Animo Repository - De La Salle University Research
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Terrapiya
Terrapiya was formed in 2024 by a team of ABM students at De La Salle University - Manila to address the lack of accessible greenery in urban spaces. Specializing in customizable miniature terrariums, Terrapiya has grown from a school project into a promising venture, currently in the early commercialization stage with successful market testing and direct sales through bazaars and social media. Its advantage lies in customizable designs, locally sourced materials, and a strong sustainability ethos. The business possesses no proprietary rights but operates as a sole proprietorship to streamline decision-making processes under Rein Acuzar. She is supported by a proficient team comprising Ms. Lim, Mr. Tan, Ms. Dingsalsan, and Mr. Namuag, each with essential expertise in finance, marketing, human resources, and operations, respectively. Terrapiya operates in the eco-friendly gift and home décor industry, targeting urban, nature-oriented individuals who seek low-maintenance, personalized greenery. The products offer aesthetic, sustainable, and mental wellness benefits. Market entry is driven by pop-up bazaars and social platforms to boost engagement and visibility. Terrapiya competes with mass-market natural décor brands and hobbyist terrarium sellers such as Terramoss and Unbeleafable PH. Larger brands benefit from reach and recognition but often lack personalization. The business launched with an initial investment of ₱30,975.00 through self-financing, with each member contributing ₱6,195.00. For future operations, Terrapiya will rely on internal financing via reinvested profits and member contributions. Member investments will be returned along with divided profits following the Term 3 ABM Bazaar
Isolation, phenotypic, and genotypic characterization of Escherichia coli isolated from environmental wastewater
Antimicrobial resistance (AMR) is a pressing issue concerning public health as pathogens take up AMR genes (ARGs) that counter commonly available medications—prompting consistent monitoring. However, regular surveillance of AMR in the Philippines remains inadequate and primarily restricted to select tertiary hospitals and laboratories. This study sought to address the gap in surveillance within urban environments by collecting wastewater samples from three sites within De La Salle University Manila, an institution that represents an urban community equipped with various laboratories, classrooms, clinics, canteens, and offices. Focusing on Escherichia coli as an AMR indicator in environmental settings, 110 isolates were purified from the samples. After a series of phenotypic characterization methods, 58 presumptive E. coli isolates were identified and subjected to antibiotic susceptibility testing against imipenem, meropenem, ertapenem, cefotaxime, and levofloxacin following CLSI standards using the Kirby-Bauer disk diffusion method. Majority of the isolates were susceptible with the exception of the C135 isolate being intermediate to levofloxacin, and the cefotaxime-resistant A241 isolate. Focusing on AMR monitoring, whole genome sequencing was performed on the extracted DNA of A241 isolate to determine underlying genetic mechanisms regarding its resistance. Genome analysis revealed 56 ARGs, 25 mobile genetic elements (MGEs), and 163 virulence genes. The resistome revealed a variety of ARGs for different classes of antibiotics, however only one of them has been phenotypically confirmed as an active ARG—the blaCTX-M-15 gene identified as the genetic determinant of A241’s cefotaxime resistance—suggesting that the other ARGs are not highly expressed, differentially regulated, or dependent on other elements. Analysis of its mobilome revealed the presence of MGEs, specifically plasmids and insertion sequences, like the phage plasmid IncFIB(H89) that contains the blaCTX-M-15 gene which could accelerate the spread of AMR while the virulome disclosed potential pathogenicity mechanisms including secretion systems, nutrient-intake genes, and stress-related genes. The presence of a cefotaxime-resistant or ESBL-producing E. coli in urban wastewater could be an early indicator that AMR can spread in the university’s wastewater, but with one resistant isolate resulting from 58 isolates, the institution could be containing the wastewater adequately. Although cefotaxime-resistant E. coli in the Philippines has been documented in agricultural environments, this is the first recorded occurrence of cefotaxime-resistant E. coli in urban wastewater—highlighting a need for expanded AMR surveillance beyond clinical and agricultural settings as urban wastewater may serve as unmonitored reservoirs for ARG dissemination
The moderating role of sleep hygiene on eco-anxiety and academic performance of college students
This study investigates the moderating role of sleep hygiene on the relationship between eco-anxiety and academic performance among college students at De La Salle University-Manila. Sleep hygiene was obtained through the Sleep Hygiene Index (SHI)., Eco-anxiety, defined as the internal response to climate change and also interchangeable to climate change anxiety, was measured using the Climate Change Anxiety Scale (CAS). And academic performance was measured using self-reported cumulative grade point averages (cGPA). Results reveal that eco-anxiety positively predicts academic performance with P = 0.000; suggesting that higher levels of eco-anxiety correlates with better academic outcomes. Sleep hygiene however did not moderate the relationship between eco-anxiety and academic performance, showing no statistical significance with P = 0.089. This study highlights the potential and complex interactions between one\u27s physiology, mental health, environmental stressors, and academic success; which in turn emphasize the need for further research with larger sample sizes and comprehensive qualitative approaches to better understand their dynamics. The results contribute to the growing literature on the novel topic of climate-related mental health and its impact on an educational environment, offering insights for educators, mental health professionals, and policymakers aiming to support students in climate-vulnerable regions
