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Attitudes Towards Suicide: A Systematic Review of Evaluation Instruments in Different Settings
Get PDFBeyond the worst-case scenario: Inconsistencies in dust flammability parameter determination for organic solid fuels
Get PDFMinimizing detection bias of somatic mutations in a highly heterozygous oak genome
Get PDFSomatic mutations are particularly relevant for long-lived organisms. Sources of somatic mutations include imperfect DNA repair, replication errors, and exogenous damage such as ultraviolet radiation. A previous study estimated a surprisingly low number of somatic mutations in a 234-year-old individual of the pedunculate oak (Quercus robur), known as the Napoleon Oak. It has been suggested that the true number of somatic mutations was underestimated due to gaps in the reference genome and too conservative filtering of potential mutations. We therefore generated new high-fidelity long-read data for the Napoleon Oak (n = 12) to produce both a pseudo-haploid genome assembly and a partially phased diploid assembly. The high heterozygosity allowed for complete reconstruction of phased and gapless centromeres for 22 of the 24 chromosomes. On the other hand, the high heterozygosity posed challenges for short-read alignments. Use of only the pseudo-haploid assembly as a reference led to potential misalignments, while use of only the diploid assembly reduced variant detection sensitivity. Since most somatic mutations are layer-specific, the fraction of reads covering a specific somatic mutation is expected to be relatively low, even where all cells in a single layer contain a specific mutation. To address this challenge, we employed a read assignment strategy, selecting the appropriate reference sequence (pseudo-haploid or diploid) based on alignment score and mapping quality. Ultimately, we identified 198 high-confidence somatic mutations, compared with 17 somatic mutations identified before with the same set of short reads. Our approach thus increased the total estimated annual mutation rate by a factor of 5
Computational Characterization of Unsupported Au(I)···Ir(I) Metallophilic Interactions: Evidence for Strong Dispersive Stabilization
Get PDFMay Sulfites in Wine Affect Gut Microbiota? An In Vitro Study of Their Digestion and Interplay with Wine Polyphenols
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Plasma calprotectin as a severity biomarker in pediatric invasive Streptococcus pyogenes infections: insights from a multicenter immune profiling study during an outbreak
Get PDFMaking weight makes sense: relative performance gains after rapid weight loss in powerlifting: a randomized controlled trial
Get PDFGlobal epidemiology of high priority and pandemic Pseudomonas aeruginosa in pets, livestock, wild, and aquatic animals: a systematic review and meta-analysis
Get PDFEl escalofriante medio de comunicación que es la Naturaleza
Get PDFEn el presente texto se propone la hipótesis de la concepción de la Naturaleza como medio de comunicación. Atendiendo a la etimología del término «comunicación» y separándonos en este punto de la tesis de Marshall McLuhan, presentaremos su carácter bifaz: la obligación de participación en igualdad de condiciones. Tratando de animar el debate y convocando a nuevas interpretaciones, el objetivo final de nuestra lectura es la comprensión de la Naturaleza como agente político evitando el criterio antropocéntrico.This text proposes the hypothesis of the conception of Nature as a means of communication. Attending to the etymology of the term «communication» and separating ourselves at this point from Marshall McLuhan's thesis, we will present its bifacial character: the obligation of participation on equal terms. Trying to encourage the debate and calling for new interpretations, the final objective of our reading is the understanding of Nature as a political agent avoiding the anthropocentric criterion