Sokoine University of Agriculture

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    Nutrient intake of women of child bearing age from two Agro-climatic zones of Rural Areas in Tanzania

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    Tanzania Journal of Agricultural Sciences (2020) Vol. 19 No. 2, 92-106Nutrient deficiencies impose significant public health problems in many developing countries affecting especially women of child bearing age and children. A household cross-sectional survey involving a sample of 351 households was conducted in four villages of Morogoro and Dodoma regions, representing two different agro-climatic regions, to examine nutrient intake from food in rural women of childbearing age. The respondent was the mother/caregiver in the household. Dietary intake data were collected by using a quantitative 24 hour dietary recall questionnaire. Nutrient intake was analyzed using NutriSurvey program. Nutrients consumed was classified into energy, protein, fat, carbohydrate, vitamin A, vitamin B 1 , vitamin B 2 , vitamin B 6 , vitamin B 12 , folic acid, vitamin C, calcium, iron, and zinc. The mean daily intake of energy was 1976 Kcal (95% CI: 1886.1-2066.5) for Morogoro and 1651 Kcal (95% CI: 1553.0-1747.0) for Dodoma. Daily mean intake of protein was 61.2g (95% CI: 56.9-65.4) for Morogoro and 36.7g (95% CI: 32.3- 41.1) for Dodoma. Mean fat intake was 33.0g (95% CI: 30.1-35.9) for Morogoro and 23.9g (95% CI: 20.8-27.0) for Dodoma. Vitamin A (retinol equivalents) intake in Dodoma was higher than the recommended amounts at 823.4g (95% CI: 710.1-916.1) but lower than the recommended intake in Morogoro at 335.3g (95% CI: 271.0-400.0). Even though both districts did not meet the recommended nutrient intake for vitamin B12, Dodoma had the lowest intake at 0.6mg (95% CI: 0.31, 0.83) compared to Morogoro 1.5 mg (95% CI: 1.13, 1.84). The results of this study suggest different nutrient consumption patterns between Dodoma and Morogoro based on the agro- climatic condition of the districts which also influence the type of foods produced in particular areas. This calls for support for education on food and dietary intake that purposely accentuate increasing production and consumption of a diversity of foods preferred in a certain agro-climatic environment. This should include consumption of animal source foods to increase the intake of nutrients

    Determinants of urban and peri-urban youth employment in agribusiness in Malawi

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    Masters ThesisAgribusiness has been recognized to provide employment opportunities for youth in Malawi. However, little is known on factors that drive urban and peri-urban youth into agribusiness employment. This study examined the determinants of urban and peri-urban youth employment in agribusiness in Malawi. A cross-sectional study design was adopted. Sample size was 9680 youth for quantitative data while a sample of 135 for qualitative data. Quantitative data was obtained from nationally representative Living Standards Measurement Surveys - Integrated Household Survey (LSMS- IHS) data for Malawi and Regression analysis was used with the aid of STATA 14.0. Qualitative data was generated from focus group discussions and key informant interviews and the data was analysed using thematic analysis. The study found that 44.5% of youth were employed in agribusiness with 35.7% of them working in farming. Furthermore, the study found that age, access to credit, extension services and livestock ownership positively and significantly influenced youth employment in agribusiness (p < 0.05). Whereas, marital status, education level, household size, dependency ratio, wealth, and distance to the market negatively and significantly influenced youth employment in agribusiness (p < 0.01 and p < 0.05). However, in the multinomial logit factors that influence youth employment into specific agribusiness categories vary. Thematic analysis confirm that the above factors influence youth to be in agribusiness. Moreover, findings revealed that despite opportunities in agribusiness, youth face challenges such as lack of access to credit, extension services, limited agribusiness education (training), lack of improved agricultural inputs and lack of access to land and markets among other things. Furthermore, results from Ordinary Least Square (Multiple Linear) Regression found a negative significant relationship between being employed in farming and per capita consumption expenditure at p < 0.01. Whereas, there was a positive significant relationship between being employed in a mix of farming and non-agricultural business as well as being employed in off-farm agricultural activities with per capita consumption expenditure at p < 0.01. Generally, it can be concluded that youth employment in agribusiness is influenced more by push factors, which highlights the lack of government investment and support towards youth in agribusiness and the sector itself. The study recommends that implementation of policy pathways should be potentially geared towards improving access to credit, agricultural extension services, and education in agribusiness, improved agricultural inputs, land and markets among other things. This will promote youth employment in agribusiness and thus, improve well-being of youth.Prof. Mark Mwandosy

