47325 research outputs found
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U. S. Representative Charles Bennett and Dr. Warren Shell
No full textPhotograph: U. S. Representative Charles Bennett, Dr. Warren Shell and unidentified man. Undated.https://digitalcommons.unf.edu/eartha_images/1491/thumbnail.jp
Mechanism of strand displacement DNA synthesis by the coordinated activities of human mitochondrial DNA polymerase and SSB
No full textMany replicative DNA polymerases couple DNA replication and unwinding activities to perform strand displacement DNA synthesis, a critical ability for DNA metabolism. Strand displacement is tightly regulated by partner proteins, such as single-stranded DNA (ssDNA) binding proteins (SSBs) by a poorly understood mechanism. Here, we use single-molecule optical tweezers and biochemical assays to elucidate the molecular mechanism of strand displacement DNA synthesis by the human mitochondrial DNA polymerase, Polγ, and its modulation by cognate and noncognate SSBs. We show that Polγ exhibits a robust DNA unwinding mechanism, which entails lowering the energy barrier for unwinding of the first base pair of the DNA fork junction, by ∼55%. However, the polymerase cannot prevent the reannealing of the parental strands efficiently, which limits by ∼30-fold its strand displacement activity. We demonstrate that SSBs stimulate the Polγ strand displacement activity through several mechanisms. SSB binding energy to ssDNA additionally increases the destabilization energy at the DNA junction, by ∼25%. Furthermore, SSB interactions with the displaced ssDNA reduce the DNA fork reannealing pressure on Polγ, in turn promoting the productive polymerization state by ∼3-fold. These stimulatory effects are enhanced by species-specific functional interactions and have significant implications in the replication of the human mitochondrial DNA
Unidentified People Outside Theater
No full textPhotograph: Three unidentified women and a child in doorway.. Undated.https://digitalcommons.unf.edu/eartha_images/1511/thumbnail.jp
Unidentified Woman with Dog
No full textPhotograph: Woman standing outside with a dog. Undated.https://digitalcommons.unf.edu/eartha_images/1502/thumbnail.jp
Fall 2021 Project InTersect Showcase Program
Get PDFThis program highlights the inquiry presentations of our Inservice and Preservice teachers. The teachers came together to celebrate their STEM+C learning and share lessons learned from implementation. This showcase was a partnership between University of North Florida and Duval County Public Schools.https://digitalcommons.unf.edu/project_intersect_materials/1001/thumbnail.jp
Miss American Beach Beauty Pageant Contestants
No full textPhotograph: Miss American Beach beauty pageant contestants on beach. Undated.https://digitalcommons.unf.edu/eartha_images/1152/thumbnail.jp
Services in the New Dining Room
No full textPhotograph: Services in the new dining room at Old Folks Home. Undated.https://digitalcommons.unf.edu/eartha_images/1188/thumbnail.jp
Residents, Eartha White, and Rev.C.J. Dorrah, Old Folks Home
No full textPhotograph: Rev. C. J. Dorrah, the matron, and Eartha White (front row, second from right) with residents on the steps of the Old Folks Home. Handwritten on reverse: Rev.C. J. Dorrah the matron and E. White out at the Old Folks Home. Undated.https://digitalcommons.unf.edu/eartha_images/1224/thumbnail.jp
John Rosamond Johnson
No full textPhotograph: Portrait of John Rosamond Johnson. On reverse: Diamond Photo Co. 132 Boylston Street Boston. Undated.https://digitalcommons.unf.edu/eartha_images/1211/thumbnail.jp
Gathering at Old Folks Home
No full textPhotograph: Large group of people outside at the Old Folks Home. Eartha White, right foreground. Undated.https://digitalcommons.unf.edu/eartha_images/1185/thumbnail.jp