Notulae Botanicae Horti Agrobotanici Cluj-Napoca
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Assessment of common wheat (Triticum aestivum L.) yield and quality under organic farming in the southwest of Romania
Taking into account the rising demand for organic wheat and the need to guarantee both high productivity and quality of the crops, the choice of cultivar plays a crucial role. These intended objectives may solely be attained by employing the most appropriate cultivars for organic agriculture. The aim of this study was to evaluate the suitability of 15 wheat cultivars for cultivation in organic farming according to three important parameters for organic farmers and processors, namely grain yield, test weight and protein content. The field experiment took place over two consecutive growing seasons, in the center of the Oltenia region (southwest of Romania) under organic farming conditions. The results showed significant effects of the climatic conditions in the year of study, but also of the cultivar on all studied parameters. The effect of genotype-environment interactions was significant only for test weight and protein content. On average, over the two years of study, the grain yield was 3899 kg ha-1, the test weight was 70.8 kg hl-1, and the protein content was 10.1%. The recently released cultivar ‘Voinic’ obtained the maximum grain yield (4755 kg ha-1) and also a maximum test weight and protein content (73.9 kg hl-1, respectively 10.7%). In conclusion, it follows that by using cultivars suitable for organic farming, good and quality yields can be obtained that can be a suitable raw material for baking industry
Transcriptomic analysis reveals transcription factors involved in vascular bundle development and tissue maturation in ginger rhizomes (Zingiber officinale Roscoe)
Ginger (Zingiber officinale Roscoe) is an important vegetable with medicinal value. Rhizome development determines ginger yield and quality. However, little information is available about the molecular features underlying rhizome expansion and maturation. In this study, we investigated anatomy characteristics, lignin accumulation and transcriptome profiles during rhizome development. In young rhizomes, the vascular bundle (VB) was generated with only vessels in it, whereas in matured rhizomes, three to five layers of fibre bundle in the xylem were formed, resulting in VB enlargement. It indicates VB development favouring rhizome swelling. With rhizome matured, the lignin content was remarkably elevated, thus facilitating tissue lignification. To explore the regulators for rhizome development, nine libraries including ginger young rhizomes (GYR), growing rhizomes (GGR), and matured rhizomes (GMR) were established for RNA-Seq, a total of 1264 transcription factors (TFs) were identified. Among them, 35, 116, and 14 differentially expressed TFs were obtained between GYR and GGR, GYR and GMR, and GGR and GMR, respectively. These TFs were further divided into three categories. Among them, three ZobHLHs (homologs of Arabidopsis LHW and AtbHLH096) as well as one DIVARICATA homolog in ginger might play crucial roles in controlling VB development. Four ZoWRKYs and two ZoNACs might be potential regulators associated with rhizome maturation. Three ZoAP2/ERFs and one ZoARF might participate in rhizome development via hormone signalling. This result provides a molecular basis for rhizome expansion and maturation in ginger
Efficient utilization of pine logs in northern Mexico through training
The timber industry and other sectors that rely on forest management, would benefit from a deeper understanding of the extraction work carried out by properly trained workers. The objective of this research was to evaluate the effect of training operational personnel on the efficient utilization of harvested pine logs in the southern region of the state of Chihuahua, in northern Mexico. Two samplings of the logging present in the cutting area of the ejido Basihuare (communal land) were carried out, taking into consideration the diameter with and without bark, visual quality, length, and use for reinforcement. The sampling involved recording 116 logs prior to a directional felling and log dimensioning training process, and 336 logs divided into three sampling blocks after the training. The data was analysed with tests for variance homogeneity, and ANOVA analyses were performed at a significance level of 0.05. It was found that training forestry harvest personnel allows for an improvement in the process of classifying logs destined for industrial use according to their dimension and visual quality. Additionally, the training allowed for the proper adjustment of reinforcements applied in the log dimensioning process, and as a result, it was possible to efficiently utilize available resources, significantly reducing the generation of waste after resizing of the wood products. For the advancement of the forestry sector, it is necessary to prioritize training as a driving element of quality to raise operational efficiency parameters of production and, consequently, promote the evolution of the production process
