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シロギス仔稚魚の体型と形態異常に関する研究：天然個体と人工生産された個体との比較
Full text link長崎大学学位論文 [学位記番号]博（水・環）甲第105号 [学位授与年月日]令和7年2月19日(2025-02-19)thesi
Analysis for type of 53BP1 nuclear expression by immunofluorescence as an indicator of genomic instability in oropharyngeal squamous epithelial lesions
Full text linkNagasaki University (長崎大学)博士(医学)A subset of oropharyngeal squamous cell carcinoma (OPSCC) is caused by the high-risk human papilloma virus (HPV), which expresses p16INK4a immunoreactivity. Dual-color immunofluorescence (IF) analysis of TP53 binding protein-1 (53BP1) and a proliferative indicator, Ki-67, to elucidate genomic instability (GIN) in tumor tissues revealed that abnormal 53BP1 expression is closely associated with carcinogenesis in diverse organs. We have previously demonstrated that the number of 53BP1 nuclear foci (NF) in cervical cells increases with cancer progression. The distribution of 53BP1 NF was similar to that of punctate HPV signals, as determined by in situ hybridization, and the pattern of p16INK4a overexpression. The present study aimed to confirm the type of 53BP1 expression using dual-color IF as an indicator of GIN in oropharyngeal squamous epithelial lesions, including HPV-dependent and -independent OPSCC. This study identified significant differences in the nuclear expression of 53BP1 between benign oropharyngeal epithelial lesions and OPSCC, and between HPV-dependent and HPV-independent OPSCC. We concluded that the incidence of abnormal 53BP1 expression in OPSCC is significantly associated with stage classification and overall survival. Therefore, double IF analysis of 53BP1 and Ki-67 expression may be a useful tool for estimating the malignant potential and prognosis of OPSCC.長崎大学学位論文 学位記番号:博(医歯薬)甲第1663号 学位授与年月日:令和7年3月5日Author: Hideaki Nishi, Katsuya Matsuda, Mariko Terakado, Hisayoshi Kondo, Yoshihiko Kumai & Masahiro NakashimaCitation: scientific reports, 14, art. no. 27525; 2024Nagasaki University (長崎大学), 博士(医学) (2025-03-05)doctoral thesi
Anti-tumor Effects of Erlotinib via Thymidylate Synthase Down-regulation in Pancreatic Cancer Cells
Full text linkNagasaki University (長崎大学)博士(医学)Background/Aim: In pancreatic cancer, gemcitabine and EGFR tyrosine kinase inhibitors (EGFR-TKIs) are common chemotherapy options. Reports have shown that EGFR-TKIs suppress the expression of thymidine synthase (TS), an important enzyme for DNA biosynthesis, and increase sensitivity to gemcitabine in lung cancer. However, no such reports have been made in pancreatic cancer. Materials and Methods: Human pancreatic cancer cell lines MiaPaCa2, Panc1, and BxPc3 were used. TS mRNA and protein expression levels in the cells were analyzed after erlotinib treatment. In addition, the anti-tumor effect of TS knockdown was verified using TS siRNA, along with its synergistic effect when combined with gemcitabine. Results: TS expression was high in MiaPaCa2 and Panc1 cells and low in BxPc3 cells. After erlotinib treatment, TS mRNA and protein levels decreased markedly in MiaPaCa2 cells dose-dependently, but not in Panc1 cells. TS siRNA caused specific down-regulation of TS in MiaPaCa2 and Panc1 cells. TS down-regulation resulted in an anti-tumor effect in these cells (MiaPaCa2 42%; Panc1 38%; p<0.05), showing a synergistic effect when combined with gemcitabine. Conclusion: Erlotinib could have a synergistic anti-tumor effect when combined with gemcitabine via down-regulation of TS expression.長崎大学学位論文 学位記番号:博(医歯薬)甲第1664号 学位授与年月日:令和7年3月5日Author: SATOSHI TABUCHI, TETSURO TOMINAGA, TOMOSHI TSUCHIYA, RYOICHIRO DOI, JYUNICHI ARAI, TAKASHI NONAKA, TAKURO MIYAZAKI, TATSUO INAMINE, KAZUHIRO TSUKAMOTO and KEITARO MATSUMOTOCitation: Anticancer Research, 45(2), pp.503-510; 2025Nagasaki University (長崎大学), 博士(医学) (2025-03-05)doctoral thesi
18F-fluorodeoxyglucose positron emission tomography/computed tomography parameters are associated with histological outcomes in superficial esophageal squamous cell carcinoma
Full text linkNagasaki University (長崎大学)博士(医学)Endoscopic submucosal dissection is a standard treatment for early esophageal squamous cell carcinoma. However, submucosal or lymphovascular invasion increases the risk of lymph node metastasis. Although 18F-fluorodeoxyglucose positron emission tomography/computed tomography (FDG PET/CT) parameters are associated with prognosis in patients with advanced esophageal squamous cell carcinoma, the utility of FDG PET/CT in diagnosing superficial esophageal carcinoma remains unclear. This study aimed to investigate the association between FDG PET/CT parameters and histopathological findings in superficial esophageal carcinoma. Fifty-three patients with superficial esophageal cancer who underwent FDG PET/CT scans before undergoing interventions were retrospectively analyzed. The maximal standardized uptake value (SUVmax), metabolic tumor volume, and total lesion glycolysis were significantly higher in the cases with submucosal invasion (T1b) compared with those confined to the muscularis mucosa (T1a). In contrast, classification of intrapapillary capillary loops patterns with magnifying endoscopy did not yield statistical differences between T1a and T1b. Multivariable analysis revealed that SUVmax was the only independent predictor of submucosal and lymphovascular invasion. This study demonstrated that SUVmax may be useful in predicting submucosal and lymphovascular invasion. Thus, the value of SUVmax may guide clinical decision-making in superficial esophageal squamous cell carcinoma.