UAX Archive (Univ. Alfonso X el Sabi)
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Single-nucleus RNA sequencing identifies a novel tenogenic heterologous differentiation in endometrial carcinosarcomas: implications for diagnosis and tumor classification.
Full text linkCarcinosarcomas (CSs) are aggressive biphasic tumors characterized by epithelial and mesenchymal components, whose histogenesis and differentiation dynamics remain poorly understood. We present single-nucleus RNA sequencing (snRNA-seq) analysis of six CSs (five endometrial and one ovarian) and two normal endometrial samples, profiling over 96,298 cells. By integrating transcriptomic data with inferred copy number variations (CNVs), immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and in situ hybridization (ISH) validation, we resolved the complex cellular architecture of these tumors, identified lineage-specific programs, and revealed unexpected differentiation trajectories. snRNA-seq was used to further refine the histopathological classification of three cases by uncovering heterologous differentiation not previously recognized: one rhabdomyogenic, one osteogenic, and, notably, one exhibiting a novel tenogenic program, defined by the expression of SCX, MKX, and TNMD. All CSs displayed a prominent mesenchymal compartment comprising both undifferentiated fibroblast-like cells and distinct lineage committed populations, including rhabdomyoblasts (Rhab), tenoblasts (Teno), osteoblasts (Osteo), and chondroblasts (Chond). In some tumors, multiple mesenchymal identities co-existed, and in others, differentiation gradients (e.g. immature versus mature rhabdomyoblasts) were observed. These patterns underscore the cellular plasticity and multilineage potential of the sarcomatous component. Furthermore, the expression of specialized interface markers (COL22A1, NCAM1, ACAN, CHRNG, MUSK) suggests that some tumors use structured developmental programs reminiscent of the muscle-tendon junction, enthesis, or neuromuscular junction. CNV analysis revealed tumor-specific genomic alterations with clonal and subclonal patterns linked to differentiation state, which were validated by FISH. Altogether, this study demonstrates that CSs are not static biphasic tumors but rather complex ecosystems with extensive developmental plasticity. Our findings redefine their classification and support the use of single-nucleus approaches to uncover hidden differentiation trajectories in highly heterogeneous cancers, including the discovery of a previously unreported tenogenic lineage. Our results challenge the diagnosis of homologous CS when only morphological criteria are applied. © 2026 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.ye
Análisis del Conocimiento Didáctico del Contenido (CDC) en profesores de pedagogía en Educación Física en Chile.
Full text linkIntroducción: el artículo presentó el conocimiento didáctico del contenido como clave para integrar saber disciplinar y saber pedagógico en educación física dentro del sistema escolar chileno. se contextualizó su pertinencia por las demandas curriculares y la necesidad de experiencias motrices significativas.
Objetivo: Este estudio analiza el Conocimiento Didáctico del Contenido del profesorado de Educación Física en Chile y sus implicancias para la enseñanza escolar.
Metodología: Participaron 323 docentes (37.7±7.5 años; 15.7±7.5 años de experiencia) de establecimientos públicos, subvencionados y privados. Se aplicó el Cuestionario de CDC en EF (35 ítems; 7 dimensiones). Los análisis incluyeron estadística descriptiva, correlaciones de Pearson y k-means, además de ANOVA por sexo y tipo de establecimiento.
Resultados: Los puntajes más altos se observaron en Conocimiento sobre currículo, Relación teoría-práctica y Dominio de la clase (~3.96–3.99), mientras que los más bajos fueron Conocimiento pedagógico general (M=3.03), Estrategias de enseñanza (M=2.99) y Evaluación (M=2.98). Las dimensiones se correlacionaron positivamente, destacando Relación teoría-práctica–Dominio de la clase (r=.81) y Currículo–Dominio de la clase (r=.77), evidenciando la integración del CDC. El clustering identificó tres perfiles: uno equilibrado/alto (Cluster 3), uno intermedio con fortaleza práctica (Cluster 1) y uno con déficits en estrategias y evaluación (Cluster 2).
Discusión: los hallazgos coincidieron con la literatura que reportó fortaleza operativa y debilidad pedagógica, subrayando la necesidad de reforzar transposición didáctica y retroalimentación formativa para aprendizajes significativos.
Conclusiones: se recomienda priorizar desarrollo profesional focalizado en diseño didáctico y evaluación formativa para potenciar la enseñanza de educación física en contextos diversos.ye
Combined creatine and β-hydroxy-β-methylbutyrate supplementation with integral conditioning exercise enhances functional performance and metabolic health in physically active older adults: A randomized controlled crossover trial
Full text linkBackground Combined creatine monohydrate (CRE) and β-hydroxy-β-methylbutyrate (HMB) supplementation may counteract age-related declines in functional capacity, yet evidence in physically active older adults is scarce.
