GRO.publications
Not a member yet
137257 research outputs found
Sort by
Detecting the footprint of selection on the genome of Girgentana goat, a popular ancient breed
No full texthttp://dx.doi.org/10.13039/501100004913 University of Palerm
Subjective cognitive decline in conjunction with cerebrospinal fluid anti-ATP1A3 autoantibodies and a low amyloid β 1–42/1–40 ratio: Report and literature review
No full texthttp://dx.doi.org/10.13039/501100003385 Georg-August-Universität Göttingenhttp://dx.doi.org/10.13039/100007689 Universidade de Aveir
A cross-generational methylomic signature of infant maltreatment in newborn rhesus macaques
No full textMeasuring the ATLAS ITk pixel detector material via multiple scattering of positrons at the CERN PS
No full textAbstract The ITk is a new silicon tracker for the ATLAS experiment designed to increase detector resolution, readout capacity, and radiation hardness, in preparation for the larger number of simultaneous proton–proton interactions at the High Luminosity LHC. This paper presents the first direct measurement of the material budget of an ATLAS ITk pixel module, performed at a testbeam at the CERN Proton Synchrotron via the multiple scattering of low energy positrons within the module volume. Using a four plane telescope of thin monolithic pixel detectors from the MALTA Collaboration, scattering datasets were recorded at a beam energy of 1.2 GeV . Kink angle distributions were extracted from tracks derived with and without information from the ITk pixel module, and were fit to extract the RMS scattering angle, which was converted to a fractional radiation length x / X 0 . The average x / X 0 across the module was measured as [ 0.89 ± 0.01 (resolution) ± 0.01 (subtraction) ± 0.08 (beam momentum band) ] % , which agrees within uncertainties with an estimate of 0.88 % derived from material component expectations
Measurements of WH and ZH production with Higgs boson decays into bottom quarks and direct constraints on the charm Yukawa coupling in 13 TeV pp collisions with the ATLAS detector
No full textAbstract A study of the Higgs boson decaying into bottom quarks (H → b b ¯ ) and charm quarks (H → c c ¯ ) is performed, in the associated production channel of the Higgs boson with a W or Z boson, using 140 fb−1 of proton-proton collision data at s = 13 TeV collected by the ATLAS detector. The individual production of WH and ZH with H → b b ¯ is established with observed (expected) significances of 5.3 (5.5) and 4.9 (5.6) standard deviations, respectively. Differential cross-section measurements of the gauge boson transverse momentum within the simplified template cross-section framework are performed in a total of 13 kinematical fiducial regions. The search for the H → c c ¯ decay yields an observed (expected) upper limit at 95% confidence level of 11.5 (10.6) times the Standard Model prediction. The results are also used to set constraints on the charm coupling modifier, resulting in |κc| < 4.2 at 95% confidence level. Combining the H → b b ¯ and H → c c ¯ measurements constrains the absolute value of the ratio of Higgs-charm and Higgs-bottom coupling modifiers (|κc/κb|) to be less than 3.6 at 95% confidence level
Sexually dimorphic gene expression responses of bovine embryos to the maternal microenvironment on day 13 of gestation
No full textAbstract Background Various studies have highlighted significant differences in developmental kinetics and sensitivity to developmental conditions between male and female bovine embryos. These differences are thought to be caused in part by the sexually dimorphic expression of genes located on the sex or autosomal chromosomes. However, little is known about the dimorphic gene expression patterns of bovine embryos at the initiation of elongation, which is one of the critical stages of development. Furthermore, to the best of our knowledge, there is little or no data available on the sexually dimorphic gene expression patterns in bovine embryos in relation to maternal environmental conditions during the initiation of elongation. Therefore, the main objective of this study was to investigate the sexually dimorphic gene expression responses of embryos to the maternal environment at the initiation of elongation in embryos developed in lactating dairy cows and nonlactating nulliparous heifers. Results Gene expression analysis showed that 159 genes including those involved in steroid biosynthesis and gastrulation were differentially expressed