Multidisciplinary Digital Publishing Institute (Switzerland)
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Smartphone Photogrammetry as a Tool for Pes Planus Assessment: Reliability and Agreement with Radiographic Measurements
No full textBackground/Objectives: The purpose of this study was to evaluate the reliability and diagnostic accuracy of smartphone-based photogrammetry for the assessment of pes planus and to determine its agreement with standard radiographic measurements. Methods: This prospective diagnostic study included 100 skeletally mature patients (50 males, 50 females; mean age 43.4 years) who underwent standardized lateral weight-bearing foot radiographs and smartphone-based foot photography. The calcaneal pitch angle (CPA) was measured on radiographs, and a corresponding photographic arch pitch angle (P-APA) was measured from standardized smartphone photographs using digital software (Angle Meter iOS v1.9.8). Three independent observers performed each measurement twice. Inter- and intra-observer reliability was assessed using intraclass correlation coefficients (ICC). Agreement between methods was evaluated with Pearson correlation, Lin’s concordance correlation coefficient (CCC), Bland–Altman analysis, and Deming regression. Receiver operating characteristic (ROC) analysis was performed to determine the diagnostic accuracy of calibrated P-APA, with the radiographic threshold of 18° serving as the reference standard for pes planus classification. Results: All measurements demonstrated excellent intra- and inter-observer reliability (ICC ≥ 0.900). P-APA values were systematically higher than radiographic values (31.8° ± 4.3 vs. 21.8° ± 5.5; p < 0.001). A strong correlation was observed between the two methods (r = 0.799, p < 0.001), but concordance was poor (CCC = 0.222). Bland–Altman analysis revealed a mean bias of +10.1° with wide limits of agreement (3.8° to 16.4°). Deming regression yielded the calibration equation Radiographic CPA = (P-APA × 1.371) − 21.883. ROC analysis of calibrated values yielded an AUC of 0.885 (95% CI, 0.820–0.951), with an optimal cutoff of 22.8° (sensitivity, 100%; specificity, 61.1%), corresponding to 32.6° on the uncalibrated photographic scale. Conclusions: Conventional weight-bearing radiography remains the reference standard for diagnosis and clinical decision-making in pes planus. The smartphone-derived photographic arch pitch angle is a non-equivalent surrogate measure that shows substantial systematic bias and limited agreement with radiographic calcaneal pitch, and therefore cannot replace weight-bearing radiographs. Smartphone photogrammetry may be used only as a complementary tool for preliminary screening or telemedicine support; any positive or equivocal findings require radiographic confirmation
Secondary Education Teachers and Climate Change Education: A Complementary Bibliometric and Methodological Review
No full textClimate change is the most significant socio-environmental challenges of our time, and education has been recognized as a fundamental strategy to confront it. Yet research efforts have focused more on students than on teachers, despite the latter’s key role in mediating between scientific and curricular knowledge and classroom practice. This study set out to characterize the field of educational research on climate change from the perspective of secondary school teachers. To this end, we conducted a systematic review and bibliometric analysis of 50 peer-reviewed studies from 15 countries (2010–2023). The results show a growing interest over time, with increases associated with international milestones such as the IPCC reports and the Paris Agreement, while declines are observed in connection with political shifts and the COVID-19 pandemic. Consolidated academic reference points were identified, including Eric Plutzer and Maria Ojala, alongside influential international organizations such as the IPCC and UNESCO, suggesting the presence of schools of thought and institutional frameworks that structure the field. Methodologically, descriptive and exploratory studies predominate, with a notable reliance on qualitative and mixed-methods designs using small samples, reinforcing the difficulty of accessing teachers as a research population. Overall, this review highlights significant gaps, particularly the geographical bias toward the Global North, and underscores the urgency of broader, more inclusive, and critically engaged research that positions teachers as essential agents of transformative educational responses to the climate crisis
Study on Dual-Targeted Liposomes Containing Curcumin-Copper Chelate in the Treatment of Triple-Negative Breast Cancer