Objective To investigate the effects of six weeks of CRE+HMB supplementation integrated with a supervised multicomponent exercise program on functional performance, metabolic efficiency, and physiological health in older adults.
Methods Thirty physically active adults aged≥60 years (20 men, 10 women) completed a randomized, double-blind, placebo-controlled crossover trial involving two 6-week intervention phases (CRE+HMB or placebo), separated by a 3-week
washout. The exercise program (4 sessions/week) combined strength, endurance, and coordination training. Functional tests
(4-m gait speed, 5-repetition sit-to-stand, Timed Up and Go, 400-m walk), metabolic indices, and cardiopulmonary and
inflammatory markers were assessed pre- and post-intervention.
Results CRE+HMB significantly improved gait speed, sit-to-stand, TUG, and 400-m walk (p<0.05), with large effect sizes
(η²p=0.15–0.29). Basal metabolic rate and metabolic rate index increased, while visceral adiposity showed favorable trends.
Women exhibited reductions in diastolic blood pressure and higher expiratory strength; men showed a transient rise in endothelial protein C receptor (EPCR). No period, sequence, or carryover effects were detected.
Conclusions Six weeks of CRE+HMB supplementation integrated with supervised multicomponent training enhanced
mobility, metabolic efficiency, and selected physiological outcomes in physically active older adults. This strategy represents
a safe, feasible, and practical approach to sustain functional independence and metabolic health with agingye
The Effect of an Immersive Virtual Reality Physical Activity Intervention on Anthropometric Variables, Physical Fitness, and Blood Pressure in College Students: A Randomized Controlled Trial.
Full text linkBackground/Objectives: University students exhibit high levels of sedentary behavior and low adherence to physical activity recommendations, and immersive virtual reality (IVR) represents an innovative strategy to increase physical activity participation. The aim of this study was to evaluate the effect of a physical activity intervention using IVR on anthropometric variables, physical fitness, and blood pressure in university students. Methods: A randomized controlled trial was conducted with 60 participants (30 control, 30 intervention) over 12 weeks. The intervention group performed three weekly exercise sessions using IVR, while the control group maintained their usual activity. BMI, waist and hip circumferences, handgrip strength, cardiorespiratory fitness, and blood pressure were assessed. Baseline characteristics between groups were compared using Student's t-test. The effect of the intervention was analyzed using analysis of covariance adjusted for baseline values. Sensitivity analyses were performed to assess between-group changes, and subgroup analyses were conducted to determine the impact of sex. Results: The intervention produced significant improvements in cardiorespiratory fitness (VO2 and the 20 m shuttle run test); no significant changes were observed in anthropometric variables, strength, or blood pressure. Conclusions: A 12-week intervention with immersive virtual reality-based physical training improves cardiorespiratory fitness in university students, representing a promising tool for health promotion in this populationye
Key competencies in sports management: A comparative analysis between public and private entities in Spain
Full text linkCalidad de la información sanitaria sobre productos farmacéuticos en redes sociales audiovisuales: influencia del carácter educativo
No full textBACKGROUND // Audiovisual social media platforms are becoming increasingly prominent as sources of health information. Pharmacists are gaining visibility as content creators in this space; however, the quality of the information they share about pharmaceutical products remains underexplored. The objective of this paper was to assess the quality of information on medications disseminated by pharmacists on audiovisual social media platforms and to analyze the influence of educational framing and declared advertising on that quality. METHODS // A cross-sectional observational study was conducted. A total of 755 videos were collected in March 2024 from the ten most-followed pharmacist accounts (≥1,000 followers) on Instagram, TikTok, and YouTube. Two independent reviewers evaluated the quality using the abbreviated DISCERN scale (1-5). Educational content (yes/no) and declared advertising were coded. Robust linear regression models were applied to estimate the association between predictors and DISCERN scores. RESULTS // Overall quality was moderate to low (mean DISCERN score: 2.43±0.40), with no significant statistical differences across platforms (p=0.327). Educational videos (28.2%) achieved higher scores (2.62±0.38) compared to non-educational ones (2.34±0.39; p<0.001). Declared advertising decreased quality scores by -0.30 points (p<0.001), with a stronger effect observed on TikTok (-0.50). CONCLUSIONS // Educational framing consistently enhances the informational quality of pharmacist-generated content, regardless of the platform, whereas declared advertising undermines it, especially on TikTok. Promoting evidence-based micro-educational content and enforcing transparent sponsorship labeling may improve the reliability of pharmaceutical information on social media
Gender-based differences in telomere attrition and long-term respiratory dysfunction in COVID-19 ICU survivors one year post-infection: implications for aging-associated pulmonary decline
No full textIntroduction: A significant proportion of COVID-19 Intensive Care Unit (ICU) survivors develop long-term respiratory complications, including pulmonary fibrosis. Telomere attrition, a marker of cellular senescence, has emerged as a potential biomarker for post-COVID-19 sequelae. This study investigated the association between peripheral blood relative telomere length (RTL) and long-term pulmonary outcomes in COVID-19 ICU survivors, with a specific focus on gender-specific differences.