Survey of canine babesiosis cases from veterinary clinics in selected areas in Metro Manila from June 2023 to June 2024
Canine babesiosis is a tick-borne disease caused by Babesia species, primarily transmitted by the bite of Rhipicephalus sanguineus (brown dog tick). This research study aimed to determine the number of cases within the selected veterinary clinics across different specific cities of Metro Manila, dating from June 2023 to June 2024, and to determine the various factors influencing the disease occurrence such as dog characteristics, diagnostic tools, and treatment strategies. A retrospective data collection was performed through interviews and surveys from veterinarians concerning their clinical records. Overall, there were 14 clinics who participated in the study; however, 8 of those were able to give an estimated number only. The remaining 6 clinics who gave an absolute number reported a total number of 227 cases encountered from June 2023 to June 2024. The survey found that cases peaked during the dry season of the country from March to May, aligning with increased tick activity affected by higher temperatures and humidity. From the survey results, long-haired dog breeds were more frequently affected, alongside male and adult dogs; thus, suggesting a link between behavioral, physiological, and environmental factors. In terms of diagnosis and treatment, the surveyed veterinary practitioners rely mainly on rapid serological test kits while treatment approaches can be varied based on the severity of the case. According to the survey, the most prescribed drugs were imidocarb and doxycycline, followed by combination therapies administering clindamycin and metronidazole for severe cases. Survey reports of observed potential drug resistance and reoccurrence, particularly in doxycycline and metronidazole, emphasize the need for standardized diagnostic and treatment protocols. Furthermore, the study highlights the importance of post-treatment monitoring, improved data storage and case tracking, as well as further research into drug efficacy, sex-based susceptibility, diagnostic accuracy, and disease prevalence to enhance disease management and prevention strategies.
Keywords: canine babesiosis, Babesia, Rhipicephalus sanguineus, Metro Manila, veterinary medicin
Ang Transdisiplinaryong Oryentasyon at Filipinolohiya bilang Metodolohiya ng Araling Filipino
Layunin ng papel na ito na ilatag ang pundasyon, mga teoretikal na konsiderasyon at acakademikong direksiyon ng iba’t ibang oryentasyon ng Araling Filipino (AF) bilang inter/multidisiplinaryo at transdisiplinaryong larangan. Binubuo ito ng tatlong bahagi—una, sinipat ang Araling Filipino bilang larangang may paksa, dalumat, metodolohiya at adhika; ikalawa, tinalakay nang masinsinan ang depinisyon at pagkakaiba ng tatlong oryentasyon; ikatlo’t huli, inilatag ang Filipinolohiya bilang mungkahing katawagan sa metodolohiya ng Araling Filipino. Inilagay ng mananaliksik/manunulat ang kaniyang tinig at karanasan sa ilang pag-aaral na ginawa at ginagawa tulad ng sanghiyang sa penomena ng Internet, pantawang pananaw bilang tawa at tuligsa, kritika bilang pagsusuri na/ng may sinasabi kasama ang iba pang pag-aaral ng mga iskolar bilang paghahalimbawa sa estado ng pananaliksik sa Araling Filipino. Sa kalaunan, ang intelektuwalisasyon ng wikang Filipino at sistematisasyon ng pananaliksik at metodolohiya sa pagbuo ng sangandiwa ng ating kaalaman ang sentral at pangmatagalang adhika ng Araling Filipino
Spoken Philippine English in Professional Contexts: Reflections on Research and the Need for a Targeted Corpus
This article reflects on nearly two decades of research into spoken communication using corpora, particularly within professional and workplace settings in the Philippines, such as call centers and related industries. Drawing on this extensive body of work, the article explores the potential of corpus-based approaches to enhance our understanding of spoken discourse. A framework for corpus-assisted spoken discourse analysis (Friginal, 2024a) is discussed as a foundation for examining professional communication. Building on these insights, the development of a targeted Spoken Philippine English Corpus (SPEC) is proposed, aiming to systematically document and analyze the unique features of spoken professional English in the Philippines. The article underscores the broader implications of this work for language teaching, assessment, and professional training, advocating for the critical role of spoken corpora in capturing and supporting the evolving dynamics of English in professional contexts
Interdisciplinary Comparison of Verb Complementation Patterns in Selected Nigerian University Course Textbooks: Insights from Humanities and Natural Sciences