The relationship between habitat factors and the nutrient contents of wild Allium victorialis L. in the Changbai Mountains
Allium victorialis L. (Family: Liliaceae) is an herb with nutritional and medicinal properties. In Jilin Province, China, A. victorialis is mainly distributed in the Changbai Mountains and grows in various habitat conditions. However, the relationship between habitat factors and the nutritional quality of A. victorialis in the Changbai Mountains has not yet been examined. We assessed the nutritional quality of five A. victorialis populations growing in five different habitats in the Changbai Mountains and analyzed the relationship between nutritional quality and habitat factors. Allium victorialis populations in this region were primarily found in the undergrowth at elevations above 500 m and within specific ranges of air temperature, air relative humidity, soil temperature, and soil water content. Among the habitat factors investigated, canopy density significantly affected several nutritional components of A. victorialis; however, elevation had a significant effect only on the total flavonoid content, and the vitamin C content was not strongly associated with the main habitat factors in this study. During germplasm selection and artificial cultivation, it is important to simulate the growth conditions of the original habitat. Our results provide useful information for site selection and environmental condition optimization for the artificial cultivation of A. victorialis
Long-term experiments with fertilizers-essential fertility changes
The paper presents soil analytical data and their interpretation from samples in long-term experiments as a result of NP, NPK and organo-mineral fertiliser applications in a stationary system for 20 years and wheat-maize-soybean rotation system. The results show that NP fertilisation (from ammonium nitrate and superphosphate) leads to acidification of soils (regardless of soil type) depending on increasing N a.i./ha. The acidification phenomenon is higher in typical preluvosol (taxonomically acidic soil) due to activation of adsorbed (potential) acidity and solubilisation of Al ions, with devolatilisation of adsorbent complex, which updates for this soil the need for correction of the reaction (pH) by means of calcium amendments. In contrast, for the alluvial mollisol, with neutral - weakly alkaline pH and higher humus % and high buffering capacity, the multiannual acidification due to N is reduced. NP, NPK mineral fertilisation, in balanced doses, can maintain the organic-C and humus content constant, balanced, within the specific limits of the soil type over a period of 20 years, with wheat-maize-soybean crop rotation. In contrast, amendment and processing conventional intensive tillage (on maize) decrease the content of these indicators, a phenomenon that can be attributed to the enhanced mineralisation of the soil organic component. Organic and organo-mineral fertilisation can lead to a favourable modelling of humus content. The mobile forms of the essential elements of nutrition and fertilisation (N, P, K) are improved in terms of their bioavailability with the objective that these forms will maintain their quantity and quality at the level required by crops and their supply by fertilisation technologies is ensured rationally and preventively for the soil-plant system. In summary, the analytical results from long-term experiments with fertilisers are of direct benefit to soils, their productivity and fertility, with the implementation of sustainability principles
Physiological traits and metabolic profiles of contrasting rice cultivars under mild salinity stress during the seedling stage
