Notulae Botanicae Horti Agrobotanici Cluj-Napoca
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    Effect of grape yield and maceration time on phenolic composition of ‘Fetească neagră’ organic wine

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    The phenolic composition of wine is mostly determined by the accumulation of the phenolic compounds in the grapes, as well as their extraction into wine. To increase their concentration in grapes, yield reduction is usually performed by pruning, while to increase the extraction in wines, the maceration on skins is extended for longer periods of time. The present study focuses on the possibilities to apply both strategies to improve the polyphenol composition of organic red wines of Romanian variety ‘Fetească neagră’, which stands to benefit more from technological interventions than other varieties, which naturally accumulate higher phenol concentrations in the grapes. In the vineyard three experimental pruning variants were made, with 20, 28 and 36 buds/vine, while for wine, maceration was performed for either 8 or 16 days for each grape variant. The phenolic profiles of wines were determined by HPLC methods. The main anthocyanidins, such as malvidin, petunidin, delphinidin, peonidin and cyanidin, as well as the acylated and coumaroylated derivatives of malvidin and peonidin were quantitatively determined. Some other phenolic compounds, of various classes, such as gallic, p-benzoic, p-coumaric and ferulic acid, catechin, epicatechin, myricetin, quercetin and trans-resveratrol were also determined. The quality of the organic ‘Fetească neagră’ wines depended highly on the vintage, but yield reduction and the extension of skin maceration duration were especially beneficial in the less favourable year, when classical technologies lead to less accumulation of sugars, colour and other polyphenols. Concomitant application of both strategies led to the best results, irrespective of the year

    Exploring mycorrhizal fungi in walnut with a focus on physiological roles

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    Walnuts are an economically important forest tree used for timber and nut production, and the nut of fruits is rich in various nutrients, becoming one of the four important nuts in the world. Walnuts have deep roots, which can be colonized by either ectomycorrhizal fungi or arbuscular mycorrhizal fungi in the soil. These mycorrhizal fungi form beneficial symbioses in roots of walnut. A large number of ectomycorrhizal fungi have been identified, whilst Boletus edulis, Calvatia uiacina, and Cantharelles cibarius isolated from walnut orchards stimulated plant growth and gave the capacity of stress tolerance in walnut. Moreover, Carya illinoensis is a very good host plant for commercial production of truffles, especially Tuber indicum. In addition, ectomycorrhizal fungi accelerate plant growth and enhance potential stress tolerance of walnuts. Inoculation with arbuscular mycorrhizal fungi also showed the improvement of plant growth and nutrient acquisition of walnut, the enhancement of drought tolerance in walnut, nutrient redistribution under walnut interplanting patterns, and the delivery of juglone by mycorrhizal hyphae. A culturable in vitro arbuscular mycorrhizal like fungus Piriformospora indica also enhanced salt tolerance of walnut plants. In this mini-review, the physiological roles of mycorrhizal fungi, including arbuscular mycorrhizal fungi, ectomycorrhizal fungi and arbuscular mycorrhizal like fungus (P. indica) on walnut plants are summarized, and future outlooks in the field are proposed

    Detecting trends in the quality and productivity of grasslands by analyzing the historical vegetation relevés: A case study from Southeastern Carpathians, Vlădeasa Mountains (Romania)

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    Grassland ecosystems are essential for biomass production but are prone to degradation if management practices are inappropriate. Thus, it is necessary to optimize grazing management since the grazing practices and grassland status are interconnected. Herbage mass production and forage quality of the plant species are among the most important factors for grazing livestock performance, grassland carrying capacity, and their sustainable management. We employed optimized methods for the analysis of two historical vegetation datasets (from 1970 and 2008), along with the statistical data on livestock numbers and types from three administrative units within the Vlădeasa Mountains area, in the Romanian Carpathians. We looked for trends in grassland quality and productivity and explored their connections to grazing management descriptors and practices. We identified a small but statistically significant decreasing trend between the two periods in both pastoral value (from 63.80 to 61.43) and productivity (from 10.80 t ha-1 to 9.18 t ha-1). The decline in grassland quality and productivity may be associated with the sharp decrease in livestock numbers (from 9,688 LU to 5,085 LU) and the replacement of cattle by sheep as the dominant livestock type. The abandonment of grasslands and traditional practices also increased the deviation from the optimum of the actual stocking rate. This approach can be used as a model for other areas where time-series vegetation data are available from phytosociological literature and/or databases. These insights can be used to design adaptive grazing management plans to optimize grazing management according to the carrying capacity of the grassland ecosystems

    Genome-wide investigation of Hydroxycinnamoyl CoA: Shikimate Hydroxycinnamoyl Transferase (HCT) gene family in Carthamus tinctorius L.

