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Etiology and Molecular Mechanisms of PPP2R5D-Related Developmental Disorders
No full textThis dissertation is focused on unraveling the molecular mechanisms underlying PPP2R5D-related intellectual disability, known as Jordan\u27s Syndrome, using comprehensive proteomic and phosphoproteomic analysis. PPP2R5D encodes one of the 16 regulatory subunits of the protein phosphatase 2A (PP2A) holoenzyme, a key regulator involved in critical cellular processes such as cell growth, differentiation, and apoptosis. De novo single nucleotide mutations in PPP2R5D, including E198K and E420K, are believed to potentially disrupt PP2A function and lead to aberrant signaling. These mutations are definitively contributing to the pathogenesis of neurodevelopmental disorders such as Jordan\u27s Syndrome, which characterized by intellectual disability, seizures, hypotonia, autism spectrum disorders, global developmental delays, macrocephaly, and dysmorphic facial features. In this study, we utilized TMT-LC-MS³ technology and bioinformatic approaches to analyzed HEK293 and neural stem cell (NSC) models carrying specific mutations associated with Jordan’s Syndrome (PPP2R5D E198K and E420K), alongside PIK3CA-Related Overgrowth Spectrum (PROS)-related variants (PIK3CA H1047R and MTOR T1977I). The analysis revealed significant alterations in key signaling pathways, highlighting differential phosphorylation patterns in the PI3K-AKT and MEK-ERK-RSK axis, with downstream effectors such as RPS6 and eIF4B exhibiting variant-specific changes. Additionally, we identified a significant impact on spliceosome-associated proteins, linking aberrant splicing with the pathogenesis of neurodevelopmental disorders. Dysregulation of hnRNP A1 phosphorylation, which plays a role in Tau splicing, was particularly evident, suggesting a mechanistic connection between PPP2R5D dysfunction and neuronal impairments. This work provides insights into the molecular etiology of PPP2R5D-related neurodevelopmental disorders. Future directions include increasing phosphopeptide coverage, validating key proteins and pathways through biochemical assays, and exploring potential therapeutic targets to mitigate the effects of PPP2R5D mutations
Incorporating AI Processes in Human Subject Protection Programs
Get PDFhttps://jagworks.southalabama.edu/fric2024_presentations/1004/thumbnail.jp
University Libraries Newsletter - September 2023
Get PDFUniversity Libraries newsletter for September 2023.
Contents include: Lessha Coleman - new Assessment and Collections Librarian Traveling Exhibit at the Biomedical Library - Life and Limb: Toll of the American Civil War National Community Health Workers Week University Libraries Publications and Staff Updates USA Libraries Key Points and Happenings
USA Libraries Exhibits and Events: 100 Years of Hank Williams Exhibit Blakeley\u27s Historic Fields and Forest: A Plant Florilegium Studio 22 Textile Artists and Azalea City Quilters Art group - Science Matter
U2.31.1123_TA3 06.14.21 Prox. Humerus.ta3
No full textObjectives We estimate adult age distributions from Unar 1 and Unar 2, two late Umm an-Nar (2400-2100 BCE) tombs in the modern-day Emirate of Ras al-Khaimah, United Arab Emirates. These collective tombseach contained hundreds of skeletons in commingled, fragmented, and variably cremated states. Previous studies placed the vast majority of this mortuary community in a generalized “adult” category, as have most analyses of similar tombs from this period. We sought to test how adult age estimation methods compare in identifying young, middle, and old age individuals in commingled assemblages.
Materials and Methods We employed Transition Analysis 3 (TA3) and traditional age estimation methods to generate adult age distributions for each tomb. We compared these distributions between tomb contexts as well as by method.
Results Unar 1 and Unar 2 had similar adult age distributions within each method, but TA3 age distributions included significantly more middle and older adult individuals than those generated by traditional methods.
