Notulae Botanicae Horti Agrobotanici Cluj-Napoca
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Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities
Get PDFPrunus spinosa leaf extracts in solvents of different polarity (water, ethanol and acetone), their phenol, flavonoid and anthocyanin contents and biological properties were the object of this study. The richest in phenols as well as in flavonoids was acetone extract with 181.19 mg GAE and 80.10 mg QE per gram of dry extract, respectively. Moreover, the quantity of anthocyanins obtained by HPLC analysis was also the highest in acetone sample. Examined samples possessed antioxidant properties evaluated through four in vitro assays (DPPH, ABTS, FRAP and TRC). The acetone extract was proved to be the best antioxidant among tested samples, which could be ascribed to polyphenols, especially anthocyanins. The aqueous and the ethanol extract exhibited antibacterial effects, being particularly active against B. cereus and E. cloacae. T. viride, P. funiculosum, P. ochrochloron, P. verrucosum var. cyclopium were the most susceptible among fungal microorganisms examined. Both, the aqueous and the ethanol extract expressed inhibitory activity towards enzymes linked to diabetes mellitus type II. Additionally, the ethanol extract showed significantly higher potential in inhibiting α-glucosidase than the drug used as the positive control. Furthermore, the aqueous sample revealed antitumor effects on following malignant cell lines: HeLa, K562 and MDA-MB-453. The results presented herein suggest that P. spinosa leaves should be considered as a natural source of bioactive compounds with potential application in phytopharmacy and food industry
Biosorption efficacy of living and non-living algal cells of Microcystis aeruginosa to toxic metals
Get PDFThe existence of metallic elements in the aquatic environment is recognized to cause acute destruction to aqueous life. This study depicts the prospective application of cyanobacterial strains of Microcystis aeruginosa as a sorption material of toxic elements, aluminium (Al), and cadmium (Cd) from aqueous solutions. Algal samples were revealed to the metal solution, a noticeable modification change in cell wall structure surface occurred. The Fourier-transform infrared (FTIR) analysis illustrated the reality of carboxyl, carbonyl, and hydroxyl moieties, which are liable for the uptake of essential and nonessential elements aluminium and cadmium, respectively. The results showed the ability of Microcystis aeruginosa to uptake Al and Cd at the optimal temperature, light, and pH by living and non-living cells in the concentration of 20 ppm and stimulated antioxidant resistance against oxidative stress. This finding divulged that Microcystis could be utilized as an efficient bio-sorbent for the elimination of these ions, especially Cd from freshwater
Exogenous ABA foliar spray treatment alleviates flooding stress in adzuki bean (Vigna angularis) during the seedling stage
No full textDOI: 10.15835/nbha49412210
Abscisic acid (ABA) plays an important role in regulating activities of antioxidant enzymes, hormone levels, accumulation of reactive oxygen species under stress. To understand the effects of exogenous ABA treatment on flooding tolerance at seedling stage in adzuki bean (Vigna angularis), the water-sensitive (WS) variety ‘Tianjinhong’ (TJH) and the water-tolerant (WT) variety ‘Longxiaodou 4’ (LXD 4) were foliar sprayed exogenous ABA with 20 μM, followed by flooding stress for 5 days under pot conditions. Our results revealed that under flooding stress at seedling stage, the contents of H2O2 and MDA, proline and soluble protein in adzuki bean leaves significantly increased, the contents of ABA, IAA and SA significantly increased, and the activities of SOD, POD and CAT also significantly increased. Flooding treatment for 5 days resulted in a significant decrease in single pot yield of LXD 4 by 8.40% and TJH by 9.91%. Foliar spray of 20 μM exogenous ABA could resist flooding stress, considerably increased the contents of proline and soluble protein, significantly decreased the contents of H2O2 and MDA, significantly increased the activities of SOD and POD, increased the contents of ABA and SA, and significantly inhibited the increase of IAA content in adzuki bean leaves. Spraying exogenous ABA significantly increased the yield of ‘LXD’ 4 by 6.95% after 4 days of flooding treatment, and ‘TJH’ by 4.46%. To sum up, there were significant differences in physiological stress among different varieties of adzuki bean under flooding stress, and spraying exogenous ABA could effectively alleviate the effects of flooding stress on physiology and yield of adzuki bean. The results of this study provided a theoretical basis for further studying the physiological mechanism of adzuki bean resisting flooding stress at seedling stage and improving the yield of adzuki bean under flooding stress. Thus, foliar spraying exogenous ABA effectively improved submergence tolerance by enhancing the activity of protective enzymes and osmoregulation. These results provided novel insights and were expected to aid in the development of more effective stress resistance cultivation methods in adzuki bean production
Do nitrogen and zinc application alleviate the adverse effect of heat stress on wheat (Triticum aestivum L.)?
