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La Vie en Roses: Together and Apart, an (Un)braided Model Approach
No full textThis article was originally published in International Textile and Apparel Association Annual Conference Proceedings. The version of record is available at: https://doi.org/10.31274/itaa.18919.
© 2024 The author(s). Published under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.La Vie en roses represents Phase 2 of a multi-phase research project to expand the braided design model. The team of designers created a digitally printed garment with additional fiber art variations of the print to form a three-dimensional component. The final pattern, inspired by a Madame Grès one-piece jacket, allows continuous flow of the engineered print. The team expanded the design with a striped textile print for the lining using “color hearing” through psychomimicry. As much of the print involved roses in shades of pink and peach, the song La Vie en Rose was selected. The striped print is also incorporated as the main jacket background, with additional circular motifs to balance the large floral images. Three-dimensional floral elements, created through dyeless shaped resist, with raw edges and beading, expand the design spatially and texturally. A third silk organza layer can be worn either up or down as a peplum
Towards multi-scale inter-frame attention to improve deep learning tasks
No full textKambhamettu, ChandraAccess to specialized medical screening remains a challenge for individuals with sickle cell disease (SCD), particularly those in low-income and rural communities, where advanced diagnostic tools and expert evaluations are limited. In ophthalmology, Sickle Cell Retinopathy (SCR) diagnosis relies on ophthalmologic evaluation, including Optical Coherence Tomography (OCT) scans, but the manual interpretation is prone to subjectivity, fatigue-induced errors, and inconsistencies across clinicians. Similarly, video-based event analysis—such as reconstructing crime scenes from fragmented surveillance footage—is a time-intensive process that requires manual ordering and interpretation of unordered clips. These challenges highlight the need for automated solutions that enhance medical diagnostics and video-based decision-making. ☐ To address these issues, we propose Multi-scale Inter-frame Attention (MIA), a novel framework that enhances deep learning models for processing volumetric and video datasets. Our approach leverages spatial and spatio-temporal attention mechanisms to improve feature extraction and representation learning. We integrate MIA into two specialized models: the Cross-Scan Attention Transformer (CSAT) for SCR detection and the Sequential Ordering of Frames in Time (SOFT) for video-based action recognition. Experimental results demonstrate that CSAT+MIA outperforms conventional object detection models in diagnosing SCR, while SOFT+MIA enhances action recognition, particularly in temporally shuffled scenarios. ☐ Beyond domain-specific improvements, our research aims to establish a unified deep-learning method capable of capturing both inter-frame and intra-frame relationships for broader applications in medical imaging, surveillance, and video understanding. By integrating multi-scale inter-frame attention, we advance the field of automated diagnosis and event reconstruction, paving the way for more efficient, reliable, and intelligent decision-making systems.University of Delaware, Department of Computer and Information SciencesPh.D
MemorySeq identifies heritable epigenetic phenotypes that initiate cell culture stress tolerance in CHO cells
No full textThis article was originally published in iScience. The version of record is available at:https://doi.org/10.1016/j.isci.2025.113478
Under a Creative Commons license https://creativecommons.org/licenses/by-nc-nd/4.0/During manufacturing batches, Chinese hamster ovary (CHO) cells encounter critical levels of environmental stressors which can significantly reduce cell health and productivity. Therefore, stress tolerance must be considered during selection of a suitable host. In this study, we employ a population-based transcriptomic method, referred to as MemorySeq, and differential gene expression analysis on stress-shocked CHO cells to identify stress responsive biomarkers. These biomarkers exhibit transient and intermediate heritable memory states characteristic of epigenetic switches and transcriptional bursting. Using this workflow, 199 genes were found to exhibit transcriptional variability characteristic of two-state systems with switching that forms four network communities of co-fluctuating genes. These communities were enriched in genes related to the regulation of apoptotic processes, gene expression, and metabolic pathways. Seven genes were identified