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Polyethylene Glycol-Mediated Directional Conjugation of Biological Molecules for Enhanced Immunoassays at the Point-of-Care
Get PDFRapid and reliable point-of-care (POC) diagnostic tests can have a significant impact on global health. One of the most common approaches for developing POC systems is the use of target-specific biomolecules. However, the conjugation of biomolecules can result in decreased activity, which may compromise the analytical performance and accuracy of the developed systems. To overcome this challenge, we present a polymer-based cross-linking protocol for controlled and directed conjugation of biological molecules. Our protocol utilizes a bifunctional thiol-polyethylene glycol (PEG)-hydrazide polymer to enable site-directed conjugation of IgG antibodies to the surface of screen-printed metal electrodes. The metal surface of the electrodes is first modified with thiolated PEG molecules, leaving the hydrazide groups available to react with the aldehyde group in the Fc fragments of the oxidized IgG antibodies. Using anti-Klebsiella pneumoniae carbapenemase-2 (KPC-2) antibody as a model antibody used for antimicrobial resistance (AMR) testing, our results demonstrate a ~10-fold increase in antibody coupling compared with the standard N-hydroxysuccinimide (NHS)-based conjugation chemistry and effective capture (\u3e94%) of the target KPC-2 enzyme antigen on the surface of modified electrodes. This straightforward and easy-to-perform strategy of site-directed antibody conjugation can be engineered for coupling other protein- and non-protein-based biological molecules commonly used in POC testing and development, thus enhancing the potential for improved diagnostic accuracy and performance
Acceptance Governance
Get PDFThere is a form of power whereby the moral relationship governs those who are part of, or affected by, the relationship. Called “acceptance governance,” it develops “power-with in a decolonial manner, reframing agency as guided by accountability. Power-with leads to minimal moral relations between worlds out of which processes of acceptance build justice and right relations from the bottom up. There are two senses of acceptance, however, the second being accepting the conditions of acceptance. Power-with then becomes grounded in “power from,” a new form of power uncommon in the literature. In addition to cohering with a number of indigenous traditions as well as with ecological versions of civic republicanism, acceptance governance provides one way to internalize ecological reflexivity within norms of planetary governance and contributes to our analysis of institutional and personal capacities for participation, knowing, and healing from injustice common to significant strands of ESG research today
The History of the Flora Stone Mather Center for Women at Case Western Reserve University
Get PDFIn this article, we present the history of the Flora Stone Mather Center for Women at Case Western Reserve University, written around the time of its founding. We put this in conversation with the proposal which was submitted to university administration as justification for the creation of the Center for Women. In pairing the snippets of the proposal with details of the Mather Center’s present programming and resources, we are able to show how we are living up to the mission of our founders and the benefit to our campus community of having a women’s center on our campus
Does work-life boundary management improve work-life balance for remote workers: A critically appraised topic
Get PDFThis topic paper examines whether managing the boundaries between work life and personal life positively affects work-life balance in the context of remote work. The research that forms the basis of this topic paper is derived from five scholarly, peer-reviewed articles relevant to the stated purpose. The findings are mixed. In the long term, they indicate that successful management of the boundaries between work life and personal life affects work-life balance positively, while non-management of boundaries affects work-life balance negatively. In the short term, the findings demonstrate that managing boundaries in a way that allows for temporary and deliberate weakening of boundaries can also have a positive effect on work-life balance if the move accommodates a personal need or is perceived as a temporary imbalance. As remote working arrangements become more widespread and a larger emphasis is placed on the importance of work-life balance for employees, understanding the effect that remote work has on work-life balance is critical. As a result, the findings of this topic paper are relevant to current management challenges and to managers in all industries where remote working takes place
Bullying Victimization and Perpetration: Some Answers and More Questions
Get PDFThe U.S. government has defined bullying victimization as “any unwanted aggressive behavior(s) by another youth or group of youths who are not siblings or current dating partners that involves an observed or perceived power imbalance and is repeated multiple times or is highly likely to be repeated”.1 Conceptualizations of bullying and the use of the term vary by audience and context. For instance, for some, bullying behaviors might include acts of aggression or violence, whereas for others bullying might center on name-calling, exclusionary social practices or even spreading rumors and vicious lies.
