RepoMed (Medizinische Hochschule Hannover)
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Elektrisch betriebenes Stapler-System (iDrive) versus mechanischer Klammernahthandgriff in der minimal-invasiven Lungenchirurgie: Ergebnisse einer prospektiv randomisierten Studie
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Postoperative Zufriedenheit bei Patienten mit elektiven Operationen an der Wirbelsäule in Abhängigkeit von Persönlichkeitsstruktur und psychischen Faktoren
Get PDFThe role of MCPIP1 in insulin-secreting cells: comparison with SPL and HC mechanisms of action
Get PDFVenöse Luftembolie im Rahmen der halbsitzenden Position bei neurochirurgischen Operationen: Abhängigkeit der Inzidenz und des Schweregrades von der verwendeten Überwachungsmethode
No full textHuman thioredoxin, a damageassociated molecular pattern and Malassezia-crossreactive autoallergen, modulates immune responses via the C-type lectin receptors Dectin-1 and Dectin-2
No full textHuman thioredoxin (hTrx), which can be secreted from cells upon stress, functions in allergic skin inflammation as a T cell antigen due to homology and cross-reactivity with the fungal allergen Mala s13 of the skin-colonizing yeast Malassezia sympodialis. Recent studies have shown that cell wall polysaccharides of Malassezia are detected by the immune system via the C-type lectin receptors Dectin-1 and Dectin-2, which are expressed on myeloid cells. Therefore, this study aimed to investigate a putative interaction between Dectin-1, Dectin-2 and the allergens Mala s13 and hTrx. Stimulation of human monocyte-derived dendritic cells or macrophages with Mala s13 or hTrx resulted in remarkable secretion of IL-1beta and IL-23. Blocking experiments suggest that hTrx induces IL-23 by Dectin-1 binding and IL-1beta by binding to either Dectin-1 or Dectin-2. Regarding Mala s13, Dectin-1 appears to be involved in IL-1beta signaling. Interference of Syk kinase function was performed to investigate downstream signaling, which led to diminished hTrx responses. In our experiments, we observed rapid internalization of Mala s13 and hTrx upon cell contact and we were able to confirm direct interaction with Dectin-1 as well as Dectin-2 applying a fusion protein screening platform. We hypothesize that this cytokine response may result in a Th2/Th17-polarizing milieu, which may play a key role during the allergic sensitization in the skin, where allergen presentation to T cells is accompanied by microbial colonization and skin inflammatio
Influence of glucose and insulin in human adipogenic differentiation models with adipose-derived stem cells.
No full textAutologous fat grafting represents an attractive source for tissue engineering
applications in the field of reconstructive medicine. However, in adipogenic
differentiation protocols for human adipose-derived stem cells, the concentration
of glucose and insulin varies considerably. With the intent to gain maximum
tissue augmentation, we focused on the late phase of adipogenesis. In this study,
we modified the differentiation protocol for adipose-derived stem cells by
prolongation of the induction period and the application highly concentrated
glucose and insulin. Human adipose-derived stem cells were isolated from
subcutaneous depots and differentiated in a standard induction medium for the
first two weeks, followed by two weeks with varying glucose and insulin
concentrations. Morphological changes assessed using Oil-Red-O staining were
examined for corresponding alterations in the expression of the adipogenic
markers peroxisome proliferator-activated receptor gamma (PPARgamma) and
lipoprotein lipase (LPL). Furthermore, glucose and lactate levels in conditioned
media were monitored over the period of differentiation. We found high-glucose
media increasing the level of lipid accumulation and the size of single droplets
whereas insulin significantly showed a dose-dependent negative effect on fat
storage. However, whereas high glucose stimulated PPARgamma transcription,
expression levels in insulin-treated cells remained constant. Results permit
assumptions that a high-glucose medium intensifies the degree of differentiation
in mature adipocytes providing conditions to promote graft volume while we have
identified highly concentrated insulin treatment as an inhibitor of lipid storage
in the late adipogenic differentiatio
