Multidisciplinary Digital Publishing Institute (Switzerland)
Multidisciplinary Digital Publishing InstituteNot a member yet
1861300 research outputs found
Sort by
A Study on the Development and Applicability of a Landscape Planning Model Platform
No full textThis study aims to establish an integrated landscape planning model and explore its applicability through the convergence of digital twin technology. The primary goal is to address the fragmented implementation of landscape policies and to provide a systematic framework that enhances efficiency and visualization in the planning process. To this end, text-mining analysis was conducted to extract relevant laws, statutory plans, and project data, thereby identifying key factors for model construction. The resulting model integrates conservation-oriented and recreation-oriented modules, presenting a practical approach for landscape management. Furthermore, by utilizing Blender 3D and OpenStreetMap, this study demonstrates the process through which a digital twin visualizes and simulates the spatial characteristics of the actual target site, thereby validating its utility in decision-making and stakeholder communication. The results indicate that the landscape planning model was reconfigured and integrated into 6 detailed implementation measures and 41 specific indicators. Moreover, the model visually linked 36 laws and approximately 70 plans and projects. Ultimately, the study confirms that the proposed approach provides a dynamic, data-driven platform for sustainable landscape management
Adherence to the Mediterranean Diet and Metabolic Gene Expression in Smokers: An Integrative Transcriptomic Approach
No full textBackground: Cigarette smoking disrupts cellular energy metabolism and remains a major global health problem. The Mediterranean diet, characterized by antioxidant and anti-inflammatory properties, has been implicated in the regulation of metabolic pathways. Objective: This study aimed to examine the association between adherence to the Mediterranean diet and the expression of energy metabolism-related genes in smokers aged 18–55 years. Methods: Smokers were classified according to their Mediterranean Diet Adherence Screener (MEDAS) scores into an adhering group (n = 24) and a non-adhering group (n = 24). Participant characteristics were recorded, blood samples were collected, and total RNA was isolated. Gene expression analysis was performed using a custom RT-qPCR array targeting energy metabolism-related genes. Pathway enrichment analysis was conducted using EnrichR Reactome 2024, and gene–metabolite relationships were explored using MetaboAnalyst 6.0 to support pathway-level interpretation. Results: Smoking was associated with coordinated upregulation of genes involved in glycolysis, glucose transport, lipid metabolism, amino acid metabolism, the pentose phosphate pathway, and redox regulation, consistent with a metabolically stressed state. In contrast, adherence to the Mediterranean diet was associated with lower expression of genes related to glycolytic flux, lipid β-oxidation, and amino acid turnover, alongside relatively higher engagement of tricarboxylic acid cycle-related pathways and reduced activation of redox-associated processes. Conclusions: Adherence to the Mediterranean diet was associated with differences in the expression of genes involved in cellular energy metabolism among smokers, suggesting a potential modulatory role of dietary patterns in smoking-related metabolic alterations
Methodology for Selecting an Electric Pump and Battery Pack for a Low-Thrust LRE: Computational Simulation Study
No full textThe paper offers a practical method for selecting an electric pump and battery pack for low-thrust liquid rocket engines. The approach combines 0D/1D modeling of hydraulic, electromechanical, and thermal subsystems in a single environment and is supplemented by sensitivity analysis, correlation analysis, and Monte Carlo simulation with N = 3000 iterations to verify the stability of the estimates. The methodology has been tested on mission profiles in the 5–50 kN thrust range and shows that the electric pump scheme is most effective at low thrusts, while an increase in thrust leads to a disproportionate increase in energy and thermal loads and narrows the scope of applicability. The determining factors remain the hydraulic efficiency of the pump η_pump and the oxidizer pressure; electrical parameters such as bus voltage and internal battery resistance have less influence and become noticeable at high power levels. Modeling confirms the stability of the obtained estimates; at a thrust of 20 kN, the spread of the battery block mass is close to ±10%. The proposed methodology provides quantitative guidelines in the early stages of design and helps to justify the scope of application of electric pump liquid rocket engines; expansion beyond low thrust requires progress in battery technologies
