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Penn Library\u27s LJS 403 - [Alchemical compendium]. (Video Orientation)
No full texthttps://repository.upenn.edu/sims_video/1155/thumbnail.jp
Collation Model for Ms. Codex 1602: [Notated office book] [manuscript].
Get PDFGerman manuscript containing texts and chants for the Office of the Dead (Officium pro defunctis), the funeral service for a priest, and the Divine Office. The lessons and responses in the Office of the Dead seem to be related to those used in a small group of manuscripts from the Diocese of Cologne, and the arrangement of the psalms for the Divine Office in the ferial psalter suggest that this manuscript was made for use by secular priests, such as members of a cathedral chapter, or Premonstratensian or Augustinian Canons (Les Enluminures). The volume concludes with the text for the Hours of the Virgin, whose use is unidentified but which contains a suffrage of Saint Nicholas (f. 64v), and a copy of Psalm 64 by a later hand (f. 65v).https://repository.upenn.edu/sims_models/1047/thumbnail.jp
Electrical Characterization of Solar Cell using Arduino and Polarization Film
Get PDFA solar cell laboratory course for high school and college undergraduate students is proposed. The electrical characterization of the solar cell is performed to confirm the functionality of the device as both a diode and a power source. The efficiency of the solar cell in the illuminated condition is found to be 13.1 %. We find the efficiency of the solar cell slightly decreases as the intensity of light decreases. However, there is no significant difference in efficiency except for in the opaque condition. A calibrated solar cell, multimeter, current sensor, Arduino, coding and polarization are used to perform the experiment.https://repository.upenn.edu/scn_educational/1002/thumbnail.jp
Collation Model for LJS 346: [Edicts concerning Jews in Mantua].
Get PDFOfficial copy of an edict issued by Vincenzo Gonzaga, duke of Mantua, in 1594 renewing privileges and amnesties of the Jews of Mantua, who were allowed to work as bankers, merchants, and butchers. The contemporary edict is preceded by copies of 2 earlier renewals, the first issued by Guglielmo Gonzaga, Vincenzo\u27s father and the previous duke, in Goito in 1587, and the second issued by Vincenzo in 1590. All 3 copies, perhaps written by a chancery scribe, are signed by ducal chancellor Matthaeus Gentilis.https://repository.upenn.edu/sims_models/1078/thumbnail.jp
Collation Model for LJS 56: [Logica parva].
Get PDFCompendium by the author of his own Logica magna, a presentation of terminist logic, including consideration of propositions and relationships between propositions and meaning. This early copy of this text was completed by the German Carmelite Johannes de Beylario, who was from Cologne and studied philosophy and theology in Padua, during the author\u27s tenure in Padua. Later in the century the Logica parva became a required element of the curriculum at Padua, Venice, and Ferrara.https://repository.upenn.edu/sims_models/1075/thumbnail.jp
Collation Model for Ms. Codex 1099: [Edict against reforming tendencies]
Get PDFEdict of 1 August 1528 (f. 5v) issued by Ferdinand I, king of Bohemia, against religious tendencies of the Reformation, in 23 numbered chapters or paragraphs, preceded by an introductory statement.https://repository.upenn.edu/sims_models/1086/thumbnail.jp
Characterizing And Quantifying The Redox Behavior Of Cytokine Hmgb1 In Drug-Induced Liver Injury Via High Resolution Mass Spectrometry
Get PDFHigh mobility group box 1 (HMGB1) is a protein that is released in several pathological contexts, including acetaminophen (APAP) overdose, and modulates the subsequent immune response to tissue damage. Oxidation of HMGB1 potentially regulates its interactions with the immune system but there have been a number of contradictory studies on the redox behavior of HMGB1 and what proteoforms of HMGB1 are present in oxidative pathology. Therefore, a stable isotope dilution two-dimensional nano-ultrahigh-performance liquid chromatography parallel reaction monitoring/high-resolution mass spectrometry (2D-nano-UHPLC-PRM/HRMS) method was developed along with a hepatocarcinoma cell model of APAP overdose to allow for characterization of HMGB1 redox behavior during APAP toxicity. Oxidative modifications to cysteine (Cys) residues (Cys-23, Cys-45, and Cys-106) that are present in HMGB1 were characterized and quantified using carbamidoethyl (CAE) derivatization before and after reduction as well as by direct analysis of disulfide cross-linked peptides. A stable isotope labeled form of HMGB1 was used as an internal standard to correct for sample-to-sample differences in immunoaffinity precipitation (IP), derivatization, and electrospray ionization. The metabolism, toxicity and release of HMGB1 in response to APAP were characterized in a cell model of APAP overdose. Four discreet HMGB1 proteoforms were found to be released from cells following a 24-hour exposure to toxic levels of APAP. Reduced HMGB1 with all three Cys-residues in their free thiol state accounted for 18% of the secreted HMGB1. The proteoform with disulfide between Cys-23 and Cys-45 accounted for 24% of the HMGB1. No evidence was obtained for a disulfide cross-link between Cys-106 and the other two Cys-residues. However, 45% of the HMGB1 formed a cross-link with unidentified intracellular proteins via an intermolecular disulfide bond and 12% was present as cysteic acid. Secreted plasma HMGB1 Cys-23/Cys45 disulfide proteoform together with the Cys-106/protein disulfide proteoforms could potentially serve as early biomarkers of hepatoxicity after APAP overdose as well as biomarkers of drug-induced liver injury (DILI)