Discussion These results support findings of earlier iterations of Transition Analysis in regard to sensitivity in old adult age estimation, compared to traditional methods. Our findings indicate a potential use of TA3 in reconstructing age distributions and mortality profiles in commingled skeletal assemblages. Increasing our understanding of everyday life in the distant past necessitates better understandings of adult age, and here, we illustrate how age estimation method choice significantly changes bioarchaeological interpretations of aging in Bronze Age Arabia
U2.31.942_TA3 06.14.21 Prox. Humerus.ta3
No full textObjectives We estimate adult age distributions from Unar 1 and Unar 2, two late Umm an-Nar (2400-2100 BCE) tombs in the modern-day Emirate of Ras al-Khaimah, United Arab Emirates. These collective tombseach contained hundreds of skeletons in commingled, fragmented, and variably cremated states. Previous studies placed the vast majority of this mortuary community in a generalized “adult” category, as have most analyses of similar tombs from this period. We sought to test how adult age estimation methods compare in identifying young, middle, and old age individuals in commingled assemblages.
Materials and Methods We employed Transition Analysis 3 (TA3) and traditional age estimation methods to generate adult age distributions for each tomb. We compared these distributions between tomb contexts as well as by method.
Results Unar 1 and Unar 2 had similar adult age distributions within each method, but TA3 age distributions included significantly more middle and older adult individuals than those generated by traditional methods.
Discussion These results support findings of earlier iterations of Transition Analysis in regard to sensitivity in old adult age estimation, compared to traditional methods. Our findings indicate a potential use of TA3 in reconstructing age distributions and mortality profiles in commingled skeletal assemblages. Increasing our understanding of everyday life in the distant past necessitates better understandings of adult age, and here, we illustrate how age estimation method choice significantly changes bioarchaeological interpretations of aging in Bronze Age Arabia
Addressing Challenges & Reactions to COVID-19 in Academic Libraries
Get PDFThe unprecedented COVID-19 crisis has impacted many universities and academic libraries resulting in normal operations ceasing temporarily. Overall, academic libraries at the start of the COVID-19 pandemic immediately adjusted to the so-called new normal and accommodated disrupted work operations to operate successfully. This article discusses the procedures implemented, positive and negative reactions to these changes, and the challenges experienced at Alabama State University, Levi Watkins Learning Center, and other libraries. This article will also highlight implemented procedures carried out by other university libraries, including difficulties and successes that libraries experienced during peak periods of the COVID-19 pandemic. To conclude, academic libraries are challenged to adapt to new learning trends and the research needs of their campus communities to provide the best quality resources and services despite COVID-19
U1.31.122_TA3 06.22.21 Dist. Humerus.ta3
No full textObjectives We estimate adult age distributions from Unar 1 and Unar 2, two late Umm an-Nar (2400-2100 BCE) tombs in the modern-day Emirate of Ras al-Khaimah, United Arab Emirates. These collective tombseach contained hundreds of skeletons in commingled, fragmented, and variably cremated states. Previous studies placed the vast majority of this mortuary community in a generalized “adult” category, as have most analyses of similar tombs from this period. We sought to test how adult age estimation methods compare in identifying young, middle, and old age individuals in commingled assemblages.
Materials and Methods We employed Transition Analysis 3 (TA3) and traditional age estimation methods to generate adult age distributions for each tomb. We compared these distributions between tomb contexts as well as by method.
Results Unar 1 and Unar 2 had similar adult age distributions within each method, but TA3 age distributions included significantly more middle and older adult individuals than those generated by traditional methods.
Discussion These results support findings of earlier iterations of Transition Analysis in regard to sensitivity in old adult age estimation, compared to traditional methods. Our findings indicate a potential use of TA3 in reconstructing age distributions and mortality profiles in commingled skeletal assemblages. Increasing our understanding of everyday life in the distant past necessitates better understandings of adult age, and here, we illustrate how age estimation method choice significantly changes bioarchaeological interpretations of aging in Bronze Age Arabia
U1.31.211_TA3 06.22.21 Dist. Humerus.ta3
No full textObjectives We estimate adult age distributions from Unar 1 and Unar 2, two late Umm an-Nar (2400-2100 BCE) tombs in the modern-day Emirate of Ras al-Khaimah, United Arab Emirates. These collective tombseach contained hundreds of skeletons in commingled, fragmented, and variably cremated states. Previous studies placed the vast majority of this mortuary community in a generalized “adult” category, as have most analyses of similar tombs from this period. We sought to test how adult age estimation methods compare in identifying young, middle, and old age individuals in commingled assemblages.
