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Porous silicon nanotube bundles as nanocarriers for small interfering RNA delivery
No full textWe describe in this work an evaluation of bundles of hollow mesoporous silicon nanotubes to facilitate transfection of HeLa cells using small interfering RNA designed to knock down expression of Enhanced Green Fluorescent Protein (eGFP). These experiments entail direct visualization of the nanotube bundles associated with the cells using both scanning electron microscopy (SEM) and confocal fluorescence imaging. These nanotube bundles are generated by surface modification of nanotube arrays with aminopropyl†triethoxysilane (APTES), followed by their ultrasonication in water, to create the amine†terminated structures capable of electrostatic conjugation of siRNA at an efficiency of 23%–50% (depending on initial siRNA concentration). Delivery and transfection to HeLa cells are verified by quantification of fluorescence imaging; an average percent knockdown of ∼50% eGFP is achieved. As nanoscale drug delivery vehicles are expected to be resorbed in clinical use, we also assess SiNT bundle degradation during the above in vitro timescale using scanning and transmission electron (TEM) microscopies. We conclude with a brief discussion of challenges and opportunities in future experiments involving this platform
Geochemical comparison of cenomanian post-buda rock in south Texas and Big Bend Ranch State Park
No full textThis study was an attempt to utilize a handheld LIBS to study mudrocks. The LIBS was tested on the Ernst Member of the Boquillas in Big Bend Ranch State Park and in the laboratory on the Swenson 1H core from McMullen County. The analyses on 5 Ernst outcrops found that samples need to be pelletized for optimal results with the LIBS, so it is not a useful field tool, but the XRF analyses found that the outcrops were not affected by hydrothermal fluids from the Solitario. Analyses of the core found an initial poor correlation between the handheld XRF and LIBS, which was improved after calibration of the LIBS and pelletizing the samples. These results were used to determine redox conditions and to correlate to nearby cores. The light element detection capability of the LIBS suggests potential for the study of mudrocks, but not as a stand-alone tool
Overlap of spike and ripple propagation onset predicts surgical outcome in epilepsy
No full textObjective: Interictal biomarkers are critical for identifying the epileptogenic focus. However, spikes and ripples lack specificity while fast ripples lack sensitivity. These biomarkers propagate from more epileptogenic onset to areas of spread. The pathophysiological mechanism of these propagations is elusive. Here, we examine zones where spikes and high frequency oscillations co-occur (SHFO), the spatiotemporal propagations of spikes, ripples, and fast ripples, and evaluate the spike�ripple onset overlap (SRO) as an epilepsy biomarker. Methods: We retrospectively analyzed intracranial EEG data from 41 patients with drug-resistant epilepsy. We mapped propagations of spikes, ripples, and fast ripples, and identified their onset and spread zones, as well as SHFO and SRO. We then estimated the SRO prognostic value in predicting surgical outcome and compared it to onset and spread zones of spike, ripple, and fast ripple propagations, and SHFO. Results: We detected spikes and ripples in all patients and fast ripples in 12 patients (29%). We observed spike and ripple propagations in 40 (98%) patients. Spike and ripple onsets overlapped in 35 (85%) patients. In good outcome patients, SRO showed higher specificity and precision (p?<?0.05) in predicting resection compared to onset and zones of spikes, ripples, and SHFO. Only SRO resection predicted outcome (p?=?0.01) with positive and negative predictive values of 82% and 57%, respectively. Interpretation: SRO is a specific and precise biomarker of the epileptogenic zone whose removal predicts outcome. SRO is present in most patients with drug-resistant epilepsy. Such a biomarker may reduce prolonged intracranial monitoring and improve outcome
Using Genetic Data to Determine Origin for Out‐Migrating Smolt and Returning Adult Steelhead Trout (Oncorhynchus mykiss) in a Southeast Alaska Drainage
No full textOncorhynchus mykiss is a partially migratory salmonid species, and many migratory populations (known as steelhead) have declined in recent decades in the western United States and Canada. Closely related resident populations (known as rainbow trout) may be an effective resource in the recovery of these declining migratory populations. However, the extent to which different populations of resident rainbow trout produce migratory individuals and how likely these individuals are to return as adults to spawn remains unknown. One limitation to answering these questions is the identification of loci that accurately segregate between migratory and resident populations. To address this limitation, we used existing genomic data from a well-studied population of O. mykiss from Southeastern Alaska (Sashin Creek) to identify loci that segregate between phenotypes. We then utilized Double Mismatch Allele-Specific qPCR (DMAS-qPCR) to genotype 233 smolts out-migrating from Sashin Creek and 99 returning adult steelhead trout across a five-year period to determine (a) the origin of out-migrating smolts and returning adults and (b) to quantify the extent to which the resident population contributes to the migratory population. Our results show that 37.3% of out-migrating smolts were produced from resident parents, whereas 19.3% of returning adults had resident parents. Ultimately, these results demonstrate that resident populations of rainbow trout produce migrant offspring that successfully complete their migration and return to spawn, increasing population sizes and likely improving genetic diversity. Therefore, conservation efforts should consider landlocked resident populations for producing smolts when developing recovery plans for migratory steelhead populations
