Journal of Spices and Aromatic Crops
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Impact of paddy straw mulch on germination and growth of celery (Apium graveolens L.) seedlings and associated weeds in nursery
Full text linkA study was undertaken during three consecutive rabi seasons of 2014–15 to 2016–17 at the Research Farm, Department of Agronomy, Punjab Agricultural University, Ludhiana (Punjab) to determine the effect of paddy straw mulch load and retention time on the germination and growth of celery and associated weeds. The experiment was conducted in randomized complete block design (RCBD) with nine treatments viz., paddy straw mulch @ 4 and 6 t ha-1 with retention time of 15, 20, 25 days after sowing (DAS), full time retention (60 DAS), and control (without mulch). The results indicated that application of paddy straw mulch @ 4 or 6 t ha-1 significantly improved the germination of celery in nursery as compared to no mulch treatment but retention of paddy straw mulch beyond 20 days after sowing suppressed the celery seedlings adversely resulting in lowering seedling population as well as fresh and dry weight of celery seedlings. Application of paddy straw mulch @ 4 or 6 t ha-1 resulted in significantly lower density and dry weight of weeds as compared to control. Further, each successive increase in retention time of paddy straw mulch from 15 DAS to full time retention (60 DAS) through 20 and 25 DAS significantly reduced the weed population in celery nursery
Morphological characterization and secondary metabolites profile of black pepper (Piper nigrum L.) genotypes from Sikkim
Full text linkQuantification of volatile oil and analysis of four major metabolites using HPLC was done in 24 black pepper genotypes collected from south Sikkim. The amount of volatile oil ranged from 2.01% to 0.022%. Secondary metabolites like piperine ranged from 2.75-0.022%, myrcene from 2.094-0.022%, alpha- phellandrene from 1.373-0.008% and linalool from 0.834-0.012%. Genotype 23 had the highest amount of myrcene and linalool, genotype 13 had the highest quantity of piperine and genotype 8 had high amount of alpha-phellandrene. The principal component analysis (PCA) of analyzed metabolites grouped the genotypes into four categories. The study revealed that some of the genotypes were as good as pepper varieties grown in traditional areas. These genotypes will be useful in crop improvement strategies and suitable for Sikkim Himalaya
Volatile metabolites of endophytic Klebsiella aerogenes from Zingiber zerumbet rhizome and its antagonistic effect on soft rot causative Pythium myriotylum
Full text linkRhizomes of Zingiber zerumbet collected from their natural habitat and reported earlier to have high zerumbone content were selected for isolation of endophytes. Biochemical and molecular characterization using 16S rRNA sequencing of the endophytes identified the isolates as belonging to genus Klebsiella, Pantoea and Enterobacter. Isolate designated ZzKSD8 identified as K. aerogenes yielded maximal antagonistic activities against P. myriotylum determined as 83.5% ± 0.77. Volatile metabolites produced by ZzKSD8 caused absolute impairment of P. myriotylum hyphal growth compared to control (23.9 ± 0.37 cm). Volatile metabolites were extracted from 48 hour grown stationary phase ZzKSD8 cultures using absolute methanol, ethyl acetate and ethanol (60% v/v). GC-MS metabolite profiling detected alkanes and fatty acid methyl esters as the predominant constituents in the solvent extracts. Major constituents included methyl palmitate (31.37%), methyl stearate (18.57%) and cyclopropaneoctanoic acid, 2-hexyl-, methyl ester (17.05%) in methanol extract; alkanes like tetratetracontane (13.18%) and 2-methyloctacosane (12.10%) in ethyl acetate extracts and 4,22-sigmastadiene-3-one as major metabolite (22.51%) and stigmast-5-en-3-ol, (3.beta) (17.40%) in ethanol extract. Identified metabolites reported to modulate defense strategies in plants against phytopathogens makes ZzKSD8 a potential candidate for development of biological alternatives to control soil-borne soft-rot disease
