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Delineating Variations in TGF-β–Induced miR-145 Expression Across Epithelial Cell Line Models for Cystic Fibrosis
Full text link2025 Summer Expo Poster Presentation Works in Progresshttps://digitalcommons.library.uab.edu/sp-expo/1139/thumbnail.jp
Identification of the Gd-IgA1 Autoantigen by New Monoclonal Finds Differential NF-kB Signaling
Full text link2025 Summer Expo Poster Presentation Biological & Life Scienceshttps://digitalcommons.library.uab.edu/sp-expo/1142/thumbnail.jp
Confections
Full text linkPatent coloring bookhttps://digitalcommons.library.uab.edu/patentcb/1003/thumbnail.jp
Cognitive Diversity, the Keystone to Effective PtD in the Medical Research and Medical Device Industries
Full text link2025 ASEM 620 Capstone Projecthttps://digitalcommons.library.uab.edu/asem/1008/thumbnail.jp
Single Cell RNASeq of motor cortex in a mouse model of alpha-synuclein pathology
No full textThis Zenodo deposit contains a publicly available description of the Dataset:
Title: Single Cell RNASeq of motor cortex in a mouse model of alpha-synuclein pathology .
Description: This dataset was created with the goal of identifying transcriptomic changes associated with alpha-synuclein pathology in the alpha-synuclein fibril seeding mouse model. The dataset includes single cell RNA Sequencing data from motor cortex of mice injected with alpha-synuclein pre-formed fibrils ( PFF group) or alpha-synuclein monomer as controls ( Control group). The dataset includes subject cohorts with different levels of the following additional experimental factors: sex (male or female); brain hemisphere relative to the PFF injection site (ipsilateral or contralateral); time post-injection (1, 3, 6, or 9 months post-injection).
This dataset is made available to researchers via the ASAP CRN Cloud: cloud.parkinsonsroadmap.org. Instructions for how to request access can be found in the User Manual.
This research was funded by the Aligning Science Across Parkinson\u27s Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson\u27s Research (MJFF).
This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Datase
Data related to Tau, amyloid-beta and alpha-synuclein co-pathologies synergistically enhance neuroinflammation and neuropathology
No full textManuscript Abstract
Alzheimer’s and Parkinson disease pathology can co-occur, with amyloid-beta and phosphorylated tau, found in over half of Parkinson disease cases. Alpha-synuclein inclusions, a hallmark of Parkinson disease, are present in over 30% of Alzheimer’s cases and the co-expression of these pathologies is linked to faster cognitive decline and earlier death. Immune activation is a hallmark of both diseases, but current model systems primarily examine each pathology in isolation. As such, how these co-pathologies interact to drive inflammation and neuronal loss remain poorly understood. To address this, we developed a co-pathology mouse model combining tau, amyloid-beta, and alpha-synuclein. Here, we show that co-pathologies synergistically trigger a distinct and amplified neuroimmune response, marked by robust expansion of CD4+ and CD8+ tissue-resident memory T cells and increased CD68+ microglia, a population of activated, phagocytosing microglia, compared to single pathology brains. These changes were abundant in the hippocampus and cortex, regions showing elevated Aβ protein pathology load and enhanced neuronal loss with co-pathology expression. Our findings demonstrate that co-pathologies act synergistically to enhance immune activation prior to neurodegeneration. This model provides a platform for assessing mixed-pathology mechanisms and identifies key immune cell populations that may drive disease acceleration across Alzheimer’s, Parkinson disease and their related dementias
Prosthetics in the Time of Proust
Full text linkPatent coloring bookhttps://digitalcommons.library.uab.edu/patentcb/1015/thumbnail.jp
Seruat v5 R object for Tuscher et al 2025 bioRxiv preprint snRNA-seq dataset
No full textThis dataset contains single-nucleus RNA-seq results from rat ventral tegmental area brain tissue in a chronic pain model with morphine administration. This experiment contains 28 biological samples, which represent four experimental groups of rats. To assess neuropathic pain response, Complete Freund’s Adjuvant (CFA) or saline vehicle (100 µl) was injected into the plantar surface of one hind paw of male and female rats (n = 3-4/sex/treatment group). Mechanical sensitivity was then evaluated 48 hours and 7 days after the injection using the Von Frey assay. 7 days after CFA injection, rats received either morphine (10mg/kg) or saline injections I.P., generating a 2x2 experimental design (Veh-Sal, Veh-Mor, CFA-Sal, CFA-Mor). One hour after injections, animals were euthanized prior to rapid decapitation. Brains were blocked in 1mm sections, and VTA tissue was obtained and flash frozen, and stored at -80°C prior to nuclei isolation. Following FACS-assisted nuclei isolation and quantification, a total of 8,000 nuclei pooled from two rats (4,000 male nuclei/4,000 female nuclei from the same treatment group) and were loaded into individual wells of the Chromium NextGem Single Cell Chip (10x Genomics, catalog no. 10000121) and processed on the 10X Genomics Chromium Controller. Libraries were constructed according to manufacturer’s instructions for Chromium Next GEM single cell 3’ library and gel bead kit (10x Genomics, v3.1 single index, catalog no. 10000121, version 3 chemistry). Libraries were sequenced on an Illumina NovaSeq6000 (S2 flow cell) to an average read depth of ~71,000 read pairs per nucleus (range 48,000 - 123,000