Multidisciplinary Digital Publishing Institute (Switzerland)
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Application of Graphene Oxide Nanomaterials in Crop Plants and Forest Plants
No full textGraphene oxide (GO) is a carbon-based nanomaterial explored for agricultural and forestry uses, but plant responses are strongly subject to both the dose and the route of exposure. We summarized recent studies with defined graphene oxide (GO) exposures by seed priming, foliar delivery, and root or soil exposure, while comparing annual crops with woody forest plants. Mechanistic progress points to a shared physicochemical basis: surface oxygen groups and sheet geometry reshape water and ion microenvironments at the soil–seed and soil–rhizosphere interfaces, and many reported shifts in antioxidant enzymes and hormone pathways likely represent downstream stress responses. In crops, low-to-moderate doses most consistently improve germination, root architecture, and tolerance to salinity or drought stress, whereas high doses or prolonged root exposure can cause root surface coating, oxidative injury, and photosynthetic inhibition. In forest plants, evidence remains limited and often relies on seedlings or tissue culture. For forest plants with long life cycles, processes such as soil persistence, aging, and multi-seasonal carry-over become key factors, especially in nurseries and restoration substrates. The available data indicate predominant root retention with generally limited root-to-shoot translocation, so residues in edible and medicinal organs remain insufficiently quantified under realistic-use patterns. This review provides a scenario-based framework for crop- and forestry-specific safe-dose windows and proposes standardized endpoints for long-term fate and ecological risk assessment
Albumin Nanoparticles Harness Activated Neutrophils to Cross Vascular Barriers for Targeted Subcutaneous and Orthotopic Colon Cancer Therapy
No full textColorectal cancer (CRC) therapy faces challenges due to limited drug penetration across the blood–tumor barrier. Neutrophils, with their natural ability to migrate to inflamed and tumor sites, offer a promising cell-mediated delivery strategy. This study developed albumin nanoparticles loaded with 6-shogaol (NPs/6-shogaol) and utilized activated neutrophils as carriers to transport the nanoparticles across vascular barriers for colon cancer therapy. The physicochemical properties, biocompatibility, and targeting efficiency of the NPs were evaluated in vitro and in vivo. The formulated NPs/6-shogaol exhibited favorable physicochemical properties, including a uniform nano-scale size (~150 nm), negative zeta potential, and high drug loading efficiency. In both subcutaneous and orthotopic colon cancer models, neutrophil-mediated delivery significantly enhanced tumor accumulation of 6-shogaol, inhibited tumor growth, and induced apoptosis by suppressing neutrophil elastase (NE) expression. Notably, no significant systemic toxicity was observed. This neutrophil-hitchhiking albumin nanoplatform provides a targeted and biocompatible strategy for effective colon cancer therapy
Cultivar-Specific Responses in Postharvest Strategies to Preserve Phytochemical Profile in Traditional Serbian Peppers (Capsicum annuum L.)
No full textTraditional Serbian pepper cultivars ‘Kurtovska ajvaruša’, ‘Grkinja babura’, and ‘Duga bela ljuta’ were stored under different conditions (10 °C, 4 °C, and 4 °C with pre-storage hot water dipping-HWD) for 21 days plus a 3-day shelf life. The main quality parameters measured included mineral content, total soluble solids (TSS), titratable acidity (TA),sugar content (glucose, fructose), organic (ascorbic and citric) acid content, and total phenolic content (TPC). Principal component analysis (PCA) revealed cultivar-specific responses to storage treatments. Cultivar specificity is a crucial determinant in defining optimal conditions for the preservation of phytochemical composition. The cultivar ‘Kurtovska ajvaruša’ showed the highest retention of phenolic compounds when stored at 4 °C following hot water treatment (HWD), whereas ‘Grkinja babura’ should be stored at 4 °C (without hot water treatment, as it provides no additional benefits) for up to 21 days, as this ensures balanced preservation of sugar and organic acid contents while maintaining high sensory quality of the fruit. ‘Duga bela ljuta’ exhibited superior ascorbic acid preservation at 10 °C, reaching 104.4 mg/100 g. Optimizing postharvest storage conditions is essential for maintaining the nutritional quality of traditional pepper cultivars intended for both fresh consumption and processing
Rating and Spatial Pattern Analysis of Human–Land Symbiosis Relationship from an Ecological Perspective: A Case Study of the “Five Poles” Urban Agglomeration in the Yellow River Basin
No full textThe “Anthropocene” has witnessed unprecedented challenges to the sustainability of human development. Resolving the contradiction between humans and land and achieving coordinated development has become a pressing issue across many disciplines in the era of ecological civilization. This study adopts an ecological perspective to investigate the symbiotic relationship between humans and land in the “Five Poles” urban agglomerations of the Yellow River Basin. In this framework, ecosystem service value and human well-being are employed to quantify “human” and “land,” respectively. The Lotka–Volterra model is then applied as a structural analogy to quantify the dynamic interactions within this symbiotic relationship, treating ecosystem service value and human well-being as two interdependent systems with feedback mechanisms. For the “Five Poles” urban