Genotype x environment interaction and stability of indigenous coriander (Coriandrum sativum L.) genotypes for seed yield in different agro-climatic zones of Chhattisgarh
Full text linkThe present study was conducted to find out the stability and yield performances of 13 genotypes of indigenous coriander (Coriandrum sativum L.) evaluated in different agro climatic zones of Chhattisgarh. The trials were laid out in a Randomized Block Design (RBD) with three replications at three locations for three years resulting in nine environments (Genotype × year interactions). The genotypes and G × E interactions revealed significant differences at p <0.01 for seed yield indicating varieties and testing environments were distinct from each other. Additive main effects and multiplicative interaction analysis (AMMI-biplot) indicated that the yield performances of indigenous coriander genotypes were highly affected by the environments. The first two principal component axes (PCA 1 and PCA 2) were significant and they explained 67% of the total genotype x environment interaction of which 42.4% and 24.6% were represented by PCA 1 and PCA 2, respectively. A biplot generated using genotypic and environmental scores of the first two AMMI components demonstrated that genotype with larger PCA 1 and lower PCA 2 scores were high yielding and stable genotypes and genotypes with lower PCA 1 and larger PCA 2 scores were low yielding and unstable cultivars in tested locations. The genotype GC 5 C-101 (ICS 4) showed higher grain yields (16.35 q ha-1) over grand mean (13.03 q ha-1) and also had the minimum PCA 1 score, minimum AMMI stability value (ASV) and yield stability index (YSI). Therefore genotype ICS 4 (Chhattisgarh Shri Chandrahasini Dhaniya -2) showed wider stability across different agro climatic environments of Chhattisgarh
DNA barcoding to resolve phylogenetic relationship in Myristica spp.
Full text linkMyristica is the largest and primitive genus of the taxonomically complex family Myristicaceae. DNA barcoding was used to study the evolutionary relationship between Myristica spp. and other genera of Myristicaceae. The barcoding loci namely, rbcL, matK, psbA-trnH, ITS and multilocus combinations were tested to assess their phylogenetic relationship. psbA-trnH locus revealed information regarding the relationship of species in Myristica genus. M. fragrans was found to be closely related to M. beddomei, M. amygdalina, M. andamanica1, whereas M. Fatua was found to be distinct from M. malabarica. Gymnocranthera and Knema species were found to share sister relation with other Myristica spp
Essential oil and thymol of ajwain as effective antioxidant agents
No full textAjwain is an essential herbal plant with many health and medicinal values. Quantitative phytochemical analysis of ajwain was carried out to determine total phenolic compounds, flavonoids, saponins and tannin content. The phytochemical contents were in the order saponins > tannins > flavonoids > phenolics. Essential oil was extracted and chromatographed for the major constituent thymol which was further characterized with spectral techniques. Further, role of essential oil of ajwain seeds and its major compound thymol for antioxidant activity in respect of total antioxidant capacity and free radical scavenging potential were worked out. Out of thymol and ajwain oil, former was found to be more effective antioxidant at all tested concentrations. Inhibition concentrations (IC50) of oil, thymol and ascorbic acid were found to be 1.87, 1.46 and 0.01 mg mL-1, respectively. Results suggest that ajwain and thymol can be used as natural substitutes to synthetic antioxidants in preservation of various food products
Genetic variability, correlation and path analysis in tamarind (Tamarindus indica L.)