    Increasing maize yields with soil testing and subsides in Tanzania

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    Though the use of fertilizers can enhance productivity and increase profits for small-scale farming families, two barriers to their adoption have proved critical. First, many farmers often cannot afford to buy fertilizers; second, use recommendations from government sources may not be appropriate for specific farms. In Tanzania, 1 our multi-disciplinary team used low-cost soil testing to pair field-specific recommendations with subsidy vouchers to help cover the cost of fertilizer purchase. Our results show that farmers benefitted significantly in both yields and profits when (1) fertilizer recommendations were calibrated to the needs of individual farms and (2) a subsidy made the recommended fertilizers affordable

    Optimization of in-vitro regeneration of pineapple (ananas comosus (l) merr)

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    Master ThesisThe objectives of this study were to determine the optimum concentrations of 6- Benzylaminopurine (BAP) and Naphthalene Acetic Acid (NAA) and Murashige and Skoog (MS) media state on in vitro multiplication of local pineapple cv. “Smooth Cayenne”. Experiments were carried out at Mikocheni Agricultural Research Institute from September 2016 to July 2017. In the first experiment, crown and ratoon derived explants were cultured in MS medium supplemented with BAP at 0, 2, 3, 4, 5, 5.5mg/L in combination with NAA at 0, 0.01, 0.05, 0.1 and 2mg/L while in the second experiment, ratoon and crown derived explants were cultured in MS semi-solid, solid and liquid media. MS semi-solid and solid media were prepared by adding 1.25 and 2.5g/L of phytagel as gelling agent. The pineapple explant were divided into four portions, planted in MS media with different BAP and NAA combinations and then incubated in a growth room at temperature of 26` ± 2 °C and photoperiod of 16h dark and 8h light per day. Data on number of plantlets per quarter explant and weight of plantlets at 16th week from the date of culture initiation were collected. Data were analyzed using Gen Stat Computer Software version 12th based on split plot in a completely randomized design, and treatment separation was performed based on Duncan Multiple Range Test at p ≤ 0.05. Crown derived explants cultured in BAP at 5mg/L and NAA at 2mg/L produced the highest number of 16 plantlets per quarter explant each weighing 1.979g. Moreover, crown derived explants cultured in semi-solid MS media produced the largest number of plantlets of 16.33 plantlets per quarter explant each weighing 1.966g. It is recommended that tissue culture propagators should improve in vitro propagation of pineapple by culturing crown-derived explants in MS semi-solid media supplemented with BAP at 5mg/L and NAA at 2mg/L. Further studies are required to determine the effect of crown and ratoon-derived seedlings on field performance of pineapple.Commission for Science and Technology (COSTECH

    Comparison of body length-weight relationship and condition factor for Nile tilapia (Oreochromis niloticus) cultured in two different climatic conditions in Tanzania

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    This study compared length-weight relationship and condition factor of male Nile tilapia raised in two districts experiencing different climatic conditions in Tanzania, Mbarali and Mufindi. Fish were raised for six (6) months and measurements were done biweekly. The results suggests negative allometric growth pattern (b 99%) between length and weight of the fish. The condition factor (K) ranged from 2.74 to 3.50 in Mbarali and 1.96 to 2.40 in Mufindi, throughout the experimental period. Significantly higher condition factor was observed for fish grown in Mbarali (3.168 ± 0.056) than those in Mufindi (2.166 ± 0.056). It is concluded that, the climate of Mbarali provides better conditions for growth of fish than that of Mufindi

