Asian Food Science Journal
Not a member yet
778 research outputs found
Sort by
Antibacterial Activity of Synbiotic Yoghurt Peel Extract of Red Dragon Fruit (Hylocereus polyrhizus) Evaporation with Honey
Synbiotic yogurt contains both probiotics and prebiotics. The peel of red dragon fruit contains 10.79% pectin, which can be used as a prebiotic. Red dragon fruit peel synbiotic yoghurt has an unpleasant aftertaste. Honey can enhance the functional properties of dragon fruit peel synbiotic yogurt. Yogurt with the addition of prebiotics can increased the functional properties of probiotic yogurt and increase the viability of bacteria the digestive tract. The purpose of this study was to determine the antibacterial activity of synbiotic yogurt with evaporated red dragon fruit peel extract and honey. This research was conducted at the Laboratory of Animal Products Technology, Faculty of Animal Husbandry, Universitas Brawijaya. The research material is 3-month-old male 15 white rats (Rattus norvegicus) weighing between 150-180 g and divided into 3 groups, probiotic yoghurt, synbiotic yoghurt with addition of red dragon fruit peel extract (Hylocereus polyrhizus) 20% made from 10% skim milk and yoghurt starter containing Lactobacillus bulgaricus and Streptococcus thermophilus (1:1), and the addition of 2% honey to synbiotic. Animals were given probiotic yogurt (P0), 20% red dragon fruit peel extract synbiotic yogurt (P1), and 20% red dragon fruit peel extract synbiotic yogurt with 2% honey sweetener (P2). The duration of experimenting is 30 days. Afterward, white rats will be dissected and the faeces in the ileum will be taken for testing. The data obtained were analyzed using descriptive qualitative. The results showed that P0 did decrease coliform and Enterococcus spp but increase total bacteria, mold and yeast, and Escherichia coli. The addition of evaporated red dragon fruit peel extract in synbiotic yogurt (P1) did decrease total bacteria, coliform, and Enterococcus spp but increase in mold and yeast and Escherichia coli. The addition of honey (P2) did decrease total decrease, mold and yeast, and Enterococcus spp but increase Escherichia coli. The addition of honey to the synbiotic yogurt of red dragon fruit peel extract can affect the total number of bacteria, mold and yeast, coliform, Escherichia coli, and Enterococcus spp
Physical Characteristics of the Development of Edible Film Gelatin-Xylitol Using Butterfly Pea Extract as an Antioxidant
The study aims to determine a gelatin-xylitol edible film with the addition of butterfly pea extract (Clitoria ternatea L) using the Microwave Assisted Extraction method with optimal physical characteristics of the edible film. The research method uses the experimental method with calculations using a Completely Randomized Design (CRD), followed by Duncan analysis (DMRT) if there are significant or very significant effects in each treatment. This research method focuses on differences in the percentage of butterfly pea extract in the manufacture of gelatin-xylitol edible films with 5 treatments P0 (0%), P1 (1%), P2 (2%) P3 (3%), and P4 (4%) and 3 repetitions. The results of the second phase of the study showed the effect of different butterfly pea extracts on edible gelatin-xylitol. There was no significant effect (P>0.05) in the sedimentation test, L and a color, there was a significant effect (P<0.05) in the b color test analysis and in the antioxidant test analysis. The functional groups identified in the eggplant extract were alkanes, alkenes, fluorides, and aromatics. The analysis of the Scanning Electron Microscope test showed the presence of particles in the edible film was 3% addition of butterfly pea extract produces an edible film that has antioxidants
Effect of Some Preservatives on the Microbial Quality of Cold-smoked Catfish at Refrigerated Temperature
Aim: This study investigated the preservative effect of potassium sorbate, ginger, garlic, and a blend of, ginger and garlic on the microbiological quality and shelf stability of cold smoked Catfish (Clarius gariepinus) at refrigerated storage.
Methodology: One hundred (100) of the freshly harvested catfish obtained from the MOUAU Fish Farm were processed and divided into five lots of 20 each. Four marinating baths containing 0.5% potassium sorbate, 3% ginger, 3% garlic, and a blend of 1.5% ginger and 1.5% garlic respectively were prepared. Each catfish lot except the fifth lot (which served as a control) was marinated for 2 h and cold smoked for 8 h. The samples were then stored for fourteen days at refrigeration temperature (4 ± 2 °C during which analyses were carried out. The Total viable count, Total fungal count, and bacterial counts (Escherichia coli, Salmonella, Staphylococcus sp, and Listeria sp) of the catfish samples before, during, and after storage were analyzed.
