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Biodegradable Nanoparticles Encapsulating Murine Double Minute 2 siRNA to Treat Peritoneal Dissemination of Colon Cancer
Get PDFThe study aim was to apply murine double minute 2 (MDM2)-siRNA to a biodegradable siRNA delivery vector, ternary complex, for treating colorectal cancer peritoneal dissemination. The ternary complex containing MDM2-siRNA (MDM2-siRNA complex) was constructed by mixing MDM2-siRNA, dendrigraft poly-L-lysine, and γ-polyglutamic acid. Cellular uptake of the ternary complex and suppressive effect on MDM2-mRNA were determined in a mouse colorectal cancer cell line. Tumor-growth inhibition by the MDM2-siRNA complex was evaluated in peritoneal dissemination model mice. The MDM2-siRNA complex, with an approximately 177 nm particle size and −35 mV ζ-potential, prevented degradation of the inner siRNA by RNase. In the in vitro study, the ternary complex was highly taken up by the cells, and 2 μg/mL of the MDM2-siRNA complex significantly decreased MDM2-mRNA to about 30% of control cells. Intraperitoneal administration in colorectal cancer peritoneal dissemination model mice showed little effect of the ternary complex containing scramble-siRNA on cancer growth in the peritoneal cavity. Conversely, the MDM2-siRNA complex significantly reduced peritoneal dissemination to less than 1/1000th of control mice and successfully prolonged survival time. In this study, we found that the biodegradable MDM2-siRNA complex had a suppressive effect on MDM2-mRNA in cancer cells and tumor growth of peritoneal dissemination.International Journal of Molecular Sciences, 26(18), art. no. 8883; 2025journal articl
Association Between Dietary Exposures to Aflatoxins and Linear Growth Limitation: A Prospective Biomonitoring Study of Expectant Mothers and Newborn Cohorts in Kwale County, Kenya
Get PDF長崎大学学位論文 [学位記番号]博（Global Health）甲第19号 [学位授与年月日]令和7年9月19日(2025-09-19)thesi
Hypoxia- and mechanical stress–induced upregulation of mitochondrial HSP60 is associated with phenotypic switching of pulmonary arterial smooth muscle cells
Get PDF長崎大学学位論文 [学位記番号]共博（医歯薬）甲第56号 [学位授与年月日]令和7年9月19日(2025-09-19)thesi
Squamous metaplasia is an indicator of acute exacerbation in patients with usual interstitial pneumonia / idiopathic pulmonary fibrosis
Get PDF長崎大学学位論文 [学位記番号]博（医歯薬）甲第1717号 [学位授与年月日]令和7年9月3日(2025-09-03)thesi
The effect of ionizing radiation on testicular interstitial stromal cells
Get PDF長崎大学学位論文 [学位記番号]博（医歯薬）甲第1725号 [学位授与年月日]令和7年9月19日(2025-09-19)thesi
Research on Integrated Prediction of Drug Absorption from the Liver Surface
Get PDF長崎大学学位論文 [学位記番号]博（医歯薬）甲第1726号 [学位授与年月日]令和7年9月19日(2025-09-19)thesi
Stability Analysis of Deep Underground Structures Considering Rock Bolt Performance under Combined Tensile-Shear Loading
Get PDF長崎大学学位論文 [学位記番号]博（工）甲第163号 [学位授与年月日]令和7年9月19日(2025-09-19)thesi
Evaluation and simulation study on island ecotourism development
Get PDF長崎大学学位論文 [学位記番号]博（水・環）甲第111号 [学位授与年月日]令和7年9月19日(2025-09-19)thesi
Exploring Recent Japanese Public Perception of Freshwater Eels of the Genus Anguilla Using Content Analysis of Newspaper Coverage
Get PDFJapan has one of the highest consumption rates of freshwater eels, some of which are threatened by multiple factors, including anthropogenic activities. The level of public support for eel conservation should be affected by the Japanese public's perception of eels, but little is known about this. Here, we conducted a content analysis of newspaper coverage using 423 articles referring to freshwater eels published from 19 July 2020, to 28 July 2021, to examine aspects of eels that potentially motivate people to conserve them and people's perception of the eel population status. Our results suggest that Japanese people can be motivated to conserve eels for consumption, but some people appear to underestimate the population crisis because of shifting baseline syndrome; that is, people have become accustomed to a deteriorated population status. Of note, as interest in the eel catch, supply and abundance trends may increase around specific days, such as Doyo Ushi no Hi, on which most Japanese people conventionally consume eel dishes, educational activities on these days would be effective at reinforcing public support. It was found that experience with eels in nature appeared to decrease more drastically than experience with eel dishes, and this has been suggested as one of the factors encouraging the progress of shifting baseline syndrome. These results have implications for determining appropriate approaches to encourage Japanese society to conserve wild eel populations. However, our results were inferred from articles that might be potentially biased in content compared to public perception itself. Future studies are needed to verify our results and their implications using more robust methods and to examine the transition pattern of the Japanese public's perception of eels and the relevant factors affecting this perception.Aquatic Conservation: Marine and Freshwater Ecosystems, 35(7), art. no. e70164; 2025journal articl
Characteristics and Outcomes of Patients Who Underwent Curative Resection for Colorectal Cancer Local Recurrence
Get PDFBackground/Aim: The standard treatment for local recurrence (LR) is radical resection where possible, and R0 resection is reportedly associated with improved prognosis. However, the surgery for LR is complex and difficult, resulting in a low resection rate of 13-23%. The aim of this study was to examine clinical characteristics of LR and survival outcome after R0 resection of LR. Patients and Methods: We retrospectively reviewed the medical records of 18 patients who underwent curative surgery for colorectal cancer LR at four hospitals between April 2016 and March 2024. We examined the perioperative outcomes and prognosis of LR treated with R0 resection. Results: Pathological T4 was seen in 9 of 18 patients (50.0%), N-positive status in 12 (66.7%), and lymphovascular invasion in 15 (83.3%). Median interval from primary surgery to local recurrence was 24 months. Postoperative complications occurred in 9 patients (50.0%). Eight patients (44.4%) experienced re-recurrence, with peritoneal metastasis in 5 patients, liver metastasis in one, lung metastasis in one, and adrenal gland metastasis in one. Five-year RFS was 39.4% and 5-year OS was 52.2%. Conclusion: Postoperative LR of colorectal cancer is expected to show relatively favorable prognosis with R0 resection. LR often occurs within 3 years, and careful follow-up is necessary for high-risk cases.Cancer Diagnosis and Prognosis, 5(4), pp.478-484; 2025journal articl