive use (44%), 3% of the events occurred during pregnancy and 9% during the puerperium. Participants 50 years and older had a higher frequency on malignancy related (7.5% vs. 30%, p = 0.0001) and infections (2% vs. 11%, p = 0.001). The modified Rankin Scale (mRS) ≤2 at discharge was 81% and the rate of mortality at discharge was 4%. At 90 days, the mRS≤2 was 93%. When the ANR-CVT was compared with larger registries from Europe and Asia, the prevalence of cancer among patients with CVT was two to five-fold higher (15% vs. 7% and 3%, respectively; p = 0.002 and p < 0.001). Anticoagulation rates at discharge were also higher (94%) compared to registries from Asia (ASCVT - 68%) or Turkey (VENOST - 67%).
Conclusion: Participants in the first ANR-CVT had a low mortality and disability at 90 days. Clinical and radiological characteristics were similar to CVT from other international registries with a higher prevalence of cancer. There was a high variability in treatment adherence to guidelines as reflected by anticoagulation rates (range 54.5%-100%) at discharge.Fil: Pujol Lereis, Virginia Andrea. Fleni. Centro Integral de Neurología Vascular; Argentina.Fil: Alet, Matías Javier. Fleni. Centro Integral de Neurología Vascular; Argentina.Fil: Ciardi, Celina. Clínica La Sagrada Familia; Argentina.Fil: Alemán, Alberto. Instituto Médico de Alta Complejidad; Argentina.Fil: Bandeo, Lucrecia. Hospital Británico; Argentina.Fil: Bonardo, Pablo. Hospital Británico; Argentina.Fil: Cea, Clarisa. Hospital Italiano de Buenos Aires; Argentina.Fil: Cirio, Juan. Clínica La Sagrada Familia; Argentina.Fil: Cossio, Jerónimo. Hospital Ángel Cruz Padilla; Argentina.Fil: Cuculic, María. Hospital Julio Cecilio Perrando; Argentina.Fil: Esnaola, María Martha. Hospital César Milstein; Argentina.Fil: García-Pérez, Fernando. Neuromadryn; Argentina.Fil: Giner, Federico. Hospital Lagomaggiore; Argentina.Fil: Gómez Schneider, Maia Macarena. Sanatorio de los Arcos. Instituto de Neurología y Neurocirugía; Argentina.Fil: Isaac, Cristian. Hospital Dr. Arturo Oñativia; Argentina.Fil: Lepera, Sandra. Hospital General de Agudos J. M. Ramos Mejía; Argentina.Fil: Martínez, Carlos. Hospital Cullen; Argentina.Fil: Martínez-Lorenzín, Román. Hospital Provincial del Centenario; Argentina.Fil: Montes, Mariana. HIGA Gral. San Martín de La Plata; Argentina.Fil: Orzuza, Gabriela. Hospital San Bernardo; Argentina
SWAN-Venule: An Optimized MRI Technique to Detect the Central Vein Sign in MS Plaques
No full textBackground and purpose: Multiple sclerosis lesions develop around small veins that are radiologically described as the so-called central vein sign. With 7T MR imaging and magnetic susceptibility-based sequences, the central vein sign has been observed in 80%-100% of MS lesions in patients' brains. However, a lower proportion ∼50% has been reported at 3T using susceptibility-weighted angiography (SWAN). Our aim was to assess a modified version of SWAN optimized at 3T for sensitive detection of the central vein sign.
Materials and methods: Thirty subjects with MS were scanned on a 3T clinical MR imaging system. 3D T2-weighted FLAIR and optimized 3D SWAN called SWAN-venule, were acquired after injection of a gadolinium-based contrast agent. Patients showing >3 focal white matter lesions were included. The central vein sign was recorded by 2 trained raters on SWAN-venule images in the supratentorial brain.
Results: Twenty patients showing >3 white matter lesions were included. A total of 380 white matter lesions (135 periventricular, 144 deep white matter, and 101 juxtacortical) seen on both FLAIR and SWAN-venule images were analyzed. Overall, the central vein sign was detected in 86% of the white matter lesions (periventricular, 89%; deep white matter, 95%; and juxtacortical, 78%).
