Applied Science and Biotechnology Journal for Advanced Research
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Enhancing Medicinal Plants with Biotechnology
Get PDFThe majority of the world\u27s population gets its life-saving medications from medicinal plants, which are its primary source. High-quality plant-based medicines could be made via in vitro regeneration, which has a lot of potential. The essential genotypes of therapeutic plants must be chosen, multiplied, improved, analyzed, and conserved using biotechnological technologies. As microbial cells and chemical synthesis are unable to create useful therapeutic chemicals, flavors, perfumes, and colorants, plant cell culture methods represent a potential renewable source of these substances. Because in vitro propagation of medicinal plants with enhanced bioactive principles and cell culture methodologies for selective metabolite production are found to be extremely helpful for the commercial production of medicinally significant compounds, this review article discusses the role of biotechnology in the production of medicinal plants
The Newly Discovered Lactobacillus Plantarum JX183220 uses Cassava (Manihot Esculenta Krantz) Flour
Get PDFThe purpose of the current study was to use Response Surface Methodology to optimize the generation of lactic acid using a new isolate of Lactobacillus plantarum JX183220 and cassava flour (Manihot esculenta Crantz) in semi-solid fermentation.Methodology\u27s Box-Behnken study design was employed. Cassava flour (CF) was employed in a semi-solid fermentation with Lactobacillus plantarum JX183220, a bacteria isolated from goat milk. Initially, preliminary research adjusted a variety of fermentation parameters, including incubation time, inoculum volume, pH, temperature, substrate concentration (cassava flour), and calcium carbonate concentration. The response surface methodology\u27s Box-Behnken design was used to further optimize the substrate concentration, temperature, and pH as potential factors. The fitting of a second-order polynomial regression model, which had a high coefficient of determination, R2, was successful (0.9913). The validation experiment was conducted under the parameters\u27 ideal conditions as determined by the model. According to the preliminary research, Lactobacillus plantarum JX183220 produced the most lactic acid on the fourth day of incubation with 2% inoculum and 0.3% calcium carbonate. Maximum lactic acid production of 18.3679 g/100 g of cassava was achieved by optimization using the Box-Behnken design of RSM at the ideal substrate concentration, temperature, and pH conditions of 36.39°C and 6.43. A validation experiment supported these findings. The best conditions for a newly isolated strain of Lactobacillus plantarum JX183220 to directly convert cassava flour starch to lactic acid were identified. With 15 runs, the RSM\u27s Box Behnken design was determined to be an efficient instrument for maximizing the production of lactic acid. In the future, scientists may use fermentation and sugar-making to make even more lactic acid. 
Performance and Effect of Hybrid Rose Growth under Cultivars Plant Density
Get PDFTo ascertain the impact of cultivar, planting geometry, and their interaction on rose production and quality, a study was carried out in an open environment. The experiment was set up using a Randomized Complete Block Design (RCBD) with three replications in a 3 x 3 factorial configuration. The three cultivars (\u27Sophia\u27, \u27Gladiator\u27, and \u27Divine\u27) and three different planting densities (S1 (0.90 x 0.90 m), S2 (0.60 0.60 m) double row, and S3 (0.60 0.60 m) triple row) made up the treatments. Parameters relating to the growth, yield, and quality of Rose were the subject of data collection. According to the study\u27s findings, flower yield increased when plant density went from S1 (0.90 x 0.90 m) to S3 (0.60 x 0.60 m) triple row. On the other hand, intensive planting dramatically reduced the flower\u27s weight and shelf life. The cultivars "Sophia," "Gladiator," and "Divine" were found to be the most productive in terms of flower yield. The \u27Sophia\u27 and \u27Gladiator\u27 cultivars performed better than the \u27Divine\u27 cultivar in several of the characteristics. Commercial producers can employ the planting densities that showed the greatest and greatest beneficial effects on the yield and quality metrics, respectively. Further research is necessary, however, on matters relating to the estimation of nutrient supply for controlling the product\u27s fresh weight as well as other economic-related subjects
Enhancement of Proline and Hydroxyproline Metabolism and Heavy Metals Toxicity in Plant Life: A Study
Get PDFA significant amount of heavy metals have been added to the soil and water as a result of more anthropogenic and natural activity. Both humans and plants are much disturbed by these. As a result, there is a growing interest in the scientific study of the toxicity of heavy metals in plants. In this way, osmolytes, such as proline, are organic substances that typically control the sustenance and growth of plants that are commonly grown in the areas. Therefore, increasing the activity of various enzymes and metabolic pathways by adding proline or/by introducing gene(s) is a very effective strategy to deal with the toxicity of heavy metals. The specific function of proline in protecting against the toxicity of heavy metals has been briefly reviewed in this review
Farm Technology and Socio Economic Causes for Rural to Urban Migration in India
Get PDFThe act of accepting a village for some goal, such as development as in this situation, is known as "village adoption." At several levels in our nation, there are numerous village adoption initiatives being carried out. However, simply adopting them won\u27t be enough to build more than 6 lakh communities in India. Therefore, we must improve village adoption programmes in order to implement a model village approach, as village adoption must be seen as a key step in achieving this. The Model Village and Village Adoption Programmes are sometimes confused with one another, albeit they are not. This study makes an effort to evaluate these two strategies and offers special examples that illustrate the extent and potential of the Model village idea. With this innovative strategy, our nation\u27s progress is anticipated to accelerate
Analysis Protein Drugs Design Program with Quality and Structure Prediction
