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Novel insights into human CRISP2: localization in reproductive tissues and sperm, and molecular characterization.
No full textpeer reviewedCRISP2 is enriched in the male reproductive system of mammals and plays roles in spermatogenesis, sperm motility, and fertilization. Although extensively investigated in rodents and boars, human CRISP2 (hCRISP2) remains poorly studied, particularly concerning its localization in testicular and epididymal tissues and its molecular features. In this study, we used immunofluorescence to determine the localization of hCRISP2 in testis, epididymis, and ejaculated sperm. While no expression was observed in the epididymal epithelium, hCRISP2 was detected at different stages during spermatogenesis. Specifically, hCRISP2 was found in the nucleus of primary spermatocytes and of both round and early elongated spermatids. In elongated spermatids, it was additionally observed in the cytoplasm, the flagellum, and the equatorial segment of the acrosome (EqS). The presence of aggregated material with hCRISP2 immunoreactivity in the apical pole of Sertoli cells suggests that most of the hCRISP2 involved in spermatogenesis is phagocytized by these cells during spermiation. In ejaculated sperm, hCRISP2 was found in the cytoplasmic droplet, flagellum, and EqS, consistent with its described roles in sperm motility and gamete fusion. Native and SDS-PAGE combined with western blot analyses depicted the ability of hCRISP2 to form stable high molecular weight complexes and mass spectrometry revealed that these complexes likely consist exclusively of hCRISP2. Furthermore, we showed that hCRISP2 undergoes only limited post-translational modifications. These findings shed light into the dynamic localization of hCRISP2 throughout spermatogenesis and in ejaculated sperm, as well as its molecular features, enhancing our understanding of its pivotal functional roles and relevance for male fertility
Développement d’un outil d’orientation formative et non contraignante – rapport technique
No full textDans le cadre d’une convention avec la Fédération Wallonie-Bruxelles, le présent rapport technique expose la conception et la mise en œuvre d’un dispositif de formation destiné aux professionnels de l’éducation, en lien avec la plateforme numérique Ada (Accompagnement au Développement de ton Avenir). Cette initiative s’inscrit dans une perspective d’orientation scolaire formative, inclusive et non prescriptive, visant à soutenir les élèves du troisième degré de l’enseignement secondaire dans leur transition vers l’enseignement supérieur ou d’autres formes d’insertion socioprofessionnelle.
Le rapport précise les objectifs de la formation conçue par le groupe de travail WP5 : former les enseignants, éducateurs et agents PMS à l’accompagnement des jeunes à l’aide de la plateforme Ada. Celle-ci repose sur trois modules complémentaires — intérêts, compétences et motivation — structurés selon des principes d’exploration personnelle et de dialogue réflexif. L’accent est mis sur l’importance d’une posture d’écoute active et de co-construction avec l’élève, en opposition aux approches prescriptives traditionnelles.
La formation s’organise en deux demi-journées et articule des séquences pédagogiques fondées sur des activités en présentiel et en distanciel. Elle est soutenue par un guide pédagogique exhaustif, conçu comme un outil de référence permettant aux formateurs de s’approprier le dispositif dans ses dimensions conceptuelles, méthodologiques et pratiques. Ce guide intègre des représentations visuelles, des ressources, ainsi que sept annexes thématiques favorisant l’analyse de cas, la formulation de feedbacks éducatifs et la réflexion éthique sur l’accompagnement à l’orientation.
L’ensemble de la formation repose sur une approche intégrative qui valorise la diversité des représentations professionnelles et des pratiques existantes. Elle vise à professionnaliser les acteurs scolaires dans leur rôle d’orientation, en les dotant de compétences transversales telles que la formulation de feedback réflexif, la mobilisation d’outils numériques et la mise en réseau avec les acteurs du système éducatif. Trois fonctions fondamentales leur sont attribuées : celle d’allié, de catalyseur et de relais, afin de favoriser une orientation contextualisée, continue et inclusive.
En conclusion, le rapport insiste sur la nécessité de penser l’orientation comme une démarche éducative à part entière, sensible à la complexité du monde contemporain (VUCA) et à la singularité des parcours. La plateforme Ada, couplée à une formation structurée et éthique, est présentée comme un levier de transformation pédagogique et d’émancipation pour les jeunes en questionnement sur leur avenir.4. Quality education8. Decent work and economic growth16. Peace, justice and strong institution
Architecture, conseil et enseignement
No full textprésentation d'un profil "métier" reliant architecture, urbanisme et recherche4. Quality educatio
DEFINITION DES ENJEUX DE DURABILITE ET DE D’EFFICACITE D’UN PROJET DE CONSTRUCTION AUJOURD’HUI EN BELGIQUE
Get PDFLes ODD définissent un scope large d’impact environnemental et de responsabilité sociale de l’entreprise via les principes RSE/ESG. Dans cette lignée, l’Europe tient à garantir ces principes. Elle a donc mis en place la taxonomie (DNSH) qui est un outil financier qui définir des objectifs de résultats à partir d’indicateurs. Il reste donc à définir les méthodes pour mesurer et quantifier ces indicateurs (échelle européenne) . Ce sont les pays qui définissent les méthodes.7. Affordable and clean energy11. Sustainable cities and communities13. Climate actio
Voyage initiatique et langue de spécialité : le parcours d’un viking analysé au prisme de la textométrie
Get PDFStructurally Colored Sustainable Sea Silk from Atrina pectinata.
