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Expanding the boundaries:regional connectivity in green turtle (Chelonia mydas) populations across Micronesia
Get PDFThe importance of ocean currents in influencing the connectivity and dispersal of marine megafauna, including sea turtles, is becoming increasingly recognized. However, more comprehensive studies are needed on how these currents impact the genetic makeup and connectivity of green turtle Chelonia mydas (Linnaeus, 1758) populations in the Pacific Islands region. To address this gap, our study conducted genetic analyses of green turtles in foraging grounds from four Micronesian sites and predictive modelling of hatchling dispersal patterns based on ocean currents to explore connectivity between foraging grounds and rookeries across Micronesia. We analyzed mitochondrial DNA (mtDNA) haplotype frequencies from newly collected and published data. We used Bayesian mixed stock analysis (MSA) to estimate the relative proportion of turtles from different Management Units (MUs) within the foraging grounds. Our ocean current simulations predicted hatchling dispersal trajectories, revealing how passive drift may facilitate wide-ranging dispersal and connectivity among distant foraging and nesting sites. Across the four Micronesian foraging grounds, the MSA revealed varying contributions from different rookeries, with some turtles exhibiting rare, long-distance dispersal traits. Our study highlights the intricate relationship between oceanic currents and the spatial dynamics of green turtles across the Pacific. Our findings offer essential insights into the conservation and management of green turtles by highlighting the role of oceanic currents in shaping population connectivity. Future research should focus on expanding genetic analyses and refining ocean drift simulations to enhance our understanding of marine megafauna migration and inform regional conservation strategies effectively.</p
Strengthening global plastic policy with systems analysis
No full textOver the last decades, plastic pollution has predominantly been considered a downstream issue, focusing on marine litter and waste management challenges. However, this limited focus is insufficient in responding to the urgency, complexity and scale of the problem. Therefore, the scientific community increasingly calls for a systemic focus on plastic pollution, especially with respect to designing effective policy interventions. Extant literature has documented examples of science-based policy, showing that systems tools have successfully informed policies targeting plastic pollution. With a potentially first, global plastics treaty currently under negotiations, we analyse the way in which systems-based techniques are used in literature to generate evidence regarding plastic pollution and to inform policy processes. We systematically select 324 publications from the systems-based plastic pollution literature and analyse trends, patterns and context in which systems analysis tools are used. We find that the application of these tools is limited and still highly focused on the downstream impacts of plastics. Thus, we make two key recommendations for harnessing systems analysis techniques to strengthen the efforts at developing an ambitious, science-based treaty: the employment of an emitter perspective and supporting a just transition through systems literature.</p
Principles for stimulating concurrent selective microbial growth and polyhydroxyalkanoate (PHA) storage responses for enhanced PHA productivity
Get PDFMunicipal wastewater treatment plants are a ubiquitous source of microbial biomass for PHA production. Technological feasibility of directly using municipal activated sludge (WAS) for a PHA accumulation bioprocess is demonstrated in the literature. However, PHA contents and yields may be lower due to a coexistence of PHA-storing and non-PHA-storing microorganisms in WAS. This work focused on metabolic principles for stimulating selective growth of PHA-storing microorganisms during a PHA accumulation bioprocess to enhance PHA productivity. Two model substrates, butyrate and acetate, were used to evaluate conditions and principles that may regulate this selective growth response. Conditions promoting selective growth of the PHA storers were consistently observed in the fed-batch PHA production process fed with butyrate. Productivity was increased to 4 times more PHA produced over 48 h, wherein PHA contents and average yields were improved from 0.39 gPHA/gVSS and 0.25 gCODPHA/gCOD to 0.61 gPHA/gVSS and 0.47 gCODPHA/gCOD, respectively. Respiration monitoring and mass balances, with metabolic modelling, suggest that expected underlying differences in ATP yields between two tested substrates are the main mechanistic drivers of the observed selective growth. This study proposed that if conditions are created such that ATP is produced in sufficient excess of the demands for PHA storage and cell maintenance, then those PHA storers will further grow concurrently. The selective microbial growth allows extra conversion of substrates into PHA. These principles, concerning the metabolic basis of the PHA production pathway, provide a foundation that can be applied to a range of substrates or substrate mixtures for enhanced PHA accumulation.</p
High-Dose Influenza Vaccine Effectiveness against Hospitalization in Older Adults