Interrogating PTB--Associated Splicing Factor\u27s RNA Recognition Motif/RNA Binding-Mode
No full textPTB-associated splicing factor (PSF) is a multifunctional nucleic acid binding protein vital for cell survival. PSF plays several roles throughout the cell, yet many aspects of PSF’s mechanisms of interacting remain elusive. While protein and nucleic acid binding partners of PSF have been identified, binding consensus sequences reported in the literature vary. Previous studies in the Lynch Laboratory have found that PSF’s second RNA recognition motif (RRM2) is necessary and sufficient for binding, but this binding can be occluded by interacting with cofactor protein TRAP150. Additionally, binding is dependent on phosphorylation of PSF in T-cells. Identifying the interaction interfaces of PSF/RNA and PSF/TRAP150 yields insight into the regulatory mechanism governing RNA-binding. First, I utilize biophysical techniques to overcome challenges characterizing PSF/RNA. The smallest RNA to bind with low/modest affinity is 65-nucleotides long, making the protein/RNA pair non-ideal for crystallization or NMR experiments. PSF has long N- and C-terminal unstructured regions that aid in PSF’s functional aggregation, creating another challenge for biophysical characterization. Here I use several biophysical techniques to characterize PSF’s dynamics and binding interfaces. Mass spectrometry experiments indicate PSF contacts RNA using 30–60% of the accessible surface area. Interestingly, a charged hydrophobic loop region when mutated from “DDRGR” to “AAAAA” increases affinity for ESS-RNA 10-fold. A neighboring residue Lysine-413 also increases affinity for RNA when mutated to alanine. Arginine-474 and Threoinine-485 within the NOPS domain lose their ability to bind RNA when mutated. It is not clear whether these residues affect local conformation of playing a direct role in binding. Additionally, I identify hundreds of PSF-dependent alternative polyadenylation changes and begin characterization of PSF/TRAP150 and phosphorylated PSF by HDX-MS. Together, these results indicate PSF interacts with RNA using a large portion of the surface accessible area to do so, and PSF uses a loop in RRM2 to aid in RNA-interaction
Essays on Mechanism and Information Design
No full textThis dissertation consists of two essays that examine issues related to data—how data is generated, used and monetized. In Chapter 1, I study how intermediaries such as Amazon and Google recommend products and services to consumers for which they receive compensation from the recommended sellers. Consumers will find these recommendations usefulonly if they are informative about the quality of the match between the sellers’ offerings and the consumer’s needs. The intermediary would like the consumer to purchase the product from the recommended seller, but is constrained because consumers need not follow the recommendation. I frame the intermediary’s problem as a mechanism design problem inwhich the mechanism designer cannot directly choose the outcome, but must encourage the consumer to choose the desired outcome. I show that in the optimal mechanism, the recommended seller has the largest non-negative virtual willingness to pay adjusted for the cost of persuasion. The optimal mechanism can be implemented via a handicap auction. I use this model to provide insights for current policy debates. In Chapter 2, in the joint work with Mallesh Pai and Rakesh Vohra, we propose a statistical test for identifying whether a policy or an algorithm is designed by a principal with discriminatory tastes. The test can be used for identifying, for example, whether predictive policing algorithms are discriminatory against minority neighborhoods. We also argue that the marginal outcome test (Becker (1993)), the most popular test of taste-based discrimination, fails for policies. We consider a canonical setup where the principal designs a policy (algorithm) that maps signals (data) to decisions for each group, such as whether to patrol or not for each area. The principal commits to the policy, which in turn affects agents’ incentives to take action, such as whether to commit a crime. In this environment, the marginal outcome test fails because the principal not only cares about the marginal benefitof catching a criminal but how patrolling changes agents’ incentive to commit a crime. We propose a new statistical test that deviates from the marginal outcome test precisely as much as the incentive effect
Essays in Finance and Inequality
No full textStudents of lower-income families invest much less in college education than higher-income families. To assess the role of financing constraints and subsidy schemes in explaining this gap, I structurally estimate a model of college choice in the presence of financing frictions. The estimation uses novel nationally representative data on US high-school and college students. I propose a novel identification strategy that relies on bunching at federal Stafford loan limits and differences between in- and out-of-state tuition. I find that the college investment gap is mainly due to fundamental factors: heterogeneity in preparedness for college and the (perceived) value-added of college. Frictionless access to student loans would substantially increase consumption during college but would leave the investment in college education mainly unaffected. I show that making public colleges tuition-free would mitigate financing constraints, but it would overall entail more than $15B deadweight loss per year and would disproportionately benefit wealthier students. Expanding Pell grants, in contrast, would benefit lower-income students at a much lower cost