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EcoPlastiC: Turning Mixed PET Waste into High- Performance Biopolymers
No full textThe EcoPlastiC project (“Eco conversion of lower-grade PET and mixed recalcitrant PET
plastic waste into high-performance biopolymers”) is an EIC Pathfinder programme under
Horizon Europe. It aims to provide scalable and modular solutions for the valorisation of
unrecyclable mixed and PET-based post-consumer waste by advancing depolymerisation,
bioconversion, and recovery technologies that enable sustainable eco-products within a
perpetually bio-cyclable loop.
EcoPlastiC has achieved significant milestones across its technology pipeline:
1. A fully developed Depolymerisation process:
Development of mechano-green, chemical, and biocatalytic technologies to break down
PET consumer waste into fermentable feedstocks, such as terephthalic acid (TPA)
monomer. Upcycled PET and textile waste have also been converted into bacterial
nanocellulose.
2. Biopolymer Production:
Microbiome processing enables the generation of novel biopolymers, such as
polyhydroxyalkanoates (PHAs) and microbial proteins from monomer feedstocks.
Green recovery processing achieved >97% recovery rates for PHA, PHB, and PHBV.
3. Advanced Processing and Prototypes:
Recovered PHAs and microbial proteins are being developed into application-ready
products, including high-performance circular films with strong oxygen barrier
properties for food packaging and bio-adhesives as a high-functionality, low-volume
coating or adhesive with fully circular lifecycles.
By combining innovative processing with comprehensive sustainability assessments (i.e. life
cycle analysis (LCA)), EcoPlastiC demonstrates the potential for energy, carbon-, and cost-
efficient conversion of PET waste plastics into valuable bioproducts as alternatives to
conventional plastics, promoting seamless industrial and consumer adoption. This poster will
present the project’s integrated workflow, technological achievements and market adoption.
EcoPlastiC ultimately aims to deliver environmentally and economically sustainable
alternatives to fossil-based plastics.Global Polymer
Sustainability & Innovation Summit, 29-31 October 2025 , TUS, Athlone, Irelan
Corn stover-based biocomposites for circular and sustainable design
No full textThe data and files contained in this dataset are referring to the study investigated the development and end-of-life destiny of novel biocomposites labeled as SferiCorn, made from ground corn stover (CS) and three polymer matrices: starch (Sferi R), alginate (Sferi S), and poly(hydroxybutyrate-co-hydroxyhexanoate), PHBH, (Sferi BioTP). Produced without additives, the composites were analyzed for thermal behavior, swelling, structure, morphology, and degradation under composting, hydrolytic, and enzymatic conditions. It was concluded that polymer matrix type significantly affected SferiCorn biocomposites performances: starch-based composites degraded quickly while alginate-based indicated lower weight loss, and finally PHBH-based composite offered remarkable stability. Mechanical testing suggested that Sferi BioTP was suitable for prototyping, demonstrated by a designing a sustainable coata hanger as an appropriate substitution of currently used plastic and metal ones. In a circular bio-economy context, hydrolysates from degraded SferiCorn were upcycled into bacterial nanocellulose (BNC), resulting in a biopolymer production in the yield higher than the BNC cultivated in the glucose-based media. This work highlights the potential of using CS as a filler, but also that polymer matrix selection enabled tailored biodegradability for sustainable, application-specific