長崎大学学位論文 学位記番号:博(医歯薬)甲第1682号 学位授与年月日:令和7年3月19日Author: Hisaya Tanaka, Keiichi Hashiguchi, Maiko Tabuchi, Arman Nessipkhan, Taro Akashi, Junya Shiota, Moto Kitayama, Kayoko Matsushima, Naoyuki Yamaguchi, Junichi Arai, Kengo Kanetaka, Masahiro Nakashima, Takashi Kudo, Kazuhiko Nakao & Yuko AkazawaCitation: Scientific Reports, 14, art. no. 17493; 2024Nagasaki University (長崎大学), 博士(医学) (2025-03-19)doctoral thesi
Longevity of retained deciduous teeth: A retrospective cohort study
Full text linkNagasaki University (長崎大学)博士(歯学)Objective: This study aimed to investigate the factors related to the survival of retained deciduous teeth (RDTs). Materials and methods: RDTs were identified in panoramic X-ray images in 13,516 patients at Nagasaki University Hospital from 2014 to 2018. The survival status of the RDTs was tracked using medical records; survival rates were calculated. The RDTs were divided into two groups: before and after the completion of stable permanent dentition. A shared frailty analysis was performed in each group, estimating hazard ratios and 95% confidence intervals for variables related to survival. Results: A total of 138 patients (50 male and 88 female participants, 1.02%) had RDTs. The total number of RDTs was 274, with survival rates of 62.48% at 10 years and 53.30% at 20 years. Before the completion of permanent dentition, the longevity of RDTs was associated with two variables: tooth type (anterior/molar) and presence or absence of successive permanent teeth. No variables were associated with RDT survival after permanent dentition was stabilized. Conclusion: Multiple congenital factors affect the longevity of RDTs before the stabilization of permanent dentition. However, no significant influence was observed based on factors identified retrospectively after the permanent dentition was stabilized.長崎大学学位論文 学位記番号:博(医歯薬)甲第1687号 学位授与年月日:令和7年3月19日Author: Risako Kinoshita, Naomi TanoueCitation: Pediatric Dental Journal, 34(3), pp.152-157; 2024Nagasaki University (長崎大学), 博士(歯学) (2025-03-19)doctoral thesi
TIGIT expression on neoplastic cells is a poor prognostic factor for adult T-cell leukaemia/lymphoma
Full text linkNagasaki University (長崎大学)博士(医学)Adult T-cell leukaemia/lymphoma (ATLL) is an aggressive peripheral T-cell neoplasm with a poor prognosis. T-cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domains (TIGIT) is an immune checkpoint receptor expressed on T and natural killer cells. Although increased TIGIT expression in the tumour microenvironment is associated with poor prognosis in various neoplasms, its relevance in ATLL remains unknown. Herein, we investigated the clinicopathological impact of TIGIT expression on ATLL using immunohistochemistry. TIGIT expression was detected in 21 of 84 patients (25%). A partial association between the clinical features and immune checkpoint molecules and the expression of TIGIT was found including sIL-2R, CD86 and GITR. TIGIT-positive patients [median survival time (MST) 8.9 months, 95% confidence interval (CI) 7.7–15.6] had inferior overall survival compared with TIGIT-negative patients (MST 18.7 months, 95% CI 12.0–36.4) (p=0.0124]. TIGIT expression maintained its prognostic value for overall survival in both univariate and multivariate analyses [hazard ratio (HR) 1.909; 95% CI 1.044–3.488; p=0.0356]. Further studies are required to clarify the clinical and biological significance of TIGIT expression in patients with ATLL.長崎大学学位論文 学位記番号:博(医歯薬)甲第1703号 学位授与年月日:令和7年3月19日Author: YUICHI YAMADA, HIROAKI MIYOSHI, MAI TAKEUCHI, KAZUTAKA NAKASHIMA, KYOHEI YAMADA, TAKEHARU KATO, KEN TANAKA, KEI KOHNO, YOSHITAKA IMAIZUMI, YASUSHI MIYAZAKI, KOICHI OHSHIMACitation: Pathology, 56(7), pp.993-999; 2024Nagasaki University (長崎大学), 博士(医学) (2025-03-19)doctoral thesi
Numerical Simulation on Dynamic Response of Drilling Parameters in Loaded Rock Mass