as promising biomarkers of stress-resistance. Genetic engineering methods may be employed in the future to bias clonal populations toward higher stress tolerance to manufacturing stress.We thank the Expression Systems and Novel Biopharm Materials Team at Millipore Sigma for access to the CHOZN GS / Clone 23 cell line; Dyllan Rives for aiding in cell culture; Molly Wintenberg for providing RNA-seq analysis pipeline for CHO, Sylvain Le Marchand with the Delaware Biotechnology Institute’s Bio-Imaging Center for instruction and experimental planning for smRNA-FISH; Erin Bernberg and Mark Shaw with the Delaware Biotechnology Institute’s Sequencing and Genotyping for aid in RNA extractions and quality verification; Ross Klauer, Emily Doleh, Christopher Pirner for reviewing and editing the paper. This work was supported by funding from the UD College of Engineering. A.S. acknowledges the support of NIH-NIGMS via grant R35GM148351
Contribution of medial septal modulation to spatial working memory through optogenetic manipulations
No full textGriffin, Amy L.Working memory (WM) is a critical function needed to interact with our environment and those around us. WM refers to the ability to hold information “online” and actively manipulate that information for successful use in task-relevant behavior. One way to further examine WM, is by modeling WM in rodents by assessing aspects of spatial working memory (SWM). SWM in rodents refers to the ability to maintain goal-relevant information over a temporal delay and successfully recall that information when it is needed. The underlying neural mechanisms involved in SWM are believed to be orchestrated by the septo-hippocampal theta oscillations. Septo-hippocampal theta oscillations are known to synchronize the hippocampal network through a vast expansion of GABAergic inhibitory influence. The intricate local connections and physiology of the medial septum (MSA) and reciprocal inhibitory influences returning from the hippocampus, pace theta oscillations in the hippocampal formation and temporally organize hippocampal and prefrontal cell assemblies. Septo-hippocampal theta oscillations are reported to offer a method of communication between the prefrontal cortex and hippocampus, of which both are critical for SWM. My hypothesis is that the MSA provides a critical modulatory influence on hippocampal-prefrontal theta communication, which is necessary for optimal SWM performance. To investigate the role of MSA during SWM, I first demonstrate that MSA is crucial for SWM maintenance (Aim 1). Next, I paced hippocampal theta oscillations via MSA optogenetic theta stimulation during short and extended delays to determine whether MSA theta stimulation is beneficial to task performance (Aim 2). Lastly, I examined the impact of MSA theta stimulation on hippocampal-prefrontal communication by assessing theta coherence between the two regions (Aim 3). My results demonstrate that MSA activity is crucial for SWM maintenance during a hippocampal-dependent conditional discrimination working memory task. In addition, I found that MSA theta stimulation during SWM maintenance enhances choice accuracy, specifically on trials where the working memory demand was prolonged. This finding may be explained by observations in Aim 3, of MSA theta stimulation altering dorsal hippocampal (dHPC) and medial prefrontal cortex (mPFC) theta power dynamics and enhancing dHPC-mPFC theta synchrony over the extended delay trials. These results highlight a vital role for septo-hippocampal theta in SWM. Moreover, they extend the implications of theta stimulation for therapeutic interventions by providing foundational evidence for its effects in non-pathological models.University of Delaware, Department of Psychological and Brain SciencesPh.D
TARGETING MESOTHELIN IN PEDIATRIC ACUTE MYELOID LEUKEMIA WITH DUAL ANTIBODY-COATED POLY(LACTIC-CO-GLYCOLIC ACID) NANOPARTICLES
No full textenterAcute myeloid leukemia (AML) is a rare but aggressive cancer, accounting for
approximately seven new cases per 1 million children annually. It arises from the
malignant transformation of hematopoietic stem and progenitor cells, leading to an
accumulation of immature, nonfunctional myeloblasts in the bone marrow. Standard
treatments, such as chemotherapy and stem cell transplantation, have high relapse rates
and significant toxicity, emphasizing the need for more effective therapies.
Immunotherapy has emerged as a promising treatment strategy, however, specific
targeting of AML cells and maintaining extended circulation times of administered
agents remain major challenges. Mesothelin (MSLN) has been identified as an attractive
immunotherapeutic target that is overexpressed in one-third of young adult and pediatric
AML patients with limited expression in normal bone marrow.
