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River Sediments Downstream of Villages in a Karstic Watershed Exhibited Increased Numbers and Higher Diversity of Nontuberculous Mycobacteria
The impact of residential villages on the nontuberculous mycobacteria (NTM) in streams flowing through them has not been studied in detail. Water and sediments of streams are highly susceptible to anthropogenic inputs such as surface water flows. This study investigated the impact of seven residential villages in a karst watershed on the prevalence and species spectrum of NTM in water and sediments. Higher NTM species diversity (i.e., 19 out of 28 detected) was recorded downstream of the villages and wastewater treatment plants (WWTPs) compared to sampling sites upstream (i.e., 5). Significantly, higher Zn and lower silicon concentrations were detected in sediments inside the village and downstream of the WWTP's effluents. Higher phosphorus concentration in sediment was downstream of WWTPs compared to other sampling sites. The effluent from the WWTPs had a substantial impact on water quality parameters with significant increases in total phosphorus, anions (Cl-and N-NH3-), and cations (Na+ and K+). The results provide insights into NTM numbers and species diversity distribution in a karst watershed and the impact of urban areas. Although in this report the focus is on the NTM, it is likely that other water and sediment microbes will be influenced as well.O
Enriching absinthe bitters with stilbenes and lignans from waste plant materials
The study aimed to enrich absinthe bitters with stilbenes and lignans from waste plant materials, specifically Vitis vinifera and Picea abies. These bioactive compounds, analysed by liquid chromatography and gas chromatography, reached levels up to 580 +- 25 mg/L for stilbenes and 35.02 +- 1.21 mg/L for 7-hydroxymatairesinol. In primary absinthe, these values were below the detection limit. Total antioxidant capacity and polyphenol levels significantly increased, presenting the enriched absinthes as value-added products with enhanced bioactive content. Key aroma components remained unchanged, including terpenes fenchone and trans-anethole. Thujone concentrations complied with legal limits. The findings support the production of absinthe bitters with augmented bioactive substances without compromising key sensory attributes, offering a health-promoting alcoholic beverage.O
Overgrazing strongly impedes the natural regeneration of the endemic Boswellia species on Socotra Island
Frankincense trees (Boswellia spp.) worldwide are affected by a number of threats, including global warming and changing land management practices. On the Socotra Archipelago (Yemen), which harbours eleven endemic Boswellia species, grazing is generally assumed to be one of the main threats preventing natural regeneration. To test the impact of overgrazing on natural regeneration, we established an in situ experiment on four different Boswellia taxa in different areas of Socotra Island. Mortality and the height increment of seedlings were measured for a period of two/three years in five plots excluded from grazing (fenced) and in five paired control (unfenced) plots. Each plot was 50 m x 50 m in size and contained several adult trees as a source of viable seeds. Our results show that seedling mortality was significantly higher, and seedling height increment generally (4 out of 5 sites) lower in open compared to fenced plots. In the fenced plots, the number of seedlings for all species reached up to 772, with 560 surviving seedlings. In comparison, the control plots reached up to 296 seedlings, with 176 seedlings surviving after 2-3 years. The results of our experimental study indicate that grazing directly threatens the natural regeneration of the endemic Boswellia on Socotra Island. However, seedling mortality remained relatively high inside the exclosures as well, which indicates that even without the pressure of livestock grazing, other impacts remain a challenge for the future conservation of the archipelago's unique frankincense trees.O
Are there trade-offs between animal welfare and egg-producing farm efficiency?
Agribusiness is currently faced with the challenge of providing sufficient quality food for a growing population with limited natural resources. Egg production, as an important source of nutrition, is a dynamically developing sector of livestock production on a global scale. Simultaneously, the increasing public and legal considerations of animal welfare affect egg-producing operations. This paper aims to provide insight into missing evidence in the trade-offs between animal welfare and efficiency in egg-producing farms in the Czech Republic and the European Union. Detailed data were gathered on the extent of the enriched cage and indoor cage-free housing systems (aviaries/barns) among the main 30 Czech table-egg-producers. Original micro data enlarged the information on table-egg producers collected using the Orbis database. For the efficiency analysis at the EU level, data on the housing systems were obtained from Eurostat and combined with aggregated data from the Orbis database. A robust data envelopment analysis was applied which benefits from including animal-welfare variables directly into the efficiency evaluation. The results showed a negative, but statistically insignificant, relationship between the animal welfare and efficiency of the egg producers for the Czech Republic table-egg producers as well as those on the EU-country level.O
The effect of nitrogen-sulphur fertilizer with nitrification inhibitor on winter wheat (Triticum aestivum L.) nutrition
