Open Research Oklahoma (Oklahoma State Univ.)
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Rural college leaders program: Supporting rural student success through cohort-based leadership development
No full textThe Rural College Leadership Program (RCLP) report examines strategies for strengthening leadership capacity in rural community colleges across North Carolina. It emphasizes the importance of collaborative networks, equity-driven practices, and professional development to address unique challenges faced by rural institutions. Through case studies and faculty insights, the report highlights approaches for fostering student success, building sustainable partnerships, and leveraging local resources. The findings underscore the need for intentional leadership development to advance educational access and economic mobility in rural communities.falseEducational Foundations, Leadership and Aviatio
Exploring the effects of wingtip and linker substituents of the 2,6-dimethyl pyridyl- and 2,6-pyridyl-linked CNC ligands on the coordination chemistry of copper(i) and the solution-phase behavior of the CU-CNC complexes
No full textN-heterocyclic carbenes have recently been used as the supporting ligand of many transition metal complexes that have found their way as catalysts or co-catalysts in assisting various organic reactions. The NHC-containing structures range from centering around the carbene with large heterocycles connected to the backbone or wingtips to using a coordinating group like a pyridine ring linking two carbene units. Recently, we have reported Cu(I) complexes bound to the pyridine-linked bis-NHC ligands (ref 1). Our computational studies have shown a notable contribution from the pyridine moiety to these complexes' frontier orbitals of the Cu(I)-CNC unit. Here, we present the synthesis and characterization of a new class of Cu(I)-CNC complexes bearing para- and meta-substituted pyridine linkers, which appear as binuclear or trinuclear units in the solid state depending on the pyridine derivative. Also, we present the application of these complexes in air-assisted Sonogashira-type and Ullmann-type C-C coupling reactions
Tiny house
No full textI worked on a thermal energy storage system designed to work in a tiny house. I learned how to calibrate temperature sensors and flowmeters. I also learned how to calculate the uncertainty of the heat transfer rate of our measured heat transfer rate
From structure to function: Recombinant expression, purification and structural characterization of Ostrinia nubilalis pheromone binding protein 2
No full textEuropean corn borer (ECB) Ostrinia nubilalis (Onub), is a profoundly serious pest of corn, sweet corn, and other crops. ECB accounts for 5.5 to 8.5 million tons of corn
loss in an average year in Europe and other countries. ECB was introduced to United
States in the 1900s. This pest accounts for 20% of yield loss in North America, with
damages estimated to exceed US $1 billion annually. Pesticides and insecticides used to
control these pests cause harmful effects in the environment. Moreover, pests show
resistance to it. Thus, there is an urgent need for an alternative method for control of this
pest. Mating in lepidopteran moths, such as Ostrinia nubilalis is initiated with the
detection of female-secreted pheromones by the males. The pheromone binding proteins
(PBPs) present in the male moth antenna play a critical role in pheromone detection. Of
the five PBPs identified in Ostrinia nubilalis, OnubPBP2 and OnubPBP3 have male-biased expression. An alternate approach for pest control is to target the mating process
by intervening in the pheromone detection system of the male moth. A Structure-function
study of the OnubPBP2 is critical to interfere in the pheromone detection system of this
invasive insect pest in a species-specific and environmentally friendly manner. To
achieve this goal, we have cloned, expressed, purified, and investigated the recombinant
OnubPBP2 through biophysical characterization. We have determined the high-resolution
three-dimensional structure of OnubPBP2 by NMR spectroscopy
Use of machine learning techniques in reservoir and seal characterization to estimate carbon storage potential of the Alaska North Slope and Upper Miocene, onshore Louisiana, United States
No full textGeologic carbon storage is widely viewed as a critical component of the energy transition and is now receiving significant attention in the United States near legacy hydrocarbon fields. Louisiana and Alaska are two states that contain large amounts of petroleum infrastructure such as pipelines, roads, surface facilities, etc. that could be repurposed for CO₂ storage. This dissertation seeks to make initial carbon storage estimates for these areas at a site-specific and then a regional scale. To calculate initial carbon storage, chapter II explores how self-organized maps can be combined with post-stack inversion to better define high quality sands. The research highlights a project sized target within the middle Miocene containing 16, 24, and 52 megatons (P₁₀, P₅₀, P₉₀) of estimated CO₂ storage. Using self-organized maps and inversion attributes together is an effective way for investigating an area for carbon storage targets. In chapter III, the scope is expanded to the Greater Kuparuk Field on the Alaskan North Slope, where a pre-stack inversion enhances the interpretation and allows for geobody extractions that delineate sand trends. Improved mapping from seismic inversion and well log measurements provide a new Ivishak carbon storage estimate beneath the Kuparuk oilfield of 1.2, 1.8, and 3.8 (P₁₀, P₅₀, and P₉₀) gigatons. Finally in chapter IV, Shublik seal integrity is evaluated as a possible top seal for carbon storage. Elastic rock properties, seismic attributes, and shale-gouge analysis are combined to identify the faults that pose the highest risk for leakage. Results show the Shublik largely blankets the area, and seal integrity along with thickness is consistent except for the heavily faulted eastern area
Breed-specific responses to coccidiosis in chickens: Identification of intestinal bacteria correlated with disease resistance