No full textpeer reviewedThe harvesting of sea silk, a luxurious golden textile traditionally obtained from the endangered mollusk Pinna nobilis, faces severe limitations due to conservation efforts, driving the search for sustainable alternatives. Atrina pectinata, a phylogenetically close relative within the Pinnidae family is identified, as a viable source of biomimetic sea silk. The byssal threads of A. pectinata can be processed using existing methods, providing a way to continue producing this historically significant textile. These threads exhibit a remarkable hierarchical structure with globular proteins organized across multiple scales and stabilized by supramolecular sugar-lectin interactions that influence their mechanical properties. Moreover, the threads display a brilliant golden hue arising from structural coloration, ensuring exceptional lightfastness, retaining their color for millennia. This discovery elucidates the biomolecular foundations of sea silk's unique properties and establishes A. pectinata as a sustainable candidate for producing exquisite golden textiles and bioinspired pigments, thereby addressing the growing demand for eco-friendly and long-lasting colored materials in the textile and pigment industries.14. Life below wate
Russia's worldview behind its disinformation campaigns
Get PDFeditorial reviewedSpecializing in in Russian discourse and propaganda, Anne Delizée will bring her socio-linguistic approach to explore how cultural and ideological narratives are crafted to frame the West as a threat to Russian identity
Présence à distance et agents conversationnels : représentation des apprenants dans un dispositif hybride
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Exploring the lived-experience of visually impaired individuals regarding their autonomy, using the example of the community pharmacy: A qualitative study
Get PDFpeer reviewedBackground: Visual impairment constitutes a significant public health concern, affecting a large number of individuals on a global scale. The impact of visual impairment on the daily lives of individuals is considerable, in particular with regard to the administration of medications and interactions with community pharmacists. Community pharmacists occupy a pivotal role as a point of contact for visually impaired patients. The pharmacy is a place of reception and support, and therefore must provide the best possible services to all its patients. Consequently, it is imperative to gain an understanding of the specific needs of visually impaired individuals, who are particularly vulnerable, to facilitate their inclusion in healthcare.
Approach: The objective of this study was twofold: firstly, to examine the perceptions, challenges, and needs of visually impaired individuals in managing their medications; secondly, to enhance pharmacist-patient communication and ensure more accessible and inclusive healthcare for these individuals.
A qualitative study was conducted with French-speaking visually impaired individuals living in Belgium. The participants were recruited voluntarily, and an interview guide comprising six main topics was developed based on a comprehensive literature review. Semi-structured interviews were carried out until theoretical data saturation was reached, recorded, transcribed verbatim, and analysed in a double-blind fashion using abductive thematic and interpretative phenomenological analyses. The data were organised by NVivo 14 software, and a Cronbach's α coefficient was calculated using IBM SPSS 29 Advanced software to verify the reliability of the analyses.
Results: Two dimensions, psychological and pharmaceutical, were explored in the semi-structured interviews. In total, 19 interviews were conducted, and seven themes were identified. The Cronbach's alpha coefficient was 0.938, indicating that the analyses were highly reliable. From a psychological perspective, the experience of individuals with visual impairment appears to be linked to their family and social context. The emotional states of visually impaired individuals and the actions of their family members and friends, who occasionally assume the role of informal caregivers, can be either beneficial or detrimental. The relationship they have with their pharmacist is also important, especially in regard to the relational aspect and the knowledge of visual impairment. During the interviews, visually impaired individuals indicated that pharmacists do not frequently offer additional guidance beyond dosage and storage instructions. They also reported that they devised themselves strategies to facilitate the administration of their medications (color-coding system, touch to identify medications, memory, or assistive products).
Implications: Visual impairment is a complex phenomenon that is influenced by a variety of factors. Consequently, adaptation systems are necessary at all levels of the society (micro, meso, and macro) to provide visually impaired individuals with the best living conditions and people-centred care. The results of this qualitative study offer valuable insights that can inform the development of recommendations for community pharmacists