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Specific Changes in Arabidopsis thaliana Rosette Lipids during Freezing Can Be Associated with Freezing Tolerance
Get PDFWhile the roles of a few specific lipids in plant freezing tolerance are understood, the effect of many plant lipids remains to be determined. Acclimation of plants to non-freezing cold before exposure to freezing temperatures improves the outcome of plants, compared to plants exposed to freezing without acclimation. Arabidopsis thaliana plants were subjected to one of three treatments: (1) “control”, i.e., growth at 21 °C, (2) “non-acclimated”, i.e., 3 days at 21 °C, 2 h at −8 °C, and 24 h recovery at 21 °C, and (3) “acclimated”, i.e., 3 days at 4 °C, 2 h at −8 °C, and 24 h recovery at 21 °C. Plants were harvested at seven time points during the treatments, and lipid levels were measured by direct-infusion electrospray ionization tandem mass spectrometry. Ion leakage was measured at the same time points. To examine the function of lipid species in relation to freezing tolerance, the lipid levels in plants immediately following the freezing treatment were correlated with the outcome, i.e., ion leakage 24-h post-freezing. Based on the correlations, hypotheses about the functions of specific lipids were generated. Additionally, analysis of the lipid levels in plants with mutations in genes encoding patatin-like phospholipases, lipoxygenases, and 12-oxophytodienoic acid reductase 3 (opr3), under the same treatments as the wild-type plants, identified only the opr3-2 mutant as having major lipid compositional differences compared to wild-type plants
I want to poke my eyes out!: Why meetings are painful and tips on how to make them better
No full textAt one of the first meetings Tara Coleman attended as a new professional, Dove chocolate was distributed with the agenda to “sweeten the subject matter”. As the meeting progressed, she learned that the chocolate was a bribe to get people in the room, and a sign of tremendous boredom and irritation ahead. Soon she saw her colleagues turn the foil wrappers into little swords and stab themselves in the heart and eyes to show how their feelings on how the meeting was going. Spoiler alert, candy does not sweeten boring subject matter and foil swords are sharp enough to hurt the unguarded eyeball.
You know the ones, you’re not sure why you are in the room, one person dominates the conversation, nothing is accomplished, and you might leave with a completely different understanding of what happened than someone else. You might have even wanted to fake an injury to get out of it early. Meetings can be exhausting and leave you feeling like you wasted valuable time. And since the pandemic, for some meetings have gone from painful to torture. All at once folks needed to learn Zoom, struggle with shared wifi, and have fewer reasons to get up and move around.
While we can’t promise to make meetings a party, we can provide ways to make them less painful for everyone involved. If this proposal is selected, folks will receive tangible tips that will shape meeting expectations and roles, free or low cost tools that allow for voices to be heard that would otherwise be hidden or talked over, and sage advice about how to best use and respect everyone’s time
Fischer’s Fur Babies Veterinary Clinic
Get PDFI have wanted to be a veterinarian for as long as I can remember. I was accepted into veterinary school as a senior in high school through Kansas State University College of Veterinary Medicine’s Early Admittance Program. Throughout my college journey, I’ve focused on Animal Science, Spanish, and Risk Management. For my Honors Project, I decided to combine my future entrepreneurial plans with my desire to help animals by designing my own veterinary practice. Since I am passionate about both private practice and shelter medicine, this plan combines the unique aspects of both. The idea for Fischer’s Fur Babies Veterinary Clinic came from my experience with the Animal Rescue Foundation in Chicago, IL. Seeing the impact that these foster organizations have on the lives of thousands of homeless animals fueled my desire to help make a difference. That is why our clinic is so focused on helping shelters and foster organizations. We are paying homage to the volunteers who dedicate their lives to this neverending work. This report details the key attributes of a veterinary clinic that bridges the gap between private practice and shelter medicine. It will explore different aspects of vertical integration and various activities the veterinary clinic will participate in to help better the lives of animals
A Comparative Analysis of the American and Chinese Pharmaceutical Industries
Get PDFFollowing the growth of the Chinese pharmaceutical consumer market and innovative capability, comparisons between the American and the Chinese pharmaceutical industries have become commonplace due to two very divergent governmental, competitive, financial, and knowledge environments, as scholars and businesspeople alike attempt to predict shifts in power dynamics. Previous pharmaceutical industry research has focused on a single country, broad industry analysis (Ni, et al., 2017), or has conducted specific cross-border comparisons which fail to consider the respective macro-environments (Jiang & Luan, 2018; Zhao M. , 2021). This literary review examines four environments relevant to pharmaceutical innovation to achieve a broad and comparable understanding of the two industries, ultimately finding that the American industry is a well-established player focused on highly innovative activities, while the Chinese industry is a relative newcomer that is quickly developing innovative capabilities relevant to global competition dynamics
Supporting Information for “Robust Bayesian variable selection for gene-environment interactions”
Get PDFWater Contamination from Pesticides in Crop Runoff
Get PDFKirmser Undergraduate Research Award - Individual Freshman category, honorable mentionLaura MoleyWhen deciding on my research topic, I applied what I had learned in a few of my classes to the real world. Most places in Kansas get their drinking water from local streams and rivers, and it is a big issue if pesticides are flowing into the streams and rivers from crop runoff. In recent years, many places in Northeastern Kansas have had increased rainfall and there has been more runoff from the fields. Most fields are located in flood plains or near creeks and rivers. I thought it would be interesting to see how pesticides from crop runoff affect the local rivers and streams. This is an important topic that does not get discussed very often. I did some background reading to make sure this was the topic that I wanted to focus on, but it was my own family experience that refined my topic
Analyzing Isolates of Fusarium Head Blight in Wheat Across Kansas
