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Integrated Harvesting of Medium Rotation Hybrid Poplar Plantations: Systems Compared
Full text linkIn this study, the authors provide a direct comparison made between whole tree-harvesting (WTH) and cut-to length (CTL) methods, which was conducted in two sites in the Slovak Republic and applied to poplar plantations. Both systems, WTH and CTL, have been employed at the highest mechanization level in two sites: "Nivky" and "Skalica". Two different strategies, namely, "mass handling" and "small-scale mechanization", have been used for WTH and CTL, respectively. The study results showed that the level of productivity (ODT SMH MINUS SIGN 1) in the felling operation was almost double for WTH than for the CTL method in Nivky (+84%) and more than double in Skalica (+113%). The extraction operation under WTH showed a productivity increase from one fifth (+20%) to more than double (104%) that of the CTL method in the Nivky and Skalica sites, respectively. Regarding cost-efficiency (EUR ODT MINUS SIGN 1), the WTH system offers a similar trend except with respect to extraction in Nivky (higher productivity site), in which the CTL extraction was 4.5% less expensive than the WTH extraction. The study results show that the mass-handling technique deployed in the WTH system offers very good performance in poorer plantations since the very small tree size and low-growing stock challenge the CTL system more than the WTH system. The total operation (felling, bucking, and extraction) costs (EUR ODT MINUS SIGN 1) recorded by the study in commercial conditions (as contractors perform for revenue purposes) were 32.50 and 45.80 EUR ODT MINUS SIGN 1 for CTL and 43.30 and 53.60 EUR ODT MINUS SIGN 1 for WTH for the higher-yield site (Nivky) and lower-yield site (Skalica), respectively. Regarding the WTH System, the researchers found that the drop in efficiency (and the consequent rise in the costs-per-ton of product) depends largely on the bucking phase conducted using the harvester at the landing of the stacked piles of interlocked trees. The main results of this study demonstrate the promising strategy of mass handling associated with the WTH system in medium rotation coppice (MRC) harvesting and shows that bucking is the weaker phase. Future efforts must be made to ensure feasibility of the "mass handling" strategy alongside the entire workflow by means of finding suitable mobile machinery that can delimb, debark, and crosscut tree bunches instead of single trees.O
The typology of countryside architectonical forms in South-Moravia, a region of Czechia
Full text linkThis article focuses on the typology of countryside architectonical forms in the region of South-Moravia in southeastern Czechia and on the expression of village identity through architecture in case study villages. Original folk architecture has been altered by new types of constructions built in rural areas since the 1950s, followed by a more recent wave of new architectural forms that have developed since the 1990s. The number of architectural types in case study villages was predominantly calculated using the panoramic sceneries on mapy.cz. The coefficients of countryside identity were allocated to architectural types based on basic folk house features. The value of countryside identity is higher in smaller villages except for suburbanized settlements of the regional capital of Brno.O
Mesenchymal stem cells of Oravka chicken breed: promising path to biodiversity conservation
Full text linkMesenchymal stem cells (MSCs) are multilineage cells able to differentiate into other cell types. MSCs derived from bone marrow or compact bones are the most accessible stem cells used in tissue engineering. Therefore, the aim of this study was to isolate, characterize and cryopreserve MSCs of endangered Oravka chicken breed. MSCs was obtained from compact bones of the femur and tibiotarsus. MSCs were spindle-shaped and were able to differentiate into osteo-, adipo-, and chondrocytes under the specific differentiation conditions. Furthermore, MSCs were positive for surface markers such as CD29, CD44, CD73, CD90, CD105, CD146 and negative for CD34CD45 by flow cytometry. Moreover, MSCs demonstrated high positivity of "stemness" markers aldehyde dehydrogenase, alkaline phosphatase as well as for intracellular markers vimentin, desmin, α-SMA. Subsequently, MSCs were cryopreserved using 10% dimethyl sulfoxide in liquid nitrogen. Based on the results from the viability, phenotype and ultrastructure assessment we can concluded that the MSCs were not negatively affected by the cryopreservation. Finally, MSCs of endangered Oravka chicken breed were successfully stored in animal gene bank, thus making them a valuable genetic resource.O
Stemflow infiltration hotspots near-tree stems along a soil depth gradient in a mixed oak–beech forest
Full text linkIn an upland forested micro-catchment during the growing season, we tested soil responses to precipitation events as well as soil water content (SWC). We asked ourselves if what is the difference of SWC response to precipitation events depending on the presence and proximity of a tree? The environmental heterogeneity of the small 7.5-ha headwater area was captured by soil probes at specific locations: (i) probe measurements of SWC at 10-, 30-, 60-, and 100-cm depths; (ii) resolution of near-tree (NT) and between-tree (BT) positions; and (iii) resolution of four slope classes. The results revealed significant differences between the hydrological responses of the soil. NT soils had faster infiltration but were also faster to dry out when compared to BT soils, which were less affected by the presence of trees. Water input threshold values, measured as the precipitation amount needed to cause a significant increase in SWC, were also significantly different, with NT positions always lower than BT positions. Total infiltration of the topmost NT and BT soil layers reached 185 and 156 mm during the study period, corresponding to 43% and 36% of the total 434 mm of precipitation, respectively. Infiltration into the deepest horizon was significantly higher in NT soils, where it reached 114 mm (26%) as opposed to 9 mm (2%) in BT soils, and was indicative of significant vertical hydraulic bypass flow in the proximity of trees. These observations contribute to better understanding the hydrological processes, their nonlinearity, and the expansion of conceptual hydrological models.OA-hybri
Redox regulation of chromatin remodelling in plants
