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EnzymeMiner: automated mining of soluble enzymes with diverse structures, catalytic properties and stabilities
Get PDFMillions of protein sequences are being discovered at an incredible pace, representing an inexhaustible source of biocatalysts. Despite genomic databases growing exponentially, classical biochemical characterization techniques are time-demanding, cost-ineffective and low-throughput. Therefore, computational methods are being developed to explore the unmapped sequence space efficiently. Selection of putative enzymes for biochemical characterization based on rational and robust analysis of all available sequences remains an unsolved problem. To address this challenge, we have developed EnzymeMiner-a web server for automated screening and annotation of diverse family members that enables selection of hits for wet-lab experiments. EnzymeMiner prioritizes sequences that are more likely to preserve the catalytic activity and are heterologously expressible in a soluble form in Escherichia coli. The solubility prediction employs the in-house SoluProt predictor developed using machine learning. EnzymeMiner reduces the time devoted to data gathering, multi-step analysis, sequence prioritization and selection from days to hours. The successful use case for the haloalkane dehalogenase family is described in a comprehensive tutorial available on the EnzymeMiner web page
Povstanie PKK/KCK v rokoch 2015-2016 v turecku z hôr do mesta a naspäť : Priebeh a poučenia z bojov
Get PDFText detailne mapuje priebeh povstania PKK/KCK v Turecku v rokoch 2015-2016. Autor okrem samotného popisu vývoja povstania čitateľovi predkladá detailný obraz bojov v urbánnych oblastiach tureckého juhovýchodu, ktorý predstavoval bezprecedentnú novinku v rámci vyše 30 rokov trvajúceho konfliktu medzi Kurdskou stranou pracujúcich a Tureckom. Zámerom je identifikovať kľúčové charakteristiky a taktické prvky využívané ako PKK/KCK, tak tureckými ozbrojenými zložkami a zároveň vyvodiť možné závery vyplývajúce z tureckých skúseností vedenia ozbrojeného boja v urbánnych oblastiach.The text focuses on the PKK/KCK insurgency in Turkey in 2015-2016. Besides the description of the course of the conflict, the article provides a detailed picture of the urban warfare in southeast Turkey during the selected period. The urban guerrilla campaign of the PKK/KCK was a novelty in the 30-year conflict between the insurgent organization on one side and the Turkish state on the other. The aim is to identify key characteristics and tactical features used by the PKK/KCK and the Turkish security forces respectively. The conclusion of the text brings an analysis of the conflict together with recommendations for urban warfare and the lessons Turkey learned in 2015-2016
Czech Farmers' Experience with Bt Maize : Fulfilment, and the Opposite, of Monsanto's Promises
Get PDFGenetically modified (GM) Bt maize MON810 is promoted as being beneficial for farmers and the environment. The aim of this study was to compare particular claims of the benefits associated with this GM maize with Czech farmers' practice. Ten semi-structured interviews and 27 questionnaires obtained from Czech farmers who had grown Bt maize for at least one year in 2005–2016 were analysed qualitatively. The alleged benefits of MON810 cultivation regarding lower levels of fungal disease infestation, yield increase, reduction of insecticide usage, lowered costs, time saving and simple manipulation were enjoyed by a varying proportion of Czech GM farmers. In contrast, some farmers experienced the opposite of the claimed benefit regarding yield, costs, manipulation and time requirements. The exception was the 100% control of the European Corn Borer (ECB). The current and previous studies suggest that most of the benefits manifest themselves rather on farms under high ECB pressure. Seen from an economic viewpoint, the cultivation of Bt maize could be recommended in areas with persistent high corn borer pressure. However, the benefit to the environment is challenged and it is suggested that farmers should employ a complex of cultural control methods
Ku koreňom dnešných dezinformačných kampaní. Aktívne opatrenia rozviedky komunistického Československa
Get PDFVäčšina akademických prác o dezinformáciách skúma súčasnú podobu tohto nebezpečného javu. Predkladaná štúdia sa na tento jav zamerala štúdiom archívnych dokumentov a je venovaná jednej z hlavných pracovných metód rozviedky komunistického Československa, a jej implementácii tzv. - aktívnym opatreniam. Text sa zameriaval predovšetkým na konkrétne, zatiaľ nepublikované, aktívne opatrenia, zamerané najmä na operácie uskutočňované v spolupráci medzi bývalými spravodajskými službami Východnéhobloku. Prezentované sú hlavne operácie, ktorých cieľom bol „hlavný nepriateľ“, USA a NATO, a operácie proti ich spojencom. Štúdia vychádza z faktu zjavnej personálnej kontinuity ruských tajných služieb teda aj nutnej kontinuity myslenia, prístupu k spravodajskej činnosti. Výsledky potvrdili tento predpoklad a preukázali skutočnú kontinuitu fenoménu dezinformácií, ktorý dnes využíva nové technológie v kombinácii s predchádzajúcimi prístupmi.Most academic