Results: The total viable count (TVC) was reduced from 8.7x105 to 1.2x105 CFU/g, 1.13 x106 to 2.4x105 CFU/g, 1.09 x 106 to 2.00 x105 CFU/g, 9.60 x105 to 1.50 x105 CFU/g, and 1.19x106 to 3.00x105 CFU/g for 3% ginger, 3% garlic, 1.5% ginger +1.5% garlic, 0.5% potassium sorbate, and the control at refrigeration storage respectively. All the treatments were effective against E. coli, Listeria monocytogen, and Salmonella count. Similarly, the 3% of ginger treatment recorded counts of 1x105 CFU/g for Staphylococcus counts, during refrigeration storage. Furthermore, the total fungal count decreased for all the treatments.
Conclusion: The study found that cold smoked catfish treated with 0.5% potassium sorbate, 3% ginger, 3% garlic and a blend of 1.5% ginger and 1.5% garlic can be stored for 14 days at refrigeration storage provided the storage condition is maintained
Characteristics of Seasoning Powders from Nagara Bean Tempeh and Oyster Mushroom for Umami Enhancer to Reduce Salt-use
Aims: Nagara bean tempeh and oyster mushroom have high protein, and free glutamate can increase the umami effect. The formulation is needed to obtain the desired umami level.The research aimed to formulate seasoning powders of Nagara bean tempeh flour, white oyster mushroom flour, salt, and sugarto enhance the umami taste.
Study Design: This research formulated seasoning powder using a randomized block design involving two treatment factors: 1) the concentration of salt, Nagarabean tempeh flour, and white oyster mushroom flour,and 2) the percentage of sugar.
Place and Duration of Study: Laboratory of analytical chemistry and industrial environment, Department of Agroindustrial Technology, Faculty of Agriculture, LambungMangkurat University, between June and November 2021.
Methodology: The seasoning flavor formulation includes the proportion of salt 50% to 20 %, and the remaining composition is the ratio of Nagara bean tempeh flour and oyster mushroom flour 50: 50. The addition of sugar 0%, 25%, and 30% is also studied in this formulation to determine the effect of strengthening the umami taste. The seasoning powder was analyzed by chemical and sensory analysis.
Results: Data analysis showed that the NaCl content in the seasoning powder decreased according to the reduced salt concentration in the seasoning formula. The proportions of salt: Nagara bean tempeh flour and oyster mushroom flour 20: 40: 40 produced the highest soluble protein content, total acid, and insoluble solids. Adding 25-30% sugar strengthened umami sensory test results more than the others. The results of salty taste scoring correlated with the proportion of salt in the formulation, where the salt ratio of 40%-50% was significantly not different.
Conclusion: Seasoning powder formulation using salt, Nagara bean tempeh flour, and oyster mushroom flour did not yet provide a strong umami effect. The umami effect should be enhanced by added sugar. Seasoning powder with slightly strong umami was obtained in the formulation of salt: Nagara bean tempeh flour: oyster mushroom flour by 20: 40: 40 with the sugar addition of 25%. This formula, reduced salt use in seasoning
Effect of Modifications on the Chemical Composition, X-Ray Diffraction Pattern and Scanning Morphology of Starches from Cocoyam (Colocasia esculenta and Xanthosoma sagittifolium)
The effect of annealing, heat moisture treatment and citric acid on major physicochemical properties of cocoyam starch was investigated. This was done in order to establish the optimum processing condition with the aim of enhancing the utilization capacity of the starch. Starch extracted from red and white Cocoyam (Tania) Xanthosoma sagittifolium and Taro Colocasia esculenta cultivars respectively were subjected to modification using annealing, heat moisture treatment and citric acid methods. The modified and native starch samples were analyzed for chemical composition using standard methods, likewise the x-ray diffraction pattern and starch granules morphology of the samples were assessed. The result showed that amylose content ranged from 24.81 - 38.16%, protein 0.00 – 0.77%, ash 1.19 – 3.16 and carbohydrate 84.19 – 86.79%. The X-ray diffraction patterns of red and white cocoyam starches showed a strong peak at 15o, 18o and 23o (2ø) indicating A-type starch. Colocasia esculenta starch also displayed A-type diffraction pattern with additional peak at 24o. The modification methods used did not change the diffraction pattern of the starch samples but only influenced the intensity of the diffraction peak. The scanning electron micrographs showed that the starch granules were small to medium in size with most having irregular shape and smooth surface. Annealing and citric acid caused little noticeable fractures on some granules without compromising starch granules integrity. Heat moisture treatment however resulted in evident loss of starch granules integrity. The modification methods affected the physicochemical properties of cocoyam starch samples in a varying pattern which may qualify the starch samples for use in different food and non-food applications