Get PDFLate-season heat stress (LSH) is a limiting factor for wheat production. Besides, low zinc and poor protein diet usually is a problem in low-income countries. The primary calorie source in such countries is prepared from bread. This study aims to mitigate heat stress by zinc and nitrogen application and improve zinc and protein content in wheat grain. We did the field experiments as a split-split-plot based on a randomized complete block design with four replications to assess zinc and nitrogen’s possible mitigation effect on LSH and protein and zinc enrichment of wheat grain during two years. Factors included LSH by delay in planting date (optimum, late, and very late) as the main plot, nitrogen (0, 75, 150, and 225 kg ha–1) in subplots, and zinc (0, 10, and 20 kg ha–1) as sub-subplots. We measured yield, yield components, physiological traits, zinc, and protein contents in the grain. Results showed that the highest relative water content and cell-membrane thermal stability were attained at the optimum planting date, 150 kg N ha–1 and 20 kg Zn ha–1. The maximum chlorophyll a and carotenoids contents in wheat cells were recorded in the optimum planting date, 225 kg N ha–1, and 20 kg Zn ha–1. Heat stress reduced the grain yield. In the second year of the experiment, the grain number per unit area was more than that of the first year; however, the highest grain yield was achieved in the first year owing to the higher mean grain weight. Nitrogen application decreased the adverse effects of heat stress on grain yield by increasing the grain number. Zinc application diminished the adverse effects of heat stress by increasing the mean grain weight. The adverse impact of the LSH on grain yield was more than that of biological yield. Heat stress reduced the hectolitre weight and zinc content of the grain. Meanwhile, it increased grain protein. In general, under LSH, the application of 225 kg N ha–1 and 20 kg Zn ha–1 can reduce the adverse effects of heat on the grain quality and quantity
Effect of digestate from anaerobic digestion on Sorghum bicolor L. production and circular economy
Get PDFThe aim of the research was determination the efficiency of application 50 t ha-1 digestate from the process of anaerobic digestion on the productivity of sorghum (Sorghum bicolor L.) on moderately degraded (calcic gleysol) and fertile (chernozem) agricultural land, in southern Banat, Republic of Serbia. In the field experiment during three years digestate amendment led to an increase in the number of leaves by 28.56% and plant height by 5.34%, which led to an increase in yield by 3.40%. The maximum yield was 2018 (41.74 DM t ha-1) on chernozem. The yield of sorghum was lower on calcic gleysol compared to chernozem by 5.43% and was in positive, medium and very significant dependence on precipitation (0.61) and in positive significant correlation with digestate (0.53) and plant height (0.59). Biogas yield reach 157.05 Nm3 t-1 (9582 Nm3 ha-1) on chernozem with digestate. Digestate had a statistically significant positive effect on all tested characteristics of sorghum as well as biogas yield during all three experimental years. The use of digestate as a by-product in the process of producing biogas based on silage of sorghum, allows the substitution of mineral fertilizers and remediation of damaged soil, which contributes to sustainability from the bio-economic and environmental aspects
Plant growth-promoting rhizobacteria associated to candelilla rhizosphere (Euphorbia antisyphilitica) and its effects on Arabidopsis thaliana seedlings
