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Generation of Pax5-deficient DT40 mutant cells, Pax5(-), and protein and mRNA levels of IgM H- and L-chains artificially and excessively accumulated in Pax5(-) are rapidly reduced via a lot of generations during continuous cultivation
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IgM H- and L-chains artificially and excessively accumulated in HDAC2(-/-) DT40 mutants are gradually and dramatically reduced in distinct manners in individual mutant clones via a lot of generations during continuous cultivation
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Fundamental and distinct manners for irreversible creation of chromatin structure plasticity of proximal 5'-upstream regions of particular transcription factor genes with epigenetic modifications for gain of new cell function to exclude accumulated IgM H- and L-chains in individual clones of HDAC2(-/-) DT40 mutants via a lot of generations during continuous cultivation
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