No full textBackground: Triple-negative breast cancer (TNBC) remains primarily treated with chemotherapy due to the lack of effective therapeutic targets, but this approach carries significant systemic toxicity and a high risk of drug resistance. Curcumin (Cur), despite its multifaceted antitumor activity, faces limitations in clinical application due to poor water solubility and weak targeting properties. This study aims to develop a folate/mitochondria dual-targeted curcumin–copper chelate liposome (Cu-Cur DTLPs) formulation that enables copper accumulation within tumor cells and induces copper-mediated cell death, thereby providing an effective and relatively low-toxicity therapeutic strategy for triple-negative breast cancer. Methods: Curcumin–copper chelates (Cu-Cur) were first synthesized and characterized using mass spectrometry, NMR, and infrared spectroscopy. Subsequently, dual-targeted liposomes (Cu-Cur DTLPs) were prepared via the thin-film dispersion method, with systematic evaluation of particle size, zeta potential, encapsulation efficiency, and in vitro release profiles. In vitro cytotoxicity was assessed against 4T-1 and MDA-MB-231 cells using the MTT assay. In a 4T-1 tumor-bearing BALB/c mouse model, comprehensive evaluation of targeting efficiency, antitumor efficacy, and mechanisms of action was conducted via in vivo imaging, tumor volume monitoring, immunohistochemistry (detecting FDX1 and DLAT proteins), and TUNEL staining. Results: Cu-Cur DTLPs with a uniform particle size of approximately 104.4 nm were successfully synthesized. In vitro and in vivo studies demonstrated that compared to free curcumin and conventional liposomes, Cu-Cur DTLPs significantly enhanced drug accumulation in tumor tissues and exhibited effective tumor growth inhibition. Mechanistic studies confirmed that this formulation specifically accumulates copper ions within tumor cells, upregulates FDX1, promotes DLAT oligomerization, and induces mitochondrial dysfunction, thereby driving copper death. TUNEL staining ruled out apoptosis as the primary mechanism. Safety evaluation revealed no significant toxicity in major organs. Conclusions: The Cu-Cur DTLPs developed in this study effectively induce copper-mediated death in TNBC through a dual-targeted delivery system, significantly enhancing antitumor activity with favorable safety profiles. This establishes a highly promising novel nanotherapeutic strategy for TNBC treatment
Genetic Basis of Familial Cancer Risk: A Narrative Review
No full textFamilial cancers are caused by inherited mutations in specific genes that regulate cell growth, division, and repair. Approximately 5–10% of all cancer cases have a hereditary component, where germline mutations in certain genes increase an individual’s susceptibility to developing cancer. Two major categories of genes are involved in cancer development: tumour suppressor genes and oncogenes. Both play critical roles in regulating normal cell behaviour, and when mutated, they can contribute to uncontrolled cell proliferation and tumour formation. In addition to genetic mutations, epigenetic alterations also play a significant role in familial cancer. Epigenetics refers to changes in gene expression due to DNA methylation, histone modifications, and the dysregulation of non-coding RNAs without alter the underlying DNA sequence. Familial cancer syndromes follow various inheritance patterns, including autosomal dominant, autosomal recessive, X-linked, and mitochondrial inheritance, each with distinct characteristics. Identifying genetic mutations associated with familial cancers is a cornerstone of genetic counselling, which helps individuals and families navigate the complex intersection of genetics, cancer risk, and prevention. Early identification of mutations enables personalized strategies for risk reduction, early detection, and, when applicable, targeted treatment options, ultimately improving patient outcomes
Scenario-Based Land-Use Trajectories and Habitat Quality in the Yarkant River Basin: A Coupled PLUS–InVEST Assessment
No full textLand use/cover change (LUCC) is a dominant driver of ecosystem service dynamics in arid inland basins. Focusing on the Yarkant River Basin (YRB), Xinjiang, we coupled the PLUS land-use simulation with the InVEST Habitat Quality Model to project 2040 land-use patterns under four policy scenarios—Natural Development (ND), Arable Protection (AP), Ecological Protection (EP), and Economic Development (ED)—and to quantify their impact on habitat quality. Model validation against the 2020 map indicated strong agreement (Kappa = 0.792; FOM = 0.342), supporting scenario inference. From 1990 to 2023, arable land expanded by 58.17% and construction land by 121.64%, while forest land declined by 37.45%; these shifts corresponded to a basin-wide decline and increasing spatial heterogeneity of habitat quality. Scenario comparisons showed the EP pathway performed best, with 32.11% of the basin classified as very high-quality habitat and only 8.36% as very low-quality. In contrast, under ED, the combined share of very low + low quality reached 11.17%, alongside greater fragmentation. Spatially, high-quality habitat concentrates in forest and grassland zones of the middle–upper basin, whereas low-quality areas cluster along the oasis–desert transition and urban peripheries. Expansion of arable and construction land emerges as the primary driver of degradation. These results underscore the need to prioritize ecological-protection strategies especially improving habitat quality in oasis regions and strengthening landscape connectivity to support spatial planning and ecological security in dryland inland river basins