Get PDFIn the communities of Sierra Mojada and Viesca, Coahuila, Mexico of Coahuila desert, two rhizosphere samplings of candelilla (Euphorbia antisyphilitica Zucc) were collected to isolate, characterize, and identifying plant growth-promoting rhizobacteria (PGPR); 165 rhizobacteria were tested in vitro with Arabidopsis thaliana seedlings to evaluate their potential as plant growth promoters, and obtaining 21 strains with best results in the variables of the number of secondary roots and fresh weight concerning the uninoculated control. Their salinity tolerance was evaluated at concentrations from 0.85 M, 1.7 M and 2.55 M of NaCl. Biochemical tests were accomplishing such as siderophores production, phosphates solubilization, production of Indole-3-acetic acid (IAA), and the activity of the ACC deaminase enzyme. The results obtained from 21 strains selected, high activities were obtained in organic substances like a siderophores since they developed a translucent orange halo around their growth; four rhizobacteria developed a clear halo around the bacterial growth with a thickness between 1.487 mm ± 0.667 mm and 5.267 mm ± 0.704 mm in phosphates solubilization; in the production of Indole-3-acetic acid (IAA), the bacterial strains showed the presence of this phytohormone, with values from 4.444 μg mL-1 to 19.286 μg mL-1; and according to the activity of the ACC deaminase enzyme, values from 0.424 to 1.306 µmol α-KB/h/mg Pr were showed. 16S rRNA sequencing was carried out and genus identified were Bacillus, Staphylococcus, Acinetobacter, Cronobacter and Siccibacter. The results obtained show the potential of the isolated rhizobacteria as growth promoters and the increase in the biomass of the Arabidopsis thaliana seedlings is evident. This is a first indication to proceed to carry out tests in different phenological stages in crops of agricultural importance
Agronomical, physiological and molecular evaluation reveals superior salt-tolerance in bread wheat through salt-induced priming approach
Get PDFSalt stress significantly limit wheat crop productivity worldwide. Exposure to non-lethal levels of salt stress, referred to as "salt-priming", allows plants to persist subsequent lethal conditions; the priming effect continues even after an extended salt stress-free period. This study attempted to evaluate the effectiveness of the salt-induced priming approach to cope with the toxic effects of long-term salinity stress in wheat. After 22 days of gradual salt acclamation to reach 250 mM NaCl, plants were recovered for eight days and finally shocked with 250 mM NaCl (priming+shock) for 7 days. After that, physiological parameters and gene expression of six salt-responsive genes were assessed. Additionally, 120 days after germination (at the end of the season), agronomic traits were recorded. Analysis of the agronomical traits revealed higher productivity in the salt-pretreated group (priming+shock) plants than the non-pretreated (shock only). Consistently, salt-pretreated plants maintained higher photosynthetic pigments level and decreased proline and MDA content than non-pretreated, suggesting enhanced salt tolerance. Moreover, salt-pretreated plants sustained high expressional levels of salt-responsive genes (TaNHX1, TaSOS1, TaSOS4, TaHKT1, TaHKT2, and TaAKT1) comparing with non-pretreated, indicating a vital role in ion homeostasis and conferring salt tolerance. Ultimately, this finding could facilitate novel smart approaches to improve wheat productivity under salt stress
Alterations in leaf anatomy, quality, and quantity of flavonols and photosynthetic pigments in Nigella sativa L. subjected to drought and salinity stresses
Get PDFNigella sativa was widely used for nutritional and medicinal purposes. The present study investigated the effect of drought and salinity stresses on anatomical leaves structure and some biochemical properties to increase the secondary metabolites. For salt stress plants were treated with NaCl (30, 60 mM), and for drought stress plants were irrigated daily (control), once every two days (2DI) and once every three days (3DI). Compared to control plants, 2DI, 3DI, and NaCl 60 mM treatments increase significantly leaf rutin content, while the amount of rutin in seeds of NaCl 60 mM treated plants showed a significant decrease. 