Conclusions: The SWAN-venule technique is an optimized MR imaging sequence for highly sensitive detection of the central vein sign in MS brain lesions. This work will facilitate the validation and integration of the central vein sign to increase the diagnostic certainty of MS and further prevent misdiagnosis in clinical practice.Fil: Correale, Jorge. Fleni. Departamento de Neurología. Servicio de Neuroinmunología y Enfermedades Desmielinizantes; Argentina.Fil: Calandri, Ismael Luis. Fleni. Departamento de Neurología. Servicio de Neurología Cognitiva, Neuropsicología y Neuropsiquiatría; Argentina.Fil: Gaitán, María Inés. Fleni. Departamento de Neurología. Servicio de Neuroinmunología y Enfermedades Desmielinizantes; Argentina.Fil: Yáñez, Paulina. Fleni. Departamento de Diagnóstico por Imágenes; Argentina.Fil: Paday Fomenti, María Emilia. Fleni. Departamento de Diagnóstico por Imágenes; Argentina.Fil: Figueiredo E. GE healthcare; Brasil.Fil: Sati P. National Institutes of Health. National Institute of Neurological Disorders and Stroke. Translational Neuroradiology Section (P.S.); Estados Unidos
Feeding difficulties in children and adolescents with spinal muscular atrophy type 2
No full textDisease course of feeding difficulties in spinal muscular atrophy type 2 is not well documented. Disease-modifying therapies rapidly change the trajectory of motor function and survival in spinal muscular atrophy, but effects on co-morbidities like bulbar function are unknown. We analysed data concerning feeding problems and their standard of care treatment in 146 patients with spinal muscular atrophy type 2. Data were collected from two separate cohorts: one single-centre retrospective chart review study from the United Kingdom (London), and one prospective questionnaire-based multicentre study from Italy. Cumulatively feeding difficulties were present in 88 patients (60%) in these 2 cohorts. Median age at onset of problems was 6.5years (range 0-16.5 years). Eighty-two patients (60%) showed periods of underweight according to age adjusted body mass index, and thirty-six patients (25%) showed malnourishment with a significant drop on their weight curves. Enteral feeding was indicated in 23 out of 72 patients in the UK cohort (32%) because of weight loss, oropharyngeal dysphagia or aspiration. Gastrostomy and its placement was generally well tolerated, uncomplicated in 96%, never reversed and performed without Nissen fundoplication in 66% of patients. After gastrostomy chest infections improved in 80% and nutritional status (e.g., Body Mass Index) in 84% of patients. These results show that feeding difficulties are a common problem in spinal muscular atrophy type 2. Treatment strategies should be tailor-made on the symptoms and needs of the individual patient.Fil: Schottlaender, Lucía V. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fleni. Departamento de Neurología; Argentina. University College London. Great Ormond Street Institute of Child Health. NIHR Great Ormond Street Hospital Biomedical Research Centre. Dubowitz Neuromuscular Centre; Reino Unido. Great Ormond Street Hospital Trust; Reino Unido. University College London. Institute of Neurology. Department of Molecular Neuroscience; Reino Unido.Fil: Wadman, Renske I. University College London. Great Ormond Street Institute of Child Health. NIHR Great Ormond Street Hospital Biomedical Research Centre. Dubowitz Neuromuscular Centre; Reino Unido. Great Ormond Street Hospital Trust; Reino Unido. University Medical Center Utrecht. UMC Utrecht Brain Center. Department of Neurology and Neurosurgery; Países Bajos.Fil: De Amicis, Ramona. University of Milan. Department of Food Environmental and Nutritional Sciences.
International Center for the Assessment of Nutritional Status; Italia.Fil: Battezzati, Alberto. University of Milan. Department of Food Environmental and Nutritional Sciences.