Response Surface Methodology Approach for the Production of Enriched Rice-based Breakfast Cereal
This study was aimed at the use of blend of rice flour (Oryza sativa), pigeon pea flour (Cajanus cajan), and banana flour (Musa paradisiaca) to produce enriched rice-based breakfast cereal. The control was done without blending the three flours (100% pre-gelatinized rice flour). The proximate composition was evaluated according to standard methods. The proximate composition showed that moisture, ash, fat, protein, carbohydrate and fibre content in % ranged from 5.66-7.95, 0.40-2.51, 0.50-1.51, 2.78-11.21, 79.12-89-62 and 0.10-1.42 respectively. Enrichment of rice-based breakfast cereals could contribute to improvement of nutritional status if adopted as a nutritious, healthy indigenous breakfast cereal
Physico-chemical Characteristics and the Effects of Processing Methods on the Nutritional and Anti-nutritional Quality of Soybean (Glycine max (L.) Merrill)
The present investigation was carried out to determine the physico-chemical characteristics and effects of processing methods on the nutritional and anti-nutritional quality of soybeans. Soybean seeds were analysed using physico-chemical approaches, in order to obtain a detailed profile on these qualities. The effects of processing methods on the nutritional and anti-nutritional quality of soybean had an impact on the nutritional and anti-nutritional values. Soybeans were subjected to different processing methods which were sorted, washed, dried, and milled into flour (Sample A- Control), soaked in water (1:3) for 12h, mechanically dried at 60 ± 2 oC for 10 h and milled into flour (Sample B), soaked in water (1:3) for 12h, sprouted 72 h, mechanically-dried at 60 ± 2 oC for 10 h, roasted for 3 min and milled into flour (Sample C), boiled for 30 min in an open lid, mechanically-dried at 60 ± 2 oC for 10 h, roasted for 3 min and milled into flour (Sample D). The study revealed that raw soybean seeds had 90.43 ±3.93 g seed weight, 6.31 ± 0.41 mm length, 5.50 ± 0.29 mm breadth,4.58 ± 0.35 mm height, 0.88 ± 0.04 mm sphericity, 753.01 ± 6.62 kg/ m3 bulk density, 1181.03 ± 11.72 kg/ m3 true density, 36.33 ± 0.79 % porosity, 0.13 ± 0.005 g/seed hydration capacity, 1.25 ± 0.07 hydration index, 0.12 ± 0.01 mL swelling capacity, 1.62 ± 0.05 swelling index. The colour was recorded to be (L* 59.52, a* 8.97, b* 34.01), Chroma (c*) 35.17, and hue angle (ho) 9.84. After processing, moisture content increased (10.61 - 12.43 %), crude protein (42.97– 47.87 %), crude fibre (9.44 - 11.57 %), ascorbic acid (5.38 - 11.65 mg/ 100 g), and ash content (4.84-5.99 %) while total carbohydrates and total energy decreased significantly (17.70 - 15.92 %) and (439.69 - 397.82 %), respectively. Similarly, the phytic acid content decreased from 8.12 to 5.19 mg/100 g while the tannin decreased from 25.34 to 18.57 % and the protease inhibitor decreased from 7.12 to 5.01 %. The overall results of the current study revealed that the processing methods of soybeans had an impact on the nutritional and anti-nutritional values. Further, the study showed that processing methods can significantly improve the nutritional qualities of soybeans while substantially reducing their anti-nutritional properties, thereby boosting the nutrients' bioavailability
Implementation of Good Manufacturing Practices in Milk Processing Companies in Nairobi County and Microbial Contamination of Milk and Milk Products
Good manufacturing practice involves a series of measures to be adopted by the food industries in order to guarantee the safety and conformity of food products to specific regulations. Even though good manufacturing practices are vital systems in food safety and is associated with minimum sanitary and processing requirements for the food industry, only a few studies have reported GMP implementation by small milk processing companies. Therefore, the present study was undertaken in milk processing firms to evaluate the implementation of good manufacturing practices for control of microbial contamination in milk and milk products. Purposive, random and stratified sampling techniques was used to identify milk processing companies. Ten processing facilities were purposively sampled for the study. The representatives were interviewed, using a pretested questionnaire and data was collected and analyzed. All the processors had qualified personnel handling milk and milk products with a daily processing capacity of 20000-30000 litres. It was observed that the processors complied with all regulatory and licensing requirements and had in place critical process controls with majority indicating pasteurization at temperatures ranging from 80-90oC as the most common method. The processors had several food safety management systems such as ISO 22000, GMPs, and HACCP which were handled by trained and competent staff. It was observed that all the processors tested for total viable counts (TVC), and E. coli while only 50% and 33.3% of the processors tested for S. aureus and L. monocytogenes respectively. Furthermore, the processors (33.3%) reported E. coli as the main contaminant while L. monocytogenes were not detected. A majority (83.3%) had well-documented cleaning programs and had a system of controlling cross contamination which was enforced through different colour codes (66.7%), memos and notices (16.7%) and through colour coding of processing equipment (16.7%). In conclusion, the present study discovered that milk processors had implemented good manufacturing practices (GMP) and conformed to good processing practices
