SCTIMST DSpace (Sree Chitra Tirunal Institute for Medical Sciences and Technology)
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Assessment of implementation and awareness of cigarettes and other tobacco products act(cotpa) in high schools and higher secondary schools of Kasaragod district, Kerala
No full textOutcome of total correction for isolated total anomalous pulmonary venous connection: a retrospective study
No full textNITRIC OXIDE-PRODRUG MEDIATED MOLECULAR AND BIOENERGETIC ALTERATIONS IN CARDIAC AND SKELETAL MYOBLASTS IN HYPERGLYCEMIA
No full textOptical coherence tomography based characterisation of culprit lesions in acute coronary syndrome
No full textDetermination of the bioavailability of zinc oxide nanoparticles using ICP-AES and associated toxicity
No full textAdvancement in nanotechnology has brought abundant number of products and materials in multiple fields including biomedicine owing to their unique physico-chemical properties. This further necessitates toxicity assessment of nanoparticles (NPs) before they are employed for product fabrication, medicinal, environmental or industrial purposes. Zinc oxide nanoparticles (ZnONPs) belong to the category of metal oxide NPs and hold quite a lot of possibilities to be applied in aforementioned scenarios. Present study addresses the probable outcomes of bio-nano interaction of ZnONPs with healthy adult Wistar rats. Sphere head shaped ZnONPs were synthesized via wet chemical method. Physico-chemical characterization was performed using number of sophisticated techniques including HR-TEM, Zeta potential analysis, TGA and XRD. Size of the particles was found to be 43 nm and ensured homogenous distribution with high purity. For in vivo studies, as synthesized NPs were administered into rats via intravenous (i.v.) and intraperitoneal (i.p.) routes. Animals were sacrificed on 3rd, 14th and 21st day of exposure. Metabolically relevant tissues like brain, liver, kidneys and spleen were isolated and analyzed for different parameters like gross pathology, haematology, neurotoxicity, target organ toxicity, immunotoxicity etc. Results suggests that ZnONPs did not elicit significant toxic responses in rat except a few anomalies with histology, ion content and antioxidant system within liver; thereby confirming potent hepatotoxicity. Hence the study recommends adopting surface functionalization strategies for reducing toxic response of ZnONPs during various application rationales
A preliminary study on monocyte-platelet interactions in diabetic subjects leading to phenotypic changes in monocytes
No full textBackground and objectives: Platelets become hyperactive during progression of inflammatory disease conditions such
as diabetes mellitus. We aimed to assess whether activated platelets (CD62+) interact with monocytes to form monocyteplatelet aggregates and such aggregates change the phenotype of monocytes into proinflammatory phenotype (CD14-CD16+) via a comparative
study between diabetic and non diabetic subjects. Methods: Based on inclusion and exclusion criteria, study subjects were chosen. Monocytes
isolated from buffy coat were characterised using immunostaining and flow cytometry. Phenotypic changes in monocytes were determined
through flow cytometry; interaction between monocytes and platelets were determined through ow cytometry, ESEM. Proteomic studies were
carried out to determine inflammatory marker expression. Results: Study revealed that there are monocyte-platelet interactions and alteration in
monocyte phenotypes occurring in diabetic subjects compared to control subjects. Western blotting analysis showed the expression of
inflammatory molecules IL-10 and MCP-1 in diabetic subjects, further confirming the proinflammatory nature of monocytes. Conclusion:
Platelet monocytes interaction in diabetic subjects may lead to the phenotypic alteration in monocytes. Inflammatory protein analysis and cell
surface marker analysis of monocytes may be considered as a preliminary tool for assessing complications during diabetes mellitus
Nano Fullerene Mixture Impose Threat to Alveolar Epithelial Cells
No full textFullerenes, the symmetric allotrope of carbon with bucky ball structure is gaining high magnitude of interest in material chemistry as well as in medical scheme. Fullerenes find its role in batteries, solar cells, for hydrogen storage, trapping reactive species, as lubricants, MRI contrast agents, protective coatings, superconductors and also in biomedical applications as neuroprotective agents, anti-oxidant, enzyme inhibitor, delivery vector, biosensor, for photodynamic therapy etc. Apart from the intentional exposure during usage in various applications, nano fullerene mixture is present in automobile/industrial exhaust, release from incomplete combustion of fossil fuels etc. Increased exposure entail the exigency for toxicity profiling of fullerene. Inhalation being a major route of nanoparticle exposure compels lungs at high risk of toxicity due to accumulation. Retention at the “alveoli site” potentially causes translocation into blood that further complicates the scenario. Improved perception on the effect of nano fullerene mixture on lung cells is needed to make a critical inference
Role of multiparametric MRI In post Transarterial Chemoembolisation response evaluation of Hepatocellular Carcinoma(HCC)
No full textHuman-Derived Scaffold Components and Stem Cells Creating Immunocompatible Dermal Tissue Ensuing Regulated Nonfibrotic Cellular Phenotypes
No full textRegeneration of large-sized acute and chronic wounds provoked by severe burns and diabetes is a major concern worldwide. The availability of immunocompatible matrix with a wide range of regenerative medical applications, more specifically, for nonhealing chronic wounds is an unmet clinical need. Extrapolating the in vitro tissue engineering knowledge for in vivo guided wound regeneration could be a meaningful approach. This study aimed to develop a completely human-derived and minimally immune-responsive scaffold comprising of acellular amniotic membrane (AM), fibrin (FIB) and hyaluronic acid (HA), termed AMFIBHA. The potential for in vivo guidance of skin regeneration was validated through in vitro dermal tissue assembly on the combination scaffold by growing human fibroblasts, differentiated from human adipose tissue-derived mesenchymal stem cells (hADMSCs). An effective method was standardized for obtaining decellularized amnion (dAM) for assuring better immuno-compatibility. The biochemical stability of dAM upon plasma sterilization (pdAM) confirms its suitability for both in vitro and in vivo tissue engineering. The problem of poor handling characteristics was solved by combining the dried dAM with fibrin derived from a clinically used fibrin sealant kit. An additional constituent HA, derived from human umbilical cord tissue, imparts the required water absorption and retention property for better cell migration and growth. Post sterilization, the combination scaffold AMFIBHA demonstrated hemo-/cytocompatibility, confirming the absence of detergent residuals. Upon long-term (20 days/40 days) culture of hADMSC-derived fibroblasts, the suppleness of generated tissue was established by demonstrating regulated deposition of collagen, elastin, and glycosaminoglycans using both qualitative and quantitative measurements. Regulated expressions of transforming growth factors-beta 1 (TGF-β1) & TGF-β3, alpha smooth muscle actin (α-SMA), fibrillin-1, collagen subtypes, and elastin suggest non-fibrotic fibroblast phenotype, which could be an effect of microenvironment endowed by the AM, FIB, and HA. In burn wound model experiments, immune response to cellular AM was prominent as compared to untreated/sham control wounds and decellularized AM-treated and AMFIBHA-treated wounds, ensuring biocompatibility. Wound regeneration with complete epithelialization, angiogenesis, development of rete pegs, and other skin appendages were clearly visualized in 28 days after treating large-sized (4 × 4 cm2), debrided, full-thickness third-degree burn wounds, indicating guided wound regeneration potential of AMFIBHA dermal substitute