designs.readme.txt (15.71Kb)***Dataset contents*** DSC PHBH.csv (90.13 Kb), DSC Sferi BioTP.csv (91.33 Kb), DSC Sferi R.csv (93.05 Kb), DSC Sferi S.csv (89.64 Kb), DSC SferiCorn biocomposites.png (70.65 Kb), DTGA PHBH.csv (85.55 Kb), DTGA Sferi BioTP.csv (83.83 Kb), DTGA Sferi R.csv (84.54 Kb), DTGA Sferi S.csv (84.45 Kb), DTGA SferiCorn biocomposites.png (81.64 Kb), FTIR BNC 1c.csv (136.04 Kb), FTIR BNC 2a.csv (136.73 Kb), FTIR BNC 2b.csv (137.00 Kb), FTIR BNC 2c.csv (136.73 Kb), FTIR BNC.PNG (57.17 Kb), FTIR Sferi R blanc before exp.csv (133.66 Kb), FTIR Sferi R blanc.csv (133.66 Kb), FTIR Sferi R buffer 2w.csv (133.72 Kb), FTIR Sferi R buffer 4w.csv (136.52 Kb), FTIR Sferi R cellulose 2w.csv (133.69 Kb), FTIR Sferi R cellulose 4w.csv (135.95 Kb), FTIR Sferi R cellulose buffer.PNG (43.07 Kb), FTIR Sferi R compost 10days.csv (136.03 Kb), FTIR Sferi R compost 17days.csv (134.80 Kb), FTIR Sferi R compost blanc.csv (133.66 Kb), FTIR SferiBioTP blanc before exp.csv (133.66 Kb), FTIR SferiBioTP blanc.csv (134.99 Kb), FTIR SferiBioTP buffer 2w.csv (134.32 Kb), FTIR SferiBioTP buffer 4w.csv (134.32 Kb), FTIR SferiBioTP cellulose 2w.csv (133.28 Kb), FTIR SferiBioTP cellulose 4w.csv (137.00 Kb), FTIR SferiBioTP cellulase buffer.PNG (60.98 Kb), FTIR SferiBioTP compost 10days.csv (134.81 Kb), FTIR SferiBioTP compost 17days.csv (134.91 Kb), FTIR SferiBioTP compost blanc.csv (134.96 Kb), FTIR SferiBioTP compost.PNG (48.51 Kb), FTIR SferiR SferiS SferiBioTP blanc before exp.PNG (40.55 Kb), FTIR SferiS blanc before exp.PNG (136.20 Kb), FTIR SferiS blanc.csv (136.00 Kb), FTIR SferiS buffer 2w.csv (134.57 Kb), FTIR SferiS buffer 4w.csv (134.19 Kb), FTIR SferiS cellulase 2w.csv (134.57 Kb), FTIR SferiS cellulase 4w.csv (134.00 Kb), FTIR SferiS cellulase buffer.PNG (45.12 Kb), FTIR SferiS compost 10days.csv (134.43 Kb), FTIR SferiS compost 17days.csv (134.43 Kb), FTIR SferiS compost blanc.csv (136.00 Kb), FTIR SferiS R compost.PNG (47.59 Kb), Stress-strain Sferi BioTP.csv (29.24 Kb), Stress-strain Sferi S.csv (55.20 Kb), Stress-strain.png (55.40 Kb), TGA PHBH.png (86.28 Kb), TGA Sferi BioTP.csv (87.11 Kb), TGA Sferi R.csv (89.36 Kb), TGA Sferi S.csv (89.36 Kb), TGA SferiCorn biocomposites.png (66.76 Kb)File readme.txt (15.71Kb) is under licence public domain CC
Primena najnovijih dostignuća u izotermalnim tehnologijama u biomedicinskoj molekularnoj dijagnostici
No full textThe rapid evolution of molecular diagnostics is driving a shift toward decentralized, fast, and highly
sensitive methods for nucleic acid detection. Isothermal amplification techniques, which eliminate the
need for thermal cycling, have emerged as strong, clinically viable alternatives to traditional PCR. These
approaches enable sensitive detection of pathogens causing infectious diseases as well as disease
biomarkers and genetic alterations, including single-nucleotide variants and short non-coding RNAs such
as microRNAs, providing insights into disease at the molecular level. This places isothermal molecular
technologies at the forefront of early detection in biomedicine. Techniques such as Loop-mediated
Isothermal Amplification (LAMP), Rolling Circle Amplification (RCA), and Exponential Amplification
Reaction (EXPAR) offer key advantages in target versatility, analytical sensitivity, and integration into
portable, point-of-care diagnostic platforms. Moreover, their compatibility with CRISPR/Cas systems further
enhances specificity and allows for high-throughput multiplexing. We provide a comprehensive review
of isothermal amplification methodologies, focusing on their use in diagnostic purposes in biomedicine,
assessing their integration with emerging molecular tools, and evaluating their potential applications in