Here, we report the design, characterization, and validation of dual antibody coated poly(lactic-co-glycolic acid) (PLGA) nanoparticles (duNPs) that recruit T
lymphocytes to AML to induce specific cytotoxicity of AML cells. PLGA NPs were
coated with anti-MSLN (Amatuximab) and anti-CD3 antibodies (OKT3) and
characterized for size, charge, and concentration. Microscopy analysis revealed duNPs
pull T lymphocytes and MSLN-expressing NOMO-1 cells into clusters to induce
cytotoxicity. Additionally, the duNPs facilitated significantly higher T cell activation
compared to bare NPs, as confirmed by elevated CD69 levels, suggesting the duNPs
successfully engage T cells. To evaluate the efficacy of duNPs in promoting T cell
induced killing of AML cells, the T cells were incubated with AML cells and treated
with increasing amounts of bare NPs or duNPs for 16 hours before assessing cell
viability. duNP treatment selectively killed AML cells in a dose-dependent manner
compared to bare NPs. Collectively, our data suggest that dual antibody-coated NPs are
effective agents to recruit T lymphocytes to MSLN-expressing AML cells, increase T
cell activation, and induce AML cell death.ente
Grant Assistance Program Case Study: Milton Art Crosswalks
No full textThe Town of Milton won three consecutive AARP Community Challenge Grants to install a total of 14 art crosswalks and two benches from 2022 to 2024. Spearheaded by Councilwoman Randi Meredith, these projects involved careful coordination between Town of Milton officials and staff, local artists, residents, volunteers, and the Delaware Department of Transportation (DelDOT). The projects and grant applications resulted from over 5 years of planning and coordination work within the Town of Milton. This case study describes the project design and implementation
Clinical Provider Perspectives on Remote Spirometry and mHealth for COPD
No full textThis article was originally published in Nursing Reports. The version of record is available at: https://doi.org/10.3390/nursrep15110402
Copyright: © 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/).Background/Objectives: COPD is a leading cause of death in the US, with higher morbidity and mortality in rural areas that lack specialized pulmonary care. Mobile health (mHealth) tools, including remote spirometry, may fill this gap, yet healthcare provider (HCP) perspectives on utility and implementation of remote spirometry and mHealth for COPD management in these settings remain unexplored. This study aimed to examine HCPs’ perspectives of mHealth with remote spirometry to inform future implementation in
rural and low medical access settings. Methods: Five HCPs working in rural or medically limited settings in South Carolina participated in a deliberative discussion focus group. A semi-structured interview guide was used to explore insights into feasibility, clinical utility, and implementation needs. Transcripts were analyzed using thematic analysis to identify facilitators, barriers, and implementation considerations. Results: Participants reported that remote spirometry and mHealth had potential to support COPD treatment, increase healthcare access, and support self-management. Key facilitators identified were access to smartphones, potential for individualized COPD care, and visual tools for patient engagement. Barriers included risk of time and workload burden, data information overload, and technological limitations. Participants emphasized the need for team training, thoughtful integration into existing workflows, customizable data displays, and robust support for patient onboarding. Conclusions: Providers viewed mHealth applications with remote spirometry as a valuable tool with potential to improve COPD care but identified critical implementation needs. Participants emphasized that successful integration would require
structured support, user-centered design, and attention to reimbursement and workflow alignment to enhance sustainability and patient/provider engagement.Primary support for this work was funded by the South Carolina Center for Rural and Primary Healthcare as part of the state’s rural health initiative. REDCap at SCTR is supported by the National Center for Advancing Translational Sciences of the National Institutes of Health under Grant Number UL1TR001450
An examination of the therapeutic alliance, treatment engagement, and processing during symptom increases in cognitive processing therapy for posttraumatic stress disorder
No full textHayes, Adele M.Cognitive Processing Therapy (CPT) is an effective, gold-standard treatment for adult posttraumatic stress disorder (PTSD), although some clinicians are reluctant to use the treatment, and the rates of client dropout are high. These challenges often center around periods of increased distress during treatment, as clients engage with and process traumatic experiences. Research has shown the importance of constructive processing and treatment engagement in CPT; however, it is not clear how to facilitate these processes during particularly challenging sessions. This study coded the content of CPT sessions that occurred at maximum PTSD symptom increases for each client. Two observational coding measures (CHANGE and the Working Alliance Inventory-Observer Version-Short Form) were used to better understand what may be happening during these opportunities for change. The sample included 57 adults with PTSD, who received CPT as part of a non-inferiority trial that compared CPT to written exposure therapy (WET), a brief, narrative only condition. This study included the therapeutic alliance, two variables related to processing (cognitive emotional processing, capitalization), and two variables that can interfere with treatment engagement (avoidance, overgeneralization). Bivariate correlations showed that higher alliance scores for the sessions with the maximum PTSD symptom increases were associated with more client cognitive emotional processing, more capitalization, and less overgeneralization. Hierarchical multiple regression analyses showed that more cognitive emotional processing during these sessions predicted a greater number of sessions completed. Finally, latent growth curve modeling showed that higher alliance predicted a greater decrease in the slope of PTSD symptoms from baseline to 24 weeks. These findings may help to identify facilitators of change in CPT, provide practical suggestions to reduce dropout, and highlight the importance of the alliance during treatment. ☐ Keywords: PTSD, Cognitive processing therapy, therapeutic alliance, processing, treatment engagementUniversity of Delaware, Department of Psychological and Brain SciencesPh.D
