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Design and evaluation of azobenzene-functionalized macrocyclic peptoids as tunable molecular photoswitches
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Impact of Post-Translational Succination on Collision-Induced Unfolding of SUMO1
No full textpeer reviewedSmall Ubiquitin-like Modifier 1 (SUMO1) is a key protein involved in the post-translational modification (PTM) known as SUMOylation, which regulates essential cellular processes such as protein localization, stability, and complex formation. SUMO1 contains a single free cysteine (Cys52) located in an α-helix, which makes it susceptible to succination, another PTM resulting from Michael addition between a thiol group and fumarate, forming 2-(S-succino)cysteine (2SC, Figure 1). Recent studies have shown that succination induces structural alterations in SUMO1 by disrupting its hydrogen-bond network, potentially altering its biological function leading to metabolic alterations. While SUMO1 and its succinated form naturally operate in solution, ion mobility techniques coupled to mass spectrometry (IMS-MS) such as collision-induced unfolding (CIU), probe their structural properties in the gas phase. CIU provides sensitive fingerprint readouts of protein stability, nevertheless these patterns may be difficult to interpret without complementary approaches. In this study, molecular dynamics (MD) simulations across a range of temperatures, used to mimic experimental activation, were performed to gain deeper insight into the structural changes highlighted by CIU profiles. We also applied Monte Carlo simulations to correlate experimental and theoretical CIU50 through the determination of ion temperature in the drift tube following studies of Prell et al
Preliminary Insights on the AABI-ID: Exploring Caregiver Perspectives on Behavioural Patterns in adults with ID from an Attachment-Based Perspective
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Formaldehyde degradation in ambient air with immobilized Ogataea polymorpha in a hybrid sol-gel coating
Get PDFpeer reviewedFormaldehyde is among the most ubiquitous volatile organic compounds (VOCs) in indoor environments, continuously emitted by construction, insulation, and decorative materials such as paints, vinyl, and carpets. Due to its toxicity, irritant effects, and carcinogenic potential, there is an urgent need for efficient, cost-effective, and sustainable removal strategies of this pollutant. This study investigates a bio-based approach leveraging the enzymatic detoxification capabilities of the thermotolerant methylotrophic yeast Ogataea polymorpha. The yeast was immobilized in a hybrid sol-gel matrix and sprayed onto cellulose paper to create a functional biocoating.
The research focused on optimizing culture conditions, acclimating the strain to formaldehyde concentrations ranging from 1 to 10 mM, and evaluating its degradation performance in aqueous solutions using fresh cultures, free biomass, and immobilized freeze-dried cells. Preliminary gas-phase tests were also conducted to assess the biocoating’s ability to reduce airborne formaldehyde levels emitted by wood wool insulation at ambient temperature.
Results showed that O. polymorpha can grow in media containing up to 10 mM formaldehyde, with a decrease in maximum growth rate (μmax from 1.43 to 0.4 h⁻¹) and an extended lag phase (from 29.9 to 58.9 h). Strains pre-exposed to formaldehyde exhibited enhanced degradation kinetics. The freeze-dried, immobilized yeast effectively degraded aqueous formaldehyde solutions (2–10 mM) within 6 hours. Initial gas-phase experiments yielded promising results, demonstrating the potential of this biocoating to reduce indoor formaldehyde concentrations emitted by recycled MDF-based wood wool insulation at 28°C.9. Industry, innovation and infrastructur
Les impacts fonctionnels des troubles neurodéveloppementaux sur la participation scolaire chez des enfants du primaire ordinaire : Scoping review
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Chitosan-enhanced hydroponic cultivation of Ocimum basilicum L.: a sustainable approach for improved growth, quality and antioxidant activity
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Field-realistic concentrations of copper but not cadmium reduce survival without affecting reproductive traits in Bombus terrestris males.
Get PDFpeer reviewedUnderstanding how environmental pollutants affect male reproductive traits in bees is critical for elucidating mechanisms underlying pollinator decline. While trace metals are increasingly recognised as widespread contaminants, their effects on male reproductive success in bees remain unexplored. Here, we investigated the impact of field-realistic exposure to copper and cadmium, two prevalent trace metals, on reproductive traits in adult Bombus terrestris males. Males were exposed via sucrose solution to copper, cadmium, or a copper-cadmium mixture, and evaluated for survival, competitive mating success, sperm count and sperm viability. Twelve-day exposure to copper and the copper-cadmium mixture significantly increased male mortality (i.e., 82 % and 75 % mortality, respectively), suggesting physiological stress potentially linked to impaired nutrient assimilation, as evidenced by reduced syrup intake. By contrast, cadmium-exposed males exhibited survival (i.e., 15 % mortality) and feeding rates (i.e., 1.65 g of sucrose syrup) comparable to controls and showed no differences in competitive mating success (i.e., 50 % of control and 50 % of cadmium-exposed males succeeded) or mating duration (i.e., ∼29 min for control and cadmium-exposed males). Additionally, six-day exposure to metals did not significantly affect sperm count or viability (i.e., ∼63 % viability in all treatments), though both parameters showed high inter-individual variability. Our results therefore indicate that adult exposure to field-realistic concentrations of copper and cadmium does not impair reproductive traits in bumble bee males, although copper poses a lethal risk. The absence of detectable effects on sperm quality may reflect limited metal accumulation in reproductive tissues, potentially due to sequestration in other organs. As spermatogenesis starts during larval development in bumble bees, we advocate future studies to also evaluate sperm parameters after larval exposure. Additionally, we encourage further research on additional reproductive traits such as pheromone signalling and sperm transfer efficiency
Taming Infinity One Chunk at a Time: Concisely Represented Strategies in One-Counter MDPs
Get PDFpeer reviewed5555 - ASP-Randour - ControlleRS - Fédération Wallonie Bruxelles5727 - PDR-Randour - ControlleRS3284 - CQ-Randour - ManySynth - Fédération Wallonie Bruxelle