International Center for the Assessment of Nutritional Status; Italia.Fil: Brusa, Chiara. University College London. Great Ormond Street Institute of Child Health. NIHR Great Ormond Street Hospital Biomedical Research Centre. Dubowitz Neuromuscular Centre; Reino Unido. Great Ormond Street Hospital Trust; Reino Unido.Fil: Bertoli, Simona. University of Milan. Department of Food Environmental and Nutritional Sciences. International Center for the Assessment of Nutritional Status; Italia. IRCCS Istituto Auxologico Italiano. Department of Endocrine and Metabolic Diseases. Obesity Unit and Laboratory of Nutrition and Obesity Research; Italia.Fil: Davis, Tracey. Great Ormond Street Hospital for Children. Dubowitz Neuromuscular Centre; Reino Unido.Fil: Main, Marion. Great Ormond Street Hospital for Children. Dubowitz Neuromuscular Centre; Reino Unido.Fil: Manzur, Adnan. Great Ormond Street Hospital for Children. Dubowitz Neuromuscular Centre; Reino Unido.Fil: Mastella, Chiara. Mangiagalli e Regina Elena Hospital. Early Habilitation Service; Italia.Fil: Munot, Pinki. Great Ormond Street Hospital for Children. Dubowitz Neuromuscular Centre; Reino Unido.Fil: Imbrigiotta, Nadia. Great Ormond Street Hospital for Children. Dubowitz Neuromuscular Centre; Reino Unido.Fil: Sarkozy, Anna. Great Ormond Street Hospital for Children. Dubowitz Neuromuscular Centre; Reino Unido.Fil: Trucco, Federica. Great Ormond Street Hospital for Children. Dubowitz Neuromuscular Centre; Reino Unido.Fil: Baranello, Giovanni. Great Ormond Street Hospital for Children. Dubowitz Neuromuscular Centre; Reino Unido. Ormond Street Hospital Trust; Reino Unido. Fondazione IRCCS Istituto Neurologico Carlo Besta. Developmental Neurology Unit; Italia.Fil: Scoto, Mariacristina. Great Ormond Street Hospital for Children. Dubowitz Neuromuscular Centre; Reino Unido.Fil: Muntoni, Francesco. Great Ormond Street Hospital for Children. Dubowitz Neuromuscular Centre; Reino Unido
Neuropsychological profile of Alzheimer’s disease based on amyloid biomarker findings results from a South American cohort. Appl Neuropsychol Adult 1–6.
No full textObjective: Increased life expectancy and exponential growth of adults suffering from Alzheimer's disease (AD) worldwide, has led to biomarkers incorporation for diagnosis in early stages. Use of neuropsychological testing remains limited. This study aimed to identify which neuropsychological tests best indicated underlying AD pathophysiology.Methods: One hundred and forty-one patients with MCI (Mild Cognitive Impairment) were studied. A neuropsychological test battery based on the Uniform Data Set (UDS) from the Alzheimer's Disease Centers program of the National Institute on Aging (NIA) was performed and amyloid markers recorded; according to presence or absence of amyloid identified by positive PIB-PET findings, or low CSF Aβ42 levels, patients were separated into MCI amyloid-(n:58) and MCI amyloid + (n = 83) cases.Results: Statistical differences were found in all memory tests between groups. Delayed recall score at thirty minutes on the Rey Auditory Verbal Learning Test (AVLT) was the best predictor of amyloid pathology presence (AUC 0.68), followed by AVLT total learning (AUC 0.66) and AVLT Recognition (AUC 0.59) scores, providing useful cut off values in the clinical setting.Conclusions: Use of neuropsychological testing, specifically AVLT scores with cutoff values, contributed to the correct diagnosis of MCI due to AD in this SouthAmerican cohort.Fil: Clarens, María Florencia. Fleni. Departamento de Neurología. Servicio de Neurología Cognitiva, Neuropsicología y Neuropsiquiatría; Argentina.Fil: Crivelli, Lucía. Fleni. Departamento de Neurología. Servicio de Neurología Cognitiva, Neuropsicología y Neuropsiquiatría; Argentina.Fil: Chrem Méndez, Patricio Alexis. Fleni. Departamento de Neurología. Servicio de Neurología Cognitiva, Neuropsicología y Neuropsiquiatría; Argentina.Fil: Martín, María Eugenia. Fleni. Departamento de Neurología. Servicio de Neurología Cognitiva, Neuropsicología y Neuropsiquiatría; Argentina.Fil: Russo, María Julieta. Fleni. Departamento de Neurología. Servicio de Neurología Cognitiva, Neuropsicología y Neuropsiquiatría; Argentina.Fil: Surace, Ezequiel Ignacio. Fleni. Departamento de Neuropatología y Biología Molecular. Laboratorio de Enfermedades Neurodegenerativas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Campos, Jorge. Fleni. Departamento de Neurología. Servicio de Neurología Cognitiva, Neuropsicología y Neuropsiquiatría; Argentina.Fil: Vázquez, Silvia. Fleni. Centro de Imágenes Moleculares CIM; Argentina.Fil: Sevlever, Gustavo Emilio. Fleni. Departamento de Neuropatología y Biología Molecular; Argentina.Fil: Allegri, Ricardo Francisco. Fleni. Departamento de Neurología. Servicio de Neurología Cognitiva, Neuropsicología y Neuropsiquiatría; Argentina.Fil: Calandri, Ismael Luis. Fleni. Departamento de Neurología. Servicio de Neurología Cognitiva, Neuropsicología y Neuropsiquiatría; Argentina
Transcranial Magnetic Resonance-Guided Focused Ultrasound Thalamotomy in Essential Tremor: A Comprehensive Lesion Characterization
No full textBACKGROUND
Transcranial magnetic resonance-guided focused ultrasound (tcMRgFUS) thalamotomy is a novel and effective treatment for controlling tremor in essential tremor patients.
