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Mechanisms underlying hepatocyte recovery upon liver injury during growth spurt in zebrafish
No full textThe liver's regenerative capacity varies with injury severity. Minor injuries aretypically repaired by hepatocyte self-duplication, whereas severe damage involvescholangiocytes in hepatocyte recovery. While well-documented in adult animals, thisparadigm is less explored during rapid growth phases.This study introduces two liver injury models in zebrafish during growth spurts:partial ablation, killing half the hepatocytes, and partial hepatectomy, removing half aliver lobe. The novel CellCousin system, a genetic mosaic system, facilitates theinducible ablation of hepatocytes and subsequent lineage tracing of spared and denovo hepatocytes. In both injury contexts, de novo hepatocytes emerged alongsideexisting ones.Single-cell transcriptomics and lineage tracing with a Cre-driver line identifiedcholangiocytes as the source of these new hepatocytes. Additionally, active mTORC1signaling in the uninjured liver of growing animals was found to regulate cholangiocyteplasticity. This finding suggests cholangiocyte-to-hLa capacité de régénération du foie varie en fonction de la gravité de lablessure. Les blessures mineures sont généralement réparées par autoduplication deshépatocytes, tandis que les dommages sévères impliquent les cholangiocytes dans larégénération des hépatocytes. Bien documenté chez les animaux adultes, ceparadigme est moins exploré pendant les phases de croissance rapide.Cette étude présente deux modèles de blessure hépatique chez le poisson-zèbre pendant les poussées de croissance :l'ablation partielle, tuant la moitié deshépatocytes, et l'hépatectomie partielle, qui enlève la moitié d'un lobe hépatique. Lenouveau système CellCousin, un système génétique mosaïque, facilite l'ablationinductible des hépatocytes et le traçage de lignée des hépatocytes intacts et de novo.Dans les deux contextes de blessure, de nouveaux hépatocytes réapparaissent auxcôtés des hépatocytes existants.La transcriptomique unicellulaire et le traçage de lignée avec une lignée Cre-driver ont identifié les cholangiocytes comme sourDoctorat en Sciences biomédicales et pharmaceutiques (Médecine)info:eu-repo/semantics/nonPublishe
Architect-Editor Notes: Intermediate Evaluation Alice Paris 3/5
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Openbaar vervoer voor iedereen (vervolg)? Verplaatsingen binnen Brussel voor mensen met een handicap
No full textIn dit artikel onderzoeken we het verschil in toegang tot de stad voor mensen met een handicap wanneer ze het openbaar vervoer nemen in Brussel. Het onderzoek werd uitgevoerd op het niveau van de reisroutes vanaf alle haltes van de MIVB naar twintig geselecteerde bestemmingen in de stad. In onze berekeningen vergelijken we de onbeperkte toegankelijkheid met de toegankelijkheid die beperkt wordt door zes verschillende reisbeperkingen. De resultaten zijn zeer heterogeen, afhankelijk van de onderzochte reisbeperking, het vertrekpunt en de bestemming. Deze studie brengt grote verschillen qua toegang tot de stad met het openbaar vervoer aan het licht. Met reisbeperkingen nemen de meeste verplaatsingen namelijk meer tijd in beslag, moeten de reizigers vaker overstappen en moeten ze meer wandelen. Het feit dat het ondergrondse net niet inclusief is, heeft een aanzienlijke impact op de resultaten. Onze conclusie is dan ook dat er nog veel ruimte voor verbetering is om het openbaar vervoer in Brussel echt inclusief te maken.info:eu-repo/semantics/publishe
Dynamic transitions of initiator binding coordinate the replication of the two chromosomes in Vibrio cholerae
No full textAbstract The replication of the two chromosomes in the pathogenic bacterium Vibrio cholerae is coordinated by the binding of initiator protein RctB to a checkpoint sequence, crtS .Replication of crtS on the primary chromosome (Chr1) triggers replication of the secondary chromosome (Chr2), but the details are poorly understood. Here, we analyze RctB binding patterns in the V. cholerae genome across various cell cycle stages. We find that RctB primarily binds to sites inhibiting replication initiation at the Chr2 origin ( ori2 ). This inhibitory effect is counteracted when crtS is replicated on Chr1, causing a shift in RctB binding to sites that activate replication at ori2 .Structural analyzes indicate the formation of diverse oligomeric states of RctB, coupled to the allosteric effect of DNA, which determine ori2 accessibility. We propose a synchronization model where, upon replication, crtS locally destabilizes the RctB inhibition complex, releasing the Chr2 replication origin.SCOPUS: ar.jinfo:eu-repo/semantics/publishe
Recension du livre de JD Nasio (2024), Clara, le bébé qui revient à la vie. Psychologie clinique, Petite bibliothèque, Payot, Essais
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Prediction of energy resolution in the JUNO experiment BrowZine Journal Cover e, f; as; av; bn; bn;
No full textThis paper presents an energy resolution study of the JUNO experiment, incorporating the latest knowledge acquired during the detector construction phase. The determination of neutrino mass ordering in JUNO requires an exceptional energy resolution better than 3% at 1 MeV. To achieve this ambitious goal, significant efforts have been undertaken in the design and production of the key components of the JUNO detector. Various factors affecting the detection of inverse beta decay signals have an impact on the energy resolution, extending beyond the statistical fluctuations of the detected number of photons, such as the properties of the liquid scintillator, performance of photomultiplier tubes, and the energy reconstruction algorithm. To account for these effects, a full JUNO simulation and reconstruction approach is employed. This enables the modeling of all relevant effects and the evaluation of associated inputs to accurately estimate the energy resolution. The results of this study reveal an energy resolution of 2.95% at 1 MeV. Furthermore, this study assesses the contribution of major effects to the overall energy resolution budget. This analysis serves as a reference for interpreting future measurements of energy resolution during JUNO data collection. Moreover, it provides a guideline for comprehending the energy resolution characteristics of liquid scintillator-based detectors.0info:eu-repo/semantics/publishe
JUNO sensitivity to invisible decay modes of neutrons
No full textWe explore the decay of bound neutrons in the JUNO liquid scintillator detector into invisible particles (e.g. n→3ν or nn→2ν), which do not produce an observable signal. The invisible decay includes two decay modes: n→inv and nn→inv. The invisible decays of s-shell neutrons in 12C will leave a highly excited residual nucleus. Subsequently, some de-excitation modes of the excited residual nuclei can produce a time- and space-correlated triple coincidence signal in the JUNO detector. Based on a full Monte Carlo simulation informed with the latest available data, we estimate all backgrounds, including inverse beta decay events of the reactor antineutrino ν¯e, natural radioactivity, cosmogenic isotopes and neutral current interactions of atmospheric neutrinos. Pulse shape discrimination and multivariate analysis techniques are employed to further suppress backgrounds. With two years of exposure, JUNO is expected to give an order of magnitude improvement compared to the current best limits. After 10 years of data taking, the JUNO expected sensitivities at a 90% confidence level are τ/B(n→inv)>5.0×1031years and τ/B(nn→inv)>1.4×1032years.0info:eu-repo/semantics/publishe
Ligand exchange in TREN-based Cobalt (II) funnel complexes in the solid state
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