personalized therapies, point-of-care diagnostics, and longitudinal disease monitoring.Brzi razvoj molekularne dijagnostike teži ka decentralizovanim, brzim i visoko osetljivim metodama za detekciju
nukleinskih kiselina. Tehnike izotermalne amplifikacije, koje eliminišu potrebu za termičkim ciklusima,
pojavile su se kao moćne i klinički primenljive alternative tradicionalnoj metodi PCR. Ovi pristupi
omogućavaju precizno otkrivanje patogena koji izazivaju zarazne bolesti, kao i biomarkera bolesti i
genetičkih promena, uključujući varijante jednog nukleotida i kratke nekodirajuće RNK kao što su
mikroRNK, pružajući uvid u bolesti na molekularnom nivou. Ovo stavlja izotermalne molekularne
tehnologije u prvi plan za rano otkrivanje bolesti u biomedicini. Tehnike kao što su Loop-mediated
Isothermal Amplification (LAMP), Rolling Circle Amplification (RCA), i Exponential Amplification Reaction
(EXPAR), nude značajne prednosti u različitosti ciljeva koji se mogu detektovati, analitičkoj osetljivosti i
njihovoj integraciji u prenosive „point-of-care“ dijagnostičke platforme. Štaviše, njihova kompatibilnost sa
CRISPR/Cas sistemima dodatno poboljšava specifičnost i omogućava sveobuhvatnu dijagnostiku brojnih
genetičkih markera. Ovaj rad predstavlja sveobuhvatan pregled metodologija izotermalne amplifikacije,
fokusirajući se na njihovu upotrebu u dijagnostičke svrhe u biomedicini, procenjujući njihovu integraciju
sa novim molekularnim alatima i procenjujući njihove potencijalne primene u personalizovanim
terapijama, „point-of-care“ dijagnostici i longitudinalnom praćenju bolesti
Diverse career paths within thrombosis and hemostasis: perspectives from the ISTH early career committee
No full textThe combined fields of thrombosis and haemostasis (T&H) offer extensive opportunities for interdisciplinary professional establishment and collaborations, as highlighted in an editorial by the International Society on Thrombosis and Haemostasis’ (ISTH) early career (EC) committee in 2024 [1]. The dynamic nature of clinical and basic research in the field, driven by evolving technologies and researchers’ ever-present goal to improve our understanding and management of thrombotic/haemostatic conditions, gives rise to diverse professions. For EC professionals (ECPs) who often train in an academic environment, this variety in career paths and abundance of transferable skills obtained during their training may not be immediately apparent. In addition, availability of mentoring and career coaching resources for ECPs varies within different institutions
Beta thalassemia syndromes: New insights
No full textBeta thalassemia (β-thalassemia) syndromes are a heterogeneous group of inherited hemoglobinopathies caused by molecular defects in the beta-globin gene that lead to the impaired synthesis of beta-globin chains of the hemoglobin. The hallmarks of the disease include ineffective erythropoiesis, chronic hemolytic anemia, and iron overload. Clinical presentation ranges from asymptomatic carriers to severe anemia requiring lifelong blood transfusions with subsequent devastating complications. The management of patients with severe β-thalassemia represents a global health problem, particularly in low-income countries. Until recently, management strategies were limited to regular transfusions and iron chelation therapy, with allogeneic hematopoietic stem cell transplantation available only for a subset of patients. Better understanding of the underlying pathophysiological mechanisms of β-thalassemia syndromes and associated clinical phenotypes has paved the way for novel therapeutic options, including pharmacologic enhancers of effective erythropoiesis and gene therapy.
