CR Biologies

Claude Monneret

What is an endocrine disruptor?

Patrick Balaguer

Vanessa Delfosse

Marina Grimaldi

William Bourguet

Structural and functional evidences for the interactions between nuclear hormone receptors and endocrine disruptors at low doses

Suchada Sumruayphol

Boonruam Chittsamart

Raxsina Polseela

Patchara Sriwichai

Yudthana Samung

Chamnarn Apiwathnasorn

Jean-Pierre Dujardin

Wing geometry of Phlebotomus stantoni and Sergentomyia hodgsoni from different geographical locations in Thailand

CV2 - Editorial Board/Français

Sommaire / Contents

Mohamed Toumi

Selma Barris

Mohamed Seghiri

Houmam Cheriguene

Fatiha Aid

Effet de plusieurs méthodes de scarification et du stress osmotique sur la germination des graines de Robinia pseudoacacia L.

Rosaria Scudiero

Mariailaria Verderame

Gene expression profile of estrogen receptors alpha and beta in rat brain during aging and following high fat diet

Hani Amir Aouissi

Julien Gasparini

Adnène Ibrahim Belabed

Zihad Bouslama

Impact of greenspaces in city on avian species richness and abundance in Northern Africa

Rafael N. Carvalho

Adriano B. Kury

A new species of Discocyrtanus from Mato Grosso, Brazil (Opiliones: Gonyleptidae) with a key to the species of the genus

Xiaolong Wang

Lin Li

Jiaxin Zhao

Fangliang Li

Wei Guo

Xia Chen

Effets de différentes méthodes de préservation sur la répétition de séquence simple inter (ISSR) et Polymorphisme amplifié aléatoire DNA (RAPD) des marqueurs moléculaires dans les échantillons de botanique
Effects of Different Preservation Methods on Inter Simple Sequence Repeat (ISSR) and Random Amplified Polymorphic DNA (RAPD) Molecular Markers in Botanic Samples
Xiaolong Wang1, 2, Lin Li1, 2, Jiaxin Zhao1, 2, Fangliang Li1, 3, Wei Guo4, Xia Chen1, 2 *
National & Local United Engineering Laboratory for Chinese Herbal Medicine Breeding and Cultivation, School of Life Sciences, Jilin University, Changchun, Jilin province,130012, People’s Republic of China.
School of Life Sciences, Jilin University, Changchun, Jilin province,130012, People’s Republic of China.
Liaoning Technical University College of Science, Fuxin, Liaoning province, 123000, People’s Republic of China.
Changbai Mountain Academy of Sciences, Erdao, Antu, Jilin province,133613, People’s Republic of China.
* Corresponding author
E-mail: chenxiajlu@163.com 

Table S1 The primers we have selected and the annealing temperature (℃) for ISSR.
primers	T	C	H	S	Y	L
807	--	52.8	51.4	51.4	51.4	51.4
811	--	49.3	54.3	51.4	54.3	57.2
826	50.2	--	--	--	49	51.4
827	56.5	56.5	54.3	56.5	56.5	56.5
836	56.9	56.9	56.9	56.9 	56.9	56.9
842	50.2	59.8	59.8	59.8	59.8	59.8
851	53.2	59.8	53.2	54.3	49.3	59.8
856	--	56.9	56.9	56.9	56.9	56.9
857	59.5	59.5	51.4	59.5	59.5	59.5
866	59.8	61	57.2	58.6 	58.6	59.8
873	55.7	52.8	51.4	56.8	52.8	--
888	52.8	52.8	--	--	--	--
891	52.8	52.8	--	--	--	--

Note. T, Hylotelephium spectabile; C, Sedum sarmentosum; H, Pinus koraiensis; S, Pinus sylvestris var. mongolica; Y, Populous tomentosa; L, Salix babylonica. Dotted line means the primers didn’t fit the species.

Table S2 The primers we have selected and the annealing temperature (℃) for RAPD
primers	T	C	H	S	Y	L
01	37	37	36	36	--	36
02	37	36	36	36	36	36
03	37	--	--	--	36	36
04	37	37	36	36	36	36
05	--	37	--	--	36	36
06	37	37	36	36	36	36
07	--	37	36	36	36	36
08	37	--	36	36	--	--
09	--	37	36	36	36	37
10	37	37	36	36	36	--
11	37	37	36	--	36	--
12	37	37	37	36	--	36
13	--	--	--	--	37	36
14	37	--	--	36	--	--

Note. T, Hylotelephium spectabile; C, Sedum sarmentosum; H, Pinus koraiensis; S, Pinus sylvestris var. mongolica; Y, Populous tomentosa; L, Salix babylonica. Dotted line means the primers didn’t fit the species.



Table S3 The sequences of selected primers for ISSR. 
primers	Sequences (from 5’ to 3’ )
807	AGAGAGAGAGAGAGAGT
811	GAGAGAGAGAGAGAGAC
826	ACACACACACACACACC
827	ACACACACACACACACG
836	AGAGAGAGAGAGAGAGYA
842	GAGAGAGAGAGAGAGAYG
851	GTGTGTGTGTGTGTGTYG
856	ACACACACACACACACYA
857	ACACACACACACACACYG
866	CTCCTCCTCCTCCTCCTC
873	GACAGACAGACAGACA
888	BDBCACACACACACACA
891	HVHTGTGTGTGTGTGTG

Note.  Y = (C , T) ,B = (C , G, T),D = (A , G, T), H = (A , C , T),V = (A , C , G)


primers	Sequences (from 5’ to 3’ )
01	CGTTGGCCG
02	GACTGCCTCT
03	ATCGGCTTGGG
04	CTTGCCCACG
05	GTCGTTCCTG
06	TGAGGGTCCC
07	CCGCGTCTTG
08	GGCGAAGGTT
09	CATCCCCCTG
10	GGGTAACGCC
11	GGACTGGAGT
12	TCGGGGATAG
13	AGCCAGCGAA
14	TTCCGAACCC

Table S4 The sequences of selected primers for RAPD.




Fig S1 Part of the ISSR and RAPD bands detected by electrophoresis agarose gel.
M, size standards (100bp Ladder Ⅲ DingGuo, China)



Effects of different preservation methods on inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) molecular markers in botanic samples

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What is an endocrine disruptor?

Structural and functional evidences for the interactions between nuclear hormone receptors and endocrine disruptors at low doses

Wing geometry of <i>Phlebotomus stantoni</i> and <i>Sergentomyia hodgsoni</i> from different geographical locations in Thailand

CV2 - Editorial Board/Français

Sommaire / Contents

Effet de plusieurs méthodes de scarification et du stress osmotique sur la germination des graines de <i>Robinia pseudoacacia</i> L.

Gene expression profile of estrogen receptors alpha and beta in rat brain during aging and following high fat diet

Impact of greenspaces in city on avian species richness and abundance in Northern Africa

A new species of <i>Discocyrtanus</i> from Mato Grosso, Brazil (Opiliones: Gonyleptidae) with a key to the species of the genus

Effects of different preservation methods on inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) molecular markers in botanic samples

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