398 research outputs found
Sort by
Measurements of broad sense heritability of generation time and motility in cancer cell lines
Get PDFExperiments were conducted over 7 months to record the motility and generation time of four cancer cell lines - HeLa, HT1080, MCF7 and MDA-MB-231 - in cell culture. Cell lines were cultured in vitro on a well plate for 24 hours before being placed into the environmental chamber of a Nikon TiE Timelapse Microscope. Multiple points within every well were chosen and videoed over a period of 72 hours. These videos were then analysed and cell motility and division tracked using MtrackJ and ImageJ. Records were kept of which cells divided and the track number assigned to each daughter cell. This allowed comparison between and within cell families so the heritability of the cell traits, motility and generation time, could be estimated. Broad sense heritability was estimated as the slope parameter of an ordinary least squares regression between related cells. Three cell-cell relationships were tested, mother-daughter, sister-sister and cousin-cousin. Our results showed that cell motility has high and significant heritability for all cell lines tested whereas generation time showed little or no significant heritability for the cell lines tested. This is consistent with expectations that traits closely linked to fitness will in general have lower heritability. These results are important for cancer evolution as heritability (a prerequisite for natural selection to be able to act) has never before been measured for any traits in cancer cells
Ash dosage and concentration risk dataset
No full textThis dataset was generated at the University of Reading (UK) and was funded by NERC under the Environmental Risks to Infrastructure Innovation Programme. The dataset provides data sufficient to plot Figures 2, 3, 4, 6 and 7 presented in Prata et al. (2018). The dataset includes NAME dispersion model output for a control run of a hypothetical Katla eruption, processed data that represent model agreement and risk maps, waypoints produced using flightCode for an air-route from New York (JFK) to London (LHR) return and processed data that represent ash dosage, concentration, model agreement and risk calculated along an air-route from New York (JFK) to London (LHR) return
Yield parameter relationships of oilseed rape (Brassica napus) under the influence of fertiliser and insect pollination
Get PDFThis dataset is made up of data from three studies. The first involves a flight cage trial where oilseed rape plants were grown under four fertiliser treatments and then exposed to insect pollination or not. A number of yield parameters were then collected from each plant. The second study involved a field study across three oilseed rape fields with insect pollinator exclusion cages placed over areas of the crop. Yield parameters were then collected from plants under these exclusion cages as well as open controls. The final dataset includes field data collected from a number of oilseed rape study plots where plant yield, seeds per pod, pod number and thousand grain weight has been established from a number of individual plants across these plots
SISAL (Speleothem Isotopes Synthesis and AnaLysis Working Group) database Version 1.0
No full textNote: This edition of the SISAL database was withdrawn on 13 July 2020. It has been superseded by a new edition available at http://dx.doi.org/10.17864/1947.256.
Stable isotope records from speleothems provide information on past climate changes, most particularly information that can be used to reconstruct past changes in precipitation and atmospheric circulation. SISAL (Speleothem Isotope Synthesis and Analysis) is an international working group of the Past Global Changes (PAGES) project. The working group aims to provide a comprehensive compilation of speleothem isotope records for climate reconstruction and model evaluation. The first version of the SISAL database contains oxygen and carbon isotope measurements from 371 individual speleothem records, and 10 composites from 174 cave systems worldwide, and metadata describing the cave settings and age models of these records
30 minute averaged overview data from the Silsoe Refresh Cube Campaign (RCC)
Get PDFAll 30 minute averaged data taken during the Refresh Cube Campaign (RCC) at Silsoe using the 6 m^3 test structure at the site and eight other 6 m^3 straw cubes undertaken as part of the PhD work of Gough (2017) and forms the full-scale experiments of the REFRESH project.
The data-set is split into two sections: an isolated cube and the array case with three different opening set-ups being undertaken for both array and isolated.
The array was in place October 2014 to April 2015, and the cube was isolated from May 2015 to July 2015.
Details of the experimental set-ups are available in publications.
The data contained within this document are 30 minute averaged and quality controlled using code previously used for the ACTUAL project.
The data set contains wind speeds, wind directions, internal and external temperatures, surface pressures, CO_2 concentrations and ventilation rates calculated from the pressure difference methods. Internal and external measurements are included for the flow
Questionnaire survey results of respondents in four office buildings for post-occupancy evaluation
Get PDFThis dataset records the questionnaire survey results of post-occupancy evaluations conducted with occupants of four office buildings in the United Kingdom according to their subjective sensations. For each building, three groups of main influential factors (Indoor Environment Quality, Occupants' Performance and Workplace Quality) were surveyed and a separate data file was created. The data can be used for building post-occupancy evaluation based on an analysis method such as the Analytic Hierarchy Process
Supplementary data for "First observations of gaseous volcanic plume electrification: a new source of charge for volcanic lightning"
No full textThis dataset contains the processed radiosonde and additional surface VOLCLAB sensor data for a measurement campaign at Stromboli volcano during 28th September to 4th October 2017.
Full details are given in the paper titled "First observations of gaseous volcanic plume electrification: a new source of charge for volcanic lightning", submitted to Nature Geoscience. The data for figures 1,3 and 4 are provided here
Simulations with the sea ice model CICE documenting the impact of improved sea ice physics
Get PDFSea ice thickness data (CryoSat-2) have been used to identify and correct shortcomings in simulating winter ice growth in the widely used sea ice model CICE. Here, we provide the data from CICE simulations documenting the impact of improved sea ice physics and the sensitivity to forcing and initial data. Adding a model of snow drift and using a different scheme for calculating the ice conductivity improve model results. Sensitivity studies
demonstrate that atmospheric winter conditions have little impact on winter ice growth, and the fate of Arctic summer sea ice is largely controlled by atmospheric conditions during the melting season. A full description of the data processing and uncertainties is given by
Schröder et al. (2018), 'New insight from CryoSat-2 sea ice thickness for sea ice modelling', in The Cryosphere. All simulations are listed in Tables 1 to 3 of this paper
Raw and processed neutron reflectometry and infrared spectroscopy data for oxidation of galactocerebroside monolayers
No full text1 - Raw neutron reflectometry event mode data
2 - Reduced reflectivity curves for 20 second slices of neutron reflectivity data
3 - Fitted neutron reflectometry time series plots
4 - Raw infrared spectra and spectra series
5 - Integrated peak areas calculated from IR spectra
6 - Time series plots of IR peak data
7 - Various code used to process the abov
The impact of precipitating hydrometeors on the atmospheric radiation budget
No full textThis dataset contains atmospheric radiative fluxes and heating rates. These fluxes and heating rates were calculated in order to quantify the effect of precipitating hydrometeors (particularly rain) on the radiation budget of the atmosphere and thereby determine whether climate and weather models should include a treatment of the radiative effect of rain. The dataset includes 4 files. all_hydro.nc and no_rain.nc contain full resolution SW and LW radiative fluxes at the surface and top of atmosphere for calculations that include all hydrometeors and all hydrometeors except rain, respectively. hydro_rad_effects.nc includes results from additional calculations at a lower resolution. case_study_data.nc includes full vertical profiles of radiative fluxes and heating rates at full resolution for 3 points that were chosen as case studies