    Functional disabilities and associated factors among elderly people in Misungwi District, Mwanza

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    Partial Dissertation MSc. In Human NutritionThe older population is increasing worldwide and in many countries older people will outnumber younger people in the twenty first century. Change in age structure among the elderly is associated with functional disabilities by limiting performance of daily activities. . Functional disabilities increase dependency and institutionalization, also they are associated with higher mortality among elderly people. This study was carried out to assess functional disabilities in activities of daily living and associated factors among institutionalized and free living elderly in Misungwi district, Mwanza Tanzania. A cross- sectional research design was adopted involving a total of 120 elderly people in which 60 respondents were from Bukumbi elderly centre, 30 were randomly selected from Igokelo ward and 30 from Mabuki ward. A questionnaire-based survey was used to collect information and data were analysed using SPSS version 20, descriptive statistics was computed, independent t-test was performed to test median score difference and chi- square test was performed to determine association and for comparison between two groups. The study found a significant difference (p=0.0489) on the most leading functional disability since about a half (51.7%) of institutionalized elderly revealed mobility as their leading disability while in non-institutionalized respondents the leading disability was toileting (48.3%). In terms of gender, findings revealed that toileting disability was more common among males from both institutionalized and non- institutionalized respondents with 46.3% and 55.5% respectively while mobility disability was common among female respondents from both institutionalized and non- institutionalized with 56.2% and 66.7% respectively. Results of daily energy intake estimated from 3 food record was significantly higher (p=0.03) among institutionalized elderly (1632.3g) than non-institutionalized elderly (1317.0g). Also results showed significant difference (p=0.0182 ) in terms of nutritional status since more than a half (58.1%) of institutionalized respondents showed high prevalence of normal status while in non-institutionalized elderly, about a half (53.7%) showed high prevalence in underweight. In terms of chronic diseases, majority of institutionalized respondents reported leprosy (40%) as their main chronic diseases while majority of non- institutionalized respondents reported arthritis (43.8%) as their main chronic diseases. In conclusion, institutionalised elderly appeared to be better off than their counterparts in terms of daily energy intake, nutritional status reflected by their BMI levels and access to health services and chronic diseases were more prevalent in females than males

    Arbovirus infections in aedes mosquitoes at the interface of human-livestock-wildlife ecosystem in Kilosa district, Tanzania

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    Masters ThesisArboviruses refer to a group of viruses, which are transmitted by arthropods including mosquitoes and ticks. The objective of this study was to determine mosquito transmission potential of arboviruses at the interface of human-livestock-wildlife ecosystem in Kilosa district, Tanzania. Adult mosquitoes were collected using Mosquito Magnet® Liberty Plus traps. Reverse transcription polymerase chain reaction assay was performed on pooled adult Aedes mosquitoes to detect the presence of Dengue virus (DENV), Chikungunya virus (CHIKV), Rift Valley fever virus (RVFV) and Yellow fever virus (YFV). A total of 1340 mosquitoes belonging to four genera (Aedes, Anopheles, Culex and Mansonia) and 6 species were collected. Culex accounted for the largest (48.06%; n= 644) proportion of the mosquitoes while Anopheles for the lowest proportion (2.54%; n=34). Of the total mosquitoes collected, Aedes aegypti accounted for the majority of mosquito species (46%; n=613), followed by Culex quinquefasciatus (44.1%; n=591). Of the 36 Ae. aegypti pools tested for arbovirus 10 (28%) pools were positive. Dengue virus was detected in 3% (1/ 36) pools and CHIKV in 25% (9/ 36) pools. One pool that was positive for DENV also tested positive for CHIKV indicating the possibility of co-infection whereby, individuals may become infected by more than one arbovirus at a time thus risk of co-transmission to human and livestock. Nucleotide sequencing of polymerase chain reaction (PCR) products of the structural polyprotein region of DENV produced 511 bp fragment. Basic Local Alignment Search tool for nucleotides (BLASTn) and phylogenetic analysis showed that the (DENV3/TAN/Mikumi/2020 strain) (Accession number MW133786) obtained from this study clustered with DENV-3 strains reported in China and Kenya. This information is important as it gives knowledge on areas at high risk for arboviral disease outbreaks. The findings indicate that the presence of various mosquito vectors and detection of arboviruses in wild-caught Aedes mosquitoes leave the population of Kilosa district at a higher risk of transmission of DENV and CHIKVSACID

