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Flow cytometry based directed evolution of cell surface displayed laccase
No full textDirected evolution is a technique applied in protein engineering, which consists of randomly mutating a gene, allowing the improvement of different enzyme properties, which are desired for specific industrial processes. Some of the properties include affinity, solubility, organic solvent resistance, enantioselectivity and thermostability. The probability of succeeding in directed evolution experiment depends on the size of the library generated, because the more variants are present, the higher the chances of detecting an enzyme with improved properties. The throughput represents the main bottleneck in enzyme screening, as most of the methods allow the analysis of 104 variants per round, which is not enough for a library size of 109 variants.
Ultra high-throughput screening (uHTS) enables the analysis of 107 events per hour. It can reduce the time, costs and consumables needed for an applied round of directed evolution. One of its limitations is the use of a flow cytometer, which is expensive and not affordable for everyone. Also uHTS is restricted to single catalytic reactions and their coupling with synthetic fluorophores.
The objective of this project is to develop an uHTS method based on fluorescence activated cell sorting (FACS). The principle of this method is to sort cells expressing the active enzyme variant, which have a fluorescent hydrogel formed on the cell membrane. Successful completion of this protocol will allow the screening of different enzyme classes, for example, laccases, alcohol dehydrogenases, organophosphorus hydrolases. In order to prove that the concept is working, laccase will be used for generating the library, which will then be displayed on the surface of E. coli. The variants which are enzymatically active, will produce the radicals that initiate the polymerization reaction of fluorescent hydrogel, allowing the flow cytometer to sort cells based on the fluorescence emitted. One round of sorting should achieve at least a 3-fold enrichment of the active enzyme population
4/2021 Erste Änderungssatzung zur Prüfungsordnung für den Bachelorstudiengang International Relations der Fakultät Gesellschaft und Ökonomie an der Hochschule Rhein-Waal
No full textOASIS Sprenger
No full textOur goal is to create a water-efficient sprinkler for residential gardens that is easy to use and responsive to weather conditions. The package comes with two wind sensors - i.e. wind vane and anemometer, which are mounted on a pole. The pole can be placed anywhere in the garden as long as the portable sprinkler stays within a range of 100m. When switched on, the controller on the sprinkler gets continuously updated with the current wind speed and direction using wireless transceivers. The sprinkler can also be controlled through an app on a smartphone. Simple commands such as ON/OFF switch, as well as timers for autonomous watering are possible to set up. For example, setting up the system to water the garden at sunrise, which has been suggested by experts as the most effective time to irrigate plants. Morning irrigation, on the one hand, supplies the grass and plants with enough water to sustain high temperature during the daytime and on the other hand, prevents fungal disease growth and different insects from harming the plants during the night by reducing moisture. Therefore, our sustainable irrigation system not only takes into consideration water conservation, but also provides best methods for having healthy plants in a garden. Our competitive advantage is that our sprinkler is made of durable materials and will save household costs as a long-term investment with efficient water usage methods. There are no other sprinklers in Germany that match our design and usability, which makes our product unique in the market. We will start production and sales in Germany with plans to later expand into the European, North American, and Asia-Pacific region
Zugang zum Recht im Zeitalter der Digitalisierung
No full textDer Zugang zum Recht im Zeitalter der Digitalisierung. Was versteht man überhaupt unter dem Zeitalter der Digitalisierung? Der Zugang zum Recht, wird in diesem Zusammenhang, anhand verschiedener Ansätze des Legal Techs dargestellt. Welche Chancen bietet uns Legal Tech und welche Risiken bringen neue Technologien, wie Machine Learning und der Einsatz von künstlicher Intelligenz mit sich? Abschließend wird ein kurzer Ausblick auf die Auswirkungen der Covid-19 Pandemie auf den digitalen Zugang zum Recht beschrieben
15/2021 Fünfte Änderungssatzung zur Satzung der Studierendenschaft der Hochschule Rhein-Waal
No full text22/2021 Prüfungsordnung für den Masterstudiengang Information Engineering and Computer Science an der Hochschule Rhein-Waal
No full text23/2021 Zugangsordnung für den Masterstudiengang Information Engineering and Computer Science der Fakultät Kommunikation und Umwelt an der Hochschule Rhein-Waal
No full textDays of Antidiscrimination - 09.-12. Nov 2020
No full textThis brochure shows the results and impressions of the four-day digital event “Virtual Days of Antidiscrimination” at Rhine-Waal University of Applied Sciences, which took place from November 9 to 12, 2020. The Vice President for Internationalisation and Diversity and the AStA jointly organized this exchange of all university members and, with this report, make visible the voices of the participants, the expertise of the academics and the creative contributions of the students. In addition to the general topic of anti-discrimination, the report also deals specifically with gender and sexual identity, social capital, mental health, and racism and xenophobia