agglomerations in the Yellow River Basin, the ecosystem service and human well-being pressures, along with the symbiosis indices for the period 2011–2020, were calculated and categorized. The results were first subjected to a visual analysis to describe the spatial patterns. Subsequently, spatial autocorrelation analysis was employed to quantitatively investigate the clustering and heterogeneity of these patterns, thereby systematically elucidating the spatial characteristics of human–land symbiosis in the Yellow River Basin. The findings indicate that: (1) the human–land relationship in the Yellow River Basin has evolved from partial interaction to mutualism, reflecting improved coordination within the regional human–land system. (2) The evaluation of this relationship improved between 2011–2015 and 2016–2020. (3) High-evaluation areas have shifted from east to west, exhibiting distinct agglomeration characteristics
Finding Influencers Based on Social Interaction and Graph Structure in Social Media
No full textWith the development of online social media, influencer detection methods on these platforms have become an important area of study. However, existing influencer detection methods often place significant emphasis on the number of followers, which can lead to a drawback in maintaining the influence of users who have not been very active recently. In this paper, we propose an influencer detection method that takes both social interactions and the graph structure of social media into account. By considering both social interactions and graph structure, the proposed method prevents influence scores of users who have not been recently active from remaining disproportionately high. To demonstrate the superiority of the proposed method, we conducted a performance comparison with existing methods
Inhibitory Effect of Trichoderma longibrachiatum on Growth of Fusarium Species and Accumulation of Fumonisins
No full textFusarium spp. cause devastating crop diseases and produce carcinogenic mycotoxins such as fumonisins, threatening global food safety and human health. In this study, Trichoderma longibrachiatum A25011, isolated from apples in Aksu, Xinjiang, exhibited significant antagonistic activity with mycelial growth inhibition rates of 54.52% against F. verticillioides 48.62% against F. proliferatum, and 58.22% against F. oxysporum in confrontation assays. Enzyme activity detection revealed high chitinase (583.21 U/mg protein) and moderate cellulase (43.92 U/mg protein) production, which may have the capacity to degrade fungal cell walls. High-Performance Liquid Chromatography–Mass Spectrometry (HPLC-MS/MS) analyses enabled the quantification of fungal hormones including gibberellin A3 (GA3, 2.44 mg/L), cytokinins (cis-zeatin riboside (CZR): 0.69 mg/L; trans-zeatin riboside (TZR): 0.004 mg/L; kinetin: 0.006 mg/L), and auxins (indole-3-acetic acid (IAA): 0.35 mg/L; abscisic acid: 0.06 mg/L). Application of a T. longibrachiatum A25011 spore suspension around the roots of peanut plants enhanced growth by 13.20% (height), 5.65% (stem and leaf biomass), and 39.13% (root biomass). Notably, A25011 reduced F. proliferatum-derived fumonisin accumulation in rice-based cultures by 93.58% (6 d) and 99.35% (10 d), suggesting biosynthetic suppression. The results demonstrated that T. longibrachiatum strain A25011 exhibited excellent biocontrol capability against Fusarium spp., proving its dual role in simultaneously suppressing fungal growth and fumonisin accumulation while promoting plant growth. T. longibrachiatum A25011 could be applied as a multifunctional biocontrol agent in sustainable agriculture in the future
Comparative Study on Multi-Objective Optimization Design Patterns for High-Rise Residences in Northwest China Based on Climate Differences
No full textAs China’s urbanization rate continues to rise, the scale of high-rise residences also grows, emerging as one of the main sources of building energy consumption and carbon emissions. It is therefore crucial to conduct energy-efficient design tailored to local climate and resource endowments during the schematic design phase. At the same time, consideration should also be given to its impact on economic efficiency and environmental comfort, so as to achieve synergistic optimization of energy, carbon emissions, and economic and environmental performance. This paper focuses on typical high-rise residences in three cities across China’s northwestern region, each with distinct climatic conditions and solar energy resources. The optimization objectives include building energy consumption intensity (BEI), useful daylight illuminance (UDI), life cycle carbon emissions (LCCO2), and life cycle cost (LCC). The optimization variables include 13 design parameters: building orientation, window–wall ratio, horizontal overhang sun visor length, bedroom width and depth, insulation layer thickness of the non-transparent building envelope, and window type. First, a parametric model of a high-rise residence was created on the Rhino–Grasshopper platform. Through LHS sample extraction, performance simulation, and calculation, a sample dataset was generated that included objective values and design parameter values. Secondly, an SVM prediction model was constructed based on the sample data, which was used as the fitness function of MOPSO to construct a multi-objective optimization model for high-rise residences in different cities. Through iterative operations, the Pareto optimal solution set was obtained, followed by an analysis of the optimization potential of objective performances and the sensitivity of design parameters across different cities. Furthermore, the TOPSIS multi-attribute decision-making