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'Getting Better All the Time: Hokusai, Beyond the Great Wave'
Get PDFReview of *Hokusai: Beyond the Great Wave* at the British Museum, and Timothy Clark, editor. *Hokusai: Beyond the Great Wave*
Peroxisome motility measurement and quantification assay
Get PDFOrganelle movement, distribution and interaction contribute to the organisation of the eukaryotic cell. Peroxisomes are multifunctional organelles which contribute to cellular lipid metabolism and ROS homeostasis. They distribute uniformly in mammalian cells and move along microtubules via kinesin and dynein motors. Their metabolic cooperation with mitochondria and the endoplasmic reticulum (ER) is essential for the β-oxidation of fatty acids and the synthesis of myelin lipids and polyunsaturated fatty acids. A key assay to assess peroxisome motility in mammalian cells is the expression of a fluorescent fusion protein with a peroxisomal targeting signal (e.g., GFP-PTS1), which targets the peroxisomal matrix and allows live-cell imaging of peroxisomes. Here, we first present a protocol for the transfection of cultured mammalian cells with the peroxisomal marker EGFP-SKL to observe peroxisomes in living cells. This approach has revealed different motile behaviour of peroxisomes and novel insight into peroxisomal membrane dynamics (Rapp et al., 1996; Wiemer et al., 1997; Schrader et al., 2000). We then present a protocol which combines the live-cell approach with peroxisome motility measurements and quantification of peroxisome dynamics in mammalian cells. More recently, we used this approach to demonstrate that peroxisome motility and displacement is increased when a molecular tether, which associates peroxisomes with the ER, is lost (Costello et al., 2017b). Silencing of the peroxisomal acyl-CoA binding domain protein ACBD5, which interacts with ER-localised VAPB, increased peroxisome movement in skin fibroblasts, indicating that membrane contact sites can modulate organelle distribution and motility. The protocols described can be adapted to other cell types and organelles to measure and quantify organelle movement under different experimental conditions
Strategic Approaches to Cloud Computing
No full textCloud-based services provide a number of benefits in areas such as scalability, flexibility, availability and productivity for any organisation. These benefits can be further enriched by considering opportunities, which will allow for organisational enrichment at a strategic level. It is important to align businesses, ICT and security strategies to enable more beneficial outputs overall. Moving to cloud computing needs to consider strategic objectives of businesses essentially how IT cost impacts on the needs of future developments for businesses and IT departments within large organisations. Strategically implementing a cloud strategy can also be considered as a disruptive technology. Disruptive technology can be considered favourable in terms of offering organisational benefits. The most immediate benefits are consistent with reducing cost technology ownership, communication time therefore increasing the time benefits thus allowing organisations to become more productive
Democratization and inequality: Empirical evidence for the Organisation for Economic Co-operation and Development member countries
Get PDFThe correlation between democracy and income inequality, as well as the direction of causation is still debated. In this paper, we conduct a dynamic panel data analysis for all 34 Organisation for Economic Co-operation and Development member states over the period 1960–2012 and illustrate a nonmonotonic relationship between inequality and democracy, as well as test the direction of causation by employing a noncausal homogeneity test in a panel Granger framework and the Dumitrescu and Hurlin noncausal heterogeneity test. We provide an explanation for the inverted U-shaped relation. In addition, we illustrate individual regression results for 12 exemplary Organisation for Economic Co-operation and Development countries and provide a concise illustration of the economic and political changes to which countries have been subjected while elaborating the effect of the underlying policies on democracy and income equality
- ‘Political Conflict and Political Ideas in Twelfth-Century Denmark’, Viking Age and Medieval Scandinavia 13 (2017), 61-100.
Get PDFThis article investigates the nature of political life and conflict in medieval Denmark, focussing on the case of the rebellion against King Niels between 1131 and 1135. The article engages with previous scholarship that has identified the basis of the rebellion, and the governing feature of political life in the period, as the material interests of the competing kin-networks. Through an investigation of both the documentary and narrative sources for the conflict, the reigns of King Niels and his successor Erik II Emune, the leader of the rebellion, this article argues that in fact political and religious principles were much more important. Building on this it argues that we need to pay much more attention to the stated principles of political actors as found in the contemporary sources and the way these enabled aristocrats and would-be kings to mobilise support
Financial crises and plurilateral discourses
Get PDFThis paper analyses impacts of the Eurozone crisis on the global standing of the European Union (EU) through the failure of the EU interregional approach in the EU-ASEAN plurilateral discourse. It appropriates theoretical models of socialisation, in order to explain the dearth of cooperation in the EU-ASEAN plurilateral partnership during the Eurozone crisis. The paper draws on comparative accounts of financial crises and exit strategies from the EU and the Association of South-East Asian Nations (ASEAN). It further utilises the systemic hypothesis from the work of Agarwal and Fogarty on interregionalism (2003) and the appropriateness hypothesis of Schimmelfennig and Sedelmeier (2004), in order to advance two propositions: (i) democratization is an unlikely avenue of EU ‘externalization’ in the EU-ASEAN plurilateral partnership; and (ii) preventing financial crises is the likeliest avenue for lesson-drawing in the EU-ASEAN plurilateral partnership but only if lesson- drawing can go beyond the scope of EU processes and policies, i.e. where the EU can itself be influenced by broader tendencies and patterns in the international system (Schimmelfenning 2012). Failure to see beyond the normative ontological quality of the EU, is likely to diminish the global appeal of the supranational integrative model of the EU and may re-enforce instead the preference for bilateralism over interregionalism in the EU-ASEAN partnership
Big Data and the Well-Being of Women and Girls Applications on the Social Scientific Frontier
Get PDFConventional forms of data—household surveys, national economic accounts, institutional records, and so on—struggle to capture detailed information on the lives of women and girls. The many forms of big data, from geospatial information to digital transaction logs to records of internet activity, can help close the global gender data gap. This report profiles several big data projects that quantify the economic, social, and health status of women and girls
Study of the cap structure of (3,3), (4,4) and (5,5)-SWCNTs: Application of the sphere-in-contact model
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La reparación en la interacción oral de estudiantes de ELE: comparación entre interacciones de práctica en el aula e interacciones en contextos de evaluación
Get PDFBased on the concept of interactional competence (Hellermann, 2008;
Seedhouse, 2004; Walsh, 2011; Young, 2008), our study analyzes how learners of
Spanish as a foreign language in a B2 level manage repair in oral interaction in
language classrooms. We understand repair as “the treatment of trouble in talk-ininteraction”
(Young, 2008, p. 49). A corpus of eleven interactions between students
in the classroom is analyzed through the perspective of Conversation
Analysis. The interactions were collected in different kinds of tasks in the language
classroom. In our analysis we compare interactions produced in practice
activities and interactions collected during assessment. The findings in this study
show a tendency to manage repair in classroom oral interaction as it would be
done in normal conversation. Regarding the different contexts of our corpus, we
observe that, in interactions produced in assessment contexts, speakers try to
protect their interlocutor’s face and their own face by avoiding to make repairs