Full text linkThe characterization of the mechanical parameters of rock mass is a basic problem in the field of rock mechanics, and it is also an important basis for surrounding rock classification, stability analysis, and support design in underground engineering. Based on the engineering background of pressure relief drilling in Guotun Coal Mine, this paper carries out an engineering test while drilling. The numerical simulation method is used to explore the influence of different lithology rocks, different control conditions, and different confining pressure conditions on the parameters while drilling and to study the variation in drilling time, drilling depth, drilling rate, and revolution speed. The results show that under the same control conditions, the drilling rate of coal, mudstone, sandy mudstone, and siltstone are in the order of coal > mudstone > sandy mudstone > siltstone. For similar rock specimens, when the thrust is fixed, the drilling rate increases with the increase in the revolution speed, and when the revolution speed is fixed, the drilling rate increases with the increase in the thrust. When the rock specimen is in different confining pressure states, the drilling rate decreases with the increase in confining pressure, and the torque increases with the increase in confining pressure. This study provides a scientific basis for the realization of in situ rapid and effective measurement technology for the rock mechanical parameters of coal and rock mass, which is helpful for improving the measurement accuracy and efficiency and promoting the safe and efficient mining of coal mines.Applied Sciences, 15(11), art. no. 5977; 2025journal articl
Short-Term Administration of Parathyroid Hormone Improves Wound Healing Around Implants in an Osteoporotic Rat Model
Full text linkBackground/Objectives: Intermittent parathyroid hormone (PTH) administration increases bone quantity. Existing animal studies have revealed improvements in tissue healing around implants after PTH administration. It is still unclear whether PTH has a beneficial short-term effect on the early healing of bone and soft tissue around implants in individuals with osteoporosis. The current study aims to examine whether short-term intermittent PTH administration accelerates and improves early tissue healing around implants in ovariectomized rats. Methods: Ovariectomized rats received implants at the healed sites of extracted maxillary first molar sockets 12 weeks after the ovariectomy surgery. A daily dose of PTH was subcutaneously administered in the test group, whereas saline was administered for the control group. Long bones and maxillae were harvested 1 week after PTH administration. The following criteria were assessed: quantity and quality of long bones and peri-implant bone, bone healing around the implants, and soft tissue healing. Results: PTH significantly increased the bone parameters of long bones. Moreover, the bone volume around the implant increased significantly compared to controls. Improved bone quality was indicated through PTH administration by increased numbers of osteoblasts and osteoclasts, as well as decreased quantities of sclerostin + osteocytes. Furthermore, PTH administration significantly improved soft tissue healing, promoted collagen production and angiogenesis, and increased the numbers of macrophages in the connective tissue around the implants. Conclusions: Short-term intermittent PTH administration significantly accelerates soft tissue healing, which could lead to enhanced early osseous healing and bone formation around implants. Thus, Intermittent PTH administration might be considered as an available treatment modality for dental implants in osteoporosis patients.Journal of Clinical Medicine, 14(11), art. no. 3900; 2025journal articl
Projected compound effects of population aging and climate warming on emergency ambulance demand in Japan
Full text linkBackground: The emergency medical services system, including emergency ambulance dispatch (EAD), faces significant challenges due to a rapidly aging population and climate change. Notably, despite the warming climate, it remains unclear whether the high EAD incidence in winter will persist. This study projects the compound impact of these factors on future seasonal demands of EAD in Japan. Methods: We analyzed 26,595,933 all-cause EAD cases among older adults in Japan in 2011–2019 and projected EAD incidence in 2011–99 under climate-population scenarios. We compared EAD incidence and its seasonality between 2011 and 2019 and future decades. Peak-to-trough ratio (PTR) and attributable fraction (AF) were used to measure the amplitude and burden of seasonality. Results: Until mid-century, annual incidence cases are projected to rise by 15 %, followed by a decline afterwards. The annual incidence rate is expected to increase by 2.95 % to 4.25 % by the 2090s under various scenarios. Importantly, the high-incidence peak is projected to shift from cold to warm season, while high incidence in cold season persists. PTR and AF are projected to increase, with more profound changes under extreme climate scenario. Conclusion: Demand for emergency medical services among older adults is expected to rise, especially until mid-century, with high demand in both summer and winter. Adaptive strategies are needed to manage increasing emergency service demands and shifts in seasonal patterns.Environment International, 202, art. no. 109619; 2025journal articl