OBJECTIVE
To provide a comprehensive characterization of the radiological, topographical, and volumetric aspects of the tcMRgFUS thalamic lesion, and to quantify how they relate to the clinical outcomes.
METHODS
In this study, clinical and radiological data from forty patients with medically-refractory essential tremor treated with unilateral tcMRgFUS thalamotomy were retrospectively analyzed. Treatment efficacy was assessed with Clinical Rating Scale for Tremor (CRST). Lesions were manually segmented on T1, T2, and susceptibility-weighted images, and 3-dimensional topographical analysis was then carried out. Statistical comparisons were performed using nonparametric statistics.
RESULTS
The greatest clinical improvement was correlated with a more inferior and posterior lesion, a bigger lesion volume, and percentage of the ventral intermediate nucleus covered by the lesion; whereas, the largest lesions accounted for the occurrence of gait imbalance. Furthermore, the volume of the lesion was significantly predicted by the number of sonications surpassing 52°C.
CONCLUSION
Here we provide a comprehensive characterization of the thalamic tcMRgFUS lesion including radiological and topographical analysis. Our results indicate that the location and volume of the lesion were significantly associated with the clinical outcome and that mid-temperatures may be responsible for the lesion size. This could serve ultimately to improve targeting and judgment and to optimize clinical outcome of tcMRgFUS thalamotomy.Fil: Pineda Pardo, José Ángel. Hospital Universitario HM Puerta del Sur. Centro Integral de Neurociencias; España. Instituto Carlos III Network Center for Biomedical Research on Neurodegenerative Diseases; España.Fil: Urso, Daniele. Hospital Universitario HM Puerta del Sur. Centro Integral de Neurociencias; España.King's College London. Institute of Psychiatry, Psychology and Neuroscience. Neurodegeneration Imaging Group; Reino UnidoFil: Martínez Fernández, Raul.Hospital Universitario HM Puerta del Sur. Centro Integral de Neurociencias ; España. Instituto Carlos III Network Center for Biomedical Research on Neurodegenerative Diseases; España.Fil: del Alamo, Marta. Hospital Universitario HM Puerta del Sur. Centro Integral de Neurociencias; España.Fil: Millar Vernetti, Patricio. Fleni. Departamento de Neurología. Servicio de Movimientos Anormales; Argentina.Fil: Máñez Miró, Jorge U.Hospital Universitario HM Puerta del Sur. Centro Integral de Neurociencias; España.Fil: Hernández Fernández, Frida. Hospital Universitario HM Puerta del Sur. Centro Integral de Neurociencias; España.Universidad Europea de Madrid, Faculty of Biomedical and Health Sciences, Department of Nursing; EspañaFil: de Luis Pastor, Esther. Hospital Universitario HM Puerta del Sur .Radiology Department; EspañaFil: Vela Desojo, Lydia. Hospital Universitario HM Puerta del Sur. Centro Integral de Neurociencias; España.Fil: Obeso, José A.Hospital Universitario HM Puerta del Sur. Centro Integral de Neurociencias; España. Instituto Carlos III Network Center for Biomedical Research on Neurodegenerative Diseases; España.Fil: Rodríguez Rojas, Rafael.Hospital Universitario HM Puerta del Sur. Centro Integral de Neurociencias; España. Instituto Carlos III Network Center for Biomedical Research on Neurodegenerative Diseases; España
Acromegaly and thyroid cancer: analysis of evolution in a series of patients
No full textBackground: Acromegaly is associated with higher morbidity and mortality mainly due to cardiovascular disease. Data on the incidence and evolution of thyroid cancer in acromegaly are controversial. Our objective was to describe the characteristics of a group of acromegalic patients with differentiated thyroid carcinoma (DTC) and analyze their evolution.