    From pests to tests: training rats to diagnose tuberculosis

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    Journal of Eur Respir J 2020; 55: 19022432020 is the year of the rat. The rat is the first of the 12 Chinese zodiac signs, and represents spirit, alertness, flexibility and vitality. In respiratory medicine, we may think of rats as vectors for diseases, such as pulmonary forms of hantavirus disease or leptospirosis, and pneumonic plague. Rodent control is thus part of hygiene guidelines and the International Health Regulations. And yet, the rat’s keen sense of smell has led to its incredible career as a living tuberculosis (TB) detector. The TB detection rat journey began with an idea in Tanzania in 2001, roughly 120 years after the TB-causing Mycobacterium tuberculosis was discovered by microscopy and bacterial culture. African giant pouched rats (Cricetomys ansorgei) had already been successfully trained using positive reinforcement to find explosives [1]. So, the question arose if they could do more for humanity by helping to also combat disease. But how would the rats use their noses to find bacteria or otherwise sniff out TB patients? For centuries, olfaction had already informed diagnostics in medical practices, e.g. in detecting metabolic issues such as ketoacidosis or tissue decay such as gangrene [2]. In the Flemish common language, the word tering, which etymologically refers to the smell of tar, is used for TB. The advent of chromatographic techniques allows characterisation of the organic compounds causing odours

    The Coverage of Biodiversity Conservation Issues in Local Newspapers and Its Role in Enhancing Conservation Awareness in Tanzania: An Analysis of Two News Outlets

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    noneReview on the coverage of biodiversity issues in local newspapers was conducted between 2004 and 2013 to understand the importance given to the conservation news by two local newspapers. Twelve biodiversity issues were covered at varying scales, both from rural and urban areas. Reports on biodiversity issues showed a fluctuating but with increasing trend. The reporting of biodiversity issues was overall higher than the coverage of socio-political issues during the studied period. However, both newspapers reported very few research- based news (<5% of all reports) and some news were incorrectly presented, suggesting inadequate knowledge on conservation issues among the journalists and a limited interaction between the media and scientists. Conservation education to journalists and improved interaction and information sharing between scientists and journalists are critical factors in improving the quality of biodiversity conservation news flowing to the general public and policy makers.privat

    Validation of the Perceived Self-efficacy Scale (PSE) Among Cassava Farmers in Tanzania

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    Journal of Education, Humanities and Sciences, Volume 9 No. 1 , 2020: pp. 18–34This paper validated the Perceived Self-Efficacy (PSE) scale as a measurement tool for farmers’ self-efficacy. From the social cognitive theory, self-efficacy is the key determinant of many social-cognitive behaviours, including the adoption of farming technologies. In this paper, PSE was adopted and tested in a two-stage study among cassava farmers in Mara, Mwanza, and Kagera regions of Tanzania. In the first stage, 200 respondents in Serengeti District of Mara region were exposed to the 13 items of PSE scale, while in the second the adopted tool with 11 items was administered to a total of 360 participants in Mara, Mwanza, and Kagera regions. The Principle Component Analysis (PCA) was performed, which was later followed by the Monte Carlo Parallel analysis. Results indicate that the 11 items of the PSE were relevant in measuring farmers’ self-efficacy. Further, the moderate discriminant validity (r = 0.48), and good internal consistency (α = 0.85) of the adopted PSE were found. It was concluded that PSE is an effective instrument in assessing individual differences in perceived self-efficacy. The study recommends the adoption of PSE as an effective tool for measuring farmers’ self-efficacy

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