This paper describes the variation in the distributions and use of verb complementation types and patterns in selected academic textbooks. The theoretical framework is Chomsky’s phrase structure grammar. The data, obtained from the first chapters, titled, ‘General introduction’ and ‘Atom and its structure’ of purposively selected English Studies and Physics textbooks, comprising 473 transitive verb phrases are manually analyzed. The findings reveal that three verb complementation types, mono-transitive, intensive, and complex-transitive, vary in patterns across the two textbooks with the mono-transitive V+NP and intensive V+NP patterns being dominant in English while the complex-transitive V+PP pattern dominates in Physics. The findings contribute to the description and understanding of discipline-specific verb complementation types and patterns. It concludes that the varying verb complementation types and patterns can instantiate disciplinary variation in academic textbooks. This has pedagogic implications for explicit and focused instruction on the discipline-specific verb complementation patterns that would facilitate Nigerian students’ awareness of the different verb complementation types and patterns and the development of competence in English for general and specific academic purposes. The paper recommends a more exhaustive study of the discipline-specific verb complementation patterns would tremendously provide more pedagogic insights relating to students’ learning of English for general and specific academic purposes. Such knowledge of verb complementation patterns in academic textbooks undergirds their efficiency in the students’ comprehension and production of academic texts. The paper suggests further research in this area relating to disciplinary and subject textbook variability as such has pedagogic implications
Islamic Premarital Education in Malaysia Through the Lens of Moral Education
Premarital education in Malaysia is recognized as exemplary, given that it has become a model for the development of similar endeavors in other countries in Southeast Asia. Several studies have been conducted to evaluate the provision of premarital education in Malaysia. However, it was found that an evaluation through a moral-educational framework was missing, even though premarital education should be viewed as part of moral education. To fill this gap, this paper offers an analysis and assessment of Islamic premarital education in Malaysia through Sanger and Osguthorpe’s (2005) two-level framework. Data were collected from official documents, non-participant observation, and in-depth interviews with administrators, instructors, and class participants at the Premarital Course Centre TTDI, Kuala Lumpur. The findings showed the framework to be a powerful analytical tool. A clear picture was shown of Islamic premarital education as moral education, which at the same time threw light on its inconsistencies between (a) moral content, moral assumptions, and Islamic moral teachings; (b) moral content and programmatic ends; and (c) the method of instruction, psychological assumptions, and programmatic ends. These inconsistencies provide a basis for further development of Islamic premarital education as effective moral education in the future
A Comparative Analysis of Maceration Extraction Methods and Their Efficacy in Biofilm Inhibition by Annona muricata Leaf Extract Against Bacillus subtilis
Biofilms, ubiquitous microbial communities encased in a self-produced matrix, pose a significant threat to human health, industrial processes, and food safety. While synthetic antimicrobials are widely used, concerns about resistance and environmental impact drive the search for natural alternatives. Guyabano (Annona muricata), a tropical fruit known for its antimicrobial properties, shows potential as a natural antibiofilm agent. This study aimed to compare the impact of three different maceration extraction methods (aqueous, ethanolic, and methanolic) on the antibiofilm efficacy of A. muricata leaf extract against Bacillus subtilis biofilm. Each extract was subjected to a 96-well microplate biofilm inhibition assay, measuring optical density (OD) at 600 nm to quantify biofilm formation. Serial dilution and colony-forming unit (CFU) counting were used to assess bacterial viability. The study found that all three extracts demonstrated notable biofilm inhibition, with the highest inhibition observed at a 10−7dilution for ethanolic extract (89.56%). However, the extracts exhibited inconsistent antibacterial activity, with bacterial colonies too many to count (TMTC) at higher dilutions. Statistical analysis revealed no significant difference in the efficacy among the maceration extraction methods (p = 0.67), confirming that all are equally viable for obtaining bioactive compounds from A. muricata. Regardless of the method used, the extracts effectively inhibited B. subtilis biofilm formation
Diminishing marginal utility of technological devices toward academic performance in mathematics, reading, and science
The Philippines, in its pursuit of aligning its education system with global standards, has participated in the Program for International Stu- dent Assessment (PISA) which evaluates 15-year-olds\u27 reading, scien- tific, and mathematical proficiency. However, the 2022 PISA report ranked Filipino learners among the lowest five in reading, science, and mathematics. This study explores how ownership of technological de- vices influences student performance in these domains. Using Ordinal Logistic Regression, we analyze the 2022 PISA ordinal data for 7608 Filipino students. Results show a diminishing marginal return on aca- demic achievement as device ownership increases. While initial access to technology boosts performance, the effect weakens as students own more devices. This trend is stronger among learners without siblings and persists regardless of internal or external digital distractions. Find- ings emphasize the need for balanced digital engagement. Rather than restricting access or full enablement, families and policymakers should focus on strategic technology use to enhance education, aligning with Sustainable Development Goals for quality learning