imagine (Institute of molecular genetics and genetic engineering)
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Determination of hydrogen cyanide producing strains as potential biocontrol agents
Get PDFIntroduction: Hydrogen cyanide (HCN) is a volatile secondary metabolite synthesized by some bacteria, and this ability enablestheir activity against various pathogens. The aim of thisstudy wasto identify
HCN-producing bacteria and investigate their biocontrol potential.
Methods: Three HCN-producing strains were detected in a collection of bell pepper plant isolates using
a semi-quantitative assay with picric acid. The presence of hcnABC operon genes was confirmed by PCR.
The biological control potential of the HCN-producing strains wastested against three fungal (Fusarium
oxysporum, Rhizoctonia solani, Verticillium dahliae) and eight bacterial (genera Xanthomonas,
Pseudomonas and Clavibacter) pathogens of bell pepper plants in a split-section Petri dish experiment.
The potential nematocidal activity was demonstrated by using the Caenorhabditis elegans AU37 strain,
with temperature-sensitive sterility and enhanced sensitivity to pathogens.
Results: Detailed characterization of 300 isolates from our collection revealed that we have three different HCN-producing strains identified as Bacillussubtilis, Pseudomonas moraviensis, and P. putida, with
P. putida A32 being the most potent. Thisstrain is used for the deletion of the hcnB gene to confirm HCN
as a biocontrol agent.
Conclusion: The HCN-producing strains showed biocontrol potential against bacteria, fungi, and nematodes. It is concluded that the biological control activity isthe result of a volatile metabolite diffusing
through the air. Our future ex
SOX transcription factors – choosing between stemness and neuronal differentiation
Get PDFThe SOX genes show properties of both classical transcription factors and
architectural components of chromatin. As a main part of regulatory networks that
consist of transcription factors, epigenetic modifiers and microRNAs, they govern
diverse cellular processes during embryonic and adult neurogenesis, such as
maintaining the multipotency of neural stem cells, cell fate decision and terminal cell
differentiation.
The sequential expression of the SoxB, SoxC, SoxD, SoxE and/or SoxF group
members in differentiating and mature neurons, oligodendrocytes and astrocytes is
essential for their identity and survival. Several studies revealed that deregulated
expression of these proteins had significant impact on the neuronal plasticity. Results
of our research using both in vivo and in vitro model systems to study neurogenesis in
different brain pathologies, demonstrated decreased expression of SOXB transcription
factor in neural stem/progenitor cells in early stages of neurodegeneration or
following ischemia- related injury that has significant impact on regeneration
capacity. Recently, it has been demonstrated that neural stem cells from SVZ are cells
of origin of glioblastoma, the most common and devastated brain tumor. Our
investigation of SOXB showed deregulated expression of SOX3 protein also in
glioblastoma samples, compared to the expression in non-tumoral brain tissues while
exogenous overexpression of this gene promoted the malignant properties of
glioblastoma cells.
Despite numerous data implicating the key role of Sox/SOX genes in regulation
of neurogenesis, contribution of SOX genes in various brain pathologies is still not
clear. Future studies are needed to evaluate them as a potential targets in therapeutic
and regenerative strategies.BOOK OF ABSTRACTS: 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international
participation 31 May – 2 June 2023. Belgrade, Serbi
Nursing of Pike-Perch (Sander lucioperca) in Recirculating Aquaculture System (RAS) Provides Growth Advantage in Juvenile Growth Phase