Dordevic A, Mrakovcic-Sutic I, Pavlovic S, Ugrin M, Roganovic J. Beta thalassemia syndromes: New insights. World J Clin Cases 2025; 13(10): 100223 [PMID: 40191679 DOI: 10.12998/wjcc.v13.i10.100223
Značaj povišenog bazalnog 17-hidroksiprogesterona u dijagnostici dece sa kongenitalnom adrenalnom hiperplazijom
No full textIntroduction: Congenital adrenal hyperplasia (CAH) is characterized by a deficiency of 21α-hydroxylase causing a deficiency of cortisol and aldosterone and overproduction of 17-hydroxyprogesterone. The classic and non-classical forms of the disease present with signs of precocious puberty (PP) and accelerated body and bone growth. Elevated basal and stimulated 17-hydroxyprogesterone and genetic testing are crucial for confirming a definitive diagnosis.Aim: To determine the significance of elevated basal 17-hydroxyprogesterone in children with signs of precocious puberty in the final diagnosis of Congenital Adrenal Hyperplasia.Material and methods: A prospective study was conducted at the University Children's Clinic and the Institute of Molecular Genetics and Genetic Engineering in Belgrade from 2019 to 2024. The study involved 64 subjects of both sexes, aged up to 18 years, with precocious puberty, accelerated bone and body growth, elevated basal 17-hydroxyprogesterone, who were divided into two groups based on the presence/absence of pathogenic variants in the CYP21A2 gene. The anthropometric measures, skeletal maturation and hormone levels were compared between those two groups.Results: The research includes 64 subjects, divided into two groups, with confirmed CAH (30 subjects) and with PP as the control group (34 subjects). A statistically significant difference was shown in basal (p=0.000000807) and stimulated 17-hydroxyprogesterone (p= 0.0125), cortisol (p= 0.0148) and androstenedione (p= 0.014) in homozygous carriers of pathogenic variants in the CYP21A2 gene.Conclusion: Clinical and laboratory parameters such as precocious puberty and 17-hydroxyprogesterone may be significant hints to consider a carrier mutation for congenital adrenal hyperplasia.Uvod: Kongenitalna adrenalna hiperplazija (KAH) je obo -
ljenje u kom nedostatak 21a-hidroksilaze uzrokuje de ficit
kortizola i aldosterona, sa posledi~nim nagomilavanjem 17-
hidroksiprogesterona. Klasi~nu i neklasi~nu formu bo lesti
predstavljaju ambivalentne genitalije sa znacima pre -
vremenog puberteta (PP) puberteta (PP), ubrza nog teles nog
i ko{tanog sazrevanja. Klini~ki znaci zahtevaju laboratorijsku
(povi{en bazalni i stimulisani 17-hidroksiprogesteron) i ge ne -
ti~ku potvrdu kona~ne dijag noze. Cilj studije je utvrditi zna~aj
povi {enog bazalnog 17-hidroksi progesterona kod dece sa
znacima prevremenog puberteta u potvrdi dijagnoze
kongenitalne adrenalne hiperplazije.
Metode: Prospektivna studija sprovedena je u Univer zi tet -
skoj de~ijoj klinici i Institutu za molekularnu genetiku i
geneti~ko in`enjerstvo u Beogradu od 2019. do 2024.