Understanding Barriers to Timely Enrollment of Early Intervention Services for Children who are Deaf and Hard of Hearing
No full textAlthough the Joint Committee on Infant Hearing guidelines recommend that children who are deaf and hard of hearing should begin early intervention by six months of age, prior work has revealed a substantial percentage of children who receive a diagnosis of hearing loss by three months of age but do not enroll in early intervention by six months of age (Grey et al., 2022). To further understand barriers to enrollment in early intervention for these families, we completed qualitative semi-structured interviews with 10 caregivers of children who were diagnosed with hearing loss by three months of age but did not begin early intervention by six months. We recruited from participants in Grey et al. (2022). Interviews were coded using the Bioecological Model of Human Development (Bronfenbrenner and Morris, 2007) as a guiding framework. The interviews revealed widespread barriers encountered by families of children who are deaf and hard of hearing across ecological systems, ranging from child characteristics to macro-level issues like insurance coverage. To ensure that all children who are diagnosed with hearing loss have timely access to early intervention, changes to current policy and practice are needed across multiple ecological systems
Biofeedback and Exercise Load Affect Accuracy of Tongue Strength Exercise Performance
No full textRehabilitative exercises require precise movement coordination and target accuracy for optimal effectiveness. This paper explores the impact of tongue strength exercises (TSE) performance accuracy on exercise outcomes, adherence, and participant confidence and motivation. An 8-week randomized clinical trial included 84 typically aging participants divided into four groups defined by access to biofeedback (present/absent) and TSE intensity dosing (maximal/submaximal) during a home exercise program (HEP). Retention, training, and HEP accuracy were tracked at biweekly visits and during HEP for participants with access to a biofeedback device. Associations with tongue strength outcomes, participant factors, biofeedback, and intensity dosing were analyzed. Exercise accuracy measures did not contribute to tongue strength outcomes at the end of 8 weeks. Increased training accuracy (less practice required to achieve competency) was associated with higher participant confidence and better adherence to the HEP. The presence of biofeedback was associated with reduced adherence but better retention accuracy, while maximal intensity was associated with improvements in all accuracy measures compared to submaximal intensity exercise. These findings in typically aging participants suggest the need for tailored approaches in swallowing-related exercise programs, given the effects of biofeedback and exercise intensity on motor learning and exercise retention. Accuracy performance and its effect on clinical outcomes warrants study in clinical populations with dysphagia and with various rehabilitative approaches. Trial Registration Clincialtrials.gov: NCT0480955
Acceptance and self-protection in government, commercial, and interpersonal surveillance contexts: An exploratory study
No full textDigital surveillance is pervasive in cyberspace, with various parties continuously monitoring online activities. The ways in which internet users perceive and respond to such surveillance across overlapping contexts warrants deeper exploration. This study delves into the acceptance of digital surveillance by internet users and their subsequent self-protective actions against it in three distinct contexts: government, commercial, and interpersonal surveillance. Survey responses collected from 356 internet users in the U.S. showed that acceptance levels for surveillance varied between institutional and interpersonal contexts. However, the degree of self-protection remained consistent across all three contexts. Privacy concerns, algorithm awareness, and perceived privacy control played nuanced roles to both surveillance acceptance and self-protection measures in each context. Interestingly, political orientation emerged as a significant moderating factor on surveillance acceptance. Conservative-leaning participants were less accepting of surveillance overall, especially government surveillance. For conservatives, higher privacy concerns meant less acceptance of both government and corporate surveillance. Liberals’ acceptance levels remained fairly consistent and were less affected by privacy concerns. These findings underscore the significance of contextual differences in privacy and surveillance research and provide implications for refining the existing theoretical frameworks
Entre las dos Coreas: la formulación de la identidad (trans)nacional de la comunidad “coreana†de Cuba en Jerónimo (2019)
No full textEste artÃculo explora el proceso de la diáspora coreana en Cuba mediante el análisis textual del largometraje Jerónimo (2019, Joseph Juhn). Aquà se aborda la idea de la diáspora como un fenómeno transnacional, en tanto que se trata de las vivencias de bloques poblacionales que residen en un territorio, mientras continúan manteniendo alguna forma de conexión con su estado de origen. Los inmigrantes coreanos en Cuba presentan particularidades en su formulación identitaria debido a la compleja relación con las dos Coreas
Centering Racial Health Equity in Systematic Reviews-Paper 1: Introduction to the Series.
