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Advanced fluorescence lifetime-enhanced multiplexed nanoscopy of cells
No full textIn this review paper, we summarize the significant advancements in the field of fluorescence lifetime imaging microscopy (FLIM), particularly wide-field FLIM with single-molecule sensitivity, achieved using the time-correlated single-photon counting-based position-sensitive LINCam system. Fluorescence lifetime adds valuable information beyond conventional intensity-based imaging, enabling diverse applications across research fields. Here, we focus on three primary bioimaging applications: (I) single-molecule FLIM in the far-red spectral region, (II) fast and multiplexed super-resolution imaging of cells, and (III) three-dimensional super-resolution imaging with high axial localization precision. Recent advances in position-sensitive detector technologies offer exciting opportunities for high-throughput super-resolution imaging with enhanced localization precision
Secondary bladder cancer following radiation therapy for prostate cancer: a systematic review and meta-analysis of incidence and outcomes
No full textExpression of “Hallmarks of Cancer” Genes in Cervical Carcinoma Is Differentially Affected by GPER1 Overexpression Depending on Histologic Entity
No full textOpen-Access-Publikationsfonds 202
Impact of drought stress on biochemical and molecular responses in lavender (Lavandula angustifolia Mill.): effects on essential oil composition and antibacterial activity
No full textDrought stress significantly influences the physiological, biochemical, and molecular processes in plants, directly impacting their growth and defense mechanisms. This study evaluates the response of Lavandula angustifolia (lavender) to different levels of water deficit, with field capacity (FC) treatments set at 20%, 40%, 60%, and 80%. We assessed various biochemical parameters, including protein content, chlorophyll a and b levels, flavonoid and phenolic content, and antioxidant activity, to determine how drought stress affects lavender’s primary and secondary metabolism. As water availability decreased, we observed a reduction in total protein and chlorophyll content, while the highest levels of flavonoids, phenolics, and antioxidant activity were recorded in control plants at 80% FC. Gene expression analysis of key terpene synthase genes revealed differential expression patterns, with linalool synthase and α-pinene synthase peaking at 40% FC, and 1,8-cineole synthase and β-phellandrene synthase reaching their highest activity under severe drought (20% FC). Despite this, a clear correlation between gene expression and metabolite accumulation in essential oils was not observed. Drought-induced changes in essential oil composition were associated with enhanced antibacterial activity, particularly against foodborne Gram-positive and Gram-negative bacteria, suggesting that water stress can modulate the therapeutic potential of lavender oil
Sphingolipidoses: expanding the spectrum of α-synucleinopathies
No full textAbstract Although α-synuclein pathology is typically associated with Lewy body diseases and multiple systems atrophy, increasing evidence indicates that it also occurs in a group of lysosomal storage disorders termed sphingolipidoses caused by the incomplete degradation, and subsequent accumulation, of a class of lipids termed sphingolipids. Notably, a number of genes that cause sphingolipidoses are also risk genes for Lewy body diseases, suggesting aetiological links between these distinct disorders. In the present review, we discuss the sphingolipidoses in which α-synuclein pathology has been reported: Gaucher disease, Krabbe disease, metachromatic leukodystrophy, Tay-Sachs disease and Anderson-Fabry disease, and describe the characteristic clinical and pathological features of these disorders, in addition to the evidence suggesting α-synuclein pathology occurs in these disorders. Finally, we evaluate the pathological mechanisms that underlie these rare disorders, with particular attention to how the enzymatic deficiency, substrate accumulation, or both, could contribute to the genesis of α-synuclein pathology and the implications of this for Lewy body diseases
Reference coverage analysis of OpenAlex compared to Web of Science and Scopus
No full textAbstract OpenAlex is a promising open source of scholarly metadata, and competitor to established proprietary sources, such as the Web of Science and Scopus. As OpenAlex provides its data freely and openly, it permits researchers to perform bibliometric studies that can be reproduced in the community without licensing barriers. However, as OpenAlex is a rapidly evolving source and the data contained within is expanding and also quickly changing, the question naturally arises as to the trustworthiness of its data. In this report, we will study the reference coverage and selected metadata within each database and compare them with each other to help address this open question in bibliometrics. In our large-scale study, we demonstrate that, when restricted to a cleaned dataset of 16.8 million recent publications shared by all three databases, OpenAlex has average source reference numbers and internal coverage rates comparable to both Web of Science and Scopus. We further analyse the metadata in OpenAlex, the Web of Science and Scopus by journal, finding a similarity in the distribution of source reference counts in the Web of Science and Scopus as compared to OpenAlex. We also demonstrate that the comparison of other core metadata covered by OpenAlex shows mixed results when broken down by journal, where OpenAlex captures more ORCID identifiers, fewer abstracts and a similar number of Open Access status indicators per article when compared to both the Web of Science and Scopus.Bundesministerium für Bildung und Forschung http://dx.doi.org/10.13039/501100002347HORIZON EUROPE Framework Programme http://dx.doi.org/10.13039/100018693GESIS – Leibniz-Institut für Sozialwissenschaften e.V
A case of fatal respiratory diphtheria imported from Poland to Germany: possible link to an undetected imported diphtheria cluster in Poland?
No full textEvaluating an early Interdisciplinary Multimodal Assessment for Patients at Risk of Developing Chronic Pain: Results of a Multicentre RCT in Germany
No full textExploring customer segmentation for food products with additional health benefits: A case study on iron-biofortified vegetables, functional foods, and dietary supplements
No full texthttp://dx.doi.org/10.13039/501100002347 Federal Ministry of Education and Research Bonn Officehttp://dx.doi.org/10.13039/501100000921 European Cooperation in Science and Technolog