Methods: This is a retrospective multicenter study of 24 acromegalic patients with DTC. The AJCC Staging System 8th Edition was used for TNM staging, and the initial risk of recurrence (RR), initial response and response at the end of follow-up (RFU) were defined according to the 2015 ATA Guidelines. As a control group, 92 patients with DTC without acromegaly were randomly included. Statistical analyses were done using SPSS Statistics 20.0.
Results: Median age of patients at diagnosis of acromegaly was 49.5 years (range 12-69). The median delay in diagnosis of acromegaly was 3 years (range 0.5-23). Mean baseline IGF-1 level was 2.9 ± 1.1 ULN. Median age at DTC diagnosis was 51.5 years (18-69). At the moment of diagnosis of DTC, 58.3% of the patients had active acromegaly. Median time from DTC diagnosis to acromegaly control was 1.25 years (0.5-7). Mean DTC tumor diameter of the biggest lesion was 14.6 ± 9.2 mm, being multifocal in 37.5%. All tumors were papillary carcinomas, two cases being of an aggressive variety. Lymph node dissection was performed in 8 out of 24 patients and 62.5% had metastases. Only one patient had distant metastases. Radioiodine ablation was given to 87.5% of patients. Nineteen patients (79%) were stage I, four (17%) stage II and one (4%) stage IVb. Initial RR was low in 87% (21/24), intermediate in 9% (2/24) and high in 4% (1/24) patient. RFU was: 83% (19/23) patients with no evidence of disease, 9% (2/23) with indeterminate response, 4% (1/23) with biochemical incomplete response and 4% (1/23) with structural incomplete response, at a median time of FU of 36.5 months. When comparing RFU between acromegalics and controls no statistically significant differences were found.
Conclusions: Patients with acromegaly and DTC mostly had a low initial RR. When compared with the control group, we found that DTC patients with acromegaly did not have a worse evolution.Fil: Danilowicz, Karina. Hospital de Clínicas José de San Martín; Argentina. Sociedad Argentina de Endocrinología y Metabolismo; Argentina.Fil: Sosa, Soledad. Hospital de Clínicas José de San Martín; Argentina. Sociedad Argentina de Endocrinología y Metabolismo; Argentina.Fil: González Pernas, Mariana Soledad. Sociedad Argentina de Endocrinología y Metabolismo; Argentina. Fleni. Departamento de Neurología. Servicio de Neuroendocrinología; Argentina.Fil: Bamberger, Elizabeth. Sociedad Argentina de Endocrinología y Metabolismo; Argentina. Centro Privado de Endocrinología; Argentina.Fil: Diez, Sabrina Mara. Sociedad Argentina de Endocrinología y Metabolismo; Argentina. Hospital Pirovano. División Endocrinología; Argentina.Fil: Fainstein-Day, Patricia. Sociedad Argentina de Endocrinología y Metabolismo; Argentina. Hospital Italiano de Buenos Aires. Departamento de Endocrinología y Medicina Nuclear; Argentina.Fil: Furioso, Alejandra. Sociedad Argentina de Endocrinología y Metabolismo; Argentina. Hospital Ramos Mejía. División Endocrinología; Argentina.Fil: Glerean, Mariela. Sociedad Argentina de Endocrinología y Metabolismo; Argentina. Hospital Italiano de Buenos Aires. Departamento de Endocrinología y Medicina Nuclear; Argentina.Fil: Guitelman, Mirtha. Sociedad Argentina de Endocrinología y Metabolismo; Argentina. Hospital Carlos G. Durand. División Endocrinología; Argentina.Fil: Katz, Débora Adela. Sociedad Argentina de Endocrinología y Metabolismo; Argentina. Fleni. Departamento de Neurología. Servicio de Neuroendocrinología; Argentina.Fil: Lemaitre, Nicole. Hospital Ángel C. Padilla. División Endocrinología; Argentina.Fil: Lowenstein, Alicia. Hospital Ramos Mejía. División Endocrinología; Argentina.Fil: Del Valle Luna, Mariela. Hospital Ramos Mejía. División Endocrinología; Argentina.Fil: Martínez, María Paz. Hospital Alemán. División Endocrinología; Argentina.Fil: Miragaya, Karina. Hospital Alemán. División Endocrinología; Argentina.Fil: Moncet, Daniel. Hospital Privado de Comunidad. División Endocrinología; Argentina.Fil: Ortuño, María Victoria. Hospital Alemán. División Endocrinología; Argentina.Fil: Pignatta, Analía. Hospital