Get PDFThis study aimed to estimate the efficacy of two pike-perch juvenile production technologies: exclusive Recirculating Aquaculture System (RAS) culture (the RAS group) and pond larviculture with a transfer to the RAS at the 42nd day post-hatch (DPH). Both direct weaning on dry feed (the Pond-D group) and 10-day gradual weaning using bloodworms (the Pond-B group) after transfer to the RAS were evaluated in pond-nursed fry. Their survival and morphometric indices were monitored after the RAS habituation period (first 10 days), after the 18-day post-habituation period and after an additional 30 days of on-grow. Our results indicate a negative allometric growth of the pond-nursed fish during the nursing period, which was slower (p < 0.0001) in comparison to the RAS-nursed fry (16.3 ± 0.4 vs. 17.8 ± 0.7%/day). After transfer, these fish grew faster than the RAS-nursed fry (7.7 ± 0.1, 4.9 ± 0.5 and 6.1 ± 0.6 during habituation, 8.5 ± 0.6, 9.3 ± 0.5 and 6.7 ± 0.1%/day during post-habituation period, in the Pond-B, Pond-D and RAS groups, respectively). However, four weeks afterwards, the RAS-nursed fry were again superior in terms of growth (4.0 ± 0.1, 3.6 ± 0.2 and 4.6 ± 0.2%/day, for the Pond-B, Pond-D and RAS groups, respectively), and this was accompanied by a significantly lower feed conversion ratio in this group. Although the survival of the RAS-nursed fry during the nursing period was lower in comparison to the pond-reared fry (11.3 vs. 67.3%), the RAS seems to provide a long-term growth advantage.Raw data: [https://imagine.imgge.bg.ac.rs/handle/123456789/2074
Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine
No full textIn the present study, synthesis of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine (1,6-naph), {[Ag(1,6-naph)(H2O)](BF4)}n (1) and [AuCl3(1,6-naph)] (2), was reported. The methods used for the structural characterization of a new compound 1 included IR, NMR (1H and 13C) and UV-Vis spectroscopy, cyclic voltammetry and single-crystal X-ray diffraction analysis. The crystallographic results showed that compound 1 represents silver(I) coordination polymer, in which 1,6-naph ligand acts as a bidentate bridging ligand connecting two Ag(I) ions via its N1 and N6 nitrogen atoms, while the third coordination site of the metal ion is occupied by the water oxygen atom, resulted in a T-shape geometry. Compounds 1 and 2 were evaluated in vitro for antimicrobial activity against five bacterial and two Candida species, while their cytotoxicity was tested on the normal human lung fibroblast cell line (MRC-5). Compound 1 has manifested a remarkable antifungal activity on both tested Candida strains (C. albicans and C. parapsilosis) with minimal inhibitory concentrations (MICs) of 1.43 and 11.38 µM (0.49 and 3.9 µg/mL), respectively, while no significant antimicrobial activity was observed for 2. Moreover, silver(I) coordination polymer 1 inhibits the hyphae formation of C. albicans at subinhibitory concentration. The binding affinity of both compounds 1 and 2 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy, indicating their ability to interact with these biomolecules, with compound 2 being more reactive.Related to published version: [https://imagine.imgge.bg.ac.rs/handle/123456789/2067
B-cell receptor stereotypy in chronic lymphocytic leukemia
Get PDFOpsežne imunogenetičke studije sprovedene tokom proteklih nekoliko decenija u oblasti hronične limfocitne
leukemije (HLL) su identifikovale grupe pacijenata koji eksprimiraju visoko homologne, skoro identične
imunoglobulinske molekule koji ulaze u sastav B-ćelijskog receptora (BĆR IG). Ovaj fenomen, nazvan
„BĆR stereotipija“, obuhvata oko 30% HLL i predstavlja najvažniju potvrdu uloge antigenske stimulacije u
patogenezi i evoluciji bolesti. Na osnovu sekvence CDR3 regiona teških lanaca IG definisani su stereotipni
subsetovi koji ispoljavaju konzistentne subset-specifične biološke i kliničke karakteristike. Neki od najzastupljenijih
subsetova imaju i jasan prognostički značaj, tako da se čak mogu smatrati različitim varijantama
bolesti. Subklasifikacija HLL bazirana na BĆR stereotipiji je omogućila kompartmentalizovan pristup u istraživanju
ove bolesti, i otvorila mogućnost za precizniju stratifikaciju pacijenata, kao i uspešniju prognostiku
i lečenje, uprkos izrazitoj kliničkoj heterogenosti HLL.During the past several decades, extensive immunogenetic research of chronic lymphocytic leukemia
(CLL) led to the identification of groups of patients expressing highly homologous, almost identical B-cell
receptor immunoglobulin molecules (BCR IG). This phenomenon, termed „BCR stereotypy“, accounts for
around 30% of all CLL and represents the strongest evidence for the role of antigenic stimulation in CLL
pathogenesis and evolution. Based on the sequence of CDR3 region within IG heavy chains multiple stereotyped
subsets have been defined, which display consistent subset-specific biological and clinical characteristics.