Associations Between Perceived Physical Literacy and DXA-Measured Body Composition in Spanish Adolescents: The ENERGYCO Study
No full textBackground: Physical literacy is a multidimensional construct that may be relevant for promoting active lifestyles and healthy development during adolescence. However, the association between perceived physical literacy (PPL) and body composition assessed by dual-energy X-ray absorptiometry (DXA) remains underexplored. Objective: To examine the association between PPL and DXA-derived body composition parameters in Spanish adolescents. Methods: This cross-sectional study included 56 adolescents (13.2 ± 1.27 years, 28.6% girls). PPL was assessed using the validated Spanish version of the Perceived Physical Literacy Instrument (S-PPLI). Body composition was measured by DXA. Associations between PPL and body composition outcomes were examined using general linear models, adjusting for sex, age, and device-measured moderate-to-vigorous physical activity (MVPA) and sedentary time. Results: Higher PPL was significantly associated with greater lean body mass (β = 0.81; p = 0.02), lean mass index (β = 0.22; p = 0.01), and fat-free mass (β = 0.85; p = 0.01), as well as with higher body mass index (BMI) (β = 0.24; p = 0.03). Conclusions: Higher PPL is associated with more favorable lean-related body composition outcomes in Spanish adolescents, whereas no associations were found with adiposity or bone parameters. These findings highlight PPL as a relevant correlation of lean body composition during adolescence. Given the cross-sectional design, causal inferences cannot be drawn, and future longitudinal and interventional studies are warranted
The Impact of Digitization Transport Documents on the Competitiveness of Road Freight Transport Companies
No full textBackground: The rapid digital transformation in logistics requires the adaptation of transport companies to electronic information management, particularly through the implementation of electronic consignment notes (e-CMR). This study examines how the digitization of transport documentation affects the competitiveness, operational efficiency, and environmental performance of road freight transport companies. Methods: A questionnaire survey was conducted among Slovak and Czech carriers to analyze their experience and readiness for adopting e-CMR. The collected data were evaluated using descriptive and comparative methods to quantify economic and ecological impacts, focusing mainly on invoicing efficiency and paper consumption. Results: The results show that only a small share of carriers currently use e-CMR, primarily due to high software costs and the lack of partner participation. Nevertheless, digitization can significantly shorten the average invoicing delay by approximately 11.5 days, releasing around 7% of tied-up working capital and improving cash flow. From an environmental perspective, the replacement of paper CMR forms could save millions of sheets annually, leading to a substantial reduction in CO2 emissions and paper waste within the V4 region. Conclusions: The findings confirm that the adoption of e-CMR improves economic performance, increases transparency, and contributes to sustainability, representing a crucial step toward a more competitive and environmentally responsible road freight transport sector in Europe
Influence of Posture, Spinal Level, Gender and Muscle Activation on Biomechanical Properties of Lumbar Erector Spinae in Healthy Young Adults
No full textBackground and Objectives: This study set out to better understand how posture, spinal level, gender and muscle activation influence the biomechanical properties of the lumbar erector spinae (LES) in healthy young adults. We aimed to measure how these factors influence LES tone, stiffness, and damping using a myotonometry device. Materials and Methods: Thirty healthy young adults (14 males, 16 females; aged 20–25 years) were evaluated at bilateral L3–L5 levels in prone, unsupported sitting, and standing positions, both under relaxed conditions and during submaximal isometric lumbar extension. The myotonometer measured LES tone (Hz), stiffness (N/m), and damping (logarithmic decrement). For each outcome, a mixed-model repeated-measures ANOVA was conducted with Gender as a between-subject factor and