method was adopted to screen optimal design patterns for high-rise residences that meet different requirements. After verifying the objective balance of the comprehensive optimal design patterns, the influence of climate differences on objective values and design parameter values was explored, and parametric models of the final design schemes were generated. The results indicate that differences in climatic conditions and solar energy resources can affect the optimal objective values and design variable settings for typical high-rise residences. This paper proposes a building optimization design framework that integrates parametric design, machine learning, and multi-objective optimization, and that explores the impact of climate differences on optimization results, providing a reference for determining design parameters for climate-adaptive high-rise residences
Accelerated Genomic Evolution and Divergence of Escherichia coli Under Phage Infection Stress: Emphasizing the Role of IS Elements in Changing Genetic Structure
No full textThe phage-resistant mutant (PRM) strains of Escherichia coli (E. coli) exhibited abundant genetic and phenotypic diversity. IS elements played a vital role in creating various genetic divergences and regulating gene functions under phage infection stress. Genetic variations of PRM strains derived from E. coli MG1655 and mutation frequencies of coevolved E. coli populations with phages were explored by high-throughput sequencing and resequencing. Infrequent-restriction-site PCR (IRS-PCR) and carbon utilization test revealed the genetic and phenotypic diversity of the PRM strains. Numerous and discrepant mutation sites (MSs) were observed in the PRM strains and the coevolved populations, and many MSs were related to the synthesis of flagella and LPS, which often serve as receptors in a phage invasion. The insertions of various IS elements in key gene locations were also frequently found in the PRM strains, which indicate for the first time that IS elements played a vital role in generating genetic divergence and regulating gene functions under phage infection stress. Resequencing revealed that the coevolved populations at three evolving stages had discrepant profiles of MSs, and nearly all detected MSs occurred in the coevolved populations, which led to coexisting phages that increased the mutation rates and expedited the occurrence of the defective MSs in E. coli populations. In summary, our results reveal that the widespread and abundant presence of phages may provide one important force driving bacterial genomic evolution and prompt bacterial genetic divergence via accelerated mutation and increased mutation rates in the E. coli genome
The Study of Digital Forensics in KSA: Education, and Prosecution Capabilities: A Needs-Based Analysis
No full textThis investigation provides a critical analysis of the digital forensics field within the Kingdom of Saudi Arabia (KSA), specifically focusing on its educational systems, and the effectiveness of prosecutorial efforts. Utilizing a mixed-methodology framework and extensive literature reviews, this study reveals pronounced deficiencies in digital forensics against increased cybercrime activities. Furthermore, it highlights a general lack of preparedness among digital forensics professionals in KSA and notes significant variations in forensic applications across different judicial and educational contexts. The research recommends creating a uniform national educational framework for digital forensics, improving professional training programs, and strategically enhancing forensic technologies. Through a thorough analysis of demographic trends, educational programs, and adherence to procedural standards, this study proposes targeted strategies to fortify the digital forensic infrastructure of KSA, aligning with the strategic imperatives of Vision 2030
Candida albicans Meningoencephalitis After Vestibular Schwannoma Surgery: An Autopsy-Confirmed Case Report
No full textBackground and Clinical Significance: Cerebral candidiasis (Candida albicans meningoencephalitis) is a rare but severe central nervous system (CNS) infection, usually associated with neurosurgical procedures or indwelling devices. Diagnosis is challenging due to frequent negativity of cerebrospinal fluid (CSF) cultures, and mortality remains high despite antifungal therapy. Case Presentation: We describe a 64-year-old woman who underwent retrosigmoid resection of a left vestibular schwannoma. The early postoperative course was complicated by fever, neurological deterioration, and hydrocephalus requiring external CSF drainage. Multiple lumbar punctures revealed inflammatory CSF profiles but persistently negative cultures. One month post-surgery, intraoperative samples from mastoid repair material grew Candida albicans, prompting antifungal therapy. Despite treatment, the patient experienced fluctuating neurological status and required multiple external ventricular drains. Three months after surgery, she clinically deteriorated and died. Autopsy showed diffuse meningeal thickening and purulent exudates at the brain base and posterior fossa. Histopathology confirmed chronic lympho-histiocytic meningitis with necrotizing foci containing Candida albicans. Conclusions: This case underscores the diagnostic and therapeutic challenges of post-neurosurgical Candida CNS infections. Repeatedly negative CSF cultures delayed diagnosis, emphasizing the value of ancillary tests such as β-d-glucan and molecular assays. Even with antifungal therapy, prognosis is poor. Autopsy remains essential for uncovering fatal healthcare-associated fungal infections and informing clinical vigilance and medico-legal assessment