The high input of nitrogen is often required in today's agriculture, especially for the most cultivated crops largely involved in human and animal nutrition, such as winter wheat. Nitrogen is a mobile nutrient in the soil, and the high doses of N are often associated with possible losses through volatilization or leaching. One of the possible options to increase nitrogen use efficiency is the application of fertilizers with inhibitors. The main objective of the presented three-year experiment established under the field conditions at the two experimental sites was to examine the effect of nitrogen-sulphur fertilizer (ammonium nitrate sulphate) with the inhibitors of nitrification (IN) (dicyandiamide and 1,2,4 triazole). In addition to the nitrogen content in two forms, this fertilizer also contains sulphur, which can possibly enhance the utilization of nitrogen due to their well-known synergy. The treatments included in the experiment were: 1. Unfertilized, 2. N technology 3. N + S technology and 4. N + S + IN. The total dose of applied N for every fertilized treatment was 159 kg/ha. Treatments 2 and 3 were fertilized with three split doses of N, treatment 4 was fertilized only two times due to the addition of IN (a higher dose of fertilizer in the second application). The results obtained from the three-year experiment showed a significantly higher yield of grain (8.18 t/ha) after the fertilization with N + S + IN in comparison with N + S (7.67 t/ha) and N (7.61 t/ha), which proved the positive effect of IN on nitrogen use efficiency during the vegetation. The differences between qualitative parameters of wheat grain (hectolitre weight, protein and gluten content) were evaluated as statistically insignificant for each fertilized treatment. This similar result is likely due to the IN application, which provided a continuous nitrogen supply during vegetation comparable to the three split nitrogen applications. Thus, our results showed, that the addition of IN to the higher dose of fertilizer applied earlier in the vegetation can provide comparable results in terms of quality to the technologies based on three split fertilizations. The three-year experiment established at two experimental sites has proved, that the application of ammonium sulphate nitrate fertilizers with IN in a higher dose is a better option to the commonly used nitrogen technology, which was also supported by the economic evaluation and the highest net profit.O
Fragmentation and biodiversity change in urban vegetation: A case study of tram lines
This study explores the vegetation composition along various tram line sections in Bydgoszcz, Poland, with a focus on understanding the impact of usage, maintenance, and historical development on urban vegetation dynamics. This study addresses this knowledge gap by investigating how these factors influence plant species composition, leading to variations in the prevalence of native and non-native species. The aim of this study is to contribute to urban ecological knowledge and inform vegetation management strategies. The investigation centers on five tram line sites representing different usage and maintenance scenarios: Unused line, New line, Loop rec, Loop old, and Old line. Through phytocoenological relevés conducted in 2020 and 2021, we measured the plant taxa coverage. Detrended Correspondence Analysis (DCA) and Canonical Correspondence Analysis (CCA) revealed relationships between plant species and tram line sites. The results indicated the presence of 107 plant taxa, with graphical representations highlighting the prevalent species on each tram line section. Older sections showed a higher representation of non-native plant species, whereas new sections exhibited native species dominance. Moisture-indicative values suggest an affinity for soils with average moisture content. CCA provides insights into the relationships between plant taxa and site variants and offers valuable implications for urban vegetation management and conservation strategies. The novelty of this study lies in its holistic approach for understanding how multiple factors interact to shape urban vegetation. By illuminating urban ecological dynamics and informing decision-making processes, this study contributes to urban planning, ecology, and biodiversity conservation. Additionally, this study fills a knowledge gap by providing insights into the unique ecological dynamics and species composition of tram lines in urban environments. Unlike previous research in Central European countries focusing on planted vegetation, this study explored the spontaneous spread of plants and successional patterns along tram lines, enhancing our understanding of the environmental conditions created by tram lines that influence vegetation composition and development.OA-hybri
Assessing the Influence of Social Factors on Local Perceptions of Climate Change, Product Value Addition, Multiple Uses of NTFPs, and Their Influence on Poverty Alleviation in Ghana
The key to the successful and sustainable utilisation of non-timber forest products (NTFPs) by local communities lies in understanding their perspectives on climate change and its effects on NTFP production. Furthermore, assessing their perceptions of the diverse uses and potential for the value addition of NTFPs is crucial for determining how these resources can enhance the well-being of local livelihoods. Although studies on climate change, value addition, and the multiple uses of NTFPs and their impact on local livelihoods have been conducted, locals' perceptions of these factors have not been fully explored. This study aims to assess various social factors, including education, gender, and religion, and their influence on locals' perceptions of the abovementioned factors. The research focused on five regions where NTFPs are prevalent in Ghana (the Western North, Bono, Ahafo, Ashanti, and Eastern regions). To investigate the influence of social factors on local perceptions of climate change, value addition, and the multiple values of NTFPs within rural communities, a total of 732 locals were randomly selected with a team of interviewers across these five regions in Ghana. Key informant interviews and focus group interview methods were used for the data collection. We applied a logistic regression model to assess the social factors and their influencing locals' perceptions of the mentioned variables. It was revealed that age, gender, education, and occupation did not significantly influence locals' perceptions of climate change. However, religion was found to influence locals' perceptions of climate change. Furthermore, the results demonstrated that gender and education strongly influenced locals' perceptions of value addition to NTFPs. In contrast, religion and constraints (related to time, finance, and skills) did not significantly influence locals' perceptions. In conclusion, this study provides valuable insights into the intricate interplay among local perceptions, climate change, value addition, multiple uses of NTFPs, and the capacity of NTFPs to enhance the well-being of forest fringe communities. These findings underscore the importance of comprehensive, tailor-made interventions that consider local perspectives and adhere to sustainable approaches, with the aim of optimising the positive impacts of NTFPs in poverty alleviation and overall community development.O