No full textCoccidiosis, caused by obligate intestinal parasites of the genus Eimeria, is a significant enteric disease in poultry. Beyond its direct impact on animal health, production performance, and welfare, coccidiosis imposes a substantial economic burden, costing the global poultry industry up to $13 billion annually. Effective mitigation strategies for coccidiosis are still lacking, further complicated by restrictions on antimicrobial use and consumer demands for antibiotic-free production. Although different chicken breeds exhibit varying resistance to coccidiosis, no commensal bacteria have been directly linked to coccidiosis resistance. We hypothesize that coccidiosis-resistant chickens harbor unique commensal bacteria that confer colonization resistance against coccidiosis. To determine the relative resistance of different chicken breeds to coccidiosis, three chicken lines, including Fayoumi M5.1, Leghorn Ghs6, and Cobb broilers, were challenged with 50,000 sporulated Eimeria maxima (EM) oocysts or mock-infected on day 10. Evaluations of body weight changes, small intestinal lesions, and fecal shedding of oocysts were conducted on day 17. Ileal and cecal digesta were further collected from each chicken on day 17 and subjected to 16S rRNA sequencing for microbiome analysis. Our results indicated that Fayoumi M5.1 chickens are the most resistant to coccidiosis and intestinal dysbiosis compared to Cobb and Ghs6 chickens. Both the ileal and cecal microbiota were markedly different among the three chicken breeds under both healthy and coccidiosis conditions. Notably, various Lactobacillus, Ligilactobacillus, and Limosilactobacillus species were highly enriched in both the ileum and cecum of M5.1 chickens in response to coccidiosis, suggesting the potential of these lactic acid bacteria as probiotics for the mitigation of coccidiosis
Elucidating proton pumping in cyanobacteria Complex I using site-directed mutagenesis and phenotypic analysis
No full textComplex I is a protein complex classically found in the mitochondrial membrane of eukaryotes and is essential for generating ATP through oxidative phosphorylation and water by oxygen reduction. In bacteria such as cyanobacteria, the protein complex, known as the NDH-1 complex, is a part of the thylakoid membrane and drives the proton gradient to the lumen of the thylakoid membrane. This occurs through electron transfer from a ferredoxin (Fd) to a peripheral arm region where, through sequential reactions with iron-sulfur clusters, a plastoquinone is reduced to plastoquinol. This reduction process provides the energy to drive proton pumping by the membrane-spanning subunits of the complex. However, this coupling mechanism between electron transfer and proton pumping remains to be understood. Evidence has shown through using site-directed mutagenesis that conserved amino acid residues in transmembrane region subunits, in our case NdhF4 and NdhD4, are critical intermediates in proton transfer within the complex, protein pumping to the thylakoid lumen, and maintaining optimal cyclic electron flow (CEF). Additionally, the NdhO subunit in the peripheral arm region has become characterized as an inhibitor of cyclic electron flow by maintaining a strong affinity for ferredoxin after it has been oxidized through electron transfer. Our goal has been to further characterize these regions through mutagenesis studies and spectroscopic assays in order to gain a better understanding of the importance each one contributes to the coupling between electron flow and proton pumping. Notably, there has been insufficient evidence to support whether proton pumping is truly coupled with the exchange process of PQH2 and PQ in a proton-coupled electron transport mechanism. Future studies with the NdhB subunit, another transmembrane subunit that pumps protons, will help further characterize the mechanism this protein complex catalyzes and its importance in generating ATP and water. In conclusion, the NDH-1 complex is a highly intriguing protein complex that catalyzes a sophisticated reaction, and its study has real-world applications in mechanistic understanding and building a foundation for treating mitochondrial disease (Parkison’s, MS, etc.) that affects hundreds of thousands of people worldwide.Oklahoma State University. Niblack Research Scholars ProgramMicrobiology and Molecular Genetic
Home vegetable garden [superseded]
No full textThe Oklahoma Cooperative Extension Service periodically issues revisions to its publications. The most current edition is made available. For access to an earlier edition, if available for this title, please contact the Oklahoma State University Library Archives by email at [email protected] or by phone at 405-744-6311
Pond plants: Weeds or beneficial?
No full textThe Oklahoma Cooperative Extension Service periodically issues revisions to its publications. The most current edition is made available. For access to an earlier edition, if available for this title, please contact the Oklahoma State University Library Archives by email at [email protected] or by phone at 405-744-6311
Modified ACE-V methodology and error rate in bloodstain pattern analysis
No full textThe error rate of bloodstain pattern classification, and thereby the reliability of the discipline, has been a subject of consideration in American courts, especially since the 2009 publication of a report from the National Research Council, “Strengthening Forensic Science in the United States: A Path Forward”. While there is substantial research in the field of bloodstain pattern analysis, there have been relatively few published studies into the error rate of Bloodstain Pattern Analysis (BPA). Of the published studies, several critique the lack of an accepted methodology in BPA and none include a technical review step prior to evaluation of error, despite technical review being common practice amongst forensic scientists. This study examined the error rate of pattern classifications by participating bloodstain pattern analysts using a modified version of the ACE-V methodology. Further, the error rate was calculated before and after verification. The ACE-V (analysis, comparison, evaluation, verification) method is a methodology traditionally used by fingerprint examiners which has potential application in the bloodstain community as a methodology for analysis that mandates a technical review of results prior to issuance of reports. A modified version of the methodology (analysis, classification, evaluation, verification) was utilized for this study. Several error rates were calculated and compared, including the classically considered error rate (Type I error) which was determined to be 27.6% before verification and 21.1% after verification. This study confirmed that a verification step (or technical review step) served to reduce Type I classification errors, wherein analysts chose an incorrect classification and failed to include the correct classification. Furthermore, this study demonstrated the adaptability of the ACE-V methodology for use in bloodstain pattern analysis