exclusively between male and female embryos developed in cows. Among these, 61 genes including CYP39 A1, CYP2R1 and CYP27B1 were upregulated and 98 genes including HSD17B1, HSD17B10 and aromatase (CYP19 A1) were downregulated in male embryos. Chromosomal analysis showed that 31.2% of the differentially expressed genes (DEGs) including glucose-6-phosphate dehydrogenase (G6PD) were located on the X chromosome, and 96% of those were upregulated in female embryos. Similarly, 254 genes including those involved in female sex differentiation, placenta development, transmembrane transport, and cell adhesion were differentially expressed exclusively between the male and female embryos developed in heifers. Of these, 108 genes including HSD17B11, HSD17B12, and HSD3B1 were upregulated, and 146 genes including SLC16 A9, SLC10 A1, SLC10 A3, SLC16 A5, SLC22 A23, SLC25 A43, SLC35 A2, SLC35 C1, and SLC4 were downregulated in male compared to female embryos. In addition, 17.3% of the DEGs were located on the X chromosome and 75% of the DEGs located on the X chromosome were upregulated in female embryos. On the other hand, 38 genes including SLC30 A10, SLC10 A4, ATP6 AP1, and KDM5 C showed sexually dimorphic expression patterns in day 13 bovine embryos irrespective of the maternal environment. These genes accounted for only 19% and 13% of the genes that showed sexually dimorphic expression in embryos developed in cows and heifers, respectively and the expression difference of these genes in male and female embryos was then likely influenced by the sex of the embryo. Conclusion This study revealed that embryos developed in lactating cows showed sexually dimorphic expression of genes involved in various functions including steroid biosynthesis and gastrulation. In contrast, embryos developed in heifers displayed sexually dimorphic expression of genes related to placental development, female sex differentiation, and transmembrane transport. This suggests that the reproductive tract environments of cows and heifers differently affect the sex specific expression of genes in bovine embryos. A higher proportion of genes that showed sexually dimorphic expression in cow embryos were located on the X chromosome, and the majority of these genes were upregulated in female embryos. Overall, this study provides insight into genes that exhibit sexually dimorphic expression patterns in day 13 bovine embryos due to the maternal reproductive tract microenvironment or solely due to the sex of the embryo
Enhanced discovery of bacterial laccase‐like multicopper oxidase through computer simulation and metagenomic analysis of industrial wastewater
No full textLaccases belong to the superfamily of multicopper oxidases (MCO), a group of enzymes with the ability to reduce oxygen to water in a reaction without producing harmful byproducts. Laccase activity is influenced by many factors, such as structure; the number, location and binding status of copper ions; and the substrate‐binding status. A large number of sequences that have not been experimentally characterized yet have been annotated as laccases. However, the biological functions of the characterized MCOs are considered to vary, and the substrate spectrum overlaps with that of other MCOs. Here, we identified 34 putative bacterial laccase sequences from metagenome data for industrial wastewater. We used machine‐learning tools to screen enzymes with laccase activity by combining the T1 copper‐binding capacity, the overall copper‐binding capacity and the substrate‐binding capacity. We also used the software comparisons to remove sequences with large discrepancies between different software applications. Three‐dimensional structures of identified enzymes were predicted using alphafold , the positions of metal ions within the proteins were predicted by metal3d and autodock‐vina , and their docking with ABTS [i.e. 2,2′‐azinobis(3‑ethylbenzo‐6‑thiazolinesulfonic acid)] as a substrate was predicted by rosetta and autodock‐vina . Based on the docking results, we selected 10 high‐scoring proteins, two low‐scoring proteins and one composite protein for expression using the pET‐21d (+) vector. In line with our predictions, all selected high‐scoring proteins exhibited activity towards ABTS. Overall, we describe a method for discovering and designing novel bacterial laccase‐like multicopper oxidases, offering increased possibilities for the degradation of various harmful components derived from environmental pollution