Methods: ICU-admitted COVID-19 patients were followed for at least one year post-discharge. RTL was quantified from peripheral blood using monochromatic multiplex quantitative PCR (MMqPCR) at hospital admission and one-year post-discharge. Primary outcomes were respiratory symptoms and diffuse parenchymal lung disease (DPLD), assessed via imaging. Data were analyzed using gender-stratified generalized linear models, adjusted for clinical covariates.
Results: At one year, 43.8% of patients reported respiratory symptoms and 23.9% developed DPLD. A total of 73 ICU survivors were included, with 51 men and 22 women. At one year, 43.8% of patients reported respiratory symptoms and 23.9% developed DPLD. Longitudinal analysis showed significant RTL shortening in both men and women who underwent IMV (p=0.011 and p=0.016, respectively), and in men who needed pronation during their ICU stay (p=0.037). Regarding one-year symptoms, in women, repeated-measures analysis showed an association with persistent respiratory symptoms, particularly in those who needed pronation during their ICU stay [adjusted arithmetic mean ratio (aAMR)=0.73) (95%CI=0.60-0.90); p=0.003]. At follow-up, women who had undergone pronation and had shorter RTL continued to show a higher prevalence of symptoms [aAMR= 0.66 (0.58-0.76); p< 0.001]. In contrast, men with shorter RTL at one-year post-discharge had an association with the presence of DPLD [aAMR = 0.64 (0.50-0.81); p = 0.001]. This association in men was significant particularly among those who required IMV [aAMR= 0.61 (0.49-0.76); p< 0.001] or prone positioning [aAMR= 0.56 (0.44-0.71); p= 0.016].
Discussion: These findings underscore the role of telomere attrition as a sex-specific biomarker of aging-associated pulmonary vulnerability in the aftermath of critical COVID-19 illness. RTL may serve as a prognostic marker for long-term respiratory sequelae, potentially guiding risk stratification and individualized follow-up strategies in post-ICU COVID-19 survivors.yesFront. Immunol., 06 January 2026 Sec. Cytokines and Soluble Mediators in Immunity Volume 16 - 2025 | https://doi.org/10.3389/fimmu.2025.168145
Sedentary behavior and joint mobility in university students a sex comparison.
Full text linkIntroducción: Los estudiantes universitarios presentan altos niveles de conducta sedentaria (CS), lo que podría afectar negativamente la salud musculoesquelética, incluyendo el rango de movimiento articular (ROM). Sin embargo, la evidencia en poblaciones jóvenes y aparentemente sanas es limitada.
Objetivo: Analizar la relación entre la conducta sedentaria y el ROM de la articulación de la cadera en estudiantes universitarios chilenos, considerando las diferencias según sexo. Métodos: Se realizó un estudio transversal con 189 estudiantes (89 hombres, 100 mujeres; edad media: 21.6 ± 2.4 años). La CS se evaluó mediante el cuestionario IPAQ corto, y el ROM de cadera (flexión, extensión, abducción) se midió con un goniómetro digital bajo condiciones estandarizadas.
Resultados: El tiempo promedio de CS fue de 691 ± 42.6 minutos/día, superando los umbrales comúnmente utilizados para definir sedentarismo. No se encontraron correlaciones significativas entre CS y ROM. Las mujeres mostraron mayor ROM en flexión y abducción de cadera que los hombres (p < 0.05), sin diferencias en extensión.
Conclusión: A pesar del alto nivel de CS en esta población universitaria, no se observó asociación con el ROM de cadera. Las diferencias por sexo coinciden con variaciones anatómicas y conductuales. Se requieren estudios longitudinales con medidas objetivas para evaluar el impacto de la CS en la salud articular.ye