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    Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) is mainly associated with monolignol biosynthesis, a central precursor to producing guaiacyl and syringyl lignins in plants. However, the explicit regulatory mechanism of HCT-mediated monolignol biosynthesis in plants still remained unclear. Here, the genome-wide analysis of the HCT gene family in Carthamus tinctorius as a target for understanding growth, development, and stress-responsive mechanisms was investigated. A total of 82 CtHCT genes were identified and characterized. Most of the CtHCTs proteins demonstrated the presence of two common conserved domains, including HXXXD and DFGWG. In addition, the conserved structure of protein motifs, PPI network, cis-regulatory units, and gene structure analysis demonstrated several genetic determinants reflecting the wide range of functional diversity of CtHCT-encoding genes. The observed expression analysis of CtHCT genes in different flowering stages under normal conditions partially highlighted their putative roles in plant growth and development pathways. Moreover, CtHCT genes appeared to be associated with abiotic stress responses as validated by the expression profiling in various flowering phases under light irradiation and MeJA treatment. Altogether, these findings provide new insights into identifying crucial molecular targets associated with plant growth and development and present practical information for understanding abiotic stress-responsive mechanisms in plants

    Incidence of 14 grapevine viruses in Korean vineyards

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    DOI: 10.15835/nbha49412490 The incidence of grapevine virus infections in Korean vineyards was investigated from July to October, 2020. A total of 177 petiole samples were collected from two or three different cultivars in each of four different regions; these were examined by reverse transcription-polymerase chain reaction assay for the presence of 14 major viruses. The overall occurrence of grapevine viruses was 91.0%, and the level of incidence was high irrespective of region or cultivar. The predominant viruses were grapevine leafroll-associated virus 3 (80.2%), grapevine fleck virus (70.6%), and grapevine rupestris stem pitting-associated virus (49.2%). Most grapevines were infected with multiple viruses, suggesting that Korean vineyards are likely to suffer economic losses resulting from viral diseases. This is the first extensive survey performed in Korea to observe the outbreak status of diverse grapevine viruses; surveys of this type can provide important information for the management of grapevine viruses in Korea

    A new methodological contribution in mapping Romanian flora

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    In dem vorliegenden Beitrag wird der methodologishe rahmen zur Kartierung der Flora Rumaniens, am Hand des im jahre 1969 seitens der Auteren veroffentlichten Projekts erweitert. Unter der Akzeptierung des Netzverharens, indem die Karte in konischer Projektien angewendet wurde, ist der Zwischenraum von 1grad Langengrad in 8 gleiche Teile eingeteilt wegegen, jades interval von 1 grad Breitengrad in 12 gleiche Teile untergeteilt ist, woraus sich 96 elementare Vierecke ergeben, die durch die genauen geographischen Koordonaten auf der Flache von 1grad Breitengrad/1grad Langengrad bestimmbar sind. Am sudlichen Kartenrand wurden die Kolonnen von 1grad von Westen gegen Osten mit dem Buchstaben des Alphabets (A-J) bezeichnet, wahrend die horizontalen Reihen von 1grad auf dem ostlichen Kartenrand die romischen Ziffern (I-VI) fuhren (Fig. 1-3). Im Hauptviereck mit einer Flache von 1 grad Breitengrad/1 grad Langengrad sind die elementare Vierecke forlaufend von 1-96 nummerotiert, aber auch entsprechend mit einer mittleren Viereckgrosse gruppiert, mit den Buchstaben "a-f" vermerkt, nach dem Modell aus der nord-ostlichen Ecke der Abbildung 1-3