godine. U studiji je u~estvovalo 64 ispitanika oba pola,
uzrasta do 18 godina, sa prevremenim pubertetom, ubrza -
nim ko{tanim i telesnim rastom, povi{enim bazalnim 17-
hidroksiprogesteronom, koji su podeljeni u dve grupe na
osnovu prisustva/odsustva patogenih varijanti u genu
CYP21A2. Obavljen je fizikalni pregled, antropometrijska
merenja, procena skeletnog sazrevanja, uzeta krv za bio -
hemijske i geneti~ku analizu, na osnovu kojih su ove dve
grupe ispitanika upore|ivane.Rezultati: Istra`ivanjem je obuhva}eno 64 ispitanika, po -
deljenih na dve grupe, sa potvr|enom dijagnozom KAH
(30 ispitanika) i sa PP kao kontrolnom grupom (34 ispi -
tanika). Pokazana je statisti~ki zna~ajna razlika u bazalnom
(p<0,001) i stimulisanom 17-hidroksiprogesteronu
(p=0,013), kortizolu (p=0,015) i androstenedionu (p=
0,014) kod homozigotnih nosioca patogenih varijanti u
genu CYP21A2.
Zaklju~ak: Klini~ki i laboratorijski pokazatelji poput pre -
vremenog puberteta i 17-hidroksiprogesterona mogu biti
zna~ajni indikatori nosioca varijanti odgovornih za razvoj
kongenitalne adrenalne hiperplazije
Lung Microbiota: From Healthy Lungs to Development of Chronic Obstructive Pulmonary Disease
No full textLung health is dependent on a complex picture of the lung microbiota composed of bacteriobiota, mycobiota, and virome. The studies have demonstrated that the lung microbiota has a crucial role in host protection by regulating innate and adaptive lung immunity. Chronic obstructive pulmonary disease (COPD) is an inflammatory lung disease featuring changed microbiota composition and diversity, known as a dysbiosis. The lung dysbiosis increases with the progress of COPD and during exacerbation. Two models of dysbiosis have been proposed: dysbiosis and inflammation cycles and the disturbance of bacterial interactome. Still, it is unknown if the driving factor of the pathogenesis of COPD belongs to the host or microbiota. Recently, host–microbiota and microbe–microbe interactions have been highlighted in COPD, but the mechanisms behind these interactions need further exploration. The function of the gut–lung axis is crucial for the maintenance of lung health and is affected in COPD. The application of probiotics has resulted in host–beneficial effects, and it is likely that future progress in this field will aid in the therapy of COPD. In this review, the composition of the lung microbiota, molecular mechanisms, and clinical aspects relating to host and microbiota in health and COPD are comprehensively provided
Spontaneous Cyclization of 2-Methylthiosemicarbazones Yields New 1,2,4-Triazolidine-3-thiones, Useful as Building Blocks for Drug Design
No full textFrom the point of view of medicinal chemistry, thiosemicarbazones are interesting molecules as they exhibit various biological activities, such as antitumor, antifungal, antibacterial, antiviral, and antileukemic properties, and their synthesis is deeply investigated. However, the synthesis using 2-methylthiosemicarbazide as a starting compound to prepare new 1,2,4-triazolidine-3-thione compounds via 2-methylthiosemicarbazone cyclization has not been reported yet. Therefore, fifteen new 1,2,4-triazolidine-3-thione compounds were synthesized during a cyclization reaction using a simple green procedure with minimal use of hazardous solvents. The synthesized compounds were characterized with the techniques of NMR, IR, MS, and CHN elemental analysis. In addition, the structure of 11 of these compounds was confirmed by X-ray structure determination. Selected compounds were subjected to cytotoxicity assay and microbiological tests for antimicrobial susceptibility and quorum sensing inhibition and tested for their inhibitory effect on the metalloenzyme NDM-1, which causes antimicrobial resistance
Supplementary information for the article: Zeljic K, Pavlovic D, Stojkovic G, et al. Analysis of TNS3-203 and LRRFIP1-211 Transcripts as Oral Cancer Biomarkers. Journal of Oral Pathology & Medicine. n/a(n/a). doi:10.1111/jop.13606
No full textTable 1 Hazard ratio Cox-regression analysis of demographic, clinicopathological
characteristics, TNS3-203 and LRRFIP1-211 expression in oral cancer tissue with patients
survival; Fig 1 Prediction of CpG islands and binding sites of transcriptional factors in a sequences of
TNS3 (a) and LRRFIP1 (b) gene promoters; Fig 2 Schematic representation of TNS3-203 (a) and LRFFIP1-211 (b) transcripts aligned
with the reference transcript of TNS3 and LRRFIP1 genes. According to Ensembl, TNS3-201 and
LRRFIP1-203 are reference transcripts of TNS3 and LRRFIP1 genes, respectively. The unique sequence of
TNS3-203 and LRRFIP1-211 is shown in darker colour.Related to published version:[https://imagine.imgge.bg.ac.rs/handle/123456789/2767
Izmenjeni nivoi sfingolipidnih metabolita u serumu pacijenata sa lokalno uznapredovalim karcinomom rektuma: pilot studija
No full textBackground: Altered sphingolipid levels might contribute
to rectal cancer development, progression and therapy
response by regulating various biological processes, including apoptosis. This study aimed to analyse the serum
sphingolipid profile in rectal cancer patients and investigate
its association with the apoptotic status of tumour tissue
and therapy response.