"Whatever it is, serve it in glass!": color, gender, and class in pressed-glass tableware, 1925-1945
No full textKillian, JackieThis thesis explores colorful pressed glass tableware produced in the United States from 1925 to 1945, objects that illuminate an intersection between industrial innovation, consumer culture, and working-class domestic life. This period covers the pressed-glass tableware industry’s response to the 1925 Exposition Internationale des Arts Décoratifs et Industriels Modernes in Paris with the introduction of colorful, pressed glass tableware set. The project follows an arc through the peak of pressed glass’ popularity in the 1930s through the collapse of demand for colorful pressed tableware in the United States by the end of World War II. By studying these mass-produced objects, this paper examines how working- and middle-class women deployed material and color to craft domestic identities, embody personality and emotion in the kitchen, and mediate their relationship with household labor. ☐ This project begins by outlining technological advancements in glass production in the nineteenth century, particularly the development of mechanically aided pressing which transformed the industry and democratized access to glass. The resulting “glassification” of lower-income consumers implicated pressed glass directly in the inculcation of aspirational consumer groups into larger American value systems emphasizing tradition and history. While pressed glass mimicked expensive cut crystal at the turn of the twentieth century, this thesis argues that during the peak of its popularity in the 1920s and 1930s, companies in the upper Ohio River Valley produced innovative and distinctly modern glass tableware. Parallel to this changing industrial output, lower-income women developed a unique aesthetic language and use for these objects that challenged conceptions of good design and taste originating from wealthy consumers, deploying color and glass to alleviate drudgery and express satisfaction, comfort, and joy in their kitchens and dining rooms. ☐ Drawing on consumer advertising, shelter magazines, and an abundance of material culture evidence, the research demonstrates how glass manufacturers responded to diverse market demands among non-elite consumers. Companies like Fostoria and Heisey positioned their products at the intersection of modernity and tradition, creating forms that spoke to changing American dining practices and domestic spaces. This paper argues that their colorful glass enabled women to imbue gendered household tasks and spaces, especially the kitchen and dining room, with explicitly positive associations. Rather than viewing these mass-produced objects as cheap imitations of elite goods, this work reframes them as emblems of a “joyous materialism” through which women exercised agency, creativity, and care. ☐ The study concludes by examining how external forces created gendered narratives for glass tableware and its socially appropriate uses, arguing that post-war male consumers rejected color and mechanical production due to their explicit associations with female consumers. This analysis illuminates broader themes about gender, class, and consumer culture in early twentieth-century America, while offering new perspectives on how mass-produced objects served as tools for individual expression and identity formation.M.A.University of Delaware, Winterthur Program in American Material Cultur
Normative Data for Single- and Dual-Task Tandem Gait Performance in Collegiate Athletes
No full textThis article was originally published in Sports Medicine. The version of record is available at: https://doi.org/10.1007/s40279-025-02306-2
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.Background
Normative dual-task (concurrent cognitive and motor task) tandem gait has not been developed. Currently, only individual baseline data are used for tandem gait assessment post concussion.
Objective
The object was to (1) determine factors associated with single-task and dual-task tandem gait time among collegiate athletes across multiple institutions, and (2) provide robust normative data for single-task and dual-task tandem gait time based on clinically relevant factors.
Methods
Data were analyzed from 2,137 unique collegiate athletes (19.0 ± 1.1 years, 48.9% female, 23.7% with concussion history) from 2015 to 2022 during pre-injury baseline concussion testing from three universities. Tandem gait was performed under single- and dual-task conditions (serial subtraction by sixes/sevens, spelling five-letter words backward, reciting the months backward). The criteria for being a clinically relevant independent variable was (a) p value < 0.05, and (b) effect estimate of ≥ 1 s. Normative data based on established percentile thresholds were derived and stratified by clinically relevant factors.
Results
None of the single-task tandem gait times were clinically relevant, while sex and contact level were for dual task. Mean (95% confidence interval) for overall single- and dual-task tandem gait times were 12.07 s (11.95, 12.19) and 16.51 s (16.29, 16.73), respectively.
Conclusion
Our results provide robust normative data for single- and dual-task tandem gait stratified by relevant patient factors that can be immediately used by clinicians and future researchers. Future research should compare the use of individual baseline versus normative data for acute concussion tracking.The research reported in this work was supported by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health Award No. R01NS100952 (Dr. Howell). Data collected from the University of Delaware were collected under Award No. 1R21NS122033 and R03NS104377 (Dr. Buckley). The funding bodies were not included or consulted in the interpretation and reporting of the results