RepoMed (Medizinische Hochschule Hannover)
Not a member yet
3024 research outputs found
Sort by
Analyse der gesundheitsbezogenen Lebensqualität von Patienten mit spinaler Muskelatrophie (SMA) unter der Therapie mit Nusinersen und Ermittlung der Belastung ihrer pflegenden Angehörigen
Full text linkReduced exercise capacity in pediatric post-COVID syndrome correlates with time post infection and does not affect quality of life
Full text linkObjectives: Post-COVID syndrome (PCS) in children and adolescents is reported less frequently and typically of shorter duration than in adults but can be associated with significant morbidity and reduction of quality of life (QoL). In pediatric PCS, data on exercise capacity (EC) are rare. Methods: This prospective, monocentric, cross-sectional study, analyzed EC, QoL, and clinical parameters in pediatric patients with PCS (n = 29/53 girls, 14.4 ± 2.5 years). A total of 210.0 ± 104.2 days passed between SARS-CoV-2 infection and study-related examinations. Results: The main PCS symptoms were reduced subjective EC (92.4%), shortness of breath (64.2%), concentration difficulties (60.4%), and breathlessness (47.2%). In patients with PCS, EC was 76.6 ± 16.0% VO2peakpredicted and maximum workload 76.0 ± 17.9% norm. Overall QoL (Kindl-R total score) was 89.2 ± 17.3% norm, self-assessed physical wellbeing was 60.7 ± 30.4% norm, and emotional wellbeing was 85.1 ± 23.2% norm. We grouped the PCS patients into deconditioned vs. conditioned (threshold 80% of VO2peakpredicted EC). No group differences in age, bodyweight, height, muscle mass, fat percentage, BMI, lung function, neuropsychological symptoms, and wellbeing were found. While maximum workloads and VO2peak differed significantly according to grouping, lactate levels and self-assessed exertion were comparable. However, elapsed time after SARS-COV-2 infection was significantly shorter in deconditioned vs. conditioned patients (mean 198.5 ± 120.7 vs. 230.8 ± 62.6 days; p = 0.021). Conclusion: Pediatric PCS is associated with reduced EC, which is significantly impacted by time post SARS-CoV-2 infection, but does not appear to affect the QoL or self-esteem of the patients in this study
Stroke in patients with left ventricular assist device (LVAD): who is at risk?: a retrospective observational study at a tertiary care center
Full text linkObjectives: Stroke is a severe complication in patients with left ventricular assist devices (LVAD), significantly affecting quality of life and potentially leading to death. This study aimed to illustrate the clinical features, outcomes, and risk factors associated with stroke in LVAD patients, with the goal of identifying potential treatment targets. Methods: In a study of 249 consecutive patientswho underwent LVAD implantation, detailed evaluations were conducted regarding clinical characteristics, perioperative management, cardiovascular risk factors, comorbidities, and brain imaging. The etiology, treatment, and outcomes were subsequently assessed in individuals who encountered a stroke. Results: Eighty-three cerebrovascular events (CVE) occurred in 54/249 patients during a median study period of 2.2 years (0.4–3.5) with 53 ischemic events and 22 intracranial hemorrhages (ICH). Early peri- or postoperatively CVE in context to the LVAD implantation were identified in 31 patients. Competing risks regression analysis revealed that postoperative dialysis was associated with higher risk for CVE, considering death as competing risk event (HR 3.617; 95%-CI: 1.78–7.35; p 0.001). Modified Rankin Scale at outpatient visit did not differ in early CVE [3 (IQR 2–5) vs. 3 (IQR2–4), p = 0.146]. Late CVE frequently occurred during hospitalization for sepsis or in cardiac rehabilitation [n = 16/41 events (39%)]. Competing risk analysis treating death and heart transplantation as competitors identified history of stroke as associated factor [HR 3.564; 95%-CI (1.67–7.169); p = 0.001]. Mortality was not associated with CVE [with n = 27/54 (50%) vs. without CVE 94/195 (48.2%) p = 0.183]. TYPE Original Research PUBLISHED 09 September 2025 DOI 10.3389/fcvm.2025.1591208 Frontier
Comparative pilot study of three commercial kits for bacterial DNA extraction from human subgingival biofilm samples collected with a single paper point
Full text linkObjective: In periodontal research, subgingival biofilm samples are typically collected using sterile paper points and pooled for molecular analyses. Streamlining this process by using a single paper point for molecular analysis could simplify sample collection and allow additional paper points to be used for other investigations. This pilot study evaluated the performance of three commercial DNA extraction kits for analysing small sample volumes (<10 μL). Methods: Samples were collected from six participants, each contributing 18 paper points from both healthy and periodontitis-affected sites. Bacterial and human DNA yields were quantified using fluorometric measurements combined with qPCR, employing universal 16S primers for bacterial DNA and human-specific GAPDH primers. Results: Among the tested kits, the DNeasy Blood and Tissue Kit demonstrated the highest efficiency, yielding significantly more total dsDNA in samples from healthy sites compared to both other kits and in samples from periodontitis-affected sites compared to one kit. Bacterial DNA yields were also significantly higher with the DNeasy Kit compared to one of the other kits in both health conditions. Conclusion: These results suggest that one paper point is sufficient to extract DNA for subsequent bacterial analyses and that the DNeasy Blood and Tissue Kit appears to be the most efficient among the three tested kits
Low triglyceride levels are associated with increased risk of immune-related adverse events in patients receiving immune checkpoint inhibitors