No full textOne of the big threats to wheat farmers in Kansas is Fusarium Head Blight (FHB). This disease infects wheat heads, reducing the yield of the plant and releasing deoxynivalenol, a toxin that causes vomit sickness in humans and animals. This toxin is heavily regulated by the FDA making the sale of wheat seed infected with FHB nearly impossible and reducing the profit of that season. Preventing FHB in the field is difficult because wheat is only susceptible at heading, which makes the timing of fungicide application critical.. Research and communication between producers, breeders, and researchers attempt to find better ways of managing FHB. This project has collected samples of infected wheat heads from across Kansas from several different counties with the help of Kansas wheat breeders, Extension specialists, agents, ag industry professionals, and producers. Infected wheat heads are sterilized and the fungus is isolated. Fungal cultures underwent single-sporing to confirm a pure isolate. Then DNA is extracted and sequenced. This confirms that the fungus associated with the wheat head is Fusarium and what species. This process is done in hopes of different strains and a pattern in those differences across KS counties to develop more targeted methods of dealing with Fusarium Head Blight. This includes using these isolates in screening nurseries. This collaborative effort aims to help farmers respond more effectively to infection in the field and protect their crop and profit
Evaluating effect of cutting depths on regrowth of invasive cattails (Typha angustifolia)
Get PDF"Moorberg, C., Travis ,S., & Ahlers, A. (2022)Evaluating effect of cutting depths on regrowth of invasive cattails (Typha angustifolia). Preprint” supersedes "Moorberg, C. & Ahlers, A. (2020). An experimental evaluation of cattail (Typha spp.) cutting depths on subsequent regrowth. Preprint."Cattail (Typha spp.) expansions into wetland ecosystems can reduce open-water habitats and negatively affect both native flora and fauna diversity. Cattail removal is needed to maintain wetland habitat quality; however, removal is often non-permanent requiring repeated treatments to retard reestablishment. Cutting cattails with mechanical harvesters is a common management technique; however, it is unclear what cutting depths are optimal. We conducted a controlled, replicated experiment at Cheyenne Bottoms Wildlife Area near Great Bend, Kansas, USA during 2017-2018 to address this question. We hypothesized that cattails cut below water would have reduced gas exchange capabilities due to flooded aerenchyma resulting in greater mortality. We conducted genetic testing to determine the species of cattail and determined the cattails to be narrowleaf cattail (Typha angustifolia), which is considered an invasive species in Kansas. Within this stand of cattail, we established a randomized complete block design experiment with four blocks, and three clipping treatments in July 2017. The clipping treatments included a control (no cattails were clipped), an above-water treatment (cattails cut 15 cm above the surface of the water) and a below-water treatment (cattails cut 15 cm below the water surface water). We quantified emergent stem densities in each plot in September 2017 to assess the effectiveness of simulated management actions. We predicted lower emergent stem densities in below-water cutting treatment blocks compared to both the control and above-water cutting treatment blocks. Mean stem densities were greatest in the control (113.0±10.7 stems). Clipping the cattails resulted in significantly fewer stem counts in both the above-water cutting treatment (44.1±10.7, p=0.0032) and in the below-water cutting treatment blocks (11.1±12.5, p=0.0004). The BW treatment had fewer stems than the AW treatment, though this difference was not significant (p=0.0789) at an alpha of 0.05. Our experimental setup was inadvertently destroyed with herbicides in 2019 preventing further treatment comparisons. This is the first known genetic-level confirmation of narrowleaf cattail at Cheyenne Bottoms Wildlife Area, which is a Ramsar Wetland of International Importance. Our results suggest that management efforts focused on cutting cattails below water can reduce cattail growth
Photograph album, Hershberger no. 3
No full textThis album contains photographs and postcards from the 1930s of people and places in the Philippines where Frank C. Hershberger was stationed in the 1930s. Some photos are of people and bears in the United States. Locations in the Philippines represented by the photographs and postcards include Baguio, Bontoc (Mountain Province), Banaue, Manila, and Iloilo City. There are photographs of individuals and groups from the Cordilleran (labeled as Igorot in the photographs) indigenous groups of the Philippines including the Isneg (labeled as Apayao in the photographs) and Ifugao. There are photographs of some Cordilleran burial practices. There are also photographs of animals in the Philippines including carabao, dogs, and chickens. There are clippings of landscape drawings of the Mountain Province, as well as postcards from Singapore and Mexico City, Mexico. Some images on pages 15 and 17 have been removed from the digital copy as they include human remains, per Morse Department policy
“Our brokenness kind of connects us”: exploring social justice topics through read-alouds in a ninth-grade classroom
Get PDFDoctor of PhilosophyCurriculum and Instruction ProgramsLotta C. LarsonFor decades, K-12 teachers across the United States have read aloud to their students, whether it be to model fluent reading, to promote vocabulary acquisition, or out of pure enjoyment. As social justice becomes a more prevalent topic in classrooms across the country, interactive read-alouds are being used to introduce and discuss complex and delicate topics, like human rights and social justice. While students at all junctures of development and learning embrace and benefit from reading aloud, existing research primarily takes place in elementary school settings. Furthermore, literature used to explore social justice issues usually involves picture books rather than longer texts like chapter books. This study was designed to gain insight into how a classroom teacher facilitated a nonfiction chapter book read-aloud and how the students responded to the social justice themes represented in the chapter book. The study took place over the span of 18 days in a Midwest ninth-grade classroom. The theoretical underpinnings that framed the study were constructivism, transactional theory of reader response and critical literacy. Data was collected and analyzed using qualitative case study principles. Study results reveal four emerging themes across the research questions, including expressive reading; spontaneity; redemption; empathy; and awareness