Botulism in a Dog Fed a Raw Meat-Based Diet: A Case Report
No full textRaw meat-based diets (RMBDs) have become increasingly popular among pet owners, despite well-documented risks of contamination with pathogenic bacteria capable of causing severe illness in companion animals. This report describes a fatal case of botulism in a 3-year-old female Labrador Retriever weighing 37 kg that was fed exclusively RMBD. The dog presented with acute-onset flaccid paralysis of the limbs approximately 48 h after possible ingestion of decomposing raw meat discarded in household waste. Supportive therapy, including fluid administration, nutritional support and eventual mechanical ventilation was provided. However, the patient developed progressive respiratory failure and died. The presence of Clostridium botulinum type C neurotoxin was confirmed in the dog serum by neutralization test in mice. The case suggests RMBD as a potential source of botulism toxins, particularly when derived from improperly stored meat products. The findings underscore the importance of detailed dietary history in dogs presenting with acute flaccid paralysis and reinforce the need for heightened awareness regarding the microbiological risks associated with raw feeding practices
Impact of Subway Platform Screen Door Opening and Closing on Particulate Matter Concentration Distribution at Different Locations and Times: The Case of Xi’an
No full textTo explore how the opening and closing of subway platform screen doors (PSD) affect particulate matter concentrations (PM10, PM2.5, and PM1.0) across different times (morning peak, off-peak, evening peak), and three key locations (subway tunnels, platforms, and waiting areas), we studied the Xi’an subway using a systematic monitoring approach, and a total of 6 monitoring points were monitored at 3 locations for 60 consecutive days of testing. The sampling time for each measurement point was 20 min, and a total of three groups were tested. The relationship between the opening/closing status of PSD and changes in particulate matter concentrations was then analyzed using statistical methods. The results showed that the particulate matter concentrations followed a sequential pattern: tunnel concentrations were higher than those in the waiting area, which in turn were higher than those at the platform center. PM10 concentrations exceeded China’s standards for indoor air quality (GB/T 18883-2022) at all three locations. For PM2.5, concentrations in the tunnel and waiting area exceeded the standard, while those at the platform center remained within the limit. Particles smaller than 1.0 μm constituted the dominant fraction of particulate matter in the tunnel, waiting area, and platform center. After the PSD opened, the peak average concentrations of PM10, PM2.5, and PM1.0 in the waiting area increased by 70.53%, 55.81%, and 42.41%, respectively, compared to the average concentrations before the train entered the station. PSD had a significant impact on fine particulate matter concentrations on the platform during the evening peak: PM10 concentrations in the front and rear of the waiting area were 37.85% and 57.61% higher than those in the middle, while PM2.5 concentrations in these two areas were 39.81% and 50.23% higher than in the middle. No obvious distribution pattern was observed for PM1.0. These results provide reference data for optimizing indoor air quality in the Xi’an subway and regulating the operation of platform screen doors
Serological Insights into Infectious Agents Circulating in Lithuanian Goats