A comprehensive investigation of geoenvironmental pollution and health effects from municipal solid waste landfills
This study investigates human health risks associated with heavy metals (HMs) occurrence in municipal solid waste (MSW) landfills. For testing of selected MSW landfills steps were involved, including site characterization, soil sampling and chemical testing, statistical analysis, as well as health risk assessment, carcinogenic and non-carcinogenic effects. For the Polish landfill (Radiowo) the average HMs concentrations were found in the following order: Zn (52.74 mg/kg DM) > Pb (28.32 mg/kg DM) > Cu (12.14 mg/kg DM) > Ni (4.50 mg/kg DM) > Cd (3.49 mg/kg DM), while for the Czech landfill (Zdounky): Zn (32.05 mg/kg DM) > Cu (14.73 mg/kg DM) > Ni (4.73 mg/kg DM) > Pb (0.10 mg/kg DM) = Cd (0.10 mg/kg DM). Strong positive correlations between selected HMs demonstrated identical origins. Principal component analysis (PCA) performed for the Radiowo landfill transferred the soil parameters into three principal components (PCs), accounting for 87.12% of the total variance. The results of the PCA analysis for the Zdounky landfill revealed three PCs responsible for 95.16% of the total variance. The exposure pathways of HMs for landfills were in the following order: ingestion > dermal absorption > inhalation. For both landfills, the values of hazard quotient were lower than 1, indicating no potential negative health effects. In terms of the hazard index (HI), for both landfills, no adverse human health effects occur (HI < 1). The incremental lifetime cancer risk (ILCR) values indicated negligible or acceptable carcinogenic risk of HMs (average ILCR in the range from 5.01EMINUS SIGN 10 to 5.19EMINUS SIGN 06).OA-hybri
The palaeoclimatic potential of recent oak tree-ring width chronologies from southwest Ukraine
Better insights into spatio-temporal climate signals are needed to understand more clearly the applicability to palaeoclimatic analysis and dendrochronological dating of the long tree-ring oak chronologies currently being compiled in Eastern Europe. This study investigates the climate sensitivity of two recent oak tree-ring width (TRW) chronologies from Transcarpathian and Ciscarpathian Ukraine and their coherence with 35 oak chronologies from Ukraine, Poland, Slovakia, Romania, and Hungary. The new Transcarpathian chronology consists of 247 TRW series of living trees from 13 sites covering the period 1836-2020, while the new Ciscarpathian chronology consists of 215 TRW series from 13 sites and spans the period 1775-2020. Despite the strong similarity between these two chronologies, their responses to climate differ significantly. Growing-season precipitation and particularly drought (three-month SPEI index) were found to be the primary drivers of oak growth on the border between the Carpathians and the northeastern Pannonian Basin. Spatial correlations of the Transcarpathian chronology show particularly high explained variability in the April-August SPEI index, roughly between 18.5-28.5oE and 45-52oN. In the Ciscarpathian, June precipitation primarily influenced oak radial growth but the spatial correlation was quite low. While the Transcarpathian TRW chronology was strongly correlated with eastern Slovakian and northwestern Romanian chronologies, the Ciscarpathian chronology revealed very low correlations with surrounding chronologies. This study indicates the great dendroarchaeological and palaeoclimatic potential of the Transcarpathian chronology and points to the need to analyse additional living trees from the Ciscarpathian region to understand the spatial variability of oak growth and its climate signal better.Preprin
Element contents and their seasonal dynamics in leaves of alder Alnus glutinosa (L.) Gaertn
Alnus glutinosa is an actinorhizal plant that fixes N via actinomycetes. Compared to other trees, A. glutinosa is more resistant to environmental stress and able to uptake soil nutrients more easily. Alnus glutinosa grows well not only in natural stands but also in degraded environment or soil in need of restoration. Changes in the contents of selected macro-, micro-, and non-nutrient elements in the leaves of A. glutinosa during the vegetation season were monitored in the Ore Mountains (Czech Republic), an area affected by extreme air pollution in the past. Decreased foliar content of N, P, K, and Cu, and increased content of Ca, Mn, Zn, and Al were observed; the content of other elements (S, Mg, Pb, and Cd) varied during the growing season or remained constant. From the viewpoint of nutrition, the content of N, S, Ca, and Mg macroelements was adequate; concentrations of P and K were low. Excessive amounts of Mn and Zn were measured, and the level of Cu was good. Non-nutrient elements Pb and Cd were present at the background level, and the level of Al was high. N/P, N/Ca, N/Mg, and Ca/Mg ratios were balanced, S/N value showed the lack of S, and N/K ratio indicated low content of K, which caused also suboptimal K/Ca and K/Mg values. The P/Al ratio varied from balanced to lower values. The content of individual elements and monitored changes were influenced by the amount of elements in the soil, moisture conditions, foliage phenology, and altitude.OA-hybri