Methods: Ceramide (CER) and sphingomyelin (SM) serum
levels were analysed in 22 patients with locally advanced
rectal cancer and 24 healthy individuals by ultrafast liquid
chromatography coupled with tandem mass spectrometry.
The expression of pro-apoptotic BAX (BCL2 associated X,
apoptosis regulator) and anti-apoptotic BCL2 (BCL2 apoptosis regulator) was analysed in tumour and corresponding
healthy tissue samples of patients by quantitative real-time
PCR.
Results: Significantly lower serum levels of C18 CER, C22
CER, C24 CER, C18 SM and C24 SM were observed in
patients than in controls (P<0.05). For C20 CER, C22 CER
and C24 CER, a positive correlation with the pro-apoptotic
status of tumour tissue was found (r=0.619, P=0.018;
r=0.694, P=0.006 and r=0.601, P=0.023, respectively).
No difference in serum sphingolipid levels was found
between patients with good, moderate, and poor responses
to therapyUvod: Promene u nivou sfingolipida mogu da doprinesu nastanku karcinoma rektuma, progresiji bolesti i odgovoru na
terapiju usled njihove regulacije razli~itih biolo{kih pro cesa
uklju~uju}i apoptozu. Cilj ove studije je bio da se anali zira
profil sfingolipida u serumu kod pacijenata sa karcinomom
rektuma i da se ispita njegova povezanost sa apoptotskim
statusom tkiva tumora i odgovorom na terapiju.
Metode: Nivo ceramida (CER) i sfingomijelina (SM) je ana -
liziran u serumu kod 22 pacijenta sa lokalno uznapredova -
lim karcinomom rektuma i kod 24 zdrave osobe pomo}u
ultrabrze te~ne hromatografije kombinovane sa tandem
masenom spektrometrijom. Ekspresija pro-apoptotskog
gena BAX (BCL2 asocirjau}i X, regulator apoptoze) i antiapoptotskog gena BCL2 (BCL2 regulator apoptoze) je ana -
lizirana pomo}u metode kvantitativni PCR u realnom vremenu u uzorcima tumorskog tkiva i zdrave crevne sluznice
kod pacijenata.
Rezultati: Nivoi C18 CER, C22 CER, C24 CER, C18 SM i
C24 SM su bili zna~ajno ni`i u serumu pacijenata u odnosu
na kontrolnu grupu (P<0,05). Za C20 CER, C22 CER i
C24 CER je utvr|ena pozitivna korelacija sa pro-apoptotskim statusom tumorskog tkiva (r=0,619, P=0,018;
r=0,694, P=0,006 i r=0,601, P=0,023, respektivno).
Nije prona|ena razlika u nivoima sfingolipida u serumu
izme|u pacijenata sa dobrim, umerenim i lo{im odgovo -
rom na terapiju