Prevalence of Common Microbiological Pathogen Contamination in Processed Milk and Milk Products in Nairobi County, Kenya
Milk has an outstanding nutritional quality but it is also an excellent medium for bacterial growth and an important source of bacterial infection when consumed without pasteurization. This study aimed at establishing the prevalence of Total Viable Count (TVC), Escherichia coli, Staphylococcus aureus and Listeria monocytogenes contamination on processed milk and milk products. The study was carried out in Karen, Kibera and Langata Sub- Counties of Nairobi County which were purposively chosen because they have glaring contrasts in living standards. Samples of fresh milk, yoghurt cheese and ice creams were collected from supermarkets and prepared for analysis of microorganisms. All isolates were characterized and identified based on their morphological and cultural characteristics. TVC were detected in 100% of the samples collected and there was significant statistical variation (P ≤ 0.05) in the contamination level among the products. Of the samples collected in Karen, ice cream had the highest contamination level (3.26 log10 CFU ml-1). Ice cream samples from Langata had the highest TVC contamination levels at 4.35 log10 CFU ml-1. The overall prevalence of E. coli in milk and milk products was 41.6% with a mean count of 0.34 log10 CFU ml-1 in Karen, 0.07 log 10 CFU ml -1 in Kibera and 0.11 log 10 CFU ml -1 in Langata while Staphylococcus aureus was detected in 33.3% of the milk and milk products. The occurrence and detection of E. coli and S. aureus foodborne pathogens in milk and milk products represent a health risk to consumers. Therefore, there is need to improve the microbial quality of milk and milk products by employing measures that will establish proper management practices to ensure improved hygiene, good manufacturing practices and food systems that will help to minimize microbial contamination
Microbiological and Physicochemical Evaluation of Pineapple Fruit and Its Juice for Compliance with Export Requirements
Fruit juices are unfermented but probably fermentable fruit extracts, stored fresh and consumed around the world due to their great nutritional importance. The objective of this study wasto determine the pesticide residues, the physicochemical and nutritional parameters of pineapple juice and pineapple derivatives in Benin. The samples were taken from pineapple juice producers and pineapple producers. These samples were subjected to microbiological and physicochemical analyzes in the laboratory.
The results show that the average pH value of whole pineapple is 4.1228±0.0130. While and the average pH value of pineapple juice is 4.0762±0.0222. Whole pineapple samples had an average titratable acidity of 85.78±2.27. The pineapple juice samples had an average titratable acidity of 85.73±4.52. Imported whole pineapple samples were found to have the highest average concentration compared to pineapple juice (0.60±0.009 g/L). The average etephon concentration is higher in whole pineapple samples (0.76±0.05) than in pineapple juice samples (0.49±0.06). The average logarithmic contamination rate on whole pineapple samples is around 2.329 log10 CFU/g for FAMT, 0.18 log10 CFU/g for yeasts and molds and 0.014 log10 CFU/g for Staphylococcus aureus. Nevertheless, the average logarithmic contamination rate on whole pineapple samples is less than 10 log10 CFU/g respectively for Coliforms, Faecal Coliforms and E coli. The suspicion of salmonella was around 0% for whole pineapple samples.
While the average logarithmic contamination rate on pineapple juice samples is around 0.71 log10 CFU/ml for FAMT. Nevertheless, the average logarithmic contamination rate on pineapple juice samples is less than 10 log10 CFU/ml respectively for Coliforms, Faecal Coliforms, E coli, yeasts and molds and Staphylococcus aureus. The suspicion of salmonella is around 0% for the pineapple juice samples.
This study reveals that pineapple juice and pineapple derivatives contained various microflora, nutritional element and pesticide residues. It also notes that the microbiological and nutritional quality of pineapple juice and pineapple derivatives varies according to the producers and depends on the hygienic conditions of production