No full textPathogens such as Toxoplasma gondii, lentiviruses (e.g., CAE), Hypoderma spp., Neospora caninum, Mycoplasma spp., and pestiviruses are important for goat farming in Lithuania; however, data on their prevalence remain limited. To address this gap, a multi-pathogen study was conducted between 2021 and 2024 using selected ELISA kits (ID.vet, Innovative Diagnostics, France). A total of 380 blood samples were collected from 30 goat herds across different regions of Lithuania; the sample size varied depending on the pathogen. Serum samples were tested for antibodies, and seroprevalence was calculated for each pathogen. The highest seroprevalence was detected for T. gondii (38.9%, 143/368) and CAE virus (19.5%, 74/380). Antibodies to Mycoplasma spp. (0.3%, 1/368), Hypoderma spp. (3.8%, 7/184), and N. caninum (0.5%, 2/368) were detected only sporadically, while no antibodies to Border disease virus or Q fever were identified. Mixed infections were found in 7.6% of samples. Chi-square analysis showed that co-infections with toxoplasmosis and CAE occurred more frequently than expected (χ2 = 19.05, p < 0.001). Herd size was significantly associated only with CAE seroprevalence (χ2 = 7.913, df = 1, p < 0.05). Overall, toxoplasmosis and CAE were identified as the most epidemiologically relevant infections in the Lithuanian goat population
Effects of Irrigation Practices and N Addition Rates on Wheat Nutrient Accumulation and Utilization in Dryland
No full textIrrigation practices and nitrogen (N) addition play pivotal roles in wheat production, and their rational coordination can significantly enhance N, phosphorus (P), and potassium (K) use efficiency and yield of wheat. However, the comprehensive effects of irrigation practices and N addition rates on N, P, and K accumulation and utilization and yield of wheat in dryland remain unclear. A field experiment with two irrigation practices (W0, zero-irrigation and W1, one-off irrigation), and four N addition rates (0, 120, 180, and 240 kg N ha−1, represented by N0, N120, N180, and N240, respectively) was conducted in 2021–2022 and 2023–2024. Compared to W0N0, W1N180 significantly increased wheat grain yield, spike number, and grains per spike by 46.4%, 35.9%, and 18.9%, respectively. Wheat yield and N, P, and K accumulation reached the maximum value at N180 or N240. One-off irrigation significantly improved the uptake efficiency and fertilizer partial factor productivity for N, P, and K, whereas increased N addition enhanced these parameters specifically for P and K. However, N180 treatment increased N uptake efficiency, N fertilizer partial factor productivity, P internal efficiency, and K internal efficiency by 22.2%, 31.1%, 9.4%, and 5.9%, respectively, compared to N240 under one-off irrigation. In addition, W1N180 significantly increased above-ground N, P, and K accumulation by 45.8%, 52.8%, and 51.8%, as well as pre-anthesis N and P translocation by 48.5% and 47.0%, respectively, compared to W0N120. Consequently, the W1N180 strategy not only improved wheat yield but also optimized N, P, and K accumulation, pre-anthesis N and P translocation, and nutrient use efficiency. Therefore, one-off irrigation combined with N180 can be recommended for enhancing wheat yield and nutrient use efficiency in dryland
Evaluating the Effects of Full-Fat Yogurt Consumption on Circulating Inflammatory Biomarkers and Ex Vivo Peripheral Blood Mononuclear Cell Inflammatory Responses in a Randomized-Controlled Crossover Trial
No full textChronic, low-grade inflammation is a characteristic of metabolic diseases like type 2 diabetes. Despite recommendations to select low- or non-fat dairy foods over full-fat dairy foods for metabolic health, recent research suggests potential anti-inflammatory benefits of dairy fat consumption. We aimed to compare the systemic inflammatory tone (i.e., circulating inflammatory biomarker concentrations and ex vivo peripheral blood mononuclear cell inflammatory responses) of individuals with prediabetes after consuming diets with full-fat (3.25%) or non-fat yogurt. We hypothesized that short-term consumption of three daily full-fat yogurt servings beneficially affects inflammatory tone. Thirteen participants aged 45–75 years completed an eight-week randomized, double-masked, controlled crossover study. The two, three-week experimental diets comprised three daily servings of full-fat or non-fat yogurt and were each preceded by a one-week run-in diet. Following each diet, circulating inflammatory biomarkers and cytokine concentrations in the supernatants of peripheral blood mononuclear cells under control or lipopolysaccharide-stimulated conditions were measured. Compared with non-fat yogurt intake, circulating immature granulocyte concentrations were lower following full-fat yogurt intake, but there were no other differences in leukocyte concentrations. Circulating concentrations of cytokines or other inflammatory markers did not differ by diet. Cell supernatant interleukin-1β concentrations were lower following the full-fat yogurt diet under unstimulated conditions but were not different between diets under stimulated conditions. There were no differences by diet in supernatant concentrations of other cytokines under unstimulated or stimulated conditions. Together, minimal differences in inflammatory tone were observed following the short-term consumption of three daily servings of full-fat or non-fat yogurt in individuals with prediabetes