Apheresis Collection of Mononuclear Cells for Chimeric-Antigen Receptor Therapies
Get PDFCollections of lymphocytes to be genetically modified to treat hematologic malignancies have seen a dramatic increase over the last few years as commercial products have been approved. Reports of new products in development that can possibly treat solid organ malignancies represent a massive change in the field. Apheresis is at the center of the collection of cells for the manufacture of these chimeric-antigen receptor therapy products. The expansion of these collections represents one of the areas of apheresis procedures growth. This review will summarize concepts important to this type of collection and variables that need to be optimized to obtain desired cell yields while increasing patients\u27 safety
A Novel Approach to Legacy Donations with Long-Term Benefits Supported by Numerical Illustrations
Get PDFPhilanthropic donors face challenges in matching the causes to which they donate, the time horizon—and thus impact—of their donations, and the charitable vehicles they choose for making contributions. Wealthier donors may elect to create their own foundations and customize their charitable support. Less wealthy donors have limited choices: they may contribute to a nonprofit\u27s current operations or to existing nonprofit endowments. We present a novel approach for making charitable donations, blending aspects of each of these strategies. Our approach has potential long-term financial benefits, allows donors to control their charitable donations in a convenient and easy-to-implement manner, can be established through an existing nonprofit organization, expands opportunities for more donors because it requires a smaller corpus contribution with lower management costs than creating a foundation, provides tax savings in the United States and other countries (e.g., the UK, Canada, and Australia) comparable to other planned giving vehicles, and may be implemented during one\u27s lifetime using donor advised funds or as part of a legacy plan through the donor\u27s estate documents, which is when the long-term benefits accrue
Team-Based Learning in the Internal Medicine Clerkship Didactics
Get PDFBackground: Active-learning approaches, such as team-based learning, are infrequently used in internal medicine clerkship didactics even though there is increasing evidence to suggest medical students prefer it over traditional lecture-based learning. In this study, five team-based learning sessions were incorporated into three blocks of a 12-week internal medicine clerkship. Methods: The goal of this quasi-experimental study was to compare learner engagement, satisfaction and preference between team-based learning and lecture-based learning in the internal medicine clerkship didactics. Outcomes were compared using the Classroom Engagement Survey, a satisfaction questionnaire and the Team-Based Learning Student Assessment Instrument (TBL-SAI). Findings: There was a statistically significant difference in the classroom engagement scores between team- and lecture-based learning (P \u3c 0.0001) with a median of 39.0 and 33.0 in the team-based learning and lecture-based learning groups. For learning preference, the median TBL-SAI score was substantially above neutral. Across all team-based learning sessions, 100% of students were satisfied or strongly satisfied with the learning style as a valuable experience and as a way to learn course material, and only one student was not satisfied or strongly satisfied with team-based learning to improve problem solving skills. Discussion: The classroom engagement and learning style preference findings were consistent with previously published data in other clerkship settings. Student satisfaction was more consistent with team-based learning than with the lecture-based learning, which may be because of the consistent format whereas lecture-based learning style was faculty dependent. Conclusion: Students preferred team-based learning and had improved engagement and satisfaction when compared to lecture-based learning. This study provides evidence in favour of team-based learning as a strategy to incorporate active learning in clerkship didactics
WSLDI: Women Staff Leadership Development Institute
Get PDFThis brief reviews the Case Western Reserve University Women Staff Leadership Development Institute, including participant evaluation data and national experiences of women staff in higher education.https://commons.case.edu/mathercenter-briefs/1001/thumbnail.jp
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