This study aimed to investigate the effects of mild salinity stress on some physiological traits and metabolic profiles of rice during the seedling stage. Three rice cultivars differing in salinity stress response, ‘KDML105’ (salt-sensitive, local Thai jasmine rice), ‘IR29’ (salt-sensitive rice), and ‘Pokkali’ (salt-tolerant rice), were subjected to mild salinity stress by flood-irrigated with 40 mM NaCl. Salinity stress significantly reduces the growth and biomass of the sensitive cultivars. Electrolyte leakage in ‘KDML105’ and ‘IR29’ also increased significantly while the relative water content (RWC) decreased. However, salinity stress did not affect growth, electrolyte leakage, and RWC in the tolerant cultivar. Salinity significantly increased Na+/K+ ratio in shoot and root of all cultivars. Nevertheless, the increase of Na+/K+ ratio in ‘KDML105’ and ‘IR29’ was higher than that of ‘Pokkali’. The metabolic profile analysis of ‘KDML105’ showed that uracil, L-proline, malonate, L-carnitine, L-lactate, and guanine were significantly increased, but the levels of dihydroxyacetone and glycine were significantly decreased under salinity conditions. In ‘IR29’, L-proline, melatonin, gamma-aminobutyrate, and acetate were significantly increased. In ‘Pokkali’, however, sugar alcohols and sugars were slightly increased when exposed to salinity conditions. The metabolic pathway analysis revealed one involved metabolic pathway of ‘KDML105’: aminoacyl-tRNA biosynthesis, and three involved those of ‘IR29’: arginine proline metabolism, sulfur metabolism, and butanoate metabolism. The difference in the metabolic profile of the salt-tolerance salt-sensitive cultivars provides new insights to identify additional novel biomarkers that will help better understand the mechanisms for salt tolerance in rice
Drought stress-induced modification of morpho-anatomical and yield attributes of mung bean associated with the application of silicon and Moringa leaf extract
Mung bean (Vigna radiata) is the rich source of fiber and essential nutrients. They play a vital role in sustainable agriculture due to their ability to fix nitrogen in the soil and enhance soil fertility. Drought is characterized by limited water resources and severe arid climatic conditions, notably impair crop growth and yield. In the current experiment, two genotypes, Azri-M 2006 and NM-92, were studied against drought stress that was applied as 2 days and 4 days irrigation gap per week. Foliar application of magnesium-silicate (20 ppm and 30 ppm concentrations) and Moringa leaf extract (30% v/v solution) was applied as treatments. The results from the experiment morphology anatomical and yield components were recorded according to the prescribed methods. The result revealed that drought stress reduced the growth of plant. Foliar application of 30 ppm silicon against drought stress showed a highly significant (p<0.001) result compared with control group. Morphology parameters, including shoot and root length, shoot and root fresh weight, root dry weight, leaf area, leaf number, the anatomical structure included (stem epidermis, cortex, and stem vascular bundles,) and also yield components (pod length, and seed numbers). In contrast, MLE (30%) showed a significant impact (p<0.01) on leaf lamina thickness (Leaf anatomical parameters; midrib xylem and phloem, number of stomata on the adaxial and abaxial surface) and yield components included (100-grain weight, grains weight per plant, and numbers of pods,). The overall impact of 30 ppm Si was 39.9% more positive on Azri-M2006 than the NM-92 against the drought stress. The 30-ppm silicon and 30% MLE showed 90% similar results in all studied parameters. This study confirms that 30% MLE could be recommended to farmers to improve productivity under arid conditions than the silicon
Role of silica nanoparticles in enhancing drought tolerance of cereal crops
Cereal crops are essential for providing essential nutrients and energy in the daily human diet. Additionally, they have a crucial role as a significant constituent of cattle feed, hence enhancing meat production. Drought, being an abiotic stressor, adversely affects the growth and yield of numerous crops on a global scale. This issue poses a significant and pressing obstacle to maintaining global cereal crop production and ensuring food security. Nanoparticles have become a valuable resource for improving cereal crop yield and productivity under ongoing rapid climate change and escalating drought conditions. Among these, silica nanoparticles (SiNPs) have demonstrated their potential for agricultural applications in regions with limited water availability. Drought stress has detrimental effects on cereal crops, impacting their growth, metabolic, and physiological processes, hampering water and nutrient absorption, disrupting cellular membranes, damaging the photosynthetic apparatus, and reducing antioxidant activities by altering gene expression. SiNPs