Full text linkThe present study was conducted at the Horticultural College and Research Institute, Periyakulam with the objective to estimate the extent of genotypic and phenotypic coefficient of variation, correlation and path analysis among tamarind genotypes. A remarkable variability was observed among the tamarind collections for all the characters. In all the cases, phenotypic variance was higher than the genotypic variance. Also, phenotypic coefficient of variation was found higher than genotypic coefficient of variation for all the traits. The high heritability coupled with high genetic advance as per cent over mean was observed in the traits such as pod yield plant-1 (98.07%; 76.103%), fruit weight (89.11%; 63.668%), fibre weight (89.95%; 91.967%), shell weight (86.19%; 58.534%) and pulp weight (74.13%; 51.533%) whereas the lowest values were recorded for pod length (34.91%; 13.945%) and tree circumference (20.34%; 8.198%). Thus, it indicated better scope for improvement of these traits through selection programme. Pod yield tree-1 was significantly and positively correlated with pod width, tree circumference and pulp weight. Path coefficient analysis showed that pod yield tree-1 contributed the maximum positive direct effect
Genetic diversity studies of cumin (Cuminun cyminum L.) genotypes in western plains of Rajasthan
Full text linkGenetic diversity was studied on fifty-four genotypes of cumin, Cuminum cyminum L. at “Agricultural Research Station, Mandor, Jodhpur” during rabi season 2017-18. The mean squares were found significantly different for all the characters under study, depicting the availability of variability among the study materials. The high magnitude of phenotypic coefficient of variance (PCV) and genotypic coefficient of variance (GCV) for seed yield, primary branches per plant and number of umbels per plant depicted the presence of vast amount of variation for the character along with high heritability (68-97%) combined with higher genetic advances as percentage of means for seed yield. The highest intra-cluster distance was recorded in cluster VI (11.8) along with cluster VII (11.33) and cluster VIII (8.29) depicting large genetic variability among the genotypes of these three clusters. The highest inter-cluster distance was reported among cluster III and VIII (51.97) followed by cluster III and VII (40.07) and cluster IV and cluster VIII (34.77), suggesting wide range of diversity between genotypes of the clusters. Amongst the characters, seed yield contributed the highest towards genetic divergence (47.80%) followed by number of umbel per plant (25.65%), branches per plant (8.60%) and 1000 seed weight (6.64%)
Efficacy of bioinoculants on biomass, nutritional status and yield of lemon grass (Cymbopogon citratus (DC.) Stapf.).
Full text linkIn the present investigation, different bioinoculants were analysed to test their efficacy as bio-fertilizer value for medicinal plant i.e. Lemongrass (Cymbopogon citratus (DC.) Stapf.) under controlled conditions in a poly house. Bioinoculants including dominant Arbuscular Mycorhizal Fungi (Glomus mosseae and Acaulospora laevis) and Phosphate solubilizing Bacteria (Pseudomonas fluorescens) were taken in present experimentation. The independent roles of these bioinoculants or with different combinations were assessed on one month old saplings of C. citratus in pot conditions.The results showed that application of these bio-inoculants were very effective for growth responses, biomass, biochemical, yield parameters. A consortium of G. mosseae, A. laevis and P. fluorescens showed best results for plant height, root length and in increasing the biomass. Mycorrhization status was recovered highest in the consortium treatment. The biochemical and yield (biomass) parameters were also better with consortium. The findings of the present study are informative for herbalist, to use these efficient bioinoculants as bio-fertilizer for the better yield of C. citratus along with least adverse effects on microbial diversity and stability of soil as compared to commercially used inorganic fertilizer
Plant growth promoting rhizobacteria as a biological tool for augmenting productivity and controlling disease in agriculturally important crop- A review
Full text linkThe ecological role of plant growth promoting bacteria associated with plant root environment is currently gaining increased attention. The tremendous use of chemical fertilizers and pesticides which are environmentally harmful can be replaced with these microbes generally called as Plant Growth Promoting Rhizobacteria (PGPR). These microbes can be developed as biofertilizers because they are eco-friendly, cost effective and are natural living organisms. The advantages of these microorganism rely on their production of novel bioactive metabolites that inhibit various pathogens in soil as well as their vast mechanisms in improving growth, productivity and yield in agriculturally important crops. Apart from this, their role in nutrient acquisition, soil fertility improvement and stress tolerance are also inevitable. This review represents the present scenario of beneficial bacteria as plant growth agents and disease control tools. They have been extensively studied for plant disease suppression, plant growth modulation, and interaction with plants. Here, some mechanisms employed by plant growth promoting bacteria from different environments are discussed