No full textObjectives Systematic reviews hold immense promise as tools to highlight evidence-based practices that can reduce or aim to eliminate racial health disparities. Currently, consensus on centering racial health equity in systematic reviews and other evidence synthesis products is lacking. Centering racial health equity implies concentrating or focusing attention on health equity in ways that bring attention to the perspectives or needs of groups that are typically marginalized. Study Design and Setting This Cochrane US Network team and colleagues, with the guidance of a steering committee, sought to understand the views of varied interest holders through semistructured interviews and conducted evidence syntheses addressing (1) definitions of racial health equity, (2) logic models and frameworks to centering racial health equity, (3) interventions to reduce racial health inequities, and (4) interest holder engagement in evidence syntheses. Our methods and teams include a primarily American and Canadian lens; however, findings and insights derived from this work are applicable to any region in which racial or ethnic discrimination and disparities in care due to structural causes exist. Results In this series, we explain why centering racial health equity matters and what gaps exist and may need to be prioritized. The interviews and systematic reviews identified numerous gaps to address racial health equity that require changes not merely to evidence synthesis practices but also to the underlying evidence ecosystem. These changes include increasing representation, establishing foundational guidance (on definitions and causal mechanisms and models, building a substantive evidence base on racial health equity, strengthening methods guidance, disseminating and implementing results, and sustaining new practices). Conclusion Centering racial health equity requires consensus on the part of key interest holders. As part of the next steps in building consensus, the manifold gaps identified by this series of papers need to be prioritized. Given the resource constraints, changes in norms around systematic reviews are most likely to occur when evidence-based standards for success are clearly established and the benefits of centering racial health equity are apparent
Effectiveness of Trained Community Lay Workers on Glycemic Control, Knowledge, and Self-Efficacy Among Agricultural Workers with Diabetes in the Texas Panhandle
No full textSystemic health barriers, economic challenges, and lack of follow-up care exacerbate self-management of chronic diseases like diabetes among Hispanic agricultural workers. The primary objective of this pilot project was to determine the benefit of using community coaches to decrease A1C levels and increase diabetes knowledge among agricultural workers with diagnosed diabetes in the Texas Panhandle. A longitudinal study design with two phases was used to create, deliver, and evaluate a diabetes coaching program. Phase 1 was the development of the program and community coaches training (n = 4). In Phase 2, the coaches then delivered the program over 12 weeks to thirteen clients. Phase 1: All coaches were Hispanic females, 28.3 (SD 3.8) years of age, half had at most a high school education level and the other half had a vocational certification (n = 4). Mean DKQ-24 score was 54.2% (SD = 29.7) at baseline and 75.0% (SD = 31.4) after training (t (4) = 4.6, P < 0.05). We observed a very large difference between mean baseline and exit DKQ-24 scores relative to the pooled standard deviation, resulting in an effect size estimate of 0.59 indicative of a medium to large learning effect. Phase 2: Clients were Hispanic Spanish-speaking, predominantly female (55%), 44.4 (SD 6.8) years of age with at most a high school level of education (88.9%) and occupations varied from dairy farm worker (33.3%), meat processing worker (33.3%), and other agriculture or manufacturing position (33.3%). The mean SKILLD score was 40.0% (SD = 28.7) at baseline, increasing to 72.2% (SD = 25.4) at 12 weeks upon completion of the coaching program (t (9) = 2.956, P < 0.05). We observed a very large difference between mean baseline and exit SKILLD scores relative to the pooled standard deviation, resulting in an effect size estimate of 1.13 indicative of a large learning effect. The mean A1C levels at baseline screening was 8.3% (SD = 3.0) and 7.6% (SD = 3.0) at exit screening, representing a 0.7% decrease (p = 0.4730). No statistically significant differences were observed between depression (p = 0.786) or anxiety (p = 1.000) measures at baseline compared to exit. Training and coaching programs for hard-to-reach agricultural and meat processing workers must be culturally, linguistically, and literacy appropriate for both coaches and clients. The program must be feasible and sustainable, focus on empowering community members, capitalize on technological advances and persisting new-normals from the COVID-19 pandemic as well as dismantle common systemic barriers to health and understanding lived-experiences of agricultural working populations in rural regions