Psychometric Properties of the German Version of the Pulmonary- Specific Quality-of-Life Scale in Lung Transplant Patients
No full textThe Pulmonary-Specific Quality-of-Life Scale (PQLS) is a validated self-report questionnaire assessing health-related quality of life (HRQoL) in patients with end-stage lung disease awaiting lung transplantation. The aim of our study was to evaluate the psychometric properties of the German version of the PQLS. One hundred and forty patients awaiting lung transplantation (55% men) with a median age of 53 years [Interquartile range (IQR) 13] answered the PQLS. A group of the participants (n = 43) was evaluated again 1 year later after transplantation. A confirmatory factor analysis (CFA) of the PQLS was conducted to test the three-factor structure of the PQLS. We examined the internal consistency of the scales using Cronbach's alpha. Convergent validity was explored through correlations with generic measures of HRQoL [Short-Form 8 Health Survey (SF-8), 10-item quality of life (QoL) scale], measures of depression (nine-item Patient Health Questionnaire-Depression Scale), anxiety (Generalized Anxiety Scale), and measures of lung disease severity (supplemental oxygen use, stairway steps). In the group of 43 patients assessed before and after transplantation, sensitivity to change was explored. The CFA confirmed the three-factor model with an acceptable fit. The PQLS total and the three subscale scores "task interference," "psychological," and "physical" showed acceptable internal consistency. The PQLS and its subscales showed a significant negative correlation with the 10-item QoL measure and the physical component score of the SF-8, whereas the mental component score of the SF-8 showed a significant negative correlation only with the PQLS subscale "psychological." Negative correlation was found due to the opposed alignment of the PQLS compared to the 10-item QoL and the SF-8. Symptoms of depression and anxiety were significantly and positively correlated with the subscale "psychological." Measures of lung disease severity also exhibited a significant positive correlation with the subscales "task interference" and "physical" but not "psychological." In patients 1 year after a successful transplantation, the PQLS scores were significantly reduced by 50%. The three-factor structure of the PQLS could be replicated using CFA. The results indicate good reliability, validity, and sensitivity to change of the German version of the PQL
Cytomegalovirus-specific CD8+ T-cells are associated with a reduced incidence of early relapse after allogeneic stem cell transplantation
No full textLeukemia relapse is the main cause for mortality after allogeneic stem cell transplantation (allo-SCT). Donor-derived allo-immune responses eliminate the residual host hematopoiesis and protect against relapse. Cytomegalovirus (CMV) reactivation (CMV-R) after allo-SCT may trigger anti-leukemic effects. The impact of CMV-specific CD8+ T-cells (CMVCTLs) on the outcome after allo-SCT is currently unknown. Here, we studied the relationship between CMV-CTLs, overall T-cell reconstitution and relapse incidence in 103 patients with
acute leukemia (n = 91) or myelodysplastic syndrome (n = 12) following CMV-seropositive recipient/donor (R+/D+) allo-SCT. Patients were subdivided based on the presence or absence of CMV-CTLs at 3 months after allo-SCT. Presence of CMV-CTLs was associated with preceding CMV-R and a fast T-cell reconstitution. Univariate analysis showed a significantly lower 1-, 2- and 5-year cumulative incidence of relapse (CIR) in patients with CMVCTLs compared to those without CMV-CTLs. Multivariable regression analysis of the outcome performed with other relevant parameters chosen from univariate analysis revealed that presence of CMV-CTLs and chronic graft-versus-host disease (cGvHD) were the only independent factors associated with a low CIR. Onset of relapse was significantly later in patients with CMV-CTLs (median 489 days) than in in those without (median 152 days, p =0.041) during a five-year follow-up. Presence of CMV-CTLs was associated with a lower incidence of early relapses (1 and 2-years), while cGvHD lead to a lower incidence of late
relapses (2 to 5-years). In conclusion, our data show that CMV-CTLs indicate a functional immune-reconstitution protective against early relapse