Some of the most frequent subsets also exert strong prognostic significance and can be
considered to be distinct disease variants. Stereotypy-based CLL subclassification enabled compartmentalized
approach in the research of this disease, and offered the possibility of refining stratification of patients
that can improve prognostication and clinical decision-making despite extreme heterogeneity of
CLL
An Overview of S-Genotype Diversity in Sweet Cherry Landraces Grown in the Central Region of the Republic of Serbia
Get PDFIdentification of the S-genotypes in landraces is a crucial step in the molecular characteri- zation of Serbian autochthonous sweet cherry germplasm. It is also of enormous significance for bre- eders and growers, as this fruit species exhibits a gametophytic self-incompatibility, controlled by the multi-allelic two genes of the S-locus. The aim of this study was to summarize known information and reveal new data on the S-alleles in 23 sweet cherry landraces originating in the Republic of Ser- bia. The use of polymerase chain reaction (PCR) with consensus primers for the second intron of S- RNase, primers specific for S-RNase and certain SFB alleles, along with DNA fragment analysis using fluorescently labelled forward primers to amplify both the S-RNase first intron and the SFB in- tron, revealed 10 alleles (S1 to S6, S9, S12, S13 and S22) that generated the following 13 S-genotypes: S1S2 (one landrace), S1S4 (one landrace), S1S5 (one landrace), S2S3 (four landraces), S3S4 (two lan- draces), S3S5 (two landraces), S3S6 (three landraces), S3S9 (two landraces), S3S12 (two landraces), S4S5 (one landrace), S4S13 (one landrace), S5S22 (one landrace) and S6S9 (two landraces). The most frequent S-allele and S-genotype in this sweet cherry material were S3 and S2S3, with occurrence fre- quencies of 32.6% and 17.4%, respectively. Based on the obtained results, the sweet cherry landraces were assigned to 12 incompatibility groups and one group of universal donors (‘0’). These results pro- vide important information about their cross-compatibility and the diversity of the S-locus in Serbian sweet cherry germplasm
Dynamic and Assembly Characteristics of Deep-Cavity Basket Acting as a Host for Inclusion Complexation of Mitoxantrone in Biotic and Abiotic Systems
Get PDFWe describe the preparation, dynamic, assembly characteristics of vase-shaped basket 13− along with its ability to form an inclusion complex with anticancer drug mitoxantrone in abiotic and biotic systems. This novel cavitand has a deep nonpolar pocket consisting of three naphthalimide sides fused to a bicyclic platform at the bottom while carrying polar glycines at the top. The results of 1H Nuclear Magnetic Resonance (NMR), 1H NMR Chemical Exchange Saturation Transfer (CEST), Calorimetry, Hybrid Replica Exchange Molecular Dynamics (REMD), and Microcrystal Electron Diffraction (MicroED) measurements are in line with 1 forming dimer [12]6−, to be in equilibrium with monomers 1(R)3− (relaxed) and 1(S)3− (squeezed). Through simultaneous line-shape analysis of 1H NMR data, kinetic and thermodynamic parameters characterizing these equilibria were quantified. Basket 1(R)3− includes anticancer drug mitoxantrone (MTO2+) in its pocket to give stable binary complex [MTO⊂1]− (Kd=2.1 μM) that can be precipitated in vitro with UV light or pH as stimuli. Both in vitro and in vivo studies showed that the basket is nontoxic, while at a higher proportion with respect to MTO it reduced its cytotoxicity in vitro. With well-characterized internal dynamics and dimerization, the ability to include mitoxantrone, and biocompatibility, the stage is set to develop sequestering agents from deep-cavity baskets
Toxicity testing of D, L-sulforaphane in a zebrafish model
Get PDFSulforaphane (SFN) belongs to the group of isothiocyanates and is present in many cruciferous plants,
especially broccoli. The positive properties of this bioactive molecule in the form of extracts have been
shown in numerous studies and include antibacterial, cardioprotective, and neuroprotective effects,
antioxidant and immunomodulatory effects, as well as positive effects in various cancers.
However, the potential for harmful effects of SFN has not been sufficiently investigated, particularly for
chemically obtained D,L-sulforaphane. This study aimed to investigate the toxicity of D,L-sulforaphane
on zebrafish (Danio rerio) model. Wild (AB) strain embryos and zebrafish transgenic lines with fluorescently
labeled liver cells (Tg(fabp:EGFP)) and endothelial cells of blood vessels (Tg(fli1:EGFP)) were used,
treated with different concentrations of SFN (1 to 20 μg/mL).