Posture, Level, and Action (relaxed vs. contracted) as within-subject factors (Bonferroni-adjusted α = 0.0167). Results: Posture produced the most significant and consistent effects on all properties—stiffness, tone, and damping (p < 0.0167)—with sitting and standing generally increasing stiffness and tone compared to prone, and sitting showing the highest values. Gender significantly impacted stiffness and tone (p < 0.0167), with males showing higher values. Spinal level also significantly influenced damping, stiffness, and tone (all p < 0.0167), with differences more apparent in females. Significant interactions included the influence of Posture × Gender on tone and damping (p < 0.0167), and of Posture × Action on stiffness and tone (p < 0.0167), alongside a strong three-way interaction for Level × Action × Posture across all outcomes, suggesting posture-related responses depend on activation state and spinal level. Conclusions: LES biomechanical properties are strongly affected by posture and further modulated by muscle activation, gender, and spinal level. These results support the creation of posture- and gender-specific reference values and underscore the value of dynamic, posture-specific myotonometer-based assessments for paraspinal muscle evaluation and clinical planning
Endoperoxides: Highly Oxygenated Terpenoids with Anticancer and Antiprotozoal Activities
No full textEndoperoxides constitute a distinctive class of highly oxygenated terpenoids defined by the presence of a cyclic peroxide (–O–O–) bond, a structural motif responsible for their pronounced chemical reactivity and diverse biological effects. Naturally occurring endoperoxide-containing terpenoids are broadly distributed across terrestrial and marine taxa, including higher plants, algae, fungi, and bryophytes, where they are believed to participate in chemical defense and ecological interactions. This review provides a comprehensive overview of naturally occurring endoperoxide terpenoids, focusing on their natural sources, structural diversity, and reported biological activities. Particular emphasis is placed on compounds exhibiting antiprotozoal and antitumor activities, exemplified by artemisinin and its derivatives, which remain cornerstone agents in antimalarial therapy and continue to attract interest for their anticancer potential. Structure–activity relationship (SAR) analysis, supported by computational prediction using the PASS (Prediction of Activity Spectra for Substances) platform, is employed to examine correlations between peroxide-containing frameworks and biological function. Comparative assessment of experimental data and predicted activity profiles identifies key structural features associated with antiprotozoal, antineoplastic, and anti-inflammatory effects. Collectively, this review highlights endoperoxides as a valuable and chemically distinctive class of bioactive natural products and discusses their promise and limitations as leads for further pharmacological development, particularly in light of their intrinsic reactivity and stability challenges
Investigation of a PID-Based Dynamic Illuminance Control System for Intelligent Neonatal Jaundice Phototherapy Using a Blue Light LED Array
No full textNewborns are unable to reliably express changes in their physical condition due to their physiological immaturity and limited capacity for communication; therefore, continuous and systematic monitoring during phototherapy is essential to ensure timely detection of adverse responses and maintenance of therapeutic safety. This study extends our prior work, which introduced an indirect method for measuring light intensity to improve precision in monitoring newborn skin illumination. Light-emitting diode (LED) phototherapy has attracted considerable attention as an effective treatment for neonatal jaundice (NNJ). This study introduces an three-dimensional configuration of blue LEDs. An Arduino Mega 2560 microcontroller with pulse-width modulation (PWM) technology was employed to independently regulate the intensity of LED strips, enabling precise control of light output. The strips were mounted on an arc-shaped structure that can be adjusted mechanically and electronically through pre-programmed instructions embedded in the microcontroller. The results demonstrate that blue light at a wavelength of 460 ± 10 nm aligns with the peak absorption spectrum of bilirubin, thereby optimizing the efficacy of phototherapy for NNJ. Both observed absorption peaks were within the therapeutically effective range. Computer simulations confirmed that stable output contours can be achieved using rapid electronic scanning with a PID control algorithm to dynamically adjust the duty cycle. Experimental data showed that LED radiation output was largely linear. This supports the use of linear control algorithms and confirms the platform’s feasibility for future research