SCTIMST DSpace (Sree Chitra Tirunal Institute for Medical Sciences and Technology)
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Dextran stabilized fullerene soot induced toxicity on alveolar epithelial cells (A549 cells)
No full textFullerene comprises the major allotrope of carbon holding several fruitful potentials to be applied in various industrial and biomedical scenarios. Scientists have acquired large number of data on fullerene research using its derivatives like C60, C70 etc. Nevertheless, a precise focus on fullerene soot nanopaticles and its toxic impacts in living tissue is still behind mainstay even if it represents the crude parent form of all other derivatives. Present study addresses an acute toxicity profiling of fullerene soot nanoparticles in alveolar epithelial cells (A549) as a paradigm of pulmonary exposure. Surface functionalization was given for fullerene soot nanoparticles using dextran polymer as a mean to establish a stable homogenous dispersion (denoted as dFSNPs hereafter). Following functionalization, dFSNPs were characterized for various parameters including size, surface charge, morphology and functional groups using DLS, Zeta potential analysis, TEM and FT-IR measurements respectively. Effective dextran functionalization was evident from the characteristic peaks in FTIR spectra. Cell viability assessed using MTT and NRU assays; both of which showed a dose dependent cytotoxic response. Thymidine incorporation also confirmed similar trend in viability rate. In accordance with literatures, DCFHDA assay confirmed free radical scavenging activity of fullerene nanoparticles. An altered cellular morphology was observed under fluorescent microscope. Sub-cellular functionalities including lysosomal integrity and mitochondrial stability were found to be compromised at highest tested concentration of dFSNPs (160 μg/ml) without any genotoxic impacts within nuclear premises. FACS analysis following Annexin-PI staining confirmed apoptotic cell death. Hence the overall study substantiated dose dependent toxicity of dFSNPs which is likely to occur during pulmonary exposure
In vitro and In vivo toxicity analysis of zinc selenium/zinc sulfide (ZnSe/ ZnS) quantum dots
No full textDespite the versatility of quantum dots (QDs) in optoelectronics and biomedical field, their toxicity risks remain a considerable hindrance for clinical applications. Cytotoxicity of Cadmium containing QDs is well documented and reveals that they are toxic to cells. Reports suggest that the presence of toxic elements at the QD core (e.g., cadmium, selenium) is responsible for its toxicity in in vivo and in vitro levels. Hence, here the toxicity of heavy metal free ZnSe/ZnS QDs on two scenarios were assessed, (i) HEK cells as in vitro system and (ii) Swiss Albino mice as in vivo model. Before toxicity analysis, QDs subjected to various optical and physico-chemical characterization methods such as absorption and emission spectra analysis, observation under U.V light, TEM, DLS, Zeta potential, FTIR, Raman and XPS spectra, ICP-OES, TGA and DTG curve. It is very necessary to characterize the synthesized QDs because their toxicity greatly influenced by the physico-chemical properties. On checking the vulnerability of HEK cells on exposure to ZnSe/ZnS QDs, the obtained results disclose that ZnSe/ZnS QDs showed merest impact on cellular viability at a concentration less than 100 μg/ml. Acute toxicity of 10 mg/kg ZnSe/ZnS QDs was studied in mice and no clinical or behavioural changes were observed. It did not induce any changes in haematological parameters and any loss of body or organ weight. Moderate pathological changes were evident only in the liver, all others organs like kidney, spleen and brain did not show any manifestations of toxicity. Current work lays substantial bedrock for safe biomedical and environmental application of ZnSe/ZnS QDs in near future
A study to assess the prevalence of undernutrition among children aged 3-5 in tribal area and the associated social determinants in Kendujhar district, Odisha
No full textComparitive study of motor unit number estimation in amyotrophic lateral sclerosis and post polio paralysis
No full textComparison of Outcomes following Conventional and Eversion carotid Endarterectomy A Retrospective study
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Clinical and radiological outcomes following foramen magnum decompression in patients with chiari malformation
No full textCognitive Outcome Following Bilateral Subthalamic Nucleus Deep Brain Stimulation in Parkinsons Disease: A Comparative Observational Study in the Indian Population
No full textEffect of polymer functionalized fullerene soot on C6 glial cells
No full textFullerene is crafting its dominance in various fields of science. Advanced exploration on the applicability of fullerene and the enhanced chances of exposure has prompted valuation of its biocompatibility. The antioxidant and radical scavenging characteristic of fullerene raises assurance for various biomedical applications. Nevertheless, the hydrophobicity of fullerene lowers its charm in healthcare applications where stable water dispersity is fundamental. In this study, one of the highly exposed forms of fullerenes; fullerene soot was subjected to in vitro toxicity evaluation. For nullify hydrophobic consistency, soot nanoparticles were surface functionalized with dextran polymer. Even though various tissue-specific toxicity evaluations of fullerene are been reported, the idea about its effect on the neuronal tissue still remains vague. In the context, the well-characterized dextran-coated fullerene soot (Dex-FS) nanoparticles were exposed to C6 glial cells to understand the cellular response of Dex-FS. Glial cells are present in plenty in the central nervous system (CNS) and provide support and insulation to neurons. Hence, glial cells turn out to be an ideal choice for in vitro CNS system. Dex-FS showed dose-dependent toxicity. The integrity of cytoskeleton was found to be compromised. Dex-FS was found to trigger ROS production in a dose and time dependent manner. The lysosomal activity and mitochondrial membrane potential during post Dex-FS treatment was evaluated using acridine orange and rhodamine-phalloidin staining. The molecular level toxicity was evaluated by DNA laddering and DAPI staining. The amount of live and dead cells after treating C6 glial cells with Dex-FS was estimated by Calcein AM/ PI flow cytometry. The neurotoxic impact of fullerene soot nanoparticles explored in the present study thus presents a debatable topic regarding vehicle exhaust which contains higher amounts of fullerene soot as active ingredient