help preserve cellular membranes, regulate water balance, and improve water and nutrient absorption, resulting in a substantial enhancement in plant growth under water-deficit conditions. SiNPs also protect the photosynthetic system and enhance its efficiency, facilitate the accumulation of phenolics, hormones, osmolytes, antioxidant activities, and gene expression, thus empowering plants with increased resistance to drought stress. Moreover, SiNPs decrease leaf water loss by promoting stomatal closure, primarily by fostering the accumulation of abscisic acid (ABA) and mitigating oxidative stress damage by activating the antioxidant defence system and reducing reactive oxygen species (ROS). However, a limited number of studies examine the role of SiNPs in cereal crops under drought stress conditions. In this review, we highlighted the promising potential of SiNPs to improve cereal crop resilience by changing morpho-histological traits, antioxidant properties, and gene expression to maintain food security in drought-prone areas. This study will aid researchers in using SiNPs as an environmentally benign way to improving drought resistance in cereal crops in order to fulfill global food supply needs
Synergism of microorganisms and seaweed extract on vegetative growth, yield and quality of cucumber fruit
Natural biostimulants, such as microorganisms and seaweed extracts, are used in agriculture to improve crop yields with a sustainable approach. However, the interaction effects between different biostimulants have not been thoroughly investigated. The objective of this work was to evaluate the synergistic effects of microorganisms (Glomus intraradices and Azospirillum brasilense) and seaweed extract (Ascophyllum nodosum and Macrocytis pyrifera) on growth, yield and fruit quality of cucumber plants under soilless cultivation. Two doses of microorganisms (0 and 10 spores mL-1 + 106 CFU) and four concentrations of seaweed extract (0, 250, 500 and 2500 ppm) were evaluated. The experimental design used was a randomized complete block design with a factorial arrangement (2 x 4), with four replications per treatment. The results showed that the equatorial diameter of the fruit, yield and titratable acidity improve when applying microorganisms and seaweed extract in combination; however, when applying the two biostimulants the values of plant height, stem diameter, number of leaves, root length, biomass (fresh and dry), firmness, total soluble solids, vitamin C, chlorophyll (a and total) and indices of color (L* and b*) did not exceed those obtained when the biostimulants were applied individually. The combined application of microorganisms and seaweed extract improves cucumber yield, but not vegetative growth and, except for titratable acidity, fruit quality attributes
Biocontrol of the root-knot nematode Meloidogyne incognita with Purporeocillum lilacinum and liquid bio-formulates in tomato (Solanum lycopersicum)
Root-knot nematodes (Meloidogyne spp.) cause great losses in tomato crops. An environmentally friendly for its control is the use of predatory fungi such as Purporeocillum lilacinum, which reduces its population in the soil and mitigates yield losses. Therefore, the objective of the present study was to evaluate the biocontrol efficacy of the strains of the nematophagous fungus Purporeocillum lilacinum and liquid bioformulates on the eggs of the root-knot nematode Meloidogyne incognita, and the formation of galls on the tomato root. Two native strains of Purporeocillum lilacinum H2 and H3 combined with the bioformulated Extract of Beneficial Microorganisms (EPMB®) and the root exudate stimulator Exu-Root® were tested on Meloidogyne incognita eggs and tomato plants. The results obtained indicate that the Purporeocillum lilacinum H2 and H3 strains infected the Meloidogyne incognita eggs, interrupted the development of the embryos and caused their death, which significantly reduced the presence of galls in the root of the plants. In short, the maximum biological performance was presented with the treatments H2+Exu-Root® and H3+EPMB®, which had the lowest number of galls with 19.2 and 20.3 galls per plant respectively, compared to the control that presented 88 galls in the root and the treatments where the fungus was not applied (69-85 galls). Finally, it is concluded that the results demonstrate the potential of the Purporeocillum lilacinum H2 and H3 strains as biocontrol agents against the root-knot nematode Meloidogyne incognita, and that, in combination with EPMB® and Exu-Root®, the efficacy can be increased to reduce its population