No full textBACKGROUND: High-dose inactivated influenza vaccine has been shown to provide protection against influenza that is superior to that with the standard dose. However, data from individually randomized trials on the effectiveness of the high-dose vaccine against severe outcomes are limited.METHODS: In this pragmatic, open-label, randomized, controlled trial conducted in Denmark during the 2022-2023, 2023-2024, and 2024-2025 influenza seasons, we assigned older adults (≥65 years of age) to receive the high dose of the inactivated influenza vaccine or the standard dose. Data collection relied on nationwide administrative health registries. The primary end point was hospitalization for influenza or pneumonia that occurred from 14 days after vaccination through May 31 of the following year.RESULTS: Of the 332,438 participants who underwent randomization, 166,218 were assigned to receive the high-dose vaccine and 166,220 to receive the standard-dose vaccine. The mean (±SD) age of the participants was 73.7±5.8 years, and 161,538 participants (48.6%) were women. A primary end-point event occurred in 1138 participants (0.68%) in the high-dose group and in 1210 (0.73%) in the standard-dose group (relative vaccine effectiveness, 5.9%; 95.2% confidence interval [CI], -2.1 to 13.4; P = 0.14). Hospitalization for influenza occurred in 0.06% of the participants in the high-dose group and in 0.11% of those in the standard-dose group (relative vaccine effectiveness, 43.6%; 95.2% CI, 27.5 to 56.3); hospitalization for pneumonia occurred in 0.63% and 0.63%, respectively (relative effectiveness, 0.5%; 95.2% CI, -8.6 to 8.8); hospitalization for cardiorespiratory disease in 2.25% and 2.38% (relative effectiveness, 5.7%; 95.2% CI, 1.4 to 9.9); hospitalization for any cause in 9.38% and 9.58% (relative effectiveness, 2.1%; 95.2% CI, -0.1 to 4.3), and death from any cause in 0.67% and 0.66% (relative effectiveness, -2.5%; 95.2% CI, -11.6 to 5.9). The incidence of serious adverse events was similar in the two groups.CONCLUSIONS: In this trial, a high-dose inactivated influenza vaccine did not result in a significantly lower incidence of hospitalization for influenza or pneumonia than a standard dose among older adults. (Funded by Sanofi; DANFLU-2 ClinicalTrials.gov number, NCT05517174; EU Clinical Trials Register number, 2022-500657-17-00.).</p
Author reply to comments by Wang et al. regarding "Musculoskeletal disease in acromegaly-a population-based registry study"
No full textVirtual Reality Music Training for CI Users:Insights and Design Guidelines
No full textCochlear Implant (CI) users face challenges with music perception, including pitch, timbre, and spatial sound localization. While music training programs (MTPs) exist on traditional platforms, the potential of Virtual Reality (VR) remains underexplored. This paper presents the iterative development and evaluation of MuCIcal Adventures, a VR-based music training prototype for CI users. Developed through a user-centered design approach over three iterations, the findings provide design guidelines for immersive, accessible, and effective VR applications tailored to this user group.</p
From Scale to Sustainability: Green Innovation, Media Attention, and Political Connection
No full textUrinary albumin-to-creatinine ratio in patients with hypertension and risk of major cardiovascular events
Get PDFIntroduction/aims Evaluation of urinary albumin-to-creatinine ratio (uACR) is a key component in the management of hypertension, yet there is a lack of data on the association between uACR and major cardiovascular events (MACEs) in large hypertensive cohorts, and it is also unknown how often uACR is measured among these patients. We aimed to evaluate the usage of uACR in a nationwide population of patients with hypertension. Furthermore, we sought to assess the risk of cardiorenal events according to uACR, among patients with hypertension. Methods We used Danish nationwide registries to identify patients who initiated antihypertensive treatment. The patients were grouped at treatment initiation according to uACR: normoalbuminuria, microalbuminuria, macroalbuminuria and no uACR measurement, and followed for 2 years, to evaluate the risk of a MACE, hospitalisation for heart failure (HF), 40% decline in estimated glomerular filtration rate (eGFR) and end-stage kidney disease (ESKD) according to uACR. Results We included 144 644 patients, of whom 116 039 (80%) did not have their uACR evaluated at treatment initiation. Patients with macroalbuminuria comprised the greatest 2 year absolute risk of MACE (5.3%, 95% CI: 4.0% to 6.6%) and had a greater risk of MACE (HR: 2.02, 95% CI: 1.54 to 2.66), HF (HR: 1.99, 95% CI: 1.35 to 2.95), 40% decline in eGFR (HR: 4.81, 95% CI: 3.78 to 6.10) and ESKD (HR: 4.52, 95% CI: 3.00 to 6.82) compared with patients with normoalbuminuria. Increased risk of MACE, HF and 40% decline in eGFR among patients with macroalbuminuria was persistent across subgroups of eGFR 120-30 mL/min/1.73 m². Conclusions In this real-world cohort, uACR was not regularly measured among patients initiating antihypertensive treatment. Nonetheless, the 2-year risks of cardiorenal events were considerably higher among patients with albuminuria compared with patients without.</p
Quality Assurance of a Hospital-Based Auditory Verbal Intervention for Children with Hearing Loss
Get PDFBackground: Auditory Verbal Therapy (AVT) has spread globally in the past few years due to its proven positive effect as a rehabilitation method for children with hearing loss (HL). In 2023, a three-year hospital-based AV intervention was implemented in Denmark as a government-funded offer to all children with HL. In the present study, we introduce and study a protocol for facilitating this implementation with a specific focus on clinical applicability and quality assurance. Methods: A working group was set up to drive the implementation process and establish a base for clinical collaboration and coproduction. The final protocol included (1) regular workshops and meetings with all AV specialists, (2) the creation of a database to collect data on the effect of the intervention, and (3) mandatory testing of the auditory skills and receptive vocabulary of the children with HL and a screening of their behavioural and emotional status with the Strengths and Difficulties Questionnaire (SDQ). Results: Data from 53 children with a mean age of 6.6 years were analysed in this study. Results from the SDQ showed that the children scored like the Danish norm on both the SDQ total difficulty score and SDQ prosocial score. Conclusions: This study’s findings indicate that children with HL, who participated in three years of hospital-based AV intervention, demonstrate the same emotional and behavioural problems and the same social strengths as their hearing peers. We hope that this study can inspire and guide others who want to implement an AV intervention in a hospital setting.</p