Interzonal San Juan Bautista. División Endocrinología; Argentina.Fil: Ramacciotti, Constanza Fernanda. hospital Privado Universitario de Córdoba. División Endocrinología; Argentina.Fil: Reyes, Adriana. Hospital Ramos Mejía. División Endocrinología; Argentina.Fil: Rogozinski, Amelia Susana. Sociedad Argentina de Endocrinología y Metabolismo; Argentina. Hospital Ramos Mejía. División Endocrinología; Argentina.Fil: Slavinsky, Patricia. Fleni. Departamento de Neurología. Servicio de Neuroendocrinología; Argentina. Sociedad Argentina de Endocrinologia y Metabolismo; Argentina.Fil: Tkatch, Julieta. Sociedad Argentina de Endocrinologia y Metabolismo; Argentina. Hospital Carlos G. Durand. División Endocrinología; Argentina.Fil: Pitoia, Fabián. Hospital de Clínicas José de San Martín; Argentina
The entropic tongue: Disorganization of natural language under LSD
No full textSerotonergic psychedelics have been suggested to mirror certain aspects of psychosis, and, more generally, elicit a state of consciousness underpinned by increased entropy of on-going neural activity. We investigated the hypothesis that language produced under the effects of lysergic acid diethylamide (LSD) should exhibit increased entropy and reduced semantic coherence. Computational analysis of interviews conducted at two different time points after 75 μg of intravenous LSD verified this prediction. Non-semantic analysis of speech organization revealed increased verbosity and a reduced lexicon, changes that are more similar to those observed during manic psychoses than in schizophrenia, which was confirmed by direct comparison with reference samples. Importantly, features related to language organization allowed machine learning classifiers to identify speech under LSD with accuracy comparable to that obtained by examining semantic content. These results constitute a quantitative and objective characterization of disorganized natural speech as a landmark feature of the psychedelic state.Fil: Pallavicini, Carla. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Fleni; Argentina.Fil: Sanz, Camila. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina.Fil: Carrillo, Facundo. Applied Artificial Intelligence Lab (ICC-CONICET); Argentina.Fil: Zamberlan, Federico. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina.Fil: Sigman, Mariano. Universidad Torcuato Di Tella; Argentina.Fil: Mota, Natalia. Federal University of Rio Grande do Norte. Brain Institute; Brasil.Fil: Copelli, Mauro. Federal University of Pernambuco. Fhysics Department; Brasil.Fil: Ribeiro, Sidarta. Federal University of Rio Grande do Norte. Brain Institute; Brasil.Fil: Nutt, David. Imperial College London. Department of Medicine. Centre for Psychedelic Research; Reino Unidos.Fil: Carhart-Harris, Robin. Imperial College London. Department of Medicine. Centre for Psychedelic Research; Reino Unidos.Fil: Tagliazucchi, Enzo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
Extracellular vesicles containing the transferrin receptor as nanocarriers of apotransferrin
Full text linkPrevious work by our group has shown the pro-differentiating effects of apotransferrin (aTf) on oligodendroglial cells in vivo and in vitro. Further studies showed the remyelinating effect of aTf in animal demyelination models such as hypoxia/ischemia, where the intranasal administration of human aTf provided brain neuroprotection and reduced white matter damage, neuronal loss, and astrogliosis in different brain regions. These data led us to search for a less invasive and controlled technique to deliver aTf to the CNS. To such end, we isolated extracellular vesicles (EVs) from human and mouse plasma and different neuron and glia conditioned media and characterized them based on their quality, quantity, identity, and structural integrity by western blot, dynamic light scattering, and scanning electron microscopy. All sources yielded highly pure vesicles whose size and structures were in keeping with previous literary evidence. Given that, remarkably, EVs from all sources analyzed contained Tf receptor 1 (TfR1) in their composition, we employed two passive cargo-loading strategies which rendered successful EV loading with aTf, specifically through binding to TfR1. These results unveil EVs as potential nanovehicles of aTf to be delivered into the CNS parenchyma, and pave the way for further studies into their possible clinical application in the treatment of demyelinating diseases.Fil: Correale, Jorge. Fleni. Departamento de Neurología. Servicio de Neuroinmunología y Enfermedades Desmielinizantes; Argentina.Fil: Mattera, Vanesa S. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Instituto de Química y Fisicoquímica Biológicas; Argentina.Fil: Pereyra Gerber, Pehuén. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica, Departamento de Química Biológica; ArgentinaFil: Glisoni, Romina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica, Departamento de Tecnología Farmacéutica; Argentina. Instituto de Nanobiotecnología (NANOBIOTEC), Universidad de Buenos Aires. CONICET, Buenos Aires, Argentina.Fil: Ostrowski, Matias. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica, Departamento de Química Biológica; Argentina.Fil: Verstraeten, Sandra V. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Instituto de Química y Fisicoquímica Biológicas; Argentina. Universidad de Buenos Aires. Instituto de Investigaciones Biomédicas en Retrovirus y SIDA; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Pasquini, Juana M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Instituto de Química y Fisicoquímica Biológicas; Argentina. Universidad de Buenos Aires. Instituto de Investigaciones Biomédicas en Retrovirus y SIDA; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Hemihypoglossal-Facial Nerve Transfer: Technical Description and Key Points: 2-Dimensional Operative Video
No full textIrreversible facial palsy, generally post-traumatic or postsurgical, can have devastating consequences for the patient from a functional, aesthetic, and psychological point of view. Among all of the reconstructive techniques, the hemihypoglossal-facial nerve transfer, which avoids the complete section of the hypoglossal nerve, is preferred by senior authors because of its excellent results and very low morbidity.1-5 This technique can be carried out in any neurosurgical center because it requires only basic instruments of microsurgery and a high-speed drill. However, detailed knowledge of the anatomy of the facial nerve in both its intrapetrosal and extracranial segments and of the hypoglossal nerve in its cervical segment is essential.1,6,7 Thus, previous practice in a cadaveric laboratory is recommended. The purpose of this video is to describe the technical nuances and key points of hemihypoglossal-facial nerve transfer. It was made using the surgical videos of 5 patients with a complete and irreversible facial paralysis who were operated using this technique in our institution between May and September 2019, all of whom consented to the procedure and to use for scientific purposes. The footages were edited, making a film in which the surgical technique is described in a stepwise fashion, emphasizing its most important features. To conclude, we would like to emphasize that the timing of surgery is of utmost importance and that this technique is both effective and reliable. Figures in the video at 00:54 and 01:35 are reprinted by permission from CCC: Springer Nature, Acta Neurochirurgica, Treatment of complete facial palsy in adults: comparative study between direct hemihypoglossal-facial neurorrhaphy, hemihipoglossal-facial neurorrhaphy with grafts, and massater to facial nerve transfer.Fil: Caffaratti, Guido. Fleni. Departamento de Neurocirugía; Argentina.Fil: Giovannini, Sebastián Juan María. Fleni. Departamento de Neurocirugía; Argentina.Fil: Orfila, Daniel. Fleni. Departamento de Neurocirugía; Argentina.Fil: Socolovsky, Mariano. Fleni. Departamento de Neurocirugía; Argentina.
Universidad de Buenos Aires. Hospital de Clínicas. Division of Neurosurgery. Nerve and Plexus Surgery Program; Argentina