Full text linkImmune checkpoint inhibitors (ICI) have revolutionized cancer therapy by enhancing anti-tumor immune responses, yet their use can lead to immune-related adverse events (irAE), including neurological complications. Despite their clinical relevance, predictive biomarkers for irAE remain scarce, and early identification of at-risk patients is a major unmet need. In this prospective study, 200 patients undergoing ICI therapy were enrolled, of whom 59 underwent longitudinal metabolomic profiling at baseline, three months, and six months. Thirty-two patients who developed irAE were compared to 27 age- and sex-matched individuals without irAE. Multivariate analyses, including Principal Component Analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS-DA), revealed distinct metabolomic signatures differentiating the two groups. Notably, baseline levels of triglyceride 20:0_34:1 were significantly lower in irAE(+) patients. In female patients, additional triglyceride species-20:1_34:2, 20:2_34:2, and 20:2_34:3-were also reduced prior to therapy and showed increases within three months of ICI initiation. These findings suggest that specific triglyceride species may serve as early biomarkers for irAE risk, particularly in female patients. The observed dynamic changes point to a potential link between lipid metabolism and immune-related toxicity, supporting the integration of metabolomic profiling into future strategies for risk stratification and personalized monitoring in cancer immunotherapy
MetaboSER -a platform for selecting, exchanging, and visualizing metabolomics data with controlled data access
Full text linkBackground The growing number of metabolomics studies, based on high-dimensional data measured by hyphenated mass spectrometry (MS) and/or nuclear magnetic resonance (NMR) spectroscopy, has sparked the creation of several public metabolomics data repositories. Each repository emphasizes different aspects regarding data selection and representation, but most offer only limited options for privacy-preserving data sharing. Results We present MetaboSERV, an open-source, browser-based metabolomics platform dedicated to the selection, integration, and sharing of quantitative metabolomics data and metadata with controlled data access. MetaboSERV aims to aid researchers in analyzing their results by facilitating means to browse, visualize, and compare data across available datasets. It provides different access control functionalities, creating an environment in which data can be shared safely in a privacy-preserving manner to support collaborative and interdisciplinary research. Furthermore, it is designed to be extensible and adaptable to existing data management infrastructures through the creation of self-managed MetaboSERV instances, for which we provide the source code and a set of configurable Docker images. Conclusions The public MetaboSERV instance is available at https://metaboserv.ckdn.app, and the source code can be found at https://gitlab.gwdg.de/MedBioinf/metabolomics/metaboserv. The Research Resource Identifier (RRID) for MetaboSERV is SCR_025496
Stationsschließungen bei nosokomialen Ausbrüchen in der Pädiatrie und Neonatologie: Ergebnisse eines systematischen Reviews
Full text link
Mechanisms of natural killer cell-mediated clearance of senescent renal tubular epithelial cells
Full text linkIntroduction: Intact kidney function is essential for fluid and electrolyte homeostasis, with renal tubular epithelial cells providing critical functions in the reabsorption or secretion of numerous metabolites. Cellular senescence of the renal epithelial cells can lead to functional deficits. Therefore, immune-cellmediated clearance of senescent epithelial cells can be an important factor for the maintenance of kidney function. Methods: In this study, we established a model to directly monitor natural killer (NK) cell-mediated clearance of senescent renal tubular cells. To this end we used primary cell co-cultures with different read-outs and life cell imaging. Results and Discussion: We observed that the clustering of NK cells on senescent renal epithelial cells could be used to detect senescence-triggered NK cell activation. Also, we found that NKG2D signaling and perforin-dependent lysis of senescent renal epithelial cells were crucial steps in the lysis of senescent cells. In addition, NK cell-mediated attack of senescent renal epithelial cells could be dampened by the addition of cyclosporine A and was augmented by the addition of interleukin 7. Together, these data show that NK cells can efficiently mediate the clearance of senescent renal tubular epithelial cells involving NK cell activation by NKG2D signaling
Increased epicardial tissue and reduced TAPSE and MAPSE scores in borderline personality disorders. Early indicators for cardiovascular risk?
Full text linkIntroduction Life expectancy of patients with borderline personality disorder (BPD) is reduced compared to the general population, which has been in part attributed to a heightened risk for cardiometabolic disorders. One prior study reported increased values of epicardial adipose tissue (EAT), which has been shown to be a sensitive marker for cardiovascular disease risk. Against this background, studies assessing cardiac function in patients with BPD have been missing to date. Methods The present study included 28 female patients with a diagnosis of BPD and 28 age, sex, and BMI-matched controls (mean age 29 ± 11 years). EAT thickness and parameters of cardiac function were assessed by echocardiography. Diabetes risk was assessed using the Finnish Diabetes Risk (FINDRISC) score, and metabolic syndrome was defined in accordance to National Cholesterol Education Program Adult Treatment Panel-III (NCEP/ATPIII) criteria. Additionally, self-report questionnaires were used to assess lifestyle factors, retrospectively reported childhood trauma and current symptoms of depression and anxiety. Results Our study confirmed significantly elevated levels of EAT in patients with BPD compared to controls. Additionally, significant decreases in right (TAPSE) and left (MAPSE) ventricular function, albeit within the normal range, were measured in BPD patients. Contrarily, left ventricular ejection fraction was similar in both groups. Further, patients with BPD reported high levels of childhood trauma and clinically relevant depression and anxiety symptoms. Diabetes risk and frequency of metabolic syndrome as well as serum levels of prognostic markers NT-proBNP and GDF15 were similar in both groups. BPD patients were more frequently smokers and reported lower levels of physical exercise compared to controls. Conclusion The present study demonstrates morphological and functional differences in a matched sample of female patients with BPD and healthy controls, pointing to an increased risk for the development of cardiovascular disorders. These findings highlight the importance of screening for cardiovascular risk markers and of including interventions that aim to improve adverse life-style habits early on in the clinical management of BPD