Materials and Methods We employed Transition Analysis 3 (TA3) and traditional age estimation methods to generate adult age distributions for each tomb. We compared these distributions between tomb contexts as well as by method.
Results Unar 1 and Unar 2 had similar adult age distributions within each method, but TA3 age distributions included significantly more middle and older adult individuals than those generated by traditional methods.
Discussion These results support findings of earlier iterations of Transition Analysis in regard to sensitivity in old adult age estimation, compared to traditional methods. Our findings indicate a potential use of TA3 in reconstructing age distributions and mortality profiles in commingled skeletal assemblages. Increasing our understanding of everyday life in the distant past necessitates better understandings of adult age, and here, we illustrate how age estimation method choice significantly changes bioarchaeological interpretations of aging in Bronze Age Arabia
U2.31.274_TA3 06.22.21 Dist. Humerus.ta3
No full textObjectives We estimate adult age distributions from Unar 1 and Unar 2, two late Umm an-Nar (2400-2100 BCE) tombs in the modern-day Emirate of Ras al-Khaimah, United Arab Emirates. These collective tombseach contained hundreds of skeletons in commingled, fragmented, and variably cremated states. Previous studies placed the vast majority of this mortuary community in a generalized “adult” category, as have most analyses of similar tombs from this period. We sought to test how adult age estimation methods compare in identifying young, middle, and old age individuals in commingled assemblages.
Materials and Methods We employed Transition Analysis 3 (TA3) and traditional age estimation methods to generate adult age distributions for each tomb. We compared these distributions between tomb contexts as well as by method.
Results Unar 1 and Unar 2 had similar adult age distributions within each method, but TA3 age distributions included significantly more middle and older adult individuals than those generated by traditional methods.
Discussion These results support findings of earlier iterations of Transition Analysis in regard to sensitivity in old adult age estimation, compared to traditional methods. Our findings indicate a potential use of TA3 in reconstructing age distributions and mortality profiles in commingled skeletal assemblages. Increasing our understanding of everyday life in the distant past necessitates better understandings of adult age, and here, we illustrate how age estimation method choice significantly changes bioarchaeological interpretations of aging in Bronze Age Arabia
U2.31.343_TA3 06.22.21 Dist. Humerus.ta3
No full textObjectives We estimate adult age distributions from Unar 1 and Unar 2, two late Umm an-Nar (2400-2100 BCE) tombs in the modern-day Emirate of Ras al-Khaimah, United Arab Emirates. These collective tombseach contained hundreds of skeletons in commingled, fragmented, and variably cremated states. Previous studies placed the vast majority of this mortuary community in a generalized “adult” category, as have most analyses of similar tombs from this period. We sought to test how adult age estimation methods compare in identifying young, middle, and old age individuals in commingled assemblages.
Materials and Methods We employed Transition Analysis 3 (TA3) and traditional age estimation methods to generate adult age distributions for each tomb. We compared these distributions between tomb contexts as well as by method.
Results Unar 1 and Unar 2 had similar adult age distributions within each method, but TA3 age distributions included significantly more middle and older adult individuals than those generated by traditional methods.
Discussion These results support findings of earlier iterations of Transition Analysis in regard to sensitivity in old adult age estimation, compared to traditional methods. Our findings indicate a potential use of TA3 in reconstructing age distributions and mortality profiles in commingled skeletal assemblages. Increasing our understanding of everyday life in the distant past necessitates better understandings of adult age, and here, we illustrate how age estimation method choice significantly changes bioarchaeological interpretations of aging in Bronze Age Arabia