Animo Repository - De La Salle University Research
Not a member yet
45334 research outputs found
Sort by
Screening the effects of camellia sinensis (L.) kuntze (Tea plant) matcha on the embryonic development of the chicken (Gallus gallus domesticus L.)
No full textTea is the most widely consumed beverage globally, and matcha, a powdered form of green tea derived from the tea plant, Camellia sinensis (L.) Kuntze is particularly known for its health benefits including their antioxidant properties. This study aimed to screen the potential effects of matcha on the growth and development of chicken embryos (Gallus gallus domesticus L.). A total of 40 fertilized eggs were divided into four groups of two control groups and two treatment groups receiving doses of 500 µg and 1,000 µg of matcha per egg, respectively. The embryos were subjected to morphometric analysis to determine even subtle changes in the anatomy, hence, any effect on the growth of the embryo. The body weight (in gms) and the following anatomical parameters (in mm) were considered: crown-to-rump length, beak length, neck length, wing length, thigh length, drumstick length, shank length, and third toe length. The findings showed that Treatment 1 administered with 500 µg of matcha per egg exhibited the highest average values for all the morphometric parameters compared to Treatment 2 administered with 1000 µg of matcha per egg relative to control groups. However, statistical analysis using one-way ANOVA did not reveal any significant differences across all the groups (p \u3e 0.05), indicating that the administered doses of matcha simply maintained normal chick embryo growth and development. The present study, including varied results from related studies, should prompt future researchers to investigate more on the effects of matcha with its bioactive constituents on avian embryonic development
Improving WSN-based dataset using data augmentation for TSCH protocol performance modeling
No full textThis study addresses the problem of inadequate datasets in Time-Slotted Channel Hopping (TSCH) protocol in Wireless Sensor Networks (WSN) by introducing a viable machine learning (ML) approach that explicitly tackles the limitations associated with the scarcity of data samples. The dataset employed in this research is derived from actual sensor node implementations, ensuring authenticity and relevance. To counteract overfitting, Variational Auto-Encoder (VAE) and Generative Adversarial Network (GAN) algorithms are utilized for data augmentation during the modeling phase, alongside the incorporation of Random Forest (RF) and Artificial Neural Network (ANN) algorithms. Results reveal a notable improvement in the performance of the ML models through the implementation of data augmentation techniques. A comparative analysis of various ML models underscores the superiority of the RF model, augmented by the GAN technique. This model exhibits enhanced predictive capabilities for TSCH latency, underscoring its efficacy in modeling network protocol performance
Assessment of free-living amoebae presence in tap water sources in a residential area in Tondo, Manila
No full textThis study investigated the presence of free-living amoebae (FLA) in tap water sources within Paradise Heights, Barangay 128, Tondo, Manila, a densely populated urban community with compromised sanitation and aging water infrastructure. Free-living amoebae such as Acanthamoeba spp., Naegleria spp., Entamoeba spp., and Balamuthia mandrillaris are known to cause serious and sometimes fatal infections in humans. The general problem of the study is that there is an insufficient amount of local public health data regarding the presence of free-living amoebae in urban water systems in the Philippines, due to the limited existing scientific investigations on FLA. The purpose of the study was to determine whether these amoebae are present in the tap water of a vulnerable urban community and to examine environmental conditions that could support their prevalence and distribution. A total of 48 tap water samples were collected from 16 residential buildings and analyzed through direct microscopy and culture techniques using non-nutrient agar plates seeded with Escherichia coli. The physicochemical parameters, including pH and temperature, were also measured on-site. Microscopic and culture analyses revealed no detectable trophozoites, cysts, or signs of FLA growth in any of the samples. The water samples exhibited neutral pH and moderate temperatures that were not particularly favorable for FLA proliferation. These findings indicate that the tap water in Paradise Heights showed no signs of FLA contamination during the sampling period. However, the study acknowledges that the absence of molecular diagnostic tools and the limited duration of sampling may have affected detection sensitivity. It is recommended that future studies incorporate more advanced methods such as PCR and conduct sampling across different timeframes to account for potential seasonal variation. The results highlight the importance of continuous water quality monitoring to safeguard public health in underserved communities
Screening the effects of calamansi (x Citrofortunella microcarpa (Bunge) Wijnands) peel extract on angiogenesis in duck embryo (Anas platyrhynchos domesticus)
No full textThe therapeutic potential of plant extracts, particularly belonging to the genus Citrus within the Rutaceae family, has been well-recognized due to their rich content of vitamins and bioactive compounds that offer significant health benefits. However, the specific effects of calamansi by-products on vascular development have not been thoroughly studied and remain underexplored. This study screened the effects of calamansi (x Citrofortunella microcarpa (Bunge) Wijnands) peel extracts on angiogenesis in the embryos of the Philippine Mallard Duck (Anas platyrhynchos domesticus) based on six key vascular parameters to evaluate blood vessel formation as an indicator of growth and development. The experimental design included two control groups 1 and 2, with no manipulations and with physiological saline solution, respectively, and three treatment groups administered with doses 1, 2, and 3, i.e., 200 µg/egg, 500 µg/egg, and 1000 µg/egg, respectively of the calamansi peel crude extract. The ImageJ Vessel Analysis and Angiogenesis Analyzer plugins were utilized to present blood vessel formation, growth extension, and complexity with the following vascular parameters: vascular density, number of segments, branches, peaces, meshes, and junctions. Results suggest a non-linear relationship, wherein samples treated with dose 2 showed the most favorable vascular development with increased vascular growth and complexity when compared to doses 1 and 3 and relative to the control group. In contrast, doses 1 and 3 led to decreased vascular parameters, suggesting potential inhibitory or toxic effects at suboptimal and excessive dosages. These findings align with the non-monotonic dose-response pattern, where intermediate doses yield optimal responses while extreme doses are less effective. This study provides a preliminary assessment of the potential effects of calamansi peel extract in promoting angiogenesis, emphasizing the need for further investigation