3DI treatment also significantly increased rutin content in seeds compared to NaCl 30 mM and control plants. The maximum level of quercetin (0.58 mg g−1 DW), kaempferol (0.16 mg g−1 DW), and myricetin (0.04 mg g−1 DW) in leaves were gained in both NaCl treatments. However, the flavonol components were affected more at salinity conditions rather than drought. In all treated plants, the amount of these compounds in leaves was more than in seeds. The highest amount of total phenol (130 mg g−1 DW), flavonoids (11.4 mg g−1 DW), and carotenoid content (1.55 mg g−1 DW) of leaves were observed under 2DI stress. Treated plants probably encountered different changes in the anatomical structure of leaves, including the decrease of phloem area, reducing vascular bundles and diameters, decreasing the number, and increasing the volume of cortex cells. The study also corroborates the cooperation between increasing the antioxidant capacity with the total flavonoid, rutin, and quercetin. Results indicated a higher sensitivity of N. sativa to drought stress than salinity stress and indicated that moderate salinity and drought could enhance secondary metabolites of seeds in this plant. The formation of potent antioxidants via the treatments could be worthy for pharmaceutical industries
The fundamental role of DELLA protein and regulatory mechanism during plant growth and development
Get PDFGibberellins (GAs) play a major role in a variety of key plant development processes, especially in promoting seed germination, stem and root growth, and fruit development. DELLA proteins are the core elements in GA signal transduction pathway, which exist in the plant nucleus and belong to the GRAS protein family. DELLA proteins negatively regulate the GA signaling pathway and biosynthesis, inhibiting plant growth. DELLA proteins can also interact with F-box, PIFS, ROS, SCLl3 and other proteins to enhance plant response to various adverse environmental influences such as drought, low and high temperature, heavy metal stresses. In addition, DELLA proteins can also partially regulate plant growth and development through interacting plant hormones such as ABA (abscisic acid), CK (cytokinin), ET (ethylene), BR (brassinosteroid) and JA (jasmine). This review summarized the basic characteristics of DELLA proteins, the transduction of hormone and environmental signals, as well as the regulation of plant growth and developments. DELLA proteins have broad application prospects in modern agricultural production in the future, but the molecular mechanism of DELLA proteins regulating plant growth and development are still unclear, and needs further study
Zoophagous entomofauna and entomopathogenic agents reported on Cydalima perspectalis (Walker, 1859) in north-western of Romania
Get PDFIn Europe, the box tree moth, Cydalima perspectalis (Walker, 1859) was first reported in 2006, in southwestern Germany, then spread to almost all countries. Larvae of this species affect the aesthetic value of the box tree. In the last period, numerous studies were carried out regarding the identification of useful entomofauna that reduce the numerical density of the population of Cydalima perspectalis. In this study conducted in north-western Romania (Cluj County, in 2019), entomopathogens and useful entomofauna were monitored in four areas, represented by four cities. The entomopathogenic agents contaminated the larvae of the box tree moth in a proportion of 5.6% at Gherla, 6.8% at Dej, 8.7% at Cluj-Napoca and 15.3% at Ciucea. Most larvae have been infected with Bacillus. At Cluj-Napoca and Ciucea, the presence of the larval endoparasite Exorista larvarum L. (Diptera: Tachinidae) was reported. Parasitoid species determined a parasitization rate of 5.1% at Dej, 8.6% at Gherla and 13.4% at Cluj-Napoca. Predators affected pupae in a proportion of 3.8% at Gherla, 4.2% at Dej and 16% at Cluj-Napoca. Among the pupae predators, for the first-time common earwig was reported, Forficula auricularia L. Entomopathogenic agents affected 5.3% of the pupae collected from Cluj-Napoca, 7.7% in Gherla and 12.5% in Dej. During the monitoring period of the zoophagous entomofauna and the entomopathogenic microorganisms, affected the pupae in a percentage of 20.1% in Gherla, 21.8% in Dej, 34.7% in Cluj-Napoca, contributing to the diminution of the population of the harmful species