Full text linkChanges in the cellular redox balance that occur during plant responses to unfavourable environmental conditions significantly affect a myriad of redox-sensitive processes, including those that impact on the epigenetic state of the chromatin. Various epigenetic factors, like histone modifying enzymes, chromatin remodelers, and DNA methyltransferases can be targeted by oxidative posttranslational modifications. As their combined action affects the epigenetic regulation of gene expression, they form an integral part of plant responses to (a)biotic stress. Epigenetic changes triggered by unfavourable environmental conditions are intrinsically linked with primary metabolism that supplies intermediates and donors, such acetyl-CoA and S-adenosyl-methionine, that are critical for the epigenetic decoration of histones and DNA. Here, we review the recent advances in our understanding of redox regulation of chromatin remodelling, dynamics of epigenetic marks, and the interplay between epigenetic control of gene expression, redox signalling and primary metabolism within an (a)biotic stress context.OA-hybri
Clavigeritae (Coleoptera, Staphylinidae, Pselaphinae) of the Arabian Peninsula with the description of a new species of Corynotopsis Jeannel, 1951 from Oman
Full text linkCorynotopsis omanicus sp. nov. of the myrmecophilous supertribe Clavigeritae is described from Oman. The type series was collected at night and the ant host species remains unknown. Corynotopsis scotti Jeannel, 1951 is newly recorded for Yemen, and Commatocerus concinnus Besuchet & Cuccodoro, 2011 for Oman. Lasius Fabricius, 1804 and Lepisiota Santschi, 1926 are, for the first time, determined as hosts of the latter species. The problematic taxonomic status of Corynotopsis scotti is discussed. A distribution map of all Clavigeritae known from the Arabian Peninsula is provided.O
Microplastics in animal nutrition: Occurrence, spread, and hazard in animals
Full text linkMicroplastics (MPs) are small plastic particles less than 5 mm in size, which have become a common environmental contaminant, spreading across terrestrial and aquatic ecosystems. Concerns have been raised about the impact of microplastics on animal health and nutrition due to their accumulation and potential transfer through the food chain. This review aims to summarize current knowledge about microplastics in animal nutrition, with a focus on their occurrence, sources, routes of exposure, and potential effects on animal physiology and metabolism. Animals can consume microplastics through a variety of routes, including direct consumption of contaminated food, uptake from contaminated water, and ingestion of organisms that have already consumed microplastics. MPs have been found in a variety of animal species, including fish, birds, mammals, and invertebrates, highlighting the possibility of widespread exposure and bioaccumulation. MPs can interact with the gastrointestinal tract after being ingested, potentially influencing nutrient absorption, gut microbiota composition, and overall digestive efficiency. MPs may also act as carriers for other chemical pollutants, potentially increasing their bioavailability and toxic effects on animals. While some studies indicate that microplastics may cause adverse effects in animals such as inflammation, oxidative stress, and disruption of endocrine functions, the overall health effects and long-term consequences of microplastic (MP) exposure in animal nutrition remain unknown. Furthermore, the review discusses the potential consequences of microplastics in animal-derived food products, considering the human health risks associated with their consumption.O
User-Driven Toponym Disambiguation Using Dialogue
Full text linkThe paper presents a novel method for processing information with a spatial component or querying geospatial databases. It proposes an interactive toponym disambiguation method tailored especially for dialogue systems and chatbots. The method exploits the interactive nature of dialogues to resolve ambiguity by using dialogue clarification techniques, i.e., asking users for additional information to perform disambiguation. The paper evaluates different questioning strategies and methods for selecting suitable toponym features to formulate disambiguation questions. A novel Equi-population partitioning method for selecting suitable toponym features in questions is presented as an approach outperforming other compared methods.OA-hybri
Long-term shift towards shady and nutrient-rich habitats in Central European temperate forests
Full text linkBiodiversity world-wide has been under increasing anthropogenic pressure in the past century. The long-term response of biotic communities has been tackled primarily by focusing on species richness, community composition and functionality. Equally important are shifts between entire communities and habitat types, which remain an unexplored level of biodiversity change. We have resurveyed > 2000 vegetation plots in temperate forests in central Europe to capture changes over an average of five decades. The plots were assigned to eight broad forest habitat types using an algorithmic classification system. We analysed transitions between the habitat types and interpreted the trend in terms of changes in environmental conditions. We identified a directional shift along the combined gradients of canopy openness and soil nutrients. Nutrient-poor open-canopy forest habitats have declined strongly in favour of fertile closed-canopy habitats. However, the shift was not uniform across the whole gradients. We conclude that the shifts in habitat types represent a century-long successional trend with significant consequences for forest biodiversity. Open forest habitats should be urgently targeted for plant diversity restoration through the implementation of active management. The approach presented here can be applied to other habitat types and at different spatio-temporal scales.OA-hybri
Gluten-free beer with unmalted millet
Full text linkThe possibility of using unmalted millet with the help of the enzymes for the production of gluten-free beer was investigated. The enzymes under different conditions were examined to completely saccharify the wort. The optimal conditions for enzymes activity were 85 oC 60 min for amylosubtilin and 60 oC 60 min for glucavamarin and β-glucanase. Since the gluten-free beverage has no colour, roasted buckwheat and boiled coffee as colourants were used. Sample with 30% roasted buckwheat showed good results in colouring the drink and had the best overall impression. Gluten-negative results were obtained for beer samples and all ingredients separately. Nevertheless, further improvements in brewing methods of gluten-free beer are needed. Almost all samples tasted more like cider than beer, without foam and with low pH values (3.5-3.8). The production of gluten-free beer provides an opportunity to attract new customers with gluten intolerance. Imperfect competition is a great advantage for manufacturers.OA-hybri