Novel Hit Compounds Against a Neglected Sexually Transmitted Infection: Synthesis and Trichomonacidal Activity of 1,3-Thiazolidin-4-One Derivatives
No full textBackground: Infections caused by the protozoan Trichomonas vaginalis affect millions of people worldwide and are responsible for one of the most common sexually transmitted diseases. Despite the efficacy of 5-nitroimidazoles like metronidazole, concerns regarding widespread resistance and the absence of viable alternatives for specific patient populations necessitate the development of structurally diverse pharmacological agents. In this study, we investigated the antiparasitic activity of 1,3-thiazolidin-4-one derivatives against T. vaginalis. Methods: Thiazolidines were synthesized via multicomponent reaction (MCR) using one-pot methodology and tested in vitro against the parasite and mammalian cell lines. Results: Seventy percent of the compounds showed more than 80% antiparasitic activity at 100 μM, with compounds 4a, 4b, and 4f exhibiting IC50 ≤ 20 µM. None of the molecules exhibited cytotoxic against Vero CCL-81 and HeLa cells. Evaluation of the structure–activity relationship (SAR) indicates that the substituent at the nitrogen position of the heterocycle may be involved in the antiparasitic effect of these compounds. In silico studies also revealed that the three compounds possess adequate oral bioavailability and do not present mutagenic, tumorigenic or irritating risks. Finally, molecular docking predicted strong interactions of compounds 4a, 4b, and 4f with T. vaginalis enzymes lactate dehydrogenase and purine nucleoside phosphorylase; compound 4f also interacted with methionine Ƴ-lyase. Conclusions: These preliminary results suggest that 1,3-thiazolidin-4-ones are promising scaffolds for developing new trichomonacidal agents
Antimicrobial and Antibiofilm Activity of a Lactobacillus reuteri SGL01, Vitamin C and Acerola Probiotic Formulation Against Streptococcus mutans DSM20523
No full textDental caries is a multifactorial chronic infectious disease that impacts healthcare costs globally, caused by alterations of the plaque microbiome and proliferation of cariogenic Streptococcus mutans. Treatments targeting S. mutans, such as alternative strategies using probiotics, might be effective in preventing the development of dental caries. In this study, the probiotic formulation of Lactobacillus reuteri SGL01, vitamin C, and acerola was tested against S. mutans DSM20523. Antimicrobial activity was assessed by deferred antagonism and spot-on-lawn assays for L. reuteri SGL01. MIC and MBC of L. reuteri SGL01 cell-free supernatant (CFS), vitamin C, and acerola were determined with the microdilution method. Time–kill assays determined the bactericidal kinetics for each compound. The checkerboard method was used to evaluate the potential synergistic activity of CFS–vitamin C or CFS–acerola at scalar dilutions from 1 to 8X MIC. Lastly, antibiofilm activity was tested for each compound. Antimicrobial activity of L. reuteri SGL01 was first assessed by classic methods. MIC and MBC values differed for one dilution for all compounds, with values of 25% and 50% for CFS, 9.3 mg/mL and 18.7 mg/mL for vitamin C, and 18.7 mg/mL and 37.5 mg/mL for acerola, respectively. Moreover, time–kill assays confirmed the bactericidal activity at different timepoints: 4 h for CFS, 6 h for vitamin C, and 24 h for acerola. The fractional inhibitory concentration index (FICI) showed indifference for all combinations, and for associations tested at 2, 4, and 8XMIC. S. mutans biofilm production was impaired for all components, with stronger activity by vitamin C and acerola at lower concentrations. The probiotic formulation containing L. reuteri SGl01, vitamin C, and acerola extract exerts a bactericidal effect, especially strong for the CFS, as well as antibiofilm activity. Thus, the combination of these three components could be advantageous for their complementary effects, with use as a novel treatment against the development of dental caries by S. mutans