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The extent and characteristics of DNA transfer between plasmids and chromosomes
No full textPlasmids are extrachromosomal genetic elements that reside in prokaryotes. The acquisition of plasmids encoding beneficial traits can facilitate short-term survival in harsh environmental conditions or long-term adaptation of new ecological niches. Due to their ability to transfer between cells, plasmids are considered agents of gene transfer. Nonetheless, the frequency of DNA transfer between plasmids and chromosomes remains understudied. Using a novel approach for detection of homologous loci between genome-pairs we uncover gene sharing with the chromosome in 1,974 (66%) plasmids residing in 1,016 (78%) taxonomically diverse isolates. The majority of homologous loci correspond to mobile elements, which may be duplicated in the host chromosomes in tens of copies. Neighboring shared genes often encode similar functional categories, indicating the transfer multigene functional units. Rare transfer events of antibiotics resistance genes are observed mainly with mobile elements. The frequent erosion of sequence similarity in homologous regions indicates that the transferred DNA is often devoid of function. DNA transfer between plasmids and chromosomes thus generates genetic variation that is akin to workings of endosymbiotic gene transfer in eukaryote evolution. Our findings imply that plasmid contribution to gene transfer most often corresponds to transfer of the plasmid entity rather than transfer of protein-coding genes between plasmids and chromosomes
DNA-sequence data for the publication: A user-friendly CRISPR/Cas9 system to create loss-of-function mutants in Neurospora crassa
No full textHere you'll find the raw DNA-sequencing data that were used for the analysis of an CRISPR/Cas9 event in the publication "A user-friendly CRISPR/Cas9 system to create loss-of-function mutants in Neurospora crassa". The data are assigned to the different experimental conditions (singel gRNA used, combination of gRNA used) and refer to the corresponding gRNA (gRNA-c1, -c2, -n1 or -n2) used in the experiment
Der methodisch-konstruktive Begriff der Erfahrung in den empirischen Sozialwissenschaften, Untersuchungstermin 20.11. 2023, Audiodatei
No full textDer methodisch-konstruktive Begriff der Erfahrung in den empirischen Sozialwissenschaften, Untersuchungstermin 24.7.2023, Audiodatei
No full textSAXS and DSC data and scripts for publication: Photoinduced bidirectional mesophase transition in vesicles containing azobenzene amphiphiles
No full textHere the small angle X-ray scattering (SAXS) and differential scanning calorimetry (DSC) data, analysis results and analysis scripts are accesssible for the publication "Photoinduced bidirectional mesophase transition in vesicles containing azobenzene amphiphiles". This data is made accessible under DAPHNE4NFDI
Die Deponierungen der Trichterbecherkultur (Appendices 3–4 und Datenbank)
No full textHier sind die zur Publikation "Die Deponierungen der Trichterbecherkultur" (FMSD 21) zugehörigen Appendices 3 (Katalog) und 4 (Listen) sowie die Datenbank abgelegt. Die Datenbank besteht aus den beiden Tabellen "Allgemeine Informationen" (FMSD_21_Mueller_Allgemeine_Informationen.csv) und "Individuelle Informationen" (FMSD_21_Mueller_Indidviduelle_Informationen.csv). Primärschlüsselfeld der Tabelle „Allgemeine Informationen“ ist das Feld „Kat-Nr“, das in der Tabelle „Individuelle Informationen“ als Fremdschlüsselfeld auftaucht
Supplemental material to 'Analysing dust particle size and size distribution on extracted particles by SEM and comparing with light scattering techniques'
No full textThe presented data is supplemental material to the publication 'Analysing dust particle size and size distribution on extracted particles by SEM and comparing with light scattering techniques' (DOI: 10.1002/ppap.202400032) in Plasma Processes and Polymers published by Wiley‐VCH GmbH. The article describes six nearly identical cycles (numbered Set_1-6). In each amorphous hydrogenated carbon nanoparticles formed in a low temperature, low pressure discharge by plasma polymerization. The growth of the particles was monitored and eight consecutive samples per cycle were taken (numbered Extraction_1-8). For each sample scanning electron microscopy images of parts (Part_1-x) of the sample wafers were taken at a fixed magnification of 3000, which equals 12.4 nm per pixel. These images make up the first part . In a next step the particles were identified with the Hough transform-based routine , which is part of . The resulting data is stored in the second part . Lastly, from this analysis the particle size distribution (PSD) is modeled as a normal distribution. While in the publication a subset is presented for discussion, here the full histogram set can be accessed. It is found i
Houses sizes of the LBK settlement Vráble-Veľké Lehemby, Slovakia
No full textThe research project "The Dynamics of Settlement Concentration Processes and Land-Use in Early Farming Communities of the Northwestern Carpathian Basin" investigates settlement patterns and land-use dynamics in the Žitava valley during the Neolithic. Discovered in 2010, the agglomerated Early Neolithic settlement of Vráble proved to be an outstanding site dating between 5250 and 4950 calBCE. The settlement is divided into three distinct groups of houses, which we interpret as neighbourhoods inhabited by different socio-political groups. The settlement history is characterised by a process of low-intensity agglomeration, during which the three neighbourhoods were continuously inhabited and used at the same time. The last phase of the settlement history is characterised by the construction of a ditch system around the south-western neighbourhood, with all entrances to the enclosure facing away from the other parts of the settlement. The social importance, but also the potentially conflictual end of the settlement, is reflected in a series of regular and irregular burials, as well as specific depositional practices within the ditch itself. The life within this agglomerated settlement ended in 4950 calBCE and led to a complete restructuring of the settlement structures in the following Lengyel phases of the Neolithic in the Žitava valley. The German-Slovak research team documented a total of 313 houses with the help of geomagnetic prospection. This plan, together with an extensive coring programme, allowed the spatio-temporal development of the settlement to be reconstructed. It also allowed the reconstruction of the house sizes of 304 houses according to their association with one of the three neighbourhoods of Vráble
Studieren im Wandel der Zeit - ein Podcast
No full textDer Podcast Studieren im Wandel der Zeit bringt drei Generationen von Kieler Geschichtsstudierenden zusammen und lässt diese über ihre Studienzeit reflektieren. Das studentische Projekt zeigt auf, wie moderne Medien in die universitäre Geschichtsschreibung integriert werden können