The survival of embryos, developmental toxicity, hepatotoxicity and cardiotoxicity were monitored for
five days. A concentration of 20 μg/mL of D,L-sulforaphane caused the death of all embryos, while the
median lethal concentration (LC50) was found to be 14.2 μg/mL. D,L-sulforaphane exhibited toxic
effects at concentrations higher than 3 μg/mL, primarily on the development of the swim bladder (4
μg/mL), and growth and development of embryos (4.58 μg/mL), while harmful effects on the liver (liver
size and yolk resorption) were observed at a concentration of 10 μg/mL. Effects on the cardiovascular
system were not observed at concentrations from 1 to 10 μg/mL. The investigation of D,L-sulforaphane
on zebrafish embryos showed that harmful effects occur at very low concentrations, indicating the
need for further investigation of toxicological potential of this molecule
Interakcije eNOS i ACE gena i duvanskog dima u hroničnoj opstruktivnoj bolesti pluća
Get PDFBackground: Chronic obstructive pulmonary disease(COPD) is a complex disorder with unexplained heritability.Interactions of genetic and environmental factors arethought to be crucial in COPD. So, we aim to examineinteractions of the endothelial nitric oxide synthase (eNOS)and angiotensin converting enzyme (ACE) genes and ciga-rette smoking in COPD. Methods:The eNOS G894T and ACE ID variants wereanalyzed in 122 COPD patients and 200 controls fromSerbia. The effect of the variants on COPD was assessed bylogistic regression. Interactions between eNOS, ACE andcigarette smoking in COPD were evaluated using a case-control model. Interaction between the genes was analyzedin silico. Results:No effect of the eNOS G894T and ACE ID variantson COPD was found in our study. Gene-gene interactionbetween the eNOS TT and ACE D was identified(p=0.033) in COPD. The interaction is realized within the complex network of biochemical pathways. Gene-environ-ment interactions between the eNOS T and cigarettesmoking (p=0.013), and the ACE II and cigarette smoking(p=0.009) were detected in COPD in our study. Conclusions:This is the first research to reveal interactionsof the eNOS and ACE genes and cigarette smoking inCOPD progressing our understanding of COPD heritabilityand contributing to the development of appropriate treat-ments.Uvod: Hronična opstruktivna bolest pluća (HOBP) je složeno oboljenje sa nerazjašnjenom genetičkom osnovom.Smatra se da su interakcije genetskih i spoljašnjih faktora ključne u HOBP. Stoga je naš cilj bio da ispitamo interakcijegena za endotelijalnu azot-monoksid sintazu (eNOS) i angiotenzin konvertujući enzim (ACE) i duvanskog dima uHOBP. Metode :eNOS G894T i ACE ID varijante su analiziranekod 122 HOBP pacijenta i 200 kontrola iz Srbije. Uticajvarijanti na HOBP je ispitan logističkom regresijom. Interakcije izme|u eNOS, ACE i duvanskog dima u HOBP suispitane korišćenjem modela slučaj-kontrola. Interakciaja između gena je analizirana in silico. Rezultati: Prema ovoj studiji eNOS G894T i ACE ID varijante nemaju uticaj na HOBP. Gen-gen interakcija između eNOS TT i ACE D je identifikovana (p=0,033) u HOBP.Ova interakcija se ostvaruje u okviru složene mreže bio-hemijskih puteva. Gen-sredina interakcije izme|u eNOS Ti duvanskog dima (p=0,013), i ACE II i duvanskog dima(p=0,009) su detektovane u HOBP u ovoj studiji.Zaključak: Ovo je prvo istraživanje u kome su otkriveneinterakcije između eNOS i ACE gena i duvanskog dima uHOBP što doprinosi našem razumevanju genetičke osnove HOBP i razvoju adekvatnog tretmana
Non-woven sorbent based on recycled jute fibers for efficient oil spill clean-up: From production to biodegradation
Get PDFLoose assemblies of cellulose fibers could be employed in the clean-up of oil spills, but the difficulty to separate them from the spill after use makes them impractical. In an effort to tackle this issue, a non-woven sorbent based on recycled jute fibers from the carpet industry was developed. To enhance the porosity and hydrophobicity/oleophilicity of the sorbent, carbonization in an inert atmosphere was carried out. A comparison in oil sorption performance between non-carbonized and carbonized sorbents was made by evaluating the oil capacity in a water medium, buoyancy, oil retention and reusability. Carbonization of sorbent resulted in more than doubled oil sorption capacity independent of oil viscosity. Oil viscosity did not affect the oil sorption capacity of non-carbonized sorbent. Carbonized sorbent showed superior buoyancy in water even after 24 hours, oil retention of approximately 60–80% after 3 hours and only 12–20% decline of oil sorption capacity after five repeated sorption/desorption trials. However, the ability of oiled non-carbonized sorbent to efficiently biodegrade in model compost (up to 45% weight loss after 10 weeks) makes it a sustainable candidate for oil spill clean-up