    Chorology and phytocenological integration of Adonis volgensis in R. S. Romania

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    In continuation of former cytological studies (1972) the paper presents phytochronological and phytocenological data regarding Adonis volgensis Stev. and A. hybrida Wolff from Romania, with special reference on an isolated point of the disjunct distribution of the species (Transylvania, Cluj, finatele Clujului, Fig. 1. D.III.22.; Fig. 2. D.III.22 12-13 and 22-23). At this point, on a restricted territory of about 1000 m2, a small population of A.volgensis can be found, which presents complex microevolutive phenomena as a result of introgresiv hybridization with a vastly out numbering population of A. vernalis L. The habitat of the species is steadily endangerd by herborizers and by rapid extansion of the city of Cluj. For the chorological representation of data the mapping system elaborated by A. Nyarady and E. C. Vicol (1973) have been applied, for the phytocenological processing the principles and symbolization of Central-European phytocenological school have been followed

    Aloe vera gel microcapsules and essential oils of thyme and oregano incorporated in spreadable goat cheese: impact on its microbiological, physicochemical, and sensory characteristics during storage

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    The aim of this study was to develop a new assortment of high-quality cream cheese, with a high amount of biologically active compounds, namely a spreadable cheese from goat milk with addition of Aloe vera microcapsules and essential oils of thyme and oregano. The research was focused on the testing and optimization of an appropriate manufacturing recipe for this new product. Two assortments were made: one with Aloe vera microcapsules and thyme essential oil and the other one with Aloe vera microcapsules and oregano essential oil. For each assortment two different concentrations of Aloe vera and essential oils were tested. The obtained products were analysed for organoleptic, physico-chemical (fat, protein, total dry matter, sodium chloride and acidity) and microbiological parameters. In terms of consumer preferences, the cream cheese with 3% microcapsules of Aloe vera gel and 0.018% thyme essential oil was the most appreciated in terms of commercial aspect, consistency, and taste. We concluded that the addition of Aloe vera microcapsules improves the sensory characteristics and leads to an increase nutritional value of the finished product. Also, the addition of essential oils (thyme and oregano) ensured a stable quality during storage

    Water-use efficiency and nitrogen uptake in rice seedlings grown under different light quality

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    Rice (Oryza sativa L.) cultivars ‘Taichung shen 10’ (‘TCS10’) and ‘IR1552’ were hydroponically grown under different light conditions to investigate the effect of light quality on their biomass, transpiration, water-use efficiency (WUE), carbon isotope discrimination (Δ), seed nitrogen (N) contribution and nitrogen uptake ability from the hydroponic nutrient solutions. Light emitting diode (LED) lighting systems were used to control light quality. Different light treatments were applied to the rice seedlings including red (R), green (G), and blue light (B), with red + blue light (RB) as control. The photon flux density was set at 105 μmol m-2 s-1. WUE and Δ were combined to evaluate whole-plant WUE. Improved whole-plant WUE was observed for both cultivars under R and RB light due to lower transpiration rates than under B light. Green light also improved Δ in both rice seedling cultivars. Seed N contribution of both cultivars was stable across all light treatments, while improved N uptake ability was observed under B and RB light. In addition, N uptake in ‘IR1552’ rice seedling cultivars did not respond as favourably to green light as ‘TCS10’ cultivars

    Canopy analysis and thermographic abnormalities determination possibilities of olive trees by using data mining algorithms

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    In order to take the appropriate tree protection measures, it is crucial to determine and track abnormalities that may occur in olive trees in time to time for many reasons. Abnormalities start in different sections of the trees, depending on the environmental effects of the olive tree, with a specific impact like fungal diseases, drought, etc. after a certain age especially in non-resistant species. Protection steps may be taken when abnormalities are apparent or predictable in certain olive trees, using some external indicators. However, when abnormalities formed within trees cannot be identified externally, there is a sudden breakdown and overthrow of valuable properties, such as monument trees. In the literature, various devices and methods are explained to classify these defects in different trees. By the way, in this research, a non-destructive inspection method (thermography) was clarified and used to assess anomalies in old olive trees without damage in the interior. According to the results of average thermal data, 60, 400, 600 year-old olive trees, 60-40, 70-30 and 80-20 learning-prediction data rates decision tree and random forest results according to normal and abnormal thermal difference, the thermal range was found as 35.95 ℃ at 60 year-old tree, also it was found as 36.25 ℃ at 400 year-old tree and it was found as 38.25 ℃ at 600 year-old tree

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