papers on the disinformation examine the current form of that dangerous phenomenon. The presented study focused on this phenomenon through the study of archival documents and was devoted to one of the main work methods of 1.st Administration of the National Security Corps, which was the Intelligence Agency of the Communist Czechoslovakia, and its implementation of the so-called active measures. The text focused mostly on concrete, so far unpublished, active measures, focusing in particular on operations carried out in the interest of cooperation between the former services of the former Eastern Bloc. Presented were mainly operations whose target was the "main enemy", the United States and NATO, and the operations against its allies.The study was based on the assumption that, with the apparent personnel continuity within the Russian secret services, there was a continuity of thinking, and thus also a continuity of intelligence activity approaches.The results confirmed this assumption and demonstrated the actual continuity of disinformation phenomenon, which only uses new technologies today, yet combined with former approaches.Therefore, the use of available archival documents should not be ignored in nowadays recognition and combat of the phenomenon of misinformation. Comparative archival approach can be used today in education and information to improve safety
TAKING A SNAPSHOT : Discovering Escalation Prone Countries Through Structural Conditions and QCA
Get PDFThis paper introduces a method of conflict escalation prediction based on long-term structural conditions, drawn from open sources, and QCA (Qualitative Comparative analysis). The combination of the two components offers a model of prediction, which is comparable to other more sophisticated and resourced systems. Moreover, it offers a more sensitive estimation of an escalation.Tento článek představuje metodu předvídání eskalace konfliktů na základě dlouhodobých, strukturálních podmínek z otevřených zdrojů za použití kvalitativní komparativní analýzy (QCA). Kombinací těchto dvou prvků vzniká model a metoda predikce, která je v efektu predikce srovnatelná s dalšími, daleko sofistikovanějšími a lépe financovanými systémy a nabízí citlivější odhad eskalace
(Ne)využívání civilní odbornosti příslušníků aktivní zálohy : Nevyužitá příležitost personálních kvalit
Get PDFText předkládá výsledky výzkumuzaměřenéhona využívání civilní odbornosti příslušníků Aktivní zálohy ze strany Armády České republiky. Vrámci řešení výzkumu bylo zjištěno, že až na malou skupinu úzce specializovaných jednotek Aktivní zálohy nedochází ani khlubšímu zjišťování civilní odbornosti uvlastních členů ani uchazečů.Text sums up outcomes of researcher focused on utilizing civilian expertise during their service in Czech Army Active Reserve Forces. The main outcome is that Czech Army is not aware of individual civilian expertise of its reservist and except small group of highly specialized units the armed forces are noteven interested in this matter
Les funcions pragmatiques del marcador discursiu eh? en les interaccions parlamentaries
Get PDFL’article pretén discernir les funcions pragmatiques del marcador discursiu eh? en el llenguatge parlamentari catala actual. Els marcadors discursius, en general, ajuden a guiar les interpretacions dels enunciats emesos, es tracta d’eines de la interacció social. En aquest treball analitzarem l’ús del marcador eh? en els Diaris de Sessions i veurem si el marcador en qüestió representa un recurs eficaç per a l’argumentació típica d’aquesta mena de discurs, veurem quina és la seva productivitat en les diferents classes d’actes de parla i quina estrategia pragmatica s’associa fonamentalment a cada classe d’acte de parla.The work tries to find out the pragmatic functions of the discourse marker eh? in the parliamentary language of the actual Catalan. The discourse markers, generally, help to guide interpretations of the statements uttered, the issue is that they are means of the social interaction. In this work we analyse the use of the marker eh? in the Diaris de Sessions. We will see if the mentioned marker represents an efficacious resource for the argumentation typical of this kind of discourse, we will see which is his productivity in different groups of the speech acts and which pragmatic strategy does mainly connect with each groups of the speech acts
Muži Slovácké brigády v bojích na Slovensku
Get PDFČlánek se věnuje formování jednotek Slovácké brigády a jejich účasti na bojích o Slovensko v letech 1918-1919.The paper deals with creation of the Slovak Brigade troops and their participation on fights in Slovakia between years 1918 and 1919
Výjimky a omezení práva autorského: § 38g-39
No full textKapitola interpretuje paragrafy 38g a 39 autorského zákona, tj. licenci pro karikaturu a parodii a licenci pro osoby se zdravotním postižením.The chapters provide for interpretation of secs. 38g and 39 of the Copyright act, i.e. Licence for Caricature and Parody and Licence for Disabled Persons
Zaměstnanecké dílo: § 58
No full textKapitola interpretuje paragraf 58 autorského zákona, tj. zaměstnanecké dílo.The chapter provides for interpretation of sec. 58 of the Copyright Act, i.e. work for hire