Get PDFA complete lack of hair will result when the "hairless protein" associated with the hairless gene, which is required for hair growth, ceases to function. The coordinates of this gene are 22027873-22045326 on chromosome 8. The JmjC domain superfamily includes the hairless gene, which also contributes to the process of histone demethylation. The domain sequence is 212 amino acids long and spans residue positions 946 to 1157 in the hairless protein, which has 1189 residues. JmjC domains have been discovered in more than 100 eukaryotic and bacterial sequences based on considerable sequence similarity, including the human hairless gene, which is mutated in people with alopecia universalis. To homology model the JmjC domain in the hairless protein, we have tried the bioinformatics method. NCBI-BLASTP, EBIClustalW, SMART, 3D-PSSM, DeepView/Swiss-PDB Viewer, PyMOL, and WhatCheck are the tools and programs utilized in this work. Using the template crystal structure of the likely antibiotic production protein from Thermus thermophilus HB8, the structure of the JmjC domain is predicted. Modeled domain structure\u27s minimized energy value was -3394.570 KJ/mol. The simulated domain structure was validated using the WHAT IF-Proteins Model Check tool
Preparation of Microwave Induced Ce4+ Substitution Properties of Chemically Substituted Zinc Ferrite Nanocrystals
Get PDFSol-gel auto combustion was used to create cerium doped Ni-Cr-Fe spinel ferrite nanoparticles. The cubic spinel structure with co-existence of the CeO2 and Fe2O3 phases is revealed by the XRD patterns of the samples sintered at 600 0C. When Ce4+ ions are added to nickel ferrites, the average lattice constant increases from 8.244 to 8.354 as the crystallite size increases. The well-defined and primarily spherical-shaped grains on the samples\u27 surfaces are visible in SEM micrographs. All of the materials\u27 infrared spectra were captured at room temperature in the 300–800 cm-1 range. The infrared spectra of the current samples also exhibit two major absorption bands, which is a characteristic of spinel ferrites. As the cerium content of Ni-Cr ferrite rises, saturation magnetization (MS) and remnant magnetization (Mr) decline
Heavy Metals and Zinc Levels Roles in the Environment and their use on Higher Plants
Get PDFNatural elements with a high atomic weight and a density greater than five times that of water are known as heavy metals. They are widely disseminated in the environment due to their numerous industrial, household, agricultural, medical, and technical applications, raising worries about their potential effects on both human health and the environment. The dosage, method of exposure, chemical species, as well as the exposed person\u27s age, gender, heredity, and nutritional status, all have an impact on how dangerous these compounds are. Arsenic, cadmium, chromium, lead, and mercury are among the priority metals of public health concern due to their severe toxicity. These metallic substances are regarded as systemic toxins that can harm different organs even at low exposure levels. The International Agency for Research on Cancer has classed them as human carcinogens as well. This study examines the prevalence, production, and usage of these chemicals in the environment as well as the possibility of human exposure and the molecular mechanisms of toxicity, genotoxicity, and carcinogenicity
Genetic Factors for Periodontal Diseases and Twin Model
Get PDFIt appears that periodontal disease is a collection of illnesses with overlapping symptomatology rather than a single illness with varying clinical signs. Periodontal diseases may have a complex character. Given that many risk factors for periodontal disease tend to cluster in families for genetic or cultural reasons, it will be crucial to take these factors into account when researching familial clustering or potential genetic causes. Finding potential genes that could be the cause of a person\u27s genetic vulnerability to periodontal disease will be crucial. The most obvious candidate genes are those that may influence how the immune system reacts to oral bacteria, but further study of disease-predisposing features, such as those related to tissue response, may reveal new hints regarding potential candidate genes. Clinical professionals may be better equipped to identify high-risk individuals for specialized prevention and treatment if these genes are discovered. The majority of the time, the existence of a number of environmental risk factors combined with a number of susceptibility variables during a specific stage of life is what most often leads to periodontitis developing in a person. The stronger the genetic predisposition and the greater the risk for early periodontitis development, the more susceptibility factors a person has inherited. As our understanding of the genes responsible for both major and modifiable diseases grows, numerous genetic tests may be developed. Medline, the Cochrane Database of Systematic Reviews, and DARE were used to compile the database. The database was compiled over the last 25 years, taking into account all developments and new evidence that occurred during that time. Running a preliminary search and noting the terms used in the titles and abstracts as well as the subject headers helped make the search more sensitive. The key concept was kept in mind, and synonym terms were searched using MeSH headings. By adhering to the inclusion and exclusion criteria for selecting articles, search results were narrowed down to those that supplied information and support for both periodontitis and genetics. The only articles left out were those that covered genetics in general
Study of Social-Ecological Systems and Climate-Related Disasters in the Indian Sundarbans
Get PDFThe interactions or connections between an area\u27s social system and ecological system are referred to as the "socio-ecological system" (SES). The Indian Sundarbans, which can be viewed as an integrated SES, are home to more than 5 million people and are rich in wildlife. The current study examines the aforementioned SES at the microlevel. In order to perform a primary survey using an appropriate questionnaire, four villages from two blocks of the Indian Sundarbans—Madhya Gurguria, Debipur, Satjelia, and Rangabelia—were chosen. The study\u27s findings indicate that the region is heavily dependent on its cattle, fishing, and agricultural resources. In the village economy, relying on numerous resources to generate revenue is the norm. However, this in turn is putting further strain on the already limited resource base. In order to analyse resource usage and flow at the microlevel in the Indian Sundarbans, the article does so. In order to secure the sustainability of both resources and the way of life of those who live in the SES of the Indian Sundarbans, the present study makes